DE3909442A1 - Microtome cutting method for positive detection of cellular reactions - using intra=ocular lens implants which do not dissolve but which are preserved, enabling position of connection region to be detected - Google Patents
Microtome cutting method for positive detection of cellular reactions - using intra=ocular lens implants which do not dissolve but which are preserved, enabling position of connection region to be detectedInfo
- Publication number
- DE3909442A1 DE3909442A1 DE3909442A DE3909442A DE3909442A1 DE 3909442 A1 DE3909442 A1 DE 3909442A1 DE 3909442 A DE3909442 A DE 3909442A DE 3909442 A DE3909442 A DE 3909442A DE 3909442 A1 DE3909442 A1 DE 3909442A1
- Authority
- DE
- Germany
- Prior art keywords
- dissolve
- preserved
- detected
- intra
- connection region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007943 implant Substances 0.000 title claims abstract description 6
- 238000000034 method Methods 0.000 title claims abstract description 5
- 238000006243 chemical reaction Methods 0.000 title description 3
- 230000001413 cellular effect Effects 0.000 title description 2
- 238000005520 cutting process Methods 0.000 title description 2
- 238000001514 detection method Methods 0.000 title 1
- 238000007447 staining method Methods 0.000 claims description 2
- 238000010186 staining Methods 0.000 claims 1
- 210000001508 eye Anatomy 0.000 abstract description 7
- 230000007170 pathology Effects 0.000 abstract description 2
- 210000005252 bulbus oculi Anatomy 0.000 abstract 1
- 238000004040 coloring Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N chloroform Substances ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 229920002845 Poly(methacrylic acid) Polymers 0.000 description 1
- 240000007313 Tilia cordata Species 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 239000010627 cedar oil Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 231100000255 pathogenic effect Toxicity 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/14—Eye parts, e.g. lenses, corneal implants; Implanting instruments specially adapted therefor; Artificial eyes
- A61F2/16—Intraocular lenses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F9/00—Methods or devices for treatment of the eyes; Devices for putting-in contact lenses; Devices to correct squinting; Apparatus to guide the blind; Protective devices for the eyes, carried on the body or in the hand
- A61F9/007—Methods or devices for eye surgery
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Abstract
Description
Die Erfindung gehört zur Augenpathologie, wie sich aus dem folgenden ergibt:The invention belongs to eye pathology, as can be seen from the results in the following:
An der Oberfläche und in der weiteren Nachbarschaft der verschiedenen Teile intraokularer Linsenimplantate treten häufig pathologische Vorgänge auf, die bisher u. a. deshalb nicht mit Sicherheit als Reaktionen auf das Kunstlinsenim plantat nachzuweisen waren, weil die Berührungsstellen des kranken Gewebes mit der Kunstlinse noch nicht mikroskopisch sichtbar gemacht werden konnten. Die Sichtbarmachung der tatsächlichen Berührung von Linse und Gewebe im Mikroskop setzt voraus, daß das Präparat nichr nur das kranke Gewebe, sondern auch den mitgeschnittenen Kunstlinsenteil enthält. On the surface and in the wider neighborhood of the different parts of intraocular lens implants often pathological processes that have hitherto a. that's why not with certainty as reactions to the artificial lens were to be demonstrated because the contact points of the not yet microscopic with the artificial lens could be made visible. The visualization of the actual contact of lens and tissue in the microscope assumes that the preparation is not just the diseased tissue, but also contains the recorded artificial lens part.
Der aus Polymethacrylsäuremethylester (PMMA) bestehende Kunststofflinsenanteil des Mikrotomschnitts wird bei den herkömmlichen Färbeverfahren durch das dort verwendete Xylol aufgelöst. Dadurch erscheint das intraokulare Lindenimplan tat (IOL) im histologischen Präparat optisch als leere Flä che. Da die Linse nicht sichtbar ist, kann man deren Berüh rung mit dem kranken Gewebe nicht sehen. Einen Ursachenzu sammenhang zwischen Berührung und Entzündung konnte man da her bislang mangels anderer exakter Nachweise nur vermuten.The one consisting of polymethacrylic acid methyl ester (PMMA) Plastic lens portion of the microtome section is used in the conventional dyeing process using the xylene used there dissolved. This causes the intraocular linden implant to appear did (IOL) optically in the histological preparation as an empty area che. Since the lens is not visible, you can touch it with the diseased tissue. A cause there was a connection between touch and inflammation hitherto only suspect in the absence of other precise evidence.
