DE3818654A1 - Selective, laser-induced inactivation of proteins by fluorochrome labelling - Google Patents

Selective, laser-induced inactivation of proteins by fluorochrome labelling

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Publication number
DE3818654A1
DE3818654A1 DE3818654A DE3818654A DE3818654A1 DE 3818654 A1 DE3818654 A1 DE 3818654A1 DE 3818654 A DE3818654 A DE 3818654A DE 3818654 A DE3818654 A DE 3818654A DE 3818654 A1 DE3818654 A1 DE 3818654A1
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Germany
Prior art keywords
laser
proteins
induced inactivation
selective
macromolecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
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DE3818654A
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German (de)
Inventor
Benedikt Dr Med Jean
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Individual
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Individual
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Publication date
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Priority to DE3818654A priority Critical patent/DE3818654A1/en
Publication of DE3818654A1 publication Critical patent/DE3818654A1/en
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
    • A61K41/10—Inactivation or decontamination of a medicinal preparation prior to administration to an animal or a person
    • A61K41/17—Inactivation or decontamination of a medicinal preparation prior to administration to an animal or a person by ultraviolet [UV] or infrared [IR] light, X-rays or gamma rays

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for the laser-induced inactivation of macromolecules and proteins is made selective by the proposal of the invention. This takes place in such a way that certain macromolecules are labelled by covalent coupling to dye molecules (chromophores) so that in the subsequent laser irradiation, owing to the high absorption of the chromophores, the energy transfer to the macromolecule is so high that its tertiary structure changes and there is a corresponding reduction or loss of activity.

Description

Die Erfindung betrifft ein Verfahren zur laserinduzierten Inaktivierung von Makromolekülen und Proteinen, wie z. B. Enzyme und krankheitserzeugende Antikörper, zur Verwendung in biochemischen, chemischen oder physikalisch-chemischen Prozessen.The invention relates to a method for laser-induced Inactivation of macromolecules and proteins, such as B. Enzymes and disease-producing antibodies, for use in biochemical, chemical or physico-chemical Processes.

Der Effekt der lichtinduzierten Inaktivierung ist in der Grundlagenforschung unter dem Stichwort Mikrofotolyse oder Fotobleeching bekannt. Er wurde bisher genutzt, um Eigenschaften von Membranen in Vitro untersuchen zu können.The effect of light-induced inactivation is in the Basic research under the keyword microfotolysis or Photo bleeding known. It has been used to date Investigate properties of membranes in vitro can.

Die gängigen Behandlungsverfahren im Bereich der Medizin basieren auf einer teilweisen oder völligen Unterbindung der Antikörperproduktion (z. B. mit Nebennierenrinden- Hormonen oder Immunsuppressiva). Die Nebenwirkungen sind erheblich, der Effekt zweifelhaft.The common treatment methods in the field of medicine are based on a partial or total prohibition antibody production (e.g. with adrenal cortex Hormones or immunosuppressants). The side effects are considerable, the effect doubtful.

Durch vollkommenen Austausch aller Immunproteine in der Plasmaphorese werden zwar alle krankhaft veränderten, daneben aber auch alle nicht krankheitsrelevanten Immunproteine entfernt, so daß innerhalb dieses Behandlungsverfahrens eine Neuzufuhr von Fremdeiweiß zur Erhaltung der Körperabwehr erforderlich ist. Through the complete exchange of all immune proteins in the Plasma phoresis are all morbidly changed, but also all non-illness-related Immune proteins removed so that within this Treatment procedure a new supply of foreign protein for Maintaining body defense is required.  

Der Erfindung liegt die Aufgabe zugrunde, krankheitserzeugende Makromoleküle, die in der Pathogenese rheumatischer Erkrankungen oder immunvermittelter chronischer Entzündungen (z. B. am Auge) eine Rolle spielen, selektiv zu eliminieren.The invention is based on the object disease-producing macromolecules that are in pathogenesis rheumatic diseases or immune mediated chronic inflammation (e.g. on the eye) plays a role play to selectively eliminate.

