DE3563066D1 - Displacement polynucleotide assay method and polynucleotide complex reagent therefor - Google Patents

Displacement polynucleotide assay method and polynucleotide complex reagent therefor

Info

Publication number
DE3563066D1
DE3563066D1 DE8585303175T DE3563066T DE3563066D1 DE 3563066 D1 DE3563066 D1 DE 3563066D1 DE 8585303175 T DE8585303175 T DE 8585303175T DE 3563066 T DE3563066 T DE 3563066T DE 3563066 D1 DE3563066 D1 DE 3563066D1
Authority
DE
Germany
Prior art keywords
polynucleotide
assay method
displacement
complex reagent
reagent therefor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8585303175T
Other languages
English (en)
Inventor
Mary Collins
Edward Francis Fritsch
Jon Ira Williams
Joseph Grant Brewen
Steven Elliot Diamond
Marian Sue Ellwood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Institute LLC
Allied Corp
Original Assignee
Genetics Institute LLC
Allied Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/607,885 external-priority patent/US4766062A/en
Application filed by Genetics Institute LLC, Allied Corp filed Critical Genetics Institute LLC
Application granted granted Critical
Publication of DE3563066D1 publication Critical patent/DE3563066D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
DE8585303175T 1984-05-07 1985-05-03 Displacement polynucleotide assay method and polynucleotide complex reagent therefor Expired DE3563066D1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/607,885 US4766062A (en) 1984-05-07 1984-05-07 Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US06/684,308 US4766064A (en) 1984-05-07 1984-12-20 Displacement polynucleotide assay employing polyether and diagnostic kit

Publications (1)

Publication Number Publication Date
DE3563066D1 true DE3563066D1 (en) 1988-07-07

Family

ID=27085616

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8585303175T Expired DE3563066D1 (en) 1984-05-07 1985-05-03 Displacement polynucleotide assay method and polynucleotide complex reagent therefor

Country Status (4)

Country Link
US (1) US4766064A (de)
EP (1) EP0167238B1 (de)
CA (1) CA1247993A (de)
DE (1) DE3563066D1 (de)

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US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
EP0221108B1 (de) * 1985-05-02 1993-09-01 Genetics Institute, Inc. Verfahren und aufbau einer nukleinsäure zur erzeugung von reagenzienkomplexen zur verwendung bei der bestimmung von zielnukleotidsequenzen
WO1987003911A1 (en) * 1985-12-17 1987-07-02 Genetics Institute, Inc. Displacement polynucleotide method and reagent complex
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
ES2025556T3 (es) * 1986-12-01 1992-04-01 Molecular Biosystems, Inc. Metodo del ensayo de hibridacion para detectar una secuencia diana polinucleotidica especifica.
AU619170B2 (en) * 1987-01-09 1992-01-23 Abbott Laboratories Diagnostic assays using nucleic acid probes
EP0703296B1 (de) * 1988-09-29 1998-07-22 Chiron Corporation Nachweis eines Polynukleotids durch Ersätzung einer Kette an einer Fangsonde
US5766933A (en) * 1989-04-26 1998-06-16 Diagnostic Products Corporation Method and element for measuring analytes in biological fluids using immobilized binder--analyte labeled complex
US5116727A (en) * 1989-08-31 1992-05-26 Iniziative Marittime 1991, S.R.L. Hybridization method and composition with alternating polysaccharide of sulfated N-acetylgalactosamine
JPH03123500A (ja) * 1989-10-06 1991-05-27 Canon Inc 核酸の分別方法
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
CA2037349C (en) * 1990-03-26 2008-06-17 James G. Wetmur Branch migration of nucleotides
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5569582A (en) * 1991-07-15 1996-10-29 Institute Of Molecular Biology & Technology Rapid amplification and detection of nucleic acids
EP0600936B1 (de) * 1991-07-29 1999-10-06 Serex, Inc. Differentielle bindungsaffinitäten und darauf beruhende dissoziationstestverfahren
US5445933A (en) * 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US5710009A (en) * 1994-02-17 1998-01-20 Serex, Inc. Receptor:release ligand (reland) complexes and release assays using said reland and methods and kits based thereon
US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
EP0826066B1 (de) 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US5747256A (en) * 1995-12-19 1998-05-05 Beckman Instruments, Inc. Homogeneous DNA probe titration assay
US6121008A (en) * 1996-03-20 2000-09-19 Serex, Inc. Chromatographic immunoassay device and method utilizing particle valency for quantification
WO1999024612A2 (en) 1997-11-06 1999-05-20 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing
EP1034040A2 (de) 1997-11-25 2000-09-13 Mosaic Technologies Vorrichtung und verfahren zur detektion von zielmolekülen in biologischen proben
WO1999066078A1 (en) 1998-06-18 1999-12-23 Mosaic Technologies Denaturing gradient affinity electrophoresis and methods of use thereof
JP2002518026A (ja) * 1998-06-19 2002-06-25 エムティー テクノロジー， インコーポレイテッド Ｓｒｐｒｎａを用いる非ウイルス生物の検出
WO2000001850A2 (en) * 1998-07-02 2000-01-13 Gen-Probe Incorporated Molecular torches
AU6412799A (en) 1998-10-05 2000-04-26 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
ATE448324T1 (de) * 1999-02-12 2009-11-15 Gen Probe Inc Schutzsonden
SE0000546D0 (sv) * 2000-02-18 2000-02-18 Sven Oscarsson Method for the positioning of marcromolecules and particles
US7005265B1 (en) 2002-06-20 2006-02-28 Wenhong Fan Nonenzymatic catalytic signal amplification for nucleic acid hybridization assays
WO2008029295A2 (en) * 2006-06-30 2008-03-13 Rosetta Genomics Ltd A method for detecting nucleic acids
US7968650B2 (en) * 2006-10-31 2011-06-28 Johnson & Johnson Vision Care, Inc. Polymeric compositions comprising at least one volume excluding polymer
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment

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Publication number Priority date Publication date Assignee Title
US3798320A (en) * 1970-09-18 1974-03-19 South African Inventions Precipitation of bacterial cells with polymers
IL49685A (en) * 1976-05-31 1978-10-31 Technion Res & Dev Foundation Specific binding assay method for determining the concentration of materials and reagent means therefor
IT1114861B (it) * 1977-05-12 1986-01-27 Sclavo Inst Sieroterapeut Metodo per la determinazione del contenuto di costituenti di fluidi biologici e mezzi adatti allo scopo
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US4395486A (en) * 1981-08-19 1983-07-26 Medical College Of Ga. Research Inst., Inc. Method for the direct analysis of sickle cell anemia
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US4434236A (en) * 1982-10-20 1984-02-28 E. I. Du Pont De Nemours & Co. Immunoassay wherein labeled antibody is displaced from immobilized analyte-analogue
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
CA1222680A (en) * 1983-07-05 1987-06-09 Nanibhushan Dattagupta Testing dna samples for particular nucleotide sequences
IL71715A0 (en) * 1983-08-05 1984-09-30 Miles Lab Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization
EP0139489A3 (de) * 1983-09-26 1988-01-13 Ortho Diagnostic Systems Inc. Sandwichhybridisierungsverfahren zum Nachweis von Nukleinsäuren
US4629689A (en) * 1984-08-29 1986-12-16 Allied Corporation Binding assay with amplified read-out and gas-phase detection

Also Published As

Publication number Publication date
EP0167238A1 (de) 1986-01-08
CA1247993A (en) 1989-01-03
EP0167238B1 (de) 1988-06-01
US4766064A (en) 1988-08-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee