DE602006015852D1 - PROCESS FOR PREPARING POLY (ALKOXYLATED) OLIGONUCLEOTIDES - Google Patents

PROCESS FOR PREPARING POLY (ALKOXYLATED) OLIGONUCLEOTIDES

Info

Publication number
DE602006015852D1
DE602006015852D1 DE602006015852T DE602006015852T DE602006015852D1 DE 602006015852 D1 DE602006015852 D1 DE 602006015852D1 DE 602006015852 T DE602006015852 T DE 602006015852T DE 602006015852 T DE602006015852 T DE 602006015852T DE 602006015852 D1 DE602006015852 D1 DE 602006015852D1
Authority
DE
Germany
Prior art keywords
poly
oligonucleotide
alkoxylated
oligonucleotides
preparing poly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006015852T
Other languages
German (de)
Inventor
Nanda Dulal Sinha
Satya Kuchimanchi
Saied Shaikh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Denko Avecia Inc
Original Assignee
Avecia Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avecia Biotechnology Inc filed Critical Avecia Biotechnology Inc
Publication of DE602006015852D1 publication Critical patent/DE602006015852D1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)

Abstract

A process for the preparation of a poly(alkoxylated) oligonucleotide is provided. The process comprises reacting an oligonucleotide which has been purified by ultrafiltration with a poly(alkoxide) thereby to form a poly(alkoxylated) oligonucleotide. The poly(alkoxyalted) oligonucleotide may be separated from non-poly(alkoxyalted) oligonucleotide by ultrafiltration under denaturing conditions, such as the presence of organic solvents, for example, ethanol; the presence of urea; the addition of chaotropic salts, for example perchlorate and guanidinium salts; the presence of formamide; and the application of heat, for example a temperature of up to about 70° C.
DE602006015852T 2005-12-07 2006-11-20 PROCESS FOR PREPARING POLY (ALKOXYLATED) OLIGONUCLEOTIDES Active DE602006015852D1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74289705P 2005-12-07 2005-12-07
PCT/GB2006/004324 WO2007066069A2 (en) 2005-12-07 2006-11-20 Process for the preparation of poly(alkoxylated) oligonucleotides

Publications (1)

Publication Number Publication Date
DE602006015852D1 true DE602006015852D1 (en) 2010-09-09

Family

ID=38123246

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006015852T Active DE602006015852D1 (en) 2005-12-07 2006-11-20 PROCESS FOR PREPARING POLY (ALKOXYLATED) OLIGONUCLEOTIDES

Country Status (6)

Country Link
US (1) US8163891B2 (en)
EP (1) EP1963351B1 (en)
AT (1) ATE475666T1 (en)
CA (1) CA2629753A1 (en)
DE (1) DE602006015852D1 (en)
WO (1) WO2007066069A2 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1239390A (en) * 1967-11-08 1971-07-14
US3654682A (en) * 1970-09-01 1972-04-11 Edward H Newbould Tool holder
US3891195A (en) * 1974-05-14 1975-06-24 Lif O Gen Inc Cutting-welding torch
SE509931C2 (en) * 1996-09-27 1999-03-22 Seco Tools Ab Swivel mill, swivel head and method of mounting a removable swivel head on a shaft for a swivel

Also Published As

Publication number Publication date
EP1963351B1 (en) 2010-07-28
WO2007066069A2 (en) 2007-06-14
CA2629753A1 (en) 2007-06-14
US8163891B2 (en) 2012-04-24
WO2007066069A3 (en) 2007-09-20
US20100029924A1 (en) 2010-02-04
EP1963351A2 (en) 2008-09-03
ATE475666T1 (en) 2010-08-15

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