Die räumliche Zuordnung des IOL zum entzündeten Gewebe konnte nur dann sichtbar und damit pathologisch und diagnostisch nachweisbar werden, wenn eine Färbemethode für den Mikrotom schnitt gefunden wurde, die die mitgeschnittene IOL nicht zerstört.The spatial assignment of the IOL to the inflamed tissue could only then visible and thus pathological and diagnostic become detectable if a staining method for the microtome the recorded IOL was not found destroyed.
Die Aufgabe wird durch folgendes, hier zum Patent angemelde tes Verfahren gelöst:The task is patented by the following, here Process solved:
- 1. Das enukleierte Auge wird zunächst 72 Stunden in 10% igem Formalin (Merck) fixiert.1. The enucleated eye is initially 72 hours in 10% formalin (Merck) fixed.
- 2. Vor der weiteren Aufbereitung im Autotechnicon (aufstei gende Alkoholreihe - Chloroform - Histowachs) werden die Augen 24 Stunden gewässert. 2. Before further processing in the Autotechnicon (ascending alcohol series - chloroform - histo wax) Eyes watered for 24 hours.
- 3. Vor der Eingabe in das Autotechnicon wird der Bulbus mit einer Kanüle in der hinteren Hälfte eröffnet, damit alle Präpariermittel auch das Augeninnere erreichen.3. Before entering into the Autotechnicon, the globe is included a cannula opened in the back half so everyone Preparations also reach the inside of the eyes.
- 4. Im Autotechnicon wird das Intermedium Chloroform durch Zedernholzöl ersetzt. Die Verweilsdauer darin ist auf zwei mal vierundzwanzig Stunden zu erhöhen.4. In the Autotechnicon, the intermediate medium is chloroform Cedarwood oil replaced. The dwell time in it is on increase two times twenty-four hours.
- 5. Nach Halbierung des Bulbus oder Anbringen einer Öffnung im Bulbus wird dieser in Histowachs eingebettet. Beim Einbetten werden die Augen nach ihrer Längsachse ausge richtet.5. After halving the globe or making an opening in the bulb it is embedded in histo wax. At the Embed the eyes out along their long axis judges.
- 6. Dann werden 5-my-dicke Mikrotomschnitte angefertigt, die auf mit Eiweißglycerin eingestrichene Objektträger im Wasserbad aufzuziehen und anschließend zu strecken sind. Das Schneiden erfolgt nicht ganz bis zur Mitte der IOL, da sonst die Gefahr besteht, daß das IOL aus dem Ein bettmaterial herausgerissen wird.6. Then 5 micron thick microtome sections are made on slides coated with protein glycerin in the Pull up the water bath and then stretch it. The cutting is not done all the way to the middle of the IOL, otherwise there is a risk that the IOL from the one bed material is torn out.
- 7. Nach dem Strecken werden die Präparate mit der Objekt seite nach unten für ca. 18 Stunden auf Filterpapier in den Wärmeschrank bei 56°C gelegt, damit schon jetzt ein Teil des Histowachses entfernt wird. Das restliche Wachs wird durch Einlegen der Präparate für 6 Stunden in Benzin herausgelöst.7. After stretching, the specimens with the object side down for about 18 hours on filter paper put the oven at 56 ° C, so now part of the histo wax is removed. The rest Wax is in for 6 hours by placing the preparations in Petrol extracted.
- 8. Sodann werden die absteigende Alkoholreihe bis Alkohol abs. und die eigentlichen Färbelösungen (PAS oder H.E.) durchlaufen.8. Then the descending alcohol row to alcohol Section. and the actual staining solutions (PAS or H.E.) run through.
- 9. Vor dem Eindecken mit Eukitt werden die aus dem Alkohol abs. kommenden Präparate vorsichtig einen Augenblick lang in Xylol getaucht, um die Verträglichkeit des Ge webeschnittes mit dem Einschlußmittel Eukitt sicherzu stellen.9. Before covering with Eukitt, the alcohol is removed Section. coming preparations carefully for a moment long immersed in xylene to check the compatibility of the Ge weave cut with the inclusion agent Eukitt put.
Vorteile erwachsen aus der Erfindung sowohl für die Augen
diagnostik wie auch für die Linsenproduktion, hängt doch die
Verbesserung des Materials und der Form der jetzt verwende
ten Linsenimplantate entscheidend davon ab, daß zunächst
deren pathogene Wirkung naturwissenschaftlich zureichend
nachzuweisen ist. Prima vista mag die Erfindung weniger
bedeutend erscheinen, als sie
a) wissenschaftlich und
b) wirtschaftlich tatsächlich ist.Advantages arise from the invention both for eye diagnosis and for lens production, but the improvement of the material and the shape of the lens implants now used depends crucially on the fact that their pathogenic effect is first scientifically proven. Prima vista, the invention may seem less important than it
a) scientifically and
b) is economically actual.
