DE602005026221D1 - Gen-fallen-kassette für eine gerichtete und ungerichtete konditionelle geninaktivierung - Google Patents

Gen-fallen-kassette für eine gerichtete und ungerichtete konditionelle geninaktivierung

Info

Publication number
DE602005026221D1
DE602005026221D1 DE602005026221T DE602005026221T DE602005026221D1 DE 602005026221 D1 DE602005026221 D1 DE 602005026221D1 DE 602005026221 T DE602005026221 T DE 602005026221T DE 602005026221 T DE602005026221 T DE 602005026221T DE 602005026221 D1 DE602005026221 D1 DE 602005026221D1
Authority
DE
Germany
Prior art keywords
gene trap
mutations
cassette
cassettes
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005026221T
Other languages
English (en)
Inventor
Melchner Harald Von
Frank Schnuetgen
Wolfgang Wurst
Patricia Ruiz
Silke De-Zolt
Thomas Floss
Jens Hansen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum Muenchen Deutsches Forschung De
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Frankgen Biotechnologie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP04028194A external-priority patent/EP1662005A1/de
Priority claimed from EP05103092A external-priority patent/EP1715053A1/de
Application filed by Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH, Max Planck Gesellschaft zur Foerderung der Wissenschaften eV, Frankgen Biotechnologie AG filed Critical Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Publication of DE602005026221D1 publication Critical patent/DE602005026221D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Tape Cassette Changers (AREA)
DE602005026221T 2004-11-26 2005-11-28 Gen-fallen-kassette für eine gerichtete und ungerichtete konditionelle geninaktivierung Active DE602005026221D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP04028194A EP1662005A1 (de) 2004-11-26 2004-11-26 "Gene Trap" Vektoren, die mindestens einen Enhancer enthalten und die für zufälliges und gezieltes "Gene Trapping" geeignet sind
EP05103092A EP1715053A1 (de) 2005-04-18 2005-04-18 Gen-Fallen-Kassette für eine gerichtete und ungerichtete konditionelle Geninaktivierung
PCT/EP2005/056282 WO2006056617A1 (en) 2004-11-26 2005-11-28 Gene trap cassettes for random and targeted conditional gene inactivation

Publications (1)

Publication Number Publication Date
DE602005026221D1 true DE602005026221D1 (de) 2011-03-17

Family

ID=35922887

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005026221T Active DE602005026221D1 (de) 2004-11-26 2005-11-28 Gen-fallen-kassette für eine gerichtete und ungerichtete konditionelle geninaktivierung

Country Status (7)

Country Link
US (1) US20090113561A1 (de)
EP (1) EP1815001B8 (de)
AT (1) ATE497541T1 (de)
CA (1) CA2589208A1 (de)
DE (1) DE602005026221D1 (de)
DK (1) DK1815001T3 (de)
WO (1) WO2006056617A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8058509B2 (en) 2005-12-21 2011-11-15 Pioneer Hi-Bred International, Inc. Methods and compositions for in planta production of inverted repeats
DK3147362T3 (en) * 2009-10-29 2019-04-08 Regeneron Pharma Multifunctional alleles
AU2012100752A4 (en) * 2009-10-29 2012-06-21 Regeneron Pharmaceuticals, Inc. Multifunctional alleles
CA2782032A1 (en) 2009-11-25 2011-06-03 Laura Schebelle Conditional expression of transgenes in vivo
US8852930B2 (en) 2010-02-09 2014-10-07 The Trustees Of Columbia University In The City Of New York In vivo gene regulation by the combination of knock-in-tetO sequence into the genome and tetracycline-controlled trans-suppressor (tTS) protein
RU2664232C2 (ru) 2011-08-05 2018-08-15 Регенерон Фармасьютикалз, Инк. Мыши с гуманизированной универсальной легкой цепью
EP2599859A1 (de) 2011-11-30 2013-06-05 IMBA-Institut für Molekulare Biotechnologie GmbH Haploidzellen
EP2743345A1 (de) 2012-12-13 2014-06-18 IMBA-Institut für Molekulare Biotechnologie GmbH Dreidimensionale heterogen differenzierte Gewebekultur
MX2016009243A (es) * 2014-01-14 2017-03-08 Lam Therapeutics Inc Métodos de mutagénesis.
CA3018958A1 (en) 2016-02-29 2017-09-08 Wei Weng Dividing of reporter proteins by dna sequences and its application in site specific recombination
KR102630357B1 (ko) * 2017-02-17 2024-01-30 론자 리미티드 단백질 발현이 어려운 다중-부위 ssi 세포
KR102656200B1 (ko) 2017-06-16 2024-04-12 아이엠비에이 - 인스티튜트 퓌어 몰레쿨라레 바이오테크놀로지 게엠베하 혈관 오가노이드, 상기 오가노이드의 제조 및 사용 방법
WO2019048689A1 (en) 2017-09-11 2019-03-14 Imba - Institut Für Molekulare Biotechnologie Gmbh TUMOR ORGANOID MODEL
CN112391366B (zh) * 2019-08-16 2022-10-14 华东师范大学 光诱导激活的Dre重组系统

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6808921B1 (en) * 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US7449179B2 (en) * 2000-11-16 2008-11-11 Cornell Research Foundation, Inc. Vectors for conditional gene inactivation
US7074611B2 (en) * 2001-04-27 2006-07-11 Gie-Cerbn, Centre Europeen De Recherche En Biologie Et En Medecine (Gie) Method for the stable inversion of DNA sequence by site-specific recombination and DNA vectors and transgenic cells thereof
US7625755B2 (en) * 2003-05-30 2009-12-01 Wyeth Conditional knockout method for gene trapping and gene targeting using an inducible gene silencer

Also Published As

Publication number Publication date
DK1815001T3 (da) 2011-05-16
US20090113561A1 (en) 2009-04-30
EP1815001A1 (de) 2007-08-08
ATE497541T1 (de) 2011-02-15
EP1815001B8 (de) 2011-03-23
WO2006056617A1 (en) 2006-06-01
EP1815001B1 (de) 2011-02-02
CA2589208A1 (en) 2006-06-01

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: HELMHOLTZ ZENTRUM MUENCHEN DEUTSCHES FORSCHUNG, DE

Owner name: MPG MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER, DE