DE3778792D1 - New gene for resistance to phosphinothricine - Google Patents

New gene for resistance to phosphinothricine

Info

Publication number
DE3778792D1
DE3778792D1 DE87112023T DE3778792T DE3778792D1 DE 3778792 D1 DE3778792 D1 DE 3778792D1 DE 87112023 T DE87112023 T DE 87112023T DE 3778792 T DE3778792 T DE 3778792T DE 3778792 D1 DE3778792 D1 DE 3778792D1
Authority
DE
Germany
Prior art keywords
resistance
fragment
ptt
isolated
ptc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE87112023T
Other languages
German (de)
Inventor
Eckhard Strauch
Dr. Wolfgang Wohlleben
Walter Arnold
Renate Alijah
Prof. Dr. Alfred W-4800 Bielefeld De Puehler
Dr. Gerhard W-6093 Floersheim Am Main De Woehner
Dr. Ruediger W-6000 Frankfurt Am Main De Marquardt
Dr. Susanne W-6240 Koenigstein/Taunus De Grabley
Dr. Dieter W-6093 Floersheim Am Main De Brauer
Dr. Klaus W-6233 Kelkheim Bartsch (Taunus), De
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Priority to DE87112023T priority Critical patent/DE3778792D1/en
Application granted granted Critical
Publication of DE3778792D1 publication Critical patent/DE3778792D1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C12P41/007—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures by reactions involving acyl derivatives of racemic amines

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The gene for resistance to phosphinothricin (PTC; 2-amino-4-methylphosphino-butyric acid) is new. It is derived from the total DNA of Streptomyces viridochromogenes DSM 4112 (selected for resistance to phosphothricylalanyl-alamino, PTT) by cutting with BamHI, cloning the resulting Ak6 fragment and selection for PTT resistance. DNA was isolated from colonies of DSM 4112 resistant to 100 microg/ml PTT, cut with BamHI and fragments from pSVH1 by ligation with a thiostreptone-resistance fragment described in EP 158201). Recombinants were used to transform S.lividans TK23 and PTT-resistant colonies isolated. These colonies contained the plasmid pPRI. The 4kb fragment in pRI was cut first with SstII-SstI and then with kgl II to isolated a 0.8 kb fragment able to impart resistance to S.lividans. The DNA sequence of this 0.8 kb fragment, and of possible amino acid sequences derived from it, and reproduced in the specification. The gene codes for a PTC-octeyltransferase which selectively acylates the L-form of PTC in a racemate. This enzyme can be isolated from cultures but more conviently is used in the form of cells.
DE87112023T 1986-08-23 1987-08-19 New gene for resistance to phosphinothricine Expired - Lifetime DE3778792D1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE87112023T DE3778792D1 (en) 1986-08-23 1987-08-19 New gene for resistance to phosphinothricine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19863628747 DE3628747A1 (en) 1986-08-23 1986-08-23 Phosphinothricin resistance gene and its use
DE87112023T DE3778792D1 (en) 1986-08-23 1987-08-19 New gene for resistance to phosphinothricine

Publications (1)

Publication Number Publication Date
DE3778792D1 true DE3778792D1 (en) 1992-06-11

Family

ID=6308093

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19863628747 Withdrawn DE3628747A1 (en) 1986-08-23 1986-08-23 Phosphinothricin resistance gene and its use
DE87112023T Expired - Lifetime DE3778792D1 (en) 1986-08-23 1987-08-19 New gene for resistance to phosphinothricine

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE19863628747 Withdrawn DE3628747A1 (en) 1986-08-23 1986-08-23 Phosphinothricin resistance gene and its use

Country Status (2)

Country Link
DE (2) DE3628747A1 (en)
ZA (1) ZA876210B (en)

Also Published As

Publication number Publication date
ZA876210B (en) 1988-02-23
DE3628747A1 (en) 1988-02-25

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: HOECHST SCHERING AGREVO GMBH, 13509 BERLIN, DE

8327 Change in the person/name/address of the patent owner

Owner name: AVENTIS CROPSCIENCE GMBH, 13509 BERLIN, DE

8327 Change in the person/name/address of the patent owner

Owner name: AVENTIS CROPSCIENCE GMBH, 65929 FRANKFURT, DE

8327 Change in the person/name/address of the patent owner

Owner name: BAYER CROPSCIENCE GMBH, 65929 FRANKFURT, DE

8327 Change in the person/name/address of the patent owner

Owner name: BAYER CROPSCIENCE AG, 40789 MONHEIM, DE