DE60038496D1 - PLANTS WITH REDUCED ACTIVITY IN TWO OR MORE STRENGTH-MODIFYING ENZYMES - Google Patents

PLANTS WITH REDUCED ACTIVITY IN TWO OR MORE STRENGTH-MODIFYING ENZYMES

Info

Publication number
DE60038496D1
DE60038496D1 DE60038496T DE60038496T DE60038496D1 DE 60038496 D1 DE60038496 D1 DE 60038496D1 DE 60038496 T DE60038496 T DE 60038496T DE 60038496 T DE60038496 T DE 60038496T DE 60038496 D1 DE60038496 D1 DE 60038496D1
Authority
DE
Germany
Prior art keywords
starch
plants
freeze
strength
thaw
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60038496T
Other languages
German (de)
Other versions
DE60038496T2 (en
Inventor
Stephen Alan Jobling
Gerhard Peter Schwall
Roger John Westcott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brunob II BV
Original Assignee
National Starch and Chemical Investment Holding Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Starch and Chemical Investment Holding Corp filed Critical National Starch and Chemical Investment Holding Corp
Application granted granted Critical
Publication of DE60038496D1 publication Critical patent/DE60038496D1/en
Publication of DE60038496T2 publication Critical patent/DE60038496T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Disclosed is potato starch which, when in native form extracted from a potato plant, exhibits freeze/thaw stability such that a 1 %w/v aqueous suspension of the starch has an absorbance at 700nm wavelength of less than 1.2 units following 4 freeze/thaw cycles of freezing at -70 DEG C overnight and thawing at room temperature for at least 2 hours; together with a method of altering the starch content of a plant; and altered plants, especially altered plants which contain freeze/thaw stable starch. Further disclosed is waxy (i.e. low amylose) starch having reduced gelatinization onset and swelling temperatures.
DE60038496T 1999-09-15 2000-09-13 PLANTS WITH REDUCED ACTIVITY IN TWO OR MORE STRENGTH-MODIFYING ENZYMES Expired - Lifetime DE60038496T2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9921830 1999-09-15
GBGB9921830.7A GB9921830D0 (en) 1999-09-15 1999-09-15 Plants having reduced activity in two or more starch-modifying enzymes
PCT/GB2000/003522 WO2001019975A2 (en) 1999-09-15 2000-09-13 Plants having reduced activity in two or more starch-modifying enzymes

Publications (2)

Publication Number Publication Date
DE60038496D1 true DE60038496D1 (en) 2008-05-15
DE60038496T2 DE60038496T2 (en) 2008-12-24

Family

ID=10860979

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60038496T Expired - Lifetime DE60038496T2 (en) 1999-09-15 2000-09-13 PLANTS WITH REDUCED ACTIVITY IN TWO OR MORE STRENGTH-MODIFYING ENZYMES

Country Status (9)

Country Link
EP (1) EP1212440B1 (en)
JP (1) JP2003509047A (en)
AT (1) ATE391184T1 (en)
AU (1) AU7031000A (en)
CA (1) CA2388364A1 (en)
DE (1) DE60038496T2 (en)
DK (1) DK1212440T3 (en)
GB (1) GB9921830D0 (en)
WO (1) WO2001019975A2 (en)

Families Citing this family (177)

* Cited by examiner, † Cited by third party
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