DE2758022A1 - Stable liquid catalase compsns. - contg. microbicide as preservative - Google Patents

Abstract

Catalase compsn. according to the Parent Patent comprise aq. solns. or suspensions contg. (a) 0.5-10 wt. % anionic surfactant, (b) 2-50% nonionic surfactant and (c) 500-500,000 Iu/ml of catalase, with an (a) : (b) wt. ratio of 1:1-100. The improvement lies in the fact that the compsns. also contain 0.0005-1 (pref. 0.05-0.5) wt. % of a microbicide. The microbicide acts as a preventive (esp. when the compsns. contain no lower alcohol) without impairing the activity of the catalase.

Description

"Stable, liquid KataLase preparation"

Subject of the main patent (patent application P 27 53 679.4, internal Applicant's file number D 5676) is a stable, liquid containing catalase Enzyme preparation in the form of an aqueous solution or suspension, characterized by a content of A) 0.5 to 10 percent by weight of at least one water-soluble anionic surfactant from the class of sulfonates and / or sulfates in the form of alkali metal, ammonium or organic ammonium salts, B) 2 to 50 percent by weight of at least one nonionic Surfactants from the class of polyglycol ether derivatives, C) catalase in one of these close that the activity of the agent is 500 to 500,000 international units U / ml is, the weight ratio of anionic surfactant (A) to nonionic Surfactant (B) is 1: 1 to 1: 100.

According to claim 6, up to 40 percent by weight can be monovalent and / or polyhydric alcohols or partial ethers of polyhydric alcohols may be present. These additives, especially the monohydric alcohols, have a certain preservative effect Effect.

According to the present additional application, the addition of certain microbicides The shelf life of catalase preparations is improved even in the absence of alcohol. The invention accordingly provides a stable, liquid catalase preparation according to Patent (patent application P 27 53 679.4), characterized by a content of 0.0005 to 1% by weight, preferably 0.05 to 0.5% by weight of a microbicide as In addition to the bacterial catalase mentioned in the main patent, catalase also comes from liver preparations obtained catalase, in particular beef liver catalase, in question. With regard to the Activity or the quantities to be used are those stated in the main application Declarations. The same applies to the type and amount of anionic and nonionic Surfactants and the other additives mentioned there.

The following classes of compounds or

Compounds that do not cause protein denaturation or

are free from acylating groups.

1. Aromatic hydrocarbons such as toluene.

2. Phenols and halogenated phenols, e.g. o-phenylphenol, 2-benzylphenol, p-hydroxybenzoic acid, p-chloro-m-cresol, 4-chloro-3,5-xylenol, 2,4-dichloro-3,5-xylenol, p-chloro-ophenylphenol, 2-benzyl-4-chlorophenol.

Of these, the mononuclear phenol derivatives are primarily used Question, since the antimicrobial activity of the polynuclear is reduced by the nonionic surfactants will.

3. Trichlorocarbanilide.

4. Bromonitro compounds, e.g. 2-bromo-2-nitropropanediol, 5-bromo-5-nitro- 1, 3-dioxane.

5. Organic mercury compounds, e.g. phenyl mercury acetate, Phenyl mercury borate, ethyl mercury thiosalicylate sodium.

Mixtures of different microbicides can also be used.

The microbicides mentioned give the agents according to the main patent greater resistance even in the absence of low molecular weight alcohols, like methanol or ethanol, without impairing the activity of the catalase at the same time.

In the case of preservation with aromatic hydrocarbons, such as Toluene, the agents are particularly suitable for use in rubber and plastics processing.

In addition, the possible uses of the preserved funds the same as stated in the main patent.

Example A product was prepared with the following composition (in% by weight): catalase (activity 550 U / mg) 5.0 alkylbenzenesulfonate 2.5 C16-C13 fatty alcohol + 10 ethylene oxide 10.0 water remainder The agent was 48 hours at room temperature in stored in an opened bottle and then sealed for 10 days in a Stored at a temperature of 30 OC. The number of germs was then 4,105 per ml.

If toluene is added in amounts of 0.05% by weight (experiment 1) or 0.1% by weight (experiment 2) only 2,102 germs (experiment 1) or 40 germs (experiment 2) found.

The alkalase activity was not measurable within the duration of the experiment sunk.

Claims (2)

"Stable, liquid catalase preparation" additional application to the patent application P 27 53 679.4 (D 5676) claims 1. Stable, liquid catalase preparation according to Patent (patent application P 27 53 679.4>, characterized by a content of 0.0005 to 1% by weight, preferably 0.05 to 0.5% by weight of a microbicide. 2. Composition according to claim 1, characterized in that the microbicide from at least one compound from the class of aromatic hydrocarbons, the optionally halogenated phenols, the trichlorocarbanilide, the bromonitro compounds and the organic mercury compounds.