DE392300C - Blood counting device with adjustable chamber height and replaceable mesh micrometer plate - Google Patents

Description

Blood cell counting device with adjustable chamber height and exchangeable Mesh micrometer wafers. The general way of counting blood cells is taking place himself in such a way that that affects its content of red and white blood cells The blood to be examined is first measured very precisely in pipettes and with suitable liquids is brought to a certain degree of dilution. After this the blood prepared in this way is intimately mixed with the dilution liquid a drop of this mixture on the mesh micrometer plate of the counting chamber of the device brought.

With the devices that have been used up to now, the difference in height is now the outer lamella to the inner one with a micrometer mesh. Young provided, precisely matched to the respective chamber height set for calculating the volume. If you want to use a larger chamber height, like this for measuring white blood cells is required, one is forced to use a second apparatus or with one corresponding chamber, which sometimes also requires a different apparatus work.

The object of the present invention now provides an apparatus represents, in which the chamber height is variable and precisely adjustable, so that this Apparatus is suitable for all such measurements. In addition, this - Apparatus all existing network divisions are used. the Invention is shown in one embodiment in the drawing in double size, namely Fig. 1 shows a plan view, Fig. 2 shows a longitudinal section, Fig. 3 shows a micrometer plate with two network divisions, which are separated by a channel, Fig. 4. an apparatus with two adjustable chamber heights and exchangeable micro rivet plates.

The instrument consists of the slide a, which is made of metal or otherwise suitable unbreakable material is made. Of the-. same carries in in the middle an annular or angular socket b, into which the Netzmikro-Z, c such is inserted that the same by two pressure screws located on the right and left d is held against the horizontal surface e of the socket using a spring section f a pressure exactly parallel to the axis of the mesh micrometer plate exercise.

The replacement of the micrometer plate c on the surface is provided with a corresponding network division, is done by loosening the pressure screws d.

The slide is now also in the center with a thread provided by o, 25 min pitch, in which a ring nut g is screwed, the on its outward sloping surface with one to be determined during the adjustment Index line 1i. is provided, with which you can check the chamber height at any time can make.

Now rests on the ring nut g above (read cover slip 7, the a double spring k is pressed on. In the previous apparatus, the cover slip was pressed with the finger, which led to different measurement results.

The ring nut is now matched so that if the zero mark is the same with which the division of the slide coincides with cover slips and micrometer plates touch exactly with their flat surfaces.

If you screw it onto the counting net after applying the blood drop the ring nut to the left, so the Hoblrauin above the counting net represents 1, the upper surface of which is delimited by the underside of the cover glass, the actual Counting chamber, the height of which is as accurate as possible on the graduated slide is adjustable in hundredths of a millimeter.

After the sedimentation of the blood cells and the diluted blood solution on the counting network can be done at r2o to zoo times microscopic magnification the counting of the blood elements located on your counting network can be carried out.

There is a free one between the micrometer plate and the ring nut Space ;; i, which serves to accommodate the excess amount of liquid.

The apparatus has the great advantage that it always has the applied micrometer division exchanged for another Werder, can and the respective required chamber height-on the most accurate is adjustable. The apparatus also has the advantage that the mesh micrometer plates be held by a suitable clamping device, while with the previous The putty-up platelets can easily be cleaned by the apparatus used liquids, rendering the device unusable, moreover The accuracy of the chamber height is often determined by the strength of the putty applied influenced.

Claims (1)

PATEN T-AxSPRL C HE: T. Blood cell counting device with adjustable chamber height and exchangeable mesh micrometer plate, characterized by a slide (a) made of metal or other suitable unbreakable material, which is provided with a holder (b) in the middle., in which the net micrometer plate (c) is inserted in such a way that it is held by two pressure screws (d) which exert a pressure exactly parallel to the axis of the same against the horizontal surface (e) of the socket using a spring cut (f). z. Blood cell counting device according to claim i, characterized in that the object carrier (a) is provided in the middle with a fine thread into which an annular nut (g) is screwed, on the upper surface of which a cover slip (i) rests, the one between the lower The surface of the cover slip and the upper cavity (1) located on the mesh micrometer plate represents the actual counting chamber, the height of which can be adjusted with the greatest possible precision by means of an index line by turning the ring nut on the graduated slide, and the free space between the micrometer plate and the ring nut ( z: z) serves as a protection channel.