Zu a) Die Zahl der Linsenimplantationen ist weltweit sehr hoch (Staroperationen etc.). Diese chirurgischen Eingriffe haben leider gelegentlich zelluläre Reaktionen zur Folge, die den Implantationserfolg gefährden, weil die Ur sachen noch nicht eindeutig geklärt sind. Die Erfindung macht den exakten Ursachennachweis möglich.To a) The number of lens implantations worldwide is very high high (star operations etc.). This surgical Unfortunately, interventions occasionally lead to cellular reactions Consequence that endanger the success of the implantation because the original things have not yet been clearly clarified. The invention makes the exact cause evidence possible.
Zu b) Damit ist auch der technologische Aspekt der Erfin dung ersichtlich, also die Bedeutung für die For schung und Produktion zum Material und zur Formgebung der Kunstlinsen, die von vielen Institutionen und Firmen im In- und Ausland betrieben wird.To b) This is also the technological aspect of the inven can be seen, i.e. the meaning for the For research and production on the material and shape of the Artificial lenses used by many institutions and companies in and abroad.
Auf die erzielbaren Vorteile ist auch die hier zugrundege legte Bewertung der Erfindung zu beziehen, die mit 100 000,- DM jedenfalls nicht zu hoch angesetzt erscheint. Weitere Parameter für die Wertberechnung können unsererseits nicht beigebracht werden.The achievable advantages are also the basis here submitted to refer to the invention with 100 000, - DM does not appear to be set too high in any case. Further We cannot use parameters for the value calculation be taught.
Der Patentanmeldung sind hier vier Farbphotographien und Zeichnungen beigefügt, die den Präparationsablauf illustrieren.The patent application here are four color photographs and Drawings attached showing the preparation process illustrate.
Claims (1)
Verfahren zum Färben von Mikrotomschnitten von Bulbi mit chirurgisch implantierter Kunstlinse, das diese nicht zer stört.
Kennzeichnender Teil:
Die Färbemethode ist dadurch gekennzeichnet, daß sie das Implantat nicht auflöst, sondern erhält, so daß die Berüh rungsstellen von Kunstlinse und entzündeten Gewebeteilen in ihrer räumlichen Zuordnung mikroskopisch genau nachweisbar sind.Generic term
Process for staining microtome sections of bulbi with a surgically implanted artificial lens that does not destroy them.
Characteristic part:
The staining method is characterized in that it does not dissolve the implant, but receives it, so that the contact points of artificial lens and inflamed tissue parts can be detected microscopically in their spatial assignment.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7906579A FR2451386A1 (en) | 1979-03-06 | 1979-03-15 | Flame resistant thermoplastic compsns. - based on polyphenylene ether resin contg. di: and polyfunctional phosphate(s) |
| DE3909442A DE3909442A1 (en) | 1989-03-22 | 1989-03-22 | Microtome cutting method for positive detection of cellular reactions - using intra=ocular lens implants which do not dissolve but which are preserved, enabling position of connection region to be detected |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3909442A DE3909442A1 (en) | 1989-03-22 | 1989-03-22 | Microtome cutting method for positive detection of cellular reactions - using intra=ocular lens implants which do not dissolve but which are preserved, enabling position of connection region to be detected |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3909442A1 true DE3909442A1 (en) | 1991-05-16 |
| DE3909442C2 DE3909442C2 (en) | 1993-02-18 |
Family
ID=6376972
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE3909442A Granted DE3909442A1 (en) | 1979-03-06 | 1989-03-22 | Microtome cutting method for positive detection of cellular reactions - using intra=ocular lens implants which do not dissolve but which are preserved, enabling position of connection region to be detected |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3909442A1 (en) |
-
1989
- 1989-03-22 DE DE3909442A patent/DE3909442A1/en active Granted
Non-Patent Citations (2)
| Title |
|---|
| DE-B: ROMEIS, Benno * |
| Mikroskopische Technik, 16. Auflage, 1968, R. Oldenbourg, München und Wien, S. 63-104, 120-128,174,175,276 * |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3909442C2 (en) | 1993-02-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee | ||
| 8370 | Indication related to discontinuation of the patent is to be deleted | ||
| 8339 | Ceased/non-payment of the annual fee |