DE2656333C2 - Process for the production of liposomes which contain medicinal substances - Google Patents

Description

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Example 2

Manufacture of preparations for influencing the secretion mechanism and triggering insulin secretion. There are thereby encapsulated substances in liposomes, such as. B. Sugar (glucose, mannose, ribose), hormones (STH, ACTH, glucagon), enteric hormones (secretin, pancreozymin, sulfonylureas, etc.). Leucine, arginine, Amino acid mixtures, ketone bodies, lauric acid, propionic acid, butyric acid, citric acid, cyclicals AMP xylitol with the addition of extracts from the pancreas or the B cells of the same in a concentration of μg to ng per ml. On the other hand, insulin secretion can be inhibited by encapsulating 2-deoxyglucose, Mannoheptulose, diazoxide, adrenaline, and insulin. For example, when using glucose, one starts from 5% glucose which is isotonic in blood. The manufactured Liposomes are impregnated from the outside with appropriate extracts from the pancreas or B cells, or an aqueous solution of these substances is used as the suspension medium, sodium lauryl sulfate be added in μg to ng concentrations can. In an analogous manner, other types of tissue can also be used by adding inducers or inhibitors and cells are affected.

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Claims (1)

1 2 this suspension solution does not need to be removed. Claim: The detergents facilitate oral application both the resorption of the liposomes and that in the sus- Process for the production of liposomes, molecules contained in welfare pensions,
before containing drugs, with cell tropisraus and 5 special application possibilities consist in improved permeability during application, with triggering and, on the other hand, in the inhibition of specific cell and tissue extracts or Fischer cell functions, such as z. B. insulin secretion and membranes isolated from it, enzymes, cell-specific secretion of other hormonal glands by the encapsulated immunoglobulins or specific factors, sulphation of induction substances or inhibitors, if necessary, which correspond to the target cells, to the liposoio in combination with corresponding cell or Organmenmembrane covafcnt or adsorptively coupled 'extracts and impregnation of the liposome membrane are, according to patent P 26 50502, characterized or the use of analogous cytotropic fractions that the lipoid mixture and that of immunoglobulins in the suspension medium (see aqueous solution with a turbo mixer homogeneous game 2 and 3).
be nized. 15th example 1 Liposomes are made from a previous one Mixture of egg lecithin, cholesterol and diethyl 20 phosphate in a molecular mixing ratio of 10: 2: 1 in chloroform. The latter is done in a vacuum The method of the patent P 26 50 5023 enables vaporization. 10 g of this mixed substance are now it. Liposomes as a vehicle for certain target cells heated up to 60 ° C and under constant homogeneity z. B. to use organ cells or tumor cells. Use an Ultra-Turrax stick to record 50 ml of an aqueous at the surface of the lipoid membrane of the liposogenic organ extract from the fetal portion of the bovine me as described in the claim, by ad- pfazenta in a dilution of 10 ~ ⁶ in physiological Sorptive or covalent bond occupied with molecules, shear saline solution added. Homogenization a selective tropism to the corresponding takes place until the lipoid substance becomes a creamy Components of the cell membrane of the target cells have a consistency that has been processed. Zen. 30 wa 10 ° C is now an aqueous suspension solution that The present application relates to what is indicated in the claim as being isotonic or hypertonic to the incorporated solution Object. visually without heating and mechanical pressure The present method is used for relief and stirred. For rinsing, this solution can be repeated by centrifuging the preparation of the liposomes and gation at 120,000 g for 3 hours. The liposo- 35 since become. The liposomes can also be through films, in contrast to the methods that have been mentioned in the tration through a filter with a pore size under the main patent, using half the size of the liposomes, as z. B. is used to sterile a turbo mixer with stator and rotor after the tration, separate from the rinsing solution. With the principle of the Ultra-Turrax produced by using the ^{pore size suitable for 6G 1} C, the heated lipoid mixture can also be removed from the 40 lipoid lumps to be incorporated which have not been processed into an aqueous solution with constant homogenization. On the other hand is set. The non-incorporated solution can be removed by dialysis into a hypertonic saline solution, the filtration or dialysis or according to the previously practiced suspension fluid, and the rinsing removed by methods by centrifugation and the lipo-renewed supply of the suspending agent can be achieved, some with aqueous solutions with the same In the manner of these rinses, the liposomes can also be prepared rem osmotic pressure like the incorporated solution with suitable reagents for coupling with the possibly rinsed several times with the respective removal of the cytotropic substance. For therapy, it is advisable to use blood after rinsing the liposomes, using isotonic solutions for the inner and outer aqueous detergents, e.g. B. to use 1.5 μg sodium lauryl sulfate per g phase of the liposomes. After rinsing the protein or peptide solution or aqueous Organex, the surface of the liposomes is added by adding suitable or antibody sera, also in highly diluted reagents in aqueous solutions for the couplings. Liposome mixtures with creaming with the cytotropic substances prepared dam- or lotio-like consistency, the liposomes can be resuspended in any desired way from the not to the mixing ratio. A concentrated suspension that has come onto its surface can be rinsed free before oral use, before the substances to be coupled are further added to substances with water or isotonic solutions. After coupling, the rinsing is thin. It is advisable to proceed before the prepared in this way. The advantage of the applied homogenization lies Liposomes resuspended in an aqueous solution The rationale is that; it is not necessary to remove the lipoid Liposomes can be in solutions of proteins, oligo mixture as a thin film before the production of the liposomes. and polypeptides, as well as saccharides and organ extracts, making it possible to produce larger or antibody sera with the same or different amounts of liposomes in one operation. suspend organ species as the incorporated molecules. To suspend the liposomes with cell or commute. The addition of detergents, such as. B. Na organ tropism can also be aqueous solutions of trium lauryl sulfate or TWEEN dilutions, which use the 65 organ extracts without causing tropism Liposome membrane not destroyed, facilitates the binding of target cells. The concentration of the aqueous solution formation of such molecules. For the therapeutic use of organ substances is in the μg or pg range Dung, which can be administered parenterally or orally, is rich in per g of dry organ powder.