Field of the invention
The present invention relates to devices for detection (measurement)
of analytes in liquid samples.
Background of the invention
The following background of the invention serves to assist the reader in understanding
The invention is not to be considered as a prior art.
Healthcare is the diagnostic testing of body fluids
an everyday activity. Increasingly, too
Employers, government agencies, sports teams and
other organizations involved in diagnostic testing,
to maintain job security and compliance
To ensure laws, rules and regulations.
It is generally necessary to provide devices for collecting body fluids such as. Urine and to detect the presence of a particular analyte (eg, a drug and / or its metabolite, or a disease indicator). Such test devices generally require that a sample be placed in a sample container and that a technician manually insert and submerge a portion of a test strip into the sample and then pull it out to read the result. Because of the potential contact of the technician with the sample and the associated health and contamination risks, a sealed container to avoid contact is desirable. Various remedies have been proposed to reduce the risk of contact, as described in U.S. Patent No. 4,976,923
, the U.S. Patent No. 5,429,804
and the U.S. Patent No. 6,726,879
which test devices use test strips mounted in their lids. In use, the container is turned over or tilted so that the sample can wet the strips to perform the test.
has the use of test devices by persons other than
Health professionals increased. Due to the fact that these
Tests increasingly carried out by relatively unqualified technicians
and evaluated, the device should be easy to use
to ensure adequate immersion of the test strip
and deliver accurate results.
There is a continuing need for test devices that are a minimum
require manual operation while being accurate and accurate
Ensure reliable test results.
Presentation of the invention
The present invention provides a device for detecting the
Presence of an analyte in a liquid sample
ready. The device has an opening for insertion
the liquid sample into a first chamber for collecting the
liquid sample and a second chamber connected to the first chamber
is connected through a passage and contains a test element.
The device also has a third chamber with the second
Chamber is connected by a channel and a movable element
Contains a first and a second position. The
third chamber is moved from the movable element into a first and second
Divided zone, and the first zone has a ventilation hole.
The movable element is located with at least one wall of the
third chamber in contact to a gas connection between the first
and second zone.
In one embodiment, the device may also include a lid
to close the opening. The test element
may be a sample card containing one or more sample test strips
an embodiment of the device is the movable
Element a piston. The piston may be one with at least one
Wall of the third chamber containing seal in contact
a gas connection between the first and second zone of the third
Chamber to prevent. The third chamber may also have a bottom, and
in one embodiment, the vent hole is located
on the floor of the third chamber. In one embodiment
the piston has a push rod extending to the opening
the container extends. The lid can have a surface
own who touches the push rod and pushes down,
when the lid is applied to the opening.
various embodiments of the device has
the passage has a diameter of less than 10 mm or less
as 9 mm or less than 8 mm or less than 7 mm or less
as 6 mm or less than 5 mm or less than 4 mm or less
than 3 mm.
In another aspect, the present invention provides methods for detecting the presence of an analyte in a liquid sample. The methods include loading a liquid sample into a test device as described herein, causing the movable member to move from the first position to the second position, thereby causing an air volume, from the second chamber to the third chamber to be sucked and thereby cause a volume of liquid to be sucked through the passageway from the first chamber into the second chamber to contact the test element and to detect the presence of the analyte in the sample.
In one embodiment, the methods include touching
the lid with the push rod and the pressing down of the
Push rod when the lid is applied to the device,
and the resulting cause of the movable element,
to move from the first position to the second position.
In one embodiment, this depresses
the push rod and moving the movable member from the
first to the second position, that is a negative air pressure gradient
formed in the third chamber and that an air volume from the
second chamber is sucked into the third chamber. The movement of the
Air volume from the second chamber to the third chamber, the
Cause of the formation of a negative air pressure gradient in the
be the second chamber. With formation of a negative air pressure gradient
is meant that in a chamber forms a vacuum (vacuum),
causing air from another chamber to enter the
Chamber "pulled" to fill the vacuum generated.
The air pressure gradient refers to a difference of the internal
Air pressures between the two chambers.
In one embodiment, the negative air pressure gradient causes
in the second chamber, that the liquid sample from the
first chamber flows through the passage into the second chamber and
while touching the test element. The depressing
the push rod and moving the movable member from the
first position in the second position can cause a
Air volume through the vent hole from the first zone
Presentation of the invention described above is not limiting
and other features and advantages of the invention will become apparent from the following
detailed description and apparent from the claims
Brief description of the drawings
1 is an exploded perspective view of the device according to the present invention.
2 Figure 11 is another exploded perspective view of the device according to the present invention from a different angle.
3 is a perspective view of the device according to the present invention with the lid removed.
4 is a perspective view of the device according to the present invention with the floor removed.
5 Figure 10 is a cross-sectional view of the device of the present invention with a liquid sample received in both the first and second chambers.
6 is an enlarged partial view of the 5 wherein the liquid sample is not shown to more clearly illustrate the structure of a passageway.
7 FIG. 12 is a cross-sectional view of the device according to the present invention showing that a piston is positioned in a first position and the liquid sample is received only in the first chamber. FIG.
8th is an enlarged partial view of the 7 wherein the liquid sample is not shown to more clearly illustrate the structure of a channel.
9 is a cross-sectional view similar to the 7 wherein the piston is positioned in a second position and a portion of the fluid sample has flowed into the second chamber.
The present invention provides apparatus and methods for detection
the presence or amount of an analyte in a liquid
Sample ready. In one embodiment, the device takes
the shape of a cup, which is a liquid sample
for analysis. In one embodiment, the
liquid sample urine. The analyte can be any analyte,
For example, glucose, a drug / drug or a metabolite thereof.
The device uses a system of chambers to move the liquid sample through the device and detect the presence or amount of an analyte in the samples. In one embodiment, the device has three chambers, and the liquid sample can be moved from one chamber to the other by means of air pressure. The surface tension of the liquid sample may be used to hold the liquid in a chamber until the user is ready to move the sample to an adjacent chamber by applying air pressure to the chambers. In various embodiments, the air pressure may take the form of an increase or a decrease (eg, the formation of a negative pressure) of the internal Assume air pressure. The different chambers of the device are connected by passages, channels and vent holes which allow the transfer of air pressure and liquid between the chambers. The device also has a movable element located in one of the chambers. In one embodiment, the movable member is a piston and the chamber is a cylinder in which the piston is housed. As the piston is moved from a first position to a second position, air is forced out of the third chamber and creates a vacuum that is transferred to the second chamber, causing a break in the surface tension of the liquid in the first chamber and thereby causes liquid to flow from the first to the second chamber. By cylinder is meant a structure which contains the movable element and in which the movable element is moved from a first position to a second position. If the chamber is a cylinder, it does not have to have a perfectly cylindrical shape in all embodiments, but may vary in shape and size, or assume one shape on one part and another shape on another part, as long as they are still those of the Performing the assay (s) required functions.
According to one embodiment, the test element is the
Device in the second chamber, and therefore begins the analysis
the liquid sample. In the illustrated in the figures
Embodiment, the movable member has the shape of a
piston-like structure with a generally cylindrical shape. however
may be the movable element in other embodiments
take any suitable form.
A variety of analytes may be detected and quantified by the test elements in the present invention. The analyte may be an infectious agent or an indication of an infected condition. The analyte may be a drug (eg, a drug), a hormone, a protein, a nucleic acid molecule, an ethiological agent or a specific binding pair member. The term "drug of abuse" (DOA) refers to a drug taken for non-medical reasons (usually due to mind altering effects) .The abuse of such drugs can lead to physical and mental damage and (in some substances) to Dependency, addiction and / or death Examples of DOAs include cocaine, amphetamines (eg black beauties, white bennies, dextroamphetamines, dexies, beans), methamphetamines (Crank, Meth, Crystal, Speed), barbiturates ( Valium® , Roche Pharmaceuticals, Nutley, New Jersey); sedatives (ie, sleep aids); lysergic acid diethylamide (LSD); depressants (Downers, Goofballs, Barbs, Blue Devils, Yellow Jackets, Ludes); tricyclic antidepressants (PCA), eg, imipramine, Amitriptiline and doxepin), phencyclidine (POP), tetrahydorcannabinol (THC, kif, marijuana, hash, grass, etc.); and opiates (eg, morphine, opium, codeine, heroin, oxycodone) Reasons are taken at However, where overdose can easily occur can also be tested using these test strips, z. For example, tricyclic antidepressants (imipramine and the like) and over-the-counter products containing acetaminophen.
Test element can be conveniently selected lateral flow test strips
which are widely used to cover a wide range of
To test analytes. However, any suitable test element in the
can be used in the present invention.
Variety of test elements may be in the present invention
be incorporated. One type of test element is a test strip.
Test strips are available in a variety of formats,
such as The immunoassay or chemical assay format, to analytes
of interest in a sample to capture, such. B. a drug
or a metabolite that shows signs of health
gives. Test strips may also be either for non-competitive
or competitive assay formats. For some formats
The test strips have a highly absorbent material with a
Sample application zone, a reagent zone and a test result zone.
The sample is applied to the sample application zone and flows
by capillary action into the reagent zone. Dissolves in the reagent zone
the sample and mixes with the reagents used for detection
of the analyte (if any) are necessary. The sample, now
carries the reagents, flows on to the test result zone.
Additional reagents are immobilized in the test result zone,
such as B. a specific binding molecule for
the analyte. