DE29824483U1 - Osteoblasts or their precursors - Google Patents
Osteoblasts or their precursorsInfo
- Publication number
- DE29824483U1 DE29824483U1 DE29824483U DE29824483U DE29824483U1 DE 29824483 U1 DE29824483 U1 DE 29824483U1 DE 29824483 U DE29824483 U DE 29824483U DE 29824483 U DE29824483 U DE 29824483U DE 29824483 U1 DE29824483 U1 DE 29824483U1
- Authority
- DE
- Germany
- Prior art keywords
- osteoblasts
- precursors
- bone
- cord blood
- umbilical cord
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000000963 osteoblast Anatomy 0.000 title claims description 16
- 239000002243 precursor Substances 0.000 title claims description 12
- 210000004700 fetal blood Anatomy 0.000 claims description 10
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 230000003169 placental effect Effects 0.000 claims description 2
- 210000000988 bone and bone Anatomy 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 5
- 238000002054 transplantation Methods 0.000 description 4
- 210000001185 bone marrow Anatomy 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000011164 ossification Effects 0.000 description 2
- 208000010392 Bone Fractures Diseases 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 238000011316 allogeneic transplantation Methods 0.000 description 1
- 230000008468 bone growth Effects 0.000 description 1
- 239000000316 bone substitute Substances 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 208000002865 osteopetrosis Diseases 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 210000003954 umbilical cord Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Microbiology (AREA)
- Rheumatology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Prostheses (AREA)
Description
Osteoblastenvorläufer und Knochenbildung aus humane NabelschnurblutzellenOsteoblast precursors and bone formation from human umbilical cord blood cells
BeschreibungDescription
Anwendungsgebietfield of use
Die Erfindung betrifft ein Verfahren entsprechend dem Oberbegriff des Anspruchs 1.The invention relates to a method according to the preamble of claim 1.
Stand der TechnikState of the art
Verzögerte oder ausbleibende Konsolidierung von Frakturen sowie die Rekonstruktion knöcherner Defekte stellen sowohl aus chirurgischer als auch aus ökonomischer Sicht eine große Herausforderung dar. Da für au.tologe Transplantationen die Ressourcen begrenzt sind und ein nicht zu vernachlässigendes Risiko bei Zweiteingriffen zur Gewinnung von körpereigenem Knochenmaterial besteht, wird seit jeher nach Alternativen zur autologen Spongiosatransplantation gesucht (1). Die Vorläufer reifer Osteoblasten finden sich in Knochenmark und können aus diesem erfolgreich isoliert und angereichert werden. Bekanntermaßen stellt plazentares Restblut aus der Nabelschnur Neugeborener eine Alternative zu Knochenmark als Stammzellpool der Hämatopoese dar (2). Bislang wurde Existenz von Osteoblasten oder deren Vorläufer im Nabelschnurblut nicht beschrieben.Delayed or non-existent consolidation of fractures and the reconstruction of bone defects represent a major challenge from both a surgical and an economic point of view. Since resources for autologous transplants are limited and there is a non-negligible risk in secondary interventions to obtain the body's own bone material, alternatives to autologous cancellous bone transplantation have always been sought (1). The precursors of mature osteoblasts are found in bone marrow and can be successfully isolated and enriched from it. It is known that placental residual blood from the umbilical cord of newborns represents an alternative to bone marrow as a stem cell pool for hematopoiesis (2). To date, the existence of osteoblasts or their precursors in umbilical cord blood has not been described.
Nachteile des Standes der TechnikDisadvantages of the state of the art
Bei der autologen Spongiosaplastik sind die Ressourcen begrenzt. Weiterhin steht die Operation ein nicht zu vernachlässigendes Risiko dar und erhöht als Zweiteingriff die Traumatisierung des Patienten.In the case of autologous cancellous bone grafting, resources are limited. Furthermore, the operation poses a significant risk and, as a second procedure, increases the patient's trauma.
Aufgabe der ErfindungObject of the invention
Alternatives Knochenwachstum (Ex vivo und In vivo).Alternative bone growth (ex vivo and in vivo).
Lösung der ErfindungSolution of the invention
Osteopoese durch Osteoblasten oder deren Vorläufern, die aus humanen Nabelschnurblutzellen gewonnen wurden.Osteopoiesis by osteoblasts or their precursors derived from human umbilical cord blood cells.
Vorteile der ErfindungAdvantages of the invention
Im Gegensatz zu den begrenzten Ressourcen der autologen Spongiosaplastik sind durch die Existenz von Nabelschnurblut-Banken die Ressourcen für eine Transplantation von Osteoblasten und Osteoblastenvorläufern nahezu unbegrenzt vorhanden. Durch die große Zahl an vorhandenen asservierten Nabelschnurblut-Transplantaten ist es weiterhin möglich, HLA-angepaßte Osteoblasten und Osteoblastenvorläufer einzusetzen, um etwaige Probleme bezüglich der Gewebeverträglichkeit zu minimieren. Desweiteren bestehen durch den Ersatz autologer Transplantationen von Spongiosa durch die allogene Transplantation von Osteoblasten und deren Vorläufern aus Nabelschnurblut ökonomische Vorteile, da ein Zweiteingriff zur Entnahme der Spongiosa entfällt. Weiterhin besteht eine geringere Traumatisierung des Patienten.In contrast to the limited resources of autologous cancellous bone grafting, the existence of cord blood banks means that the resources for transplantation of osteoblasts and osteoblast precursors are almost unlimited. The large number of cord blood transplants available means that it is still possible to use HLA-matched osteoblasts and osteoblast precursors in order to minimize any problems with tissue compatibility. Furthermore, replacing autologous cancellous bone transplantation with allogeneic transplantation of osteoblasts and their precursors from cord blood offers economic advantages, as a second operation to remove the cancellous bone is not necessary. Furthermore, the patient is less traumatized.
BeispielbeschreibungExample description
Ex vivo ist es gelungen, Vorläuferzellen von Osteoblasten aus Nabelschnurblut zur Ausreifung in Osteoblasten zu stimulieren und diese anschließend zu expandieren. Damit können Osteoblasten und deren Vorläufer aus Nabelschnurblut als Eigen- oder Fremdtransplantat (gewebeverträglich) zur Osteogenese z.B. bei Osteopetrosepatienten dienen. Dabei können die Zellen durch Infusion den entsprechenden Patienten zugeführt werden. Hierzu können die Osteoblasten aus den entsprechenden Vorläuferzellen in vitro generiert oder expandiert werden. Dadurch wird eine wesentliche Verbesserung bei der Heilung von Altersbrüchen, bei Unfall-bedingten Knochenbrüchen, bei Schließung von Knochendefekten oder Skelettverlängerungen erreicht.Ex vivo, it has been possible to stimulate precursor cells of osteoblasts from umbilical cord blood to mature into osteoblasts and then expand them. This means that osteoblasts and their precursors from umbilical cord blood can be used as autologous or allogeneic transplants (tissue-compatible) for osteogenesis, e.g. in osteopetrosis patients. The cells can be administered to the relevant patients by infusion. For this purpose, the osteoblasts can be generated or expanded in vitro from the relevant precursor cells. This leads to a significant improvement in the healing of age-related fractures, accident-related bone fractures, closing of bone defects or skeletal extensions.
LiteraturstellenReferences
(1) J.M. Rueger 1996 Knochenersatzmittel State of the Art und: Wohin gehen wir? Unfallchirurg 99: 228-236(1) J.M. Rueger 1996 Bone substitutes: State of the art and: Where are we going? Unfallchirurg 99: 228-236
(2) Broxmeyer HE, Hangoc G, Cooper S, Ribeiro RC, Graves M Yoder M, Wagner J, Vadhan Raj S, Benninger L, Rubinstein R et al. 1992 Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults. Proc Natl Acad Sei U S A 89(9): 4109-13. (2) Broxmeyer HE, Hangoc G, Cooper S, Ribeiro RC, Graves M Yoder M, Wagner J, Vadhan Raj S, Benninger L, Rubinstein R et al. 1992 Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults. Proc Natl Acad Sci U S A 89(9): 4109-13.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE29824483U DE29824483U1 (en) | 1998-01-29 | 1998-01-29 | Osteoblasts or their precursors |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE29824483U DE29824483U1 (en) | 1998-01-29 | 1998-01-29 | Osteoblasts or their precursors |
| DE19803267A DE19803267A1 (en) | 1998-01-29 | 1998-01-29 | New method of osteogenesis uses osteoblasts and osteoblast precursors obtained from placental blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE29824483U1 true DE29824483U1 (en) | 2001-12-13 |
Family
ID=26043406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE29824483U Expired - Lifetime DE29824483U1 (en) | 1998-01-29 | 1998-01-29 | Osteoblasts or their precursors |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE29824483U1 (en) |
-
1998
- 1998-01-29 DE DE29824483U patent/DE29824483U1/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R207 | Utility model specification |
Effective date: 20020124 |
|
| R150 | Utility model maintained after payment of first maintenance fee after three years |
Effective date: 20021126 |
|
| R151 | Utility model maintained after payment of second maintenance fee after six years |
Effective date: 20040218 |
|
| R158 | Lapse of ip right after 8 years |
Effective date: 20060801 |