DE3608175A1 - EFOMYCIN G, ITS PRODUCTION AND USE AS A PERFORMANCE IN ANIMALS - Google Patents

Description

The present invention relates to efomycin G, its Manufacturing and its use as a performance enhancer Animals.

• 1. Efomycin G with the following chemical and physical properties was found:
  • 1. The empirical formula: C ₅₃ H ₈₆ O ₁₈
  • 2. The mass spectrum (Fast Atom Bombardment) molar mass + Na⁺: 1033
  • 3. The ¹ H nuclear magnetic resonance spectrum, given in parts per million, according to FIG. 1.
    It was recorded in an AM 300 from Bruker at MHz on a solution of efomycin G in deuterated chloroform with tetramethylsilane as the internal standard.
  • 4. The ¹³ C nuclear magnetic resonance spectrum, given in parts per million, according to FIG. 2.
    It was recorded in an AM 300 from Bruker at 75.48 MHz on a solution of efomycin G in deuterated chloroform and deuterated methanol with tetramethylsilane as the internal standard.
• The chemical shifts of the ¹³ C-NMR signals of efomycin G according to Fig. 2 are as follows:
  • 5. The UV absorption maximum at 251-254 nm in methanolic solution.
• 2. It has been found that the efomycin G according to the invention is obtained if suitable microorganisms from the Streptomycetaceae family are cultivated under aerobic conditions in a nutrient medium which contains assimilable carbon and nitrogen sources and mineral salts, and the resulting mixture of efomycin is isolated and separated by customary methods .
  If the properties of efomycin G are known, it is possible to identify suitable microorganism strains that produce efomycin G using the usual chromatographic, spectroscopic and / or biological detection methods.
  Streptomyces strain BS 1261 - and its mutants and variants - can be used in particular to carry out the method.
  This strain belongs to the Streptomycetaceae family, the genus Streptomyces from the gray series of Streptomycetes (Cinereus group).
  The strain BS 1261 was deposited on January 16, 1985 with the German Collection for Microorganisms (DSM) Grisebachstrasse 8, 3400 Göttingen, Federal Republic of Germany under the number DSM 3200.

Taxonomic description of the strain BS 1261 (DSM 3200)

The taxonomic description of the strain BS 1261 was done according to Bergey's Manual of Determinative Bacteriology 8th, (1974) and International Journal of Systematic Bacteriology 16, 313-340 (1966) and The Prokaryotes 2, 2028-2020 (1981).

1. Morphology

Good sporulation was on ISP media # 2, # 3, 4, 5, 7 to 9 can be observed. On ISP medium No. 9 with Ribose as a C source and from ISP medium No. 1 and 6 substrate mycelium was predominantly formed.

Air mycelium (ISP medium No. 3, 28 ° C, 7 days:

Color: gray (Cinereus type) spore chains: retinaculum aperture type spores: square to rectangular,
1.4-1.8 σ m long and 1.3-1.6 µm wide
smooth (electron microscopy).

Substrate mycelium:

Color: brown  

2. Information on physiology

The temperature optimum is 28 ° C (on ISP medium No. 2.5 days). The trunk does not grow at 4 ° and 45 ° C. Melanin is not produced. The growth is inhibited by the antibiotics erythromycin (10 σ g), sulphafurazole (100 µg), streptomycin (10 σ g) and novobiocin (5 µg) (ISP medium No. 2, 28 ° C, 2 days).

The utilization of C sources was carried out on basal agar (ISP medium No. 9) according to Int. Syst. Bact. 16, 313-340 (1966). For the negative control, the growth on basal agar without a C source was compared. The following results are obtained:
C source (10 g / l) growth *)

Control (no C source) - D-glucose + L-arabinose + L-rhamnose + D-fructose + L-galactose + Raffinose + D-Mannitol + meso-inositol + Salicin + Sucrose + Ribose + Mannose + Maltose + Mellibiosis + Cellulose Acetate-

*) + = Growth, - = no growth.  

3. Particularly suitable medium for growth and Sporulation ISP 3 (oatmeal agar)

20 g of oatmeal are in 1000 ml of H ₂ O deion. suspended and boiled for 20 minutes. It is then filtered, 1 ml of trace element solution for ISP 3 and 18 g of agar are added, the pH is adjusted to 7.2 and autoclaved at 121 ° C. for 15-20 minutes.

Trace element solution for ISP 3

FeSO ₄ · 7 H ₂ O 0.1 g MnCl ₂ · 4 H ₂ O 0.1 g ZnSO ₄ · 7 H ₂ O 0.1 g H ₂ O deion. 100 ml

Further media see Int. J. Syst. Bact. 16, 313-340 (1966).

The strain BS 1261 isolated from an earth sample New Zealand, based on the morphological data in the gray series of Streptomycetes (Cinereus group) classify.  

Taxonomic name: Streptomyces sp.

According to the invention, efomycin G is produced by fermentation suitable microorganisms, such as the Streptomycetes strain BS 1261 or its mutants or variants generated.

The fermentation process according to the invention can with Help with solid, semi-solid or liquid nutrient media be performed. Aqueous-liquid are preferred Culture media used.

The inoculation of the nutrient media is carried out generally usual methods, e.g. B. over inclined tubes or Piston cultures.

The culture takes place under aerobic conditions and can according to the commonly used methods such as using of shake cultures or of submerged cultures be performed. Cultivation is preferred in the aerobic submerged process in aerated fermenters, e.g. B. in conventional submerged fermentation tanks. It is possible, the fermentation continuously or discontinuously perform. It is preferably discontinuous worked.

The culture is carried out in nutrient media, which known for the cultivation of microorganisms Order Actinomycetales can be used. The nutrient medium  must have one or more assimilable carbon sources and contain nitrogen sources and mineral salts, these products being in the form of defined Individual components, but also in the form of complex ones Mixtures, such as those of biological products represent different origins. All common carbon sources come as carbon sources in question. For example, carbohydrates in particular polysaccharides, such as starch or dextrins, Disaccharides, such as maltose or lactose, monosaccharides, such as glucose or xylose, alcohols such as mannitol or Glycerin as well as naturally occurring mixtures, such as Malt extract, molasses or whey powder called. As Nitrogen sources come in all the usual organic and inorganic Nitrogen sources in question. For example be protein substances, protein hydrolyzates, amino acids, Nucleoside bases such as cytosine or uracil as well Soybean meal, cottonseed meal, lentil meal, pea meal, soluble and insoluble vegetable proteins, corn steep liquor, Yeast extract, peptones and meat extract, nitrogen-containing salts such as B. ammonium salts and Nitrates. The mineral salts, which in the Nutrient medium should be included, z. B. following Ions:

Mg ⁺⁺ , Na⁺, K⁺, Ca ⁺⁺ , NH ₄ ⁺,
Cl ^- , SO ₄ ^- , PO ₄ ^--- and NO ₃ ^-

and ions of the usual trace elements, such as Cu, Fe, Mn, Mo, Zn, CO, Ni. If the carbon or nitrogen sources or the water used is insufficient  contain these salts or trace elements, it is advisable supplement the nutrient medium accordingly. The Composition of the nutrient media can vary widely can be varied. Type and composition of the nutrient media will generally depend on which Components are available particularly cheaply. In general, the nutrient solutions preferably contain about 0.5 to 8%, in particular 0.6 to 6% Carbon sources, preferably about 0.5 to 4%, especially 0.5 to 2% nitrogen sources and preferably about 0.001 to 0.5%, especially 0.003 to 0.3% mineral salts.

When performing the procedure it can be convenient be relatively small at the beginning of cultivation Concentrations of soluble nutrient components to use and then during the first 3 days of cultivation through more frequent additions to these ingredients in the form of sterile, relatively concentrated solutions Feed the culture approach fractionally.

The pH of the growing cultures should preferably be kept between about 5 and about 10, in particular between 6.5 and 8.0. An excessive drop in pH in the acidic areas can be avoided by adding an organic or inorganic base, preferably CaCO ₃ . As usual in fermentation technology, an automatic pH control can also be carried out in which sterile organic or inorganic acids, e.g. B. H ₂ SO ₄ , or sterile bases, e.g. B. NaOH are injected into the culture solution at intervals.

It is advisable to ensure that the microorganisms sufficient with oxygen and the nutrients in Be brought in contact. This can be done according to the general usual methods such as shaking and stirring.

The cultivation temperature can be between about 24 ° C and about 34 ° C, preferably between 26 ° C and 32 ° C, it is particularly preferably about 28 ° C. The duration the breeding can be varied widely, z. B. the Composition of the nutrient medium and the breeding temperature play a role. The respective optimal Conditions can be determined by any specialist on the microbiological area can be easily set.

It has been found that the amount of in the culture broth enriching the invention Compound generally their maximum about 1 to 10, preferably about 4 to 7 days after the start of breeding reached. The desired end product of the fermentation can be done using thin layer chromatography and high pressure liquid chromatographic investigations or biological test methods can be determined.

As is common in microbiological processes foreign infections of the culture media should be avoided will. To do this, take the usual precautions taken, such as sterilization of the culture media, culture vessels  as well as the air required for ventilation. To Sterilization of the devices can e.g. B. the steam as well as dry sterilization can be used the temperatures preferably at 100 to 140 ° C, can be in particular at 120 to 130 ° C.

If foam in the undesirable amount during the cultivation arises, the usual chemical Anti-foaming agents, e.g. B. liquid fats and oils, such as oil Water emulsions, paraffins, higher alcohols, such as Octadecanol, silicone oils, polyoxyethylene or Polyoxypropylene compounds (e.g. in amounts up to about 1%) be added. Foam can also be made using the usual mechanical devices (e.g. centrifugal forces use) are dampened or eliminated.

The compound according to the invention can be obtained from the culture medium according to common physical-chemical Methods are isolated. The insulation can e.g. B. according the usual extraction processes, precipitation processes and / or chromatography processes. The Isolated substances can be made using the methods mentioned can also be cleaned. For many cases, however a fine cleaning is not necessary, because if necessary existing minor impurities the effectiveness not adversely affect the connections. At all Isolation and cleaning operations are approaching make sure that the pH values are in the neutral range. PH values between 7 and 8 are preferably maintained. Inorganic ones can be used to adjust the pH  and organic bases are used, such as Alkali bases e.g. B. NaOH, KOH or organic amines, such as Triethylamine; inorganic acids such as B. HCl and organic acids such as B. acetic acid.

In order to find out the fractions in the above-mentioned isolation and cleaning methods in which the compound according to the invention is present in the highest concentration or purity, the usual physico-chemical methods can be used, for. B. measurement of a characteristic band in the spectrum or the R _f values, determination of the antibacterial activity, etc. are used. These methods can also be used to find suitable microorganisms in routine procedures for the production of efomycin G.

The isolation and cleaning of the invention Connection can e.g. B. in the event that a liquid aqueous Culture medium is used as follows will:

Since efomycin G is present in both the culture supernatant and the To find mycelium, it can be done with the help of usual Extraction process, precipitation process and / or Chromatography process isolated from the fermentation batch and cleaned if necessary. Chromatography can be carried out in the form of column chromatography will. With good success, too Use high pressure liquid chromatography (HPLC). As Adsorbents can be the usual inorganic or  organic adsorbents are used, such as e.g. B. silica gel, magnesium silicate, activated carbon, cellulose, Cellulose derivatives, synthetic resins such as polyamides, e.g. B. acetylated polyamide, dextran gels or modified Dextran gels. A wide variety of solvents can be used Solvent or solvent mixture used in which the compounds of the invention are soluble. Preferred are ethyl acetate, Chloroform and methanol or their mixtures (e.g. mixtures of chloroform and methanol or from ethyl acetate and chloroform) used.

Are preferred for the isolation of the invention Compounds chromatography method used, e.g. B. non-specific adsorption on sorbents such as silica gel or on the other hand gel diffusion chromatography. This are less water-soluble from cleaning Natural products known methods.

The solutions according to the invention can be obtained from their solutions Connection by the usual methods, e.g. B. Evaporation of the solvent, freeze drying, etc. obtained will.

In a preferred embodiment, the mycelium is separated from the culture broth, preferably by centrifugation, and extracted several times, preferably twice, with a water-miscible solvent. (C ₁ -C ₄ ) -Alkyl alcohols, C ₁ - ₄ ketones, particularly preferably acetone, can be used as solvents. The aqueous-organic solution is in vacuo, for. B. concentrated to about ½₀ of the volume of the culture broth and freeze-dried.

This raw preparation is suspended in water and the Efomycin G with a water-immiscible Solvents such as B. chlorinated hydrocarbons such as Chloroform, esters of acetic acid or ketones extracted. From this extract, efomycin G can be made through usual chromatographic methods, preferably chromatography on silica gel.

Efomycin G can also pass through from the culture filtrate Extraction with a water-immiscible Solvents such as ethyl acetate, methylene chloride or Chloroform can be extracted.

It can also affect unspecific adsorber resins Polystyrene base (e.g. Amberlite XAD from the company Roehm u. Haas or Lewatit OC 1031 from Bayer) be bound. The desorption is fractionated by Mixtures of water and organic solvents, especially water / methanol. The through test active against Staphylococcus aureus 1756 Fractions are made under reduced pressure up to complete removal of the organic solvent 30-35 ° C concentrated and the residue in about ¹ / ₁₀₀ of the volume of the culture filtrate suspended and freeze-dried.  

The lyophilisate is resuspended in water and preferably not with ethyl acetate or others extracted with water-miscible solvents. From the Efomycin G is extracted by conventional chromatographic Methods, preferably chromatography on silica gel, won.

The connection according to the invention shows a good one antibacterial effect especially against gram-positive germs. Their suitability for prevention should be mentioned in particular and healing of swine dysentery.

The active ingredient is used as a performance enhancer in animals to promote and accelerate the growth of Milk and wool production, and to improve Feed utilization, meat quality and Shift in meat-fat ratio in favor of Meat used. The active ingredient is used in Breeding, ornamental and hobby animals used.

The livestock and breeding animals include mammals such as B. Cattle, pigs, horses, sheep, goats, rabbits, Rabbits, fallow deer, fur animals such as mink, chinchilla, poultry such as B. chickens, geese, ducks, turkeys, pigeons, fish like e.g. B. carp, trout, salmon, eels, tench, pike, Reptiles such as B. snakes and crocodiles.  

Ornamental and hobby animals include acidic animals such as dogs and cats, birds like parrots, canaries, fish like Ornamental and aquarium fish e.g. B. Goldfish.

The active ingredient is independent of the sex of the animals used during all performance phases of the animals. The active ingredient is preferred during the intensive Service phase used. The intense The performance phase lasts one month depending on the animal species up to 10 years.

The amount of active ingredient that the animals achieve the desired effect is administered, because of the favorable properties of the active ingredient largely varied will. It is preferably about 0.001 to 500 mg / kg in particular 0.01 to 5 mg / kg body weight per Day. The right amount of the active ingredient and the right one Duration of administration depend in particular on the type the age, the gender, the performance phase, the State of health and the way of keeping and feeding the Animals from and are easily closed by any specialist determine.

The active ingredient is used in animals according to the usual methods administered. The mode of administration depends in particular on the nature, behavior and health of the Animals off.

The active substance can be administered once. The Active ingredient can also be used throughout or during part of the service phase temporarily or continuously be administered.  

With continuous administration, the application can or several times a day in regular or irregular Intervals.

It is administered orally in suitable formulations or in pure form.

The active ingredient can be used alone or in the formulations in a mixture with other performance-enhancing active ingredients, Vitamins, non-protein compounds, dyes, Antioxidants, flavors, emulsifiers, flow aids, Preservatives and pressing aids are available.

Other performance-enhancing active ingredients are: e.g. B. antibiotics such as tylosin, virginiamycin and Monensin. Mineral feeds are e.g. B. Dicalcium phosphate, magnesium oxide, sodium chloride.

Trace element compounds are e.g. B. iron fumarate, sodium iodide, cobalt chloride, copper sulfate, zinc oxide.
Vitamins are e.g. B. Vitamin A, Vitamin D ₃ , Vitamin E, B Vitamins, Vitamin C.
Non-protein compounds are e.g. B. biuret, urea,
Dyes are e.g. B. carotenoids such as citranaxanthin, zeaxanthin, capsanthin.
Antioxidants are e.g. B. ethoxyquin, butylhydroxy-toluene.
Flavorings are e.g. B. Vanillin.
Emulsifiers are e.g. B. esters of lactic acid, lecithin,
Flow aids are e.g. B. sodium stearate, calcium stearate.
Preservatives are e.g. B. citric acid, propionic acid.
Pressing aids are e.g. B. lignin sulfonates, cellulose ethers.

The active ingredients can also be administered together with the feed and / or the drinking water.

Feed includes plant-based feed Origin such as hay, beets, grain by-products, feed materials of animal origin such as meat, fats, dairy products, Bone meal, fish products, feed materials such as vitamins, proteins, amino acid z. B. DL-methionine, Salts like lime and table salt. Food also counts Complementary, finished and compound feed. These contain Single feed in a composition one balanced nutrition with regard to energy and protein supply as well as the supply of vitamins, mineral salts and ensure trace elements.

The concentration of the active ingredient in the feed is normally about 0.01-500 ppm, preferably 0.1-50 ppm.

The active ingredient can be used as such or in the form of premixes or feed concentrates are added to the feed.

Example of the composition of a feed for cattle, which contains the active ingredient according to the invention:

69.95% feed grain meal, 10% ground corn on the cob, 8% soybean meal, 5% alfalfameh, 5% molasses, 0.6% urea, 0.5% calcium phosphate, 0.5% calcium carbonate, 0.3% table salt, 0.07 % Vitamin A and D ₂ premix, 0.05% vitamin E premix, and 0.03% trace element premix.

Active ingredient premix, the 1-800 g Contains active ingredient per kg added.

It is not absolutely necessary to have cleaned and to use isolated efomycin G. It is also possible, the mixture obtained in its manufacture or even the resulting culture broth or the mycelium without cleaning, if necessary after drying. For many purposes, it is also sufficient Raw forms of the active ingredient according to the invention and its Use mixtures without prior fine cleaning.

The production and biological effects of the new The compound of the invention can be obtained by the following Examples are explained:

Example 1 Production of the inoculum

Streptomyces sp. BS 1261 (DSM 3200) from a slant tube in 1000 ml Erlenmeyer flasks transferred, each 150 ml of the following sterile nutrient solution contained:

CASO® from Merck, Darmstadt, the composition:

Peptone from casein 15 g Soy flour peptone 5 g Glucose 2.5 g NaCl5 g Water spout 1000 ml

The flasks were at 250 Kpm at 28 ° C for 3 days incubating machine. 2 pistons of resulting culture were combined and served as Inoculum for a 30 l fermenter, the 20 l above, sterile nutrient solution, mixed with 20 ml SAG 5693 (Union Carbides).

The fermentation was at 28 ° C with an aeration rate of 10 l / min (0.5 VVM) air and a superimposition pressure of 0.5 bar. The speed of the blade stirrer was 300 rpm. After 48 hours, the so obtained served Culture as an inoculum for tank fermentation.  

Example 2 Tank fermentation

With 20 liters of the inoculum prepared according to Example 1 a 300 l kettle was inoculated, the 200 l sterile The nutrient medium contained the following composition:

Skimmed milk powder 10 g Yeast autolysate 1.5 g Dextrin 40 g D-glucose 5 g SAG 5693 (Union Carbide) 1 ml Water spout 1000 ml

The pH of the medium after sterilization was 6.6. The fermentation was at 28 ° C at a speed of Blade stirrer of 100 rpm, an aeration rate of 100 l / min (0.5 VVM) air and a superimposed pressure of 1.0 bar carried out until after 3-5 days efomycin G could be demonstrated.  

Example 3

The culture broth obtained according to Example 2 of a 200 liter Fermentation is carried out at pH 7-7.5 with 200-250 l / h separated by a Westfalia separator. The mycelium comes with double the volume of acetone for 30 minutes at room temperature stirred and centrifuged. The backlog will reappear stirred with twice the volume of acetone and centrifuged. The combined centrifugates are reduced Pressure concentrated at 40 ° C bath temperature. The The concentrate is mixed with 10 liters of water and three times extracted with 10 liters of dichloromethane each. The United organic phases were dried over sodium sulfate, filtered and constricted under reduced pressure at max. 40 ° bath temperature to 3 liters. The concentrate dropped one in about 30 liters of light petrol with stirring. The precipitate was filtered off and in vacuo dried at 40 ° C. Yield: 61 g of yellowish powder.

Example 4

Efomycin G was isolated from that obtained according to Example 3 Crude product through prepared liquid chromatography.

Parameter:

Superplasticizer A:
5 mM citric acid: acetonitrile = 6: 4
Superplasticizer B:
5 mM citric acid: acetonitrile = 4: 6
Gradient:
after 3 minutes of isocratic run with solvent A: B = 4: 1 linear gradient in 1 from A: B = 4: 1 to A: B = 1: 9

Flow rate: 20 ml / min Detection: UV 254 nm Column: 21.6 × 250 mm stationary phase: Zorbax® C8 8 µm
(DuPont)

Approx. 30 mg of that according to Example 3 obtained crude product dissolved in 2 ml each Tetrahydrofuran / water = 2/1 used. The Pillar Senate was collected fractionally and an analytical High pressure liquid chromatography according to the following Subject to parameters.

Solvent A: 5 mM citric acid
0.1 M CaClO ₄ eluent B: acetonitrile gradient: linear 52-75% B
in 18 minutes flow rate: 1.5 ml / min. Detection: UV 254 nm Stationary phase: Nucleosil® 10C18 column: 4.6 × 250 ml

Fractions containing pure efomycin G were made combined and under reduced pressure at 40 ° C bath temperature concentrated to ¹ / ₃ of the initial volume. That as Colorless precipitate resulting efomycin G was filtered off and dried in a high vacuum at 40 ° C. Yield: 5 mg efomycin G; Purity better than HPLC 85%.  

Example A:

A mutton that feeds 650 g of coarsely ground sheep Ready feed and 250 g of dry green cobs was obtained Rumen fluid removed through a rumen fistula. The The finished feed was fed through an automatic feeder in 12 equal portions every two hours and the Cobs in 2 equal portions at 8.30 a.m. and 4.15 p.m. administered. The rumen fluid became immediately after subject to the following treatment: 2.5 ml of the rumeninoculum were in one with carbon dioxide fumigated test tubes of 13 ml volume which also contained the following additions:

100 mg fine ground sheep's feed 7.5 ml buffer solution 0.5 ml solution with or without efomycin G

The composition of the buffer solution, which before starting of the trial saturated with carbon dioxide was like follows:

Na ₂ HPO ₄ 4.61 g per liter water NaHCO ₃ 12.25 g per liter water NaCl 0.59 g per liter water KCl 0.71 g per liter water MgCl ₂ 0.32 g per liter water CaCl ₂ 0.13 g per liter of water

0.5 ml of a solution which contained the desired amount of the compounds according to the invention in 5% strength aqueous ethanol was added to this mixture. The total volume of the fermentation mixture was then 10.5 ml. Each test tube was closed with a Bunsen stopper and incubated at 39 ° C. After 2, 4, 6 and 8 hours, the batches were shaken by hand. After incubation for 24 hours, 1.0 ml of the fermentation liquid was removed from the batches and pipetted into an Eppendorf tube containing 0.2 ml of 10% phosphoric acid (containing 5.7 σ mol 2-methylvaleric acid). The samples were centrifuged at 11,000 g and the volatile fatty acid concentrations were determined by gas chromatography from the supernatant.

The ratio of acetic acid was increased in each experiment Determined propionic acid. The one in the negative controls value obtained was set at 100 and the deviations stated in relation to it. The more propionic acid was formed, the lower the ratio Acetic acid to propionic acid and the smaller the Ratio compared to control (low Ratio = reduced acetic acid / propionic acid Ratio = improved feed conversion).

In addition, the concentrations in each experiment of total fatty acids compared to the control (= 100) specified.  

table

Claims (9)

1. Efomycin G, which is characterized by the following chemical and physical properties:

• 1. The empirical formula: C ₅₃ H ₈₆ O ₁₈
• 2. The mass spectrum (Fast Atom Bombardment) molar mass + Na⁺: 1033
• 3. The ¹ H nuclear magnetic resonance spectrum, given in parts per million, according to FIG. 1.
  It was recorded in an AM 300 from Bruker at MHz on a solution of efomycin G in deuterated chloroform with tetramethylsilane as the internal standard.
• 4. The 13 C nuclear magnetic resonance spectrum, given in parts per million, according to FIG. 2.
  It was recorded in an AM 300 from Bruker at 75.48 MHz on a solution of efomycin G in deuterated chloroform and deuterated methanol with tetramethylsilane as the internal standard.

The chemical shifts of the CC-NMR signals of efomycin G according to Fig. 2 are as follows: (ppm values are given relative to tetramethylsilane at 0 ppm)

• 5. The UV absorption maximum at 251-254 nm in methanolic solution.

2. Process for the preparation of efomycin G, characterized, that suitable microorganisms of the family of Streptomycetaceae in a nutrient medium Assimilable sources of carbon and nitrogen as well as contains mineral salts under aerobic Cultivated conditions, the resulting mixture of Efomycine isolated by usual methods and Efomycin G separates from it. 3. The method according to claim 2, characterized in that that it is with the strain BS 1261 (according to DSM No. 3200).   4. Use of efomycin G as a performance enhancer Animals. 5. means for promoting performance of animals, marked by containing efomycin G. 6. Animal feed, drinking water for animals, additives for Animal feed and drinking water for animals, labeled by containing efomycin G. 7. Process for the preparation of agents for Performance promotion of animals, characterized, that Efomycine G with stretch and / or Diluents mixed.