DE3324825A1 - METHOD FOR WASHING THE REACTION TUBES FOR IMMUNOLOGICAL TESTS - Google Patents

Description

BEHRINGWERKE AKTIENGESELLSCHAFT HOE 83 / B 009 D.t> h. HS / sch

Procedure for washing reaction vessels for immunological tests

The invention relates to a method for washing the reaction vessels for immunological tests using washing liquid.

There is a special feature of immunological tests, such as the enzyme-linked immunosorbent assay (ELISA) therein, ^ at certain intermediate steps during implementation of the test, non-specifically bound material is removed by washing with suitable washing liquids must become. If the washing process is inadequate in its effectiveness, certain ones can be used after the end of the test Nonspecificity reactions, decrease in sensitivity and deterioration the reproducibility of results was determined will.

*)that

The effectiveness of washing in immunological tests is determined by a number of factors, for example Detergent content of the washing liquid, number of successive washing cycles, shape of the reaction vessel or speed of suction of the washing liquid. The most important parameter, however, is the length of stay the washing solution in the individual reaction vessels before Suction.

When using semi or fully automatic washing machines the retention time of the washing liquid in the reaction vessel is approx. 40 seconds with four washing cycles. An increase the number of times of washing does not generally improve the washing efficiency. Tests with sample material high consistency, e.g. supernatant from stool samples, According to the prevailing opinion, they basically require a longer dwell time for the washing solution, but they have the capacity DO3: VJaschgeräte would greatly reduce. Additional washing

It has been proven that passes no longer bring about any improvement in these tests, but rather worsen due to the increased Detergent consumption the test costs.

The aim of the invention is therefore, even with difficult sample material, with the usual washing cycles and dwell times do without deterioration in the test result.

The object is achieved by the invention in that the washing liquid at an angle (0 £) of 50 ° to 80 ° is passed to the Viand of the reaction vessels. An improvement can still be achieved if the washing liquid is directed eccentrically with respect to the longitudinal axis of the reaction vessels on the wall.

Of course, care must be taken that when the washing liquid is introduced, neither by rebounding on the Splashes occur on the wall of the reaction vessel while the washing liquid still sloshes out of the reaction vessel. The flow velocity and location of the impact of the washing liquid on the wall should therefore be coordinated with one another be. The movement of the washing liquid in the reaction vessel generated according to the measures of the claims, which is comparable to that in a cyclone, becomes a previously unattainable washing result with the same expenditure of time and the same amounts of washing liquid achieved as in the known method.

The method according to the invention is explained in more detail below with reference to the figures showing only one embodiment explained. It show in schematic representation Figure 1 shows the supply of the washing liquid in side view and

Figure 2 in plan view.

The Waschf lüssigkeit (1) is et by means of nozzle (2) at an angle in item (3) ui: the wall of the Reaktionsgcfäßcb (Ί)

directed. The eccentric supply of the washing liquid, as shown in FIG. 2, is particularly advantageous.

Claims (2)

Patent claims: -A - HOE 83 / B 009 Procedure for washing the reaction vessels for immunological Tests by means of washing liquid, characterized in that the washing liquid is at an angle (cx>) from 50 ° to 80 ° on the wall of the reaction vessels is directed. 2. The method according to claim 1, characterized in that the washing liquid is passed onto the wall acentrically with respect to the longitudinal axis of the reaction vessels.