DE2300251A1 - Rapid bacterial counter - for determining the absolute bacterial content of homogenous liquids eg emulsified milk samples - Google Patents
Rapid bacterial counter - for determining the absolute bacterial content of homogenous liquids eg emulsified milk samplesInfo
- Publication number
- DE2300251A1 DE2300251A1 DE19732300251 DE2300251A DE2300251A1 DE 2300251 A1 DE2300251 A1 DE 2300251A1 DE 19732300251 DE19732300251 DE 19732300251 DE 2300251 A DE2300251 A DE 2300251A DE 2300251 A1 DE2300251 A1 DE 2300251A1
- Authority
- DE
- Germany
- Prior art keywords
- instant
- counting device
- germ
- filter
- germ counting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/04—Dairy products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
Instant-heimzähl-Gerät Zur Bestimmung des absoluten keimgehaltes in homogenen flüssigen Medien. Instant home counting device for determining the absolute germ content in homogeneous liquid media.
Beschreibung Die Arbeitsweise des neuen Gerätes wird an dem Beispiel der Bestimmung des absoluten Keimgehaltes in Rohmilch beschrieben. Description The mode of operation of the new device is illustrated in the example the determination of the absolute germ content in raw milk.
Eine bestimmte Menge, hier 1 ml der zu untersuchenden Rohmilch, wird mittels einer schlauchförmig biegsamen Hi@ette aus dem Vorratsbehälter der milch durch Ansaugen entnommen.A certain amount, here 1 ml of the raw milk to be examined, is using a flexible hose from the milk storage container taken by suction.
Diese probe wird aurch eine schienenartige Vorrichtung über den Auffang-Tricher eines Filtriergerätes gebracht, mit einer bestimmten enge, hier 10 ml, einer erwärmten Emulgatorölsung gemischt und durch ein Membranfilter gesaugt oder gedrückt. Die toren des Filters müssen kleiner als die zu zänlenaen leime sein, Sofort anschließend werden die auf dem Filter zurückbleibenden Keime mit der Lösung eines der Art der Keime angepaßten Farbstoffes markiert und fixiert, indem aus einer zweiten pipette die abgemessene Menge dieser Lösung ebenfalls durch das Filter gesaugt bzw. gedrückt wird.This sample is a rail-like device over the collecting funnel brought a filter device, with a certain narrow, here 10 ml, a heated one Emulsifier oil mixed and sucked or pressed through a membrane filter. the The gates of the filter must be smaller than the glue to be divided, immediately afterwards the germs remaining on the filter with the solution of one of the types of Germ-matched dye is marked and fixed by pulling out a second pipette the measured amount of this solution is also sucked or pushed through the filter will.
Im folgenden Arbeitstakt wird das auf einem fortlaufenden Film angeordnete Filter aus dem Filtriergerät entnommen und in eine Trockenkammer geführt.In the following work cycle, this is arranged on a continuous film Filter removed from the filtering device and placed in a drying chamber.
Gleichzeitig wird ein neues Filter in das Filtergerät befördert und der nächste Vorgang des Filtrierens kann betinnen.At the same time, a new filter is fed into the filter device and the next process of filtering can be carried out.
Die in die Trockenkammer beförderten Filter sind nach erfolgter Trocknung, d.h. bereits nach einigen Minuten sowohl fertig für die weitere Auswertung als auch für eine. Dokumentation fixiert.The filters conveyed into the drying chamber are, after drying, i.e. both ready after a few minutes for further Evaluation as well as for a. Documentation fixed.
Der als jeuheit zu wertende Vorteil dieses Gerätes besteht darin, daß die Entnahme der Probe, die Homogenisierung der zu untersuchenden Flüssigkeit, hier der Rohmilch, sowie die Fixierung, die Markierung und die Auswertung des Keimgehaltes in einem kontinuierlichen Arbeitsgang als Instantverfahren durchgeführt wird.The advantage of this device, which is to be rated as novelty, is that that the taking of the sample, the homogenization of the liquid to be examined, here the raw milk, as well as the fixation, the marking and the evaluation of the germ content is carried out in one continuous operation as an instant process.
Damit ist es erstmalig möglich, unter praktischen bedingungen z.B. unmittelbar bei der Übergabe von Rohmilch vom Landwirt an den Tanksammelwagen die bakteriologische Qualität derselben zu bestimmen und dokumentarisch festzuhalten.This makes it possible for the first time, under practical conditions, to e.g. immediately when raw milk is handed over from the farmer to the tanker truck to determine the bacteriological quality of the same and to record it in a document.
Bei allen bisher üblichen Verfahren zur ermittlung der bakteriologischen Qualität mußten rroben in sterilen behältern entnommen und an ein Speziallaboratorium zur weiterverarbeitung befördert werden. Dabei konnten den wahrheitsgehalt der Untersuchung beeinflussence Fehler, wie eine Keimverschleppung von keimreichen Proben auf keimärmere, sowie Keimvermehrung während des Probentransports nicht immer ausgeschlossen werden.With all the methods used to date to determine the bacteriological Quality samples had to be removed in sterile containers and sent to a special laboratory be transported for further processing. In doing so, the truthfulness of the investigation Influence errors, such as a germ carryover from germ-rich samples to germ-poor, as well as the multiplication of germs during the sample transport cannot always be ruled out.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19732300251 DE2300251A1 (en) | 1973-01-04 | 1973-01-04 | Rapid bacterial counter - for determining the absolute bacterial content of homogenous liquids eg emulsified milk samples |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19732300251 DE2300251A1 (en) | 1973-01-04 | 1973-01-04 | Rapid bacterial counter - for determining the absolute bacterial content of homogenous liquids eg emulsified milk samples |
Publications (1)
Publication Number | Publication Date |
---|---|
DE2300251A1 true DE2300251A1 (en) | 1974-07-18 |
Family
ID=5868259
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE19732300251 Pending DE2300251A1 (en) | 1973-01-04 | 1973-01-04 | Rapid bacterial counter - for determining the absolute bacterial content of homogenous liquids eg emulsified milk samples |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE2300251A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4017398A1 (en) * | 1990-05-30 | 1990-11-15 | Elemer Dr Faltusz | METHOD AND MEANS FOR DETERMINING THE BACTERIOLOGICAL AND CYTOLOGICAL TEXTURE OF RAW MILK |
-
1973
- 1973-01-04 DE DE19732300251 patent/DE2300251A1/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4017398A1 (en) * | 1990-05-30 | 1990-11-15 | Elemer Dr Faltusz | METHOD AND MEANS FOR DETERMINING THE BACTERIOLOGICAL AND CYTOLOGICAL TEXTURE OF RAW MILK |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
OHA | Expiration of time for request for examination |