DE3577725D1 - Expressionsvektoren mit hoher kopienanzahl. - Google Patents
Expressionsvektoren mit hoher kopienanzahl.Info
- Publication number
- DE3577725D1 DE3577725D1 DE8585901488T DE3577725T DE3577725D1 DE 3577725 D1 DE3577725 D1 DE 3577725D1 DE 8585901488 T DE8585901488 T DE 8585901488T DE 3577725 T DE3577725 T DE 3577725T DE 3577725 D1 DE3577725 D1 DE 3577725D1
- Authority
- DE
- Germany
- Prior art keywords
- expression vectors
- high copies
- copies
- vectors
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB848407498A GB8407498D0 (en) | 1984-03-22 | 1984-03-22 | High copy number expression vectors |
PCT/GB1985/000110 WO1985004419A1 (en) | 1984-03-22 | 1985-03-21 | High copy number expression vectors |
Publications (1)
Publication Number | Publication Date |
---|---|
DE3577725D1 true DE3577725D1 (de) | 1990-06-21 |
Family
ID=10558531
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE8585901488T Expired - Fee Related DE3577725D1 (de) | 1984-03-22 | 1985-03-21 | Expressionsvektoren mit hoher kopienanzahl. |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0179786B1 (de) |
JP (1) | JPS61502023A (de) |
DE (1) | DE3577725D1 (de) |
GB (1) | GB8407498D0 (de) |
WO (1) | WO1985004419A1 (de) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ210501A (en) * | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
US4703008A (en) * | 1983-12-13 | 1987-10-27 | Kiren-Amgen, Inc. | DNA sequences encoding erythropoietin |
KR850004274A (ko) * | 1983-12-13 | 1985-07-11 | 원본미기재 | 에리트로포이에틴의 제조방법 |
AU600885B2 (en) * | 1984-05-25 | 1990-08-30 | Zymogenetics Inc. | Stable DNA constructs |
US5256568A (en) * | 1990-02-12 | 1993-10-26 | Regeneron Phamaceuticals, Inc. | Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers |
EP0859057B1 (de) * | 1996-03-13 | 2005-06-15 | Takara Bio Inc. | Plasmid |
CN114450408A (zh) | 2019-09-25 | 2022-05-06 | 鲁汶天主教大学 | 大载体和用于高产量生产的方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
HU201116B (en) * | 1982-09-16 | 1990-09-28 | Benzon As Alfred | Process for producing plasmids having uncontrolled replication conduct under given circumstances |
CA1209501A (en) * | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
-
1984
- 1984-03-22 GB GB848407498A patent/GB8407498D0/en active Pending
-
1985
- 1985-03-21 EP EP85901488A patent/EP0179786B1/de not_active Expired - Lifetime
- 1985-03-21 JP JP50132085A patent/JPS61502023A/ja active Pending
- 1985-03-21 WO PCT/GB1985/000110 patent/WO1985004419A1/en active IP Right Grant
- 1985-03-21 DE DE8585901488T patent/DE3577725D1/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP0179786B1 (de) | 1990-05-16 |
WO1985004419A1 (en) | 1985-10-10 |
GB8407498D0 (en) | 1984-05-02 |
JPS61502023A (ja) | 1986-09-18 |
EP0179786A1 (de) | 1986-05-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
FI852774L (fi) | Avvatningsband foer pressar i en pappersmaskins vaotparti. | |
FI843385A0 (fi) | Anordning i virvelbaeddsreaktor. | |
FI850393L (fi) | Anordning beroerande torkpartiet i en pappersmaskin. | |
FI851035L (fi) | Oevre formningsvira i en pappersmaskin. | |
DE3577725D1 (de) | Expressionsvektoren mit hoher kopienanzahl. | |
FI845087A0 (fi) | Faestuppstaellning foer en skaerkonstruktion i en motorsaog. | |
FI850085A0 (fi) | Aendaggregat foer extrusionsmunstycket i en plastpelleteringsanordning. | |
FI850623L (fi) | Anordning foer kraftalstring i en stroemmande vaetska. | |
FI851958L (fi) | Braensleinsprutningsanordning i en foerbraenningsmotor. | |
ATE52804T1 (de) | Expressionsvektoren mit hoher kopienanzahl. | |
FI842051A0 (fi) | Daempningsventil foer kopplingen i en traktors kraftoeverfoeringsaxel. | |
FI851805A0 (fi) | Foerskjutbar skiva foer floedeskontrollanordningen i en enkel kontrollblandventil. | |
FI853246A0 (fi) | Bandstruktur avsedd foer fastspaenning i flaens. | |
FI850203L (fi) | Anordning i maxtermostat foer kombinationspanna. | |
FI850511L (fi) | Krets foer signalbehandling i en bildvisningsapparat. | |
FI842050A0 (fi) | Banformningsparti i pappersmaskin. | |
FI843081A (fi) | Dubbelviraformare i pappersmaskin. | |
FI841579A0 (fi) | Enkel effektbraennare foer fast braensle. | |
FI843383A (fi) | Ventilhjaelpanordning foer luftslang i cykel. | |
FI841278A0 (fi) | I lastkorg monterad transportoer. | |
FI841924A0 (fi) | Uppstaellning i en vaexellaoda. | |
FI842111A0 (fi) | Straengfoerankringen i en vacket. | |
FI850137A0 (fi) | Avtryckningssystem foer alarmanordning i fordon. | |
FI842118A (fi) | Svaengbar regleringsport i barkningstrumma. | |
FI842126A (fi) | Laosanordning foer en aorbrommsregulator i roddanordning. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |