DE2802396A1 - Animal feed mixt. contg. proteolytic enzyme - and antibiotic of tetracycline type (NL 24.7.79) - Google Patents

Abstract

Animal feed based on carbohydrates, protein, fats and opt. other additives additionally contains 1-250 ppm tetracycline, chlorotetracycline or oxytetracycline, and enough proteolytic enzyme (I) to provide an activity of 0.05-2.5 m TU/g. (I) is esp. an acid protease active within the pH range 2.5 - 6.5. Used for feeding poultry, pigs, calves etc.. This combination of additives further improves the growth rate.

Description

Animal feed mix

Subject of the main patent (patent application P .. .. ..... (D 5724) is an animal feed mixture based on carbohydrates, protein, fats and possibly customary additives, characterized by a content of 1 - 250 ppm of one of the antibiotics lincomycin, oleandomycin and such an amount proteolytic enzymes that an enzymatic activity of o, o5 - 2.5 n.TU / g is present.

It has been found that high-quality animal feed mixtures can then also be used with a corresponding growth-improving effectiveness, if one obtains the antibiotics of the Efauptpatent through one of the antibiotics tetracycline, chlortetracycline or Oxytetracycline replaced.

The invention accordingly relates to an animal feed mixture on the basis of carbohydrates, protein, fats and, if necessary, the usual additives according to the main patent (patent application P .. .. ..... (D 5724), characterized by a content of 1 - 250 pprn of one of the antibiotics tetracycline, chlortetracycline or oxytetracycline and such a ring of proteolytic enzymes that one enzymatic activity of 0.05 - 2.5 mTU / g is present.

The antibiotics mentioned belong to the group of quinone antibiotics and are obtained in a known manner by fermentation from the following Streptomyces strains Convenient: Tetracycline from Streptotryces viridifaciens Chlortetracycline from Streptomyces aureofaci * ns Oxytetracycline from Streptomyces rimosus. The empirical sum formulas the antibiotics are: Tetracycline: C22H24N2O8, Chlortetracycline C22H23 @@@ 2O8, Oxytetracycline: C22H24N2O9.

The antibiotics mentioned are also present inside as hydrochlorides.

Suitable proteolytic enzymes are mainly obtained by growing Microorganisms and separation from the nutrient solution;. (- s obtained.

The procedures for this are known. For example, be proteolytic Enzymes from Bacillus licheniformis, Bacillus natta, Bacillus subtilis, etc. are used. Acid proteases, e.g. B. from Pspergillus niger or from Rhizopus rhizopodiformis according to patent application P 25 28 490.6. Such proteases have a particularly broad spectrum of activity in the weakly acidic range between pH 2.5 - 6.5.

Commercially available feed mixes are for special needs of the individual animal species as optimally compiled as possible. Form the basis Charcoal mainly from cereal components, maize or the like. As a protein carrier serve primarily soy meal, fish meal, carcass meal, bran and the like. Missing amino acids, e.g. B. methionine or lysine can also be added. Fats are added in the form of vegetable or animal fats or waste fats. Salts such as calcium phosphate, calcium carbonate and table salt are also used to build up the body added. If necessary, the feed mixture is refined by adding trace elements, Vitamins, fiber, etc. rounded off. Also substances produced by fermentation such as single-cell proteins from petroleum fractions or alcohols, various ileves, algae protein or other substances, possibly also obtained from waste materials, can e.g. T. be part of the feed formulation to a considerable extent.

The 5'ierfuttermischungeIl according to the invention contain in addition to the usual An additional 1 - 250 components for each specific animal species ppm, preferably 1o - loo ppm of one of the antibiotics mentioned, depending on the age of the Animal and the type of feed mix, and proteolytic enzymes in such an amount that the enzymatic activity is 0.05-2.5 n.TU / g, preferably 0.2-0.5 mTU / g.

The additive concentrations mentioned refer to the content in total feed. Concentrates are the same for concentrates or supplementary feeds correspondingly higher. In particular, a combination of one of the antibiotics mentioned is used and an acidic fungal protease or protease mixture is used. The one achieved with it The increase or improvement in feed conversion is also several percent in comparison to corresponding control mis containing only antibiotics chungen.

The claimed combination of one of the antibiotics mentioned and Enzymes are particularly useful in poultry, pig and calf feed. However, it can also be used in feed mixes for other animal species, in which one of the antibiotics itself is used to advantage.

The antibiotic is expediently added to the feed in the form of a premix added, e.g. B. bound to soybean meal.

When using farinaceous feed compositions is also the enzyme component is applied as a premix. A acts as a carrier substance any feed component, e.g. B. also soy meal or an inert carrier.

When using steam pelleted feed, the addition of enzymes must be added made in suitable stabilized porn in order to deactivate it through moisture and prevent heat during pelleting. Such a method is e.g. B. Subject of the patent .. .. .... . (Patent application P 26 O2 260.6).

The proteolytic activity is used to determine the enzyme unit (TU) the protease is determined according to the well-known principle of Anson: a suitably diluted one Amount of enzyme solution is at 40 ° C for 20 minutes with an equal volume of a 1.2 % casein solution incubated, this o, 6% lactic acid, 6 mol urea and Contains 0.1 mole of citric or acetic acid. The pH of the casein solution is determined by Addition of 2 N sodium hydroxide solution adjusted to 4.5. After the incubation is done in volume ratio 1: 1 mixed with 0.4 N trichloroacetic acid, the precipitate formed of The undigested casein is filtered off and the protein fragments resulting from the breakdown are in the filtrate determined by any protein determination method. Suitable for this is z. B. the method described by Läyne in Method of Enzymology 3 (1957) page 448 gg.

A blank value must be prepared for each test sample, first of all Trichloroacetic acid and then casein solution is added. This blank value gives besides the reagent blank value shows the proportion of low molecular weight peptides that has already been is present in the enzyme solution prior to digestion. The difference between main and blank value is then compared with the extinction using the specified method, which supplies a certain amount of tyrosine in this determination. That amount of tyrosine is then a measure of the proteolytic activity of the enzyme present: a Enzyme unit (TU) is the amount of enzyme that a casein solution in a Minute releases cleavage products that have the same extinction as a 1 M tyrosine solution. The specification in mTU = lo 5 TU is usual.

Example 1 672 day-old broiler chicks of the Lohmann origin were 4 weeks old For a long time under the conditions of the battery husbandry, identical feed mixtures were ridden in each case fed, but a) without the addition of antibiotics or enzyme b) with the addition of 30 ppm tetracycline c) with the addition of 30 ppm tetracycline and 0.3 mTU / g feed acid Proteases from Aspergillus niger d) with the addition of 50 ppm chlorotetracycline e) with Addition of 50 ppm chlorotetracycline and 0.45 mTU / g feed acidic proteases from Rhizopus rhizopodiformis f) with the addition of 20 ppm oxytetracycline g) with the addition of 20 ppm Oxytetracycline and 0.27 mTU / g feed acidic proteases from Rhizopus rhizopodiformis and Aspergillus niger. At the end of the experiment, the final weight of the animals and determines the feed conversion rate.

"Feed conversion" shows the ratio of feed consumption: increase Understood.

Feed composition (%): corn 55.27 soy as chrot 25, oo corn gluten 12, oo soybean oil 4.00 dicalcium phosphate 2.00 carbonated fodder lime o.84 cattle salt 0.20 Sodium sulfate 0.20 Dl-methionine 0.15 Vitamin-mineral premix 0.311 loo, oo Result: a) b) c) without addition with tetracycline with tetracycline + enzyme final weight 754 g 782 g 798 g Feed conversion 1.59 1.60 1.55 a) d) e) without addition with chlorotetramine or chlorotetracycline cyclin + enzyme final weight 754 g 786 g 803 g feed conversion 1.59 1.61 1.58 a) f) g) without addition of oxytetra- m. oxytetracycline cyclin + enzyme final weight 754 g 787 g 801 g Feed conversion 1.59 1.58 1.57 Example 2 672 day-old o-masters of origin Lohmann were kept under battery conditions with a for 8 weeks each fed an identical feed mixture, but a) without the addition of antibiotics or enzyme b) with the addition of 15 ppm tetracycline c) with the addition of 15 ppm tetracycline and 0.2 mTU / g feed acidic proteases from Rhizopus rhizopodiformis d) with additive of 10 ppm chlorotetracycline e) with the addition of 10 ppm chlorotetracycline and 0.38 mTU / g feed acidic proteases from Aspergillus newer and Rhizopus rhizopodiformis f) with the addition of 115 ppm oxytetracycline g) with the addition of 45 ppm oxytetracycline and 0.5 mTU / g feed acidic proteases from Aspergillus niger.

The feed composition and the methodology corresponded to Example 1.

Result: a) b) c) without addition with tetracycline with tetracycline + enzyme final weight 2213 g 2256 g 2289 g Feed conversion 2.33 2.31 2.27 a) d) e) without addition - with chlorotetra- m.Chlortetracycline cyclin + enzyme final weight 2213 g 2249 g 2278 g feed conversion 2.33 2.3o 2.25 a) f) g) without addition with oxytetra- m.oxytetracgcline cyclin + enzyme final weight 2213 g 2262 g 2293 E Feed conversion 2.33 2.35 2.28 Example 3 An identical fattening feed was given to 105 pigs in the 30-60 kg weight segment fed, but a) without the addition of antibiotic or enzyme b) with the addition of loo ppm oxytetracycline c) with addition of loo ppm oxytetracycline and 0.4 mTU / g feed acidic proteases from Aspergillus niger and Rhizopus rhizopodiformis The animals were Maintained in groups of 5 on straw floors and according to the DLG ration list twice a day fed.

Feed composition (ß): maize 15 wheat 20 barley 20 oats 1o wheat bran 8 soy meal 24 mineral feed 3 loo result: a) b) c) without addition of r-oxytetracycline m.oxytetracycline + enzyme daily increase 708 g 735 g 752 g feed conversion 2.49 2.42 2.34

Claims (2)

Animal feed mixture (addendum to patent (patent application P .. .. .... . (D 5724)) Claims 1. Animal feed mixture based on Carbohydrates, protein, fats and, if necessary, customary additives according to the patent (Patent application .. .. ... .. (D 5724)), characterized by a content of 1 - 250 ppm of one of the antibiotics tetracycline, chlortetracycline or oxytetracycline and such a close proximity to proteolytic enzymes that an enzymatic activity 0.05 - 2.5 is irTUig available. 2. animal feed mixture according to claim 1, characterized in that the proteolytic enzymes acidic proteases with a spectrum of activity. between pF are 2.5 - 6.5.