Die Lösung besteht in der selektiven Markierung mit Fluorochromen und anschließender Laserbestrahlung. Dadurch konnen einzelne Proteinpopulationen gezielt angegangen werden. Sind die Makromoleküle nämlich mit Fluorochrom markiert, so können sie einer gezielten Vernichtung und zahlenmäßigen Reduktion in einem Reaktionsmedium, einer Körperflüssigkeit o. ä., zugänglich gemacht werden.The solution is selective labeling with fluorochromes and subsequent laser irradiation. This means that individual protein populations can be targeted. If the macromolecules are labeled with fluorochrome, they can be deliberately destroyed and reduced in number in a reaction medium, a body fluid or the like . Ä., be made accessible.

Das Verfahren basiert auf den Gesetzen der linearen Optik. Die Strahlung eines Lasers, z. B. eines Farbstofflasers, entsprechender Emissionswellenlänge wird von dem mit dem Makromolekül gekoppelten Chromophor oder mehreren Chromophoren absorbiert. Durch Energietransfer wird die im Chromophor entstehende Wärme in das Makromolekül weitergeleitet, so daß es zu einer Veränderung seiner Tertiärstruktur (räumliche Konfiguration) kommt und dadurch eine Wirkungseinschränkung oder Wirkungsverlust eintritt.The process is based on the laws of linear optics. The radiation from a laser, e.g. B. a dye laser, corresponding emission wavelength is from that with the Macromolecule-linked chromophore or more Chromophores absorbed. Through energy transfer, the im Chromophore heat generated in the macromolecule forwarded so that there is a change in its Tertiary structure (spatial configuration) comes and thereby an impact restriction or loss of effectiveness entry.

Claims (2)

1. Verfahren zur laserinduzierten Inaktivierung von Makromolekülen, dadurch gekennzeichnet, daß bestimmte Makromoleküle eines Reaktionsmediums kovalent mit Farbstoffmolekülen (Chromophoren) gekoppelt werden und sodann das Reaktionsmedium einer Laserstrahlung unterworfen wird.1. A method for laser-induced inactivation of macromolecules, characterized in that certain macromolecules of a reaction medium are covalently coupled with dye molecules (chromophores) and then the reaction medium is subjected to laser radiation. 2. Verfahren nach Anspruch 1, dadurch gekennzeichnet, daß die Chromophore nach ihrem maximalen Absorptionsverhalten ausgewählt werden in Abstimmung auf die Emissionswellenlänge des verwendeten Lasers.2. The method according to claim 1, characterized in that that the chromophores are at their maximum Absorption behavior can be selected in coordination the emission wavelength of the laser used.
DE3818654A 1988-06-01 1988-06-01 Selective, laser-induced inactivation of proteins by fluorochrome labelling Withdrawn DE3818654A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE3818654A DE3818654A1 (en) 1988-06-01 1988-06-01 Selective, laser-induced inactivation of proteins by fluorochrome labelling

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3818654A DE3818654A1 (en) 1988-06-01 1988-06-01 Selective, laser-induced inactivation of proteins by fluorochrome labelling

Publications (1)

Publication Number Publication Date
DE3818654A1 true DE3818654A1 (en) 1989-12-14

Family

ID=6355622

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3818654A Withdrawn DE3818654A1 (en) 1988-06-01 1988-06-01 Selective, laser-induced inactivation of proteins by fluorochrome labelling

Country Status (1)

Country Link
DE (1) DE3818654A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000031517A1 (en) * 1998-11-24 2000-06-02 Xerion Pharmaceuticals Gmbh Method and device for automated chromophore-assisted laser/light inactivation (cali)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000031517A1 (en) * 1998-11-24 2000-06-02 Xerion Pharmaceuticals Gmbh Method and device for automated chromophore-assisted laser/light inactivation (cali)

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