DE3878915D1 - METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA. - Google Patents

METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA.

Info

Publication number
DE3878915D1
DE3878915D1 DE8888907038T DE3878915T DE3878915D1 DE 3878915 D1 DE3878915 D1 DE 3878915D1 DE 8888907038 T DE8888907038 T DE 8888907038T DE 3878915 T DE3878915 T DE 3878915T DE 3878915 D1 DE3878915 D1 DE 3878915D1
Authority
DE
Germany
Prior art keywords
soln
filter
pptn
contg
extract
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8888907038T
Other languages
German (de)
Inventor
Wilhelm Ansorge
Tom Kristensen
Hartmut Voss
Josef Stegemann
Christian Schwager
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Europaisches Laboratorium fuer Molekularbiologie EMBL
Original Assignee
Europaisches Laboratorium fuer Molekularbiologie EMBL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europaisches Laboratorium fuer Molekularbiologie EMBL filed Critical Europaisches Laboratorium fuer Molekularbiologie EMBL
Priority to DE8888907038T priority Critical patent/DE3878915D1/en
Application granted granted Critical
Publication of DE3878915D1 publication Critical patent/DE3878915D1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Telephonic Communication Services (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Recovery or pure recombinant M-13-ss DNA (I) comprises (1) pptn. of recombinant M13 phages, excreted into the medium from an E. coli strain, by treatment with acetic acid; (2) filtering the resulting suspension through a glass fibre filter; then (3) treating the filter with a soln. contg. chastropic ions to extract the envelope proteins. Also new is an appts. for this process. Specifically, for pptn., acetic acid is added to a final concn. of 1-10% and the extract soln. is 4-8m perchlorate of pH 6.5-8.5. After extraction, the filter is washed with aq. EtOH and the purified DNA eluted with an aq. soln. of 0.2-2mM Tris-HCl (H 7-8) contg. 0.05-0.2mM EDTA.
DE8888907038T 1987-07-23 1988-07-21 METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA. Expired - Fee Related DE3878915D1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE8888907038T DE3878915D1 (en) 1987-07-23 1988-07-21 METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA.

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19873724442 DE3724442A1 (en) 1987-07-23 1987-07-23 METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA
PCT/EP1988/000659 WO1989001035A1 (en) 1987-07-23 1988-07-21 Process and device for purification of m-13 phage dna
DE8888907038T DE3878915D1 (en) 1987-07-23 1988-07-21 METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA.

Publications (1)

Publication Number Publication Date
DE3878915D1 true DE3878915D1 (en) 1993-04-08

Family

ID=6332230

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19873724442 Withdrawn DE3724442A1 (en) 1987-07-23 1987-07-23 METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA
DE8888907038T Expired - Fee Related DE3878915D1 (en) 1987-07-23 1988-07-21 METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE19873724442 Withdrawn DE3724442A1 (en) 1987-07-23 1987-07-23 METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA

Country Status (4)

Country Link
EP (1) EP0371067B1 (en)
JP (1) JPH02504224A (en)
DE (2) DE3724442A1 (en)
WO (1) WO1989001035A1 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2978518B2 (en) * 1989-12-06 1999-11-15 東ソー株式会社 Method for purifying phage DNA
CA2067711C (en) * 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
MX9202037A (en) * 1991-05-03 1992-11-01 Becton Dickinson Co PURIFICATION BY DNA FILTRATION.
WO1995034569A1 (en) * 1994-06-14 1995-12-21 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
KR100463475B1 (en) * 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 Magnetic Pigment
DE19520398B4 (en) * 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetic pigment
US5783686A (en) * 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
JPH09109083A (en) * 1995-10-19 1997-04-28 Agency Of Ind Science & Technol Linear motion fixed type parallel manipulator
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
WO2001014541A1 (en) * 1999-08-26 2001-03-01 Stroem Terje A method for preparation of dna from biological material
DE60025529T2 (en) 1999-11-17 2006-08-24 Roche Diagnostics Gmbh MAGNETIC GLASS PARTICLES, MANUFACTURING METHOD AND USE
US9394332B2 (en) 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
CA2614069C (en) 2006-12-11 2016-05-03 F. Hoffmann-La Roche Ag Nucleic acid isolation using polidocanol and derivatives
EP2126074B1 (en) 2006-12-13 2010-12-01 Roche Diagnostics GmbH Use of acetals for the isolation of nucleic acids
ES2394815T3 (en) 2006-12-13 2013-02-05 F. Hoffmann-La Roche Ag Use of TDE for nucleic acid isolation
CA2629586C (en) 2007-04-20 2016-05-24 F.Hoffmann-La Roche Ag Adsorption of nucleic acids to solid phases under low-salt conditions
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
EP2108699B1 (en) 2008-04-08 2014-06-25 F.Hoffmann-La Roche Ag Analytical processing and detection device
DE102008055606A1 (en) 2008-11-03 2010-05-06 Bayer Technology Services Gmbh A method for the high-throughput preparation of sequencing-competent DNA from individual plaques of peptides presenting phages

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8311905D0 (en) * 1983-04-29 1983-06-02 Cox R A Isolation and detection of nucleotide sequence
SE8402861L (en) * 1984-05-28 1985-11-29 Stefan Svenson PURIFICATION OF BIOLOGICAL MATERIAL
GB8523801D0 (en) * 1985-09-26 1985-10-30 Jones K W Vacuum molecular transfer(blotting)apparatus

Also Published As

Publication number Publication date
DE3724442A1 (en) 1989-02-02
EP0371067B1 (en) 1993-03-03
EP0371067A1 (en) 1990-06-06
JPH02504224A (en) 1990-12-06
WO1989001035A1 (en) 1989-02-09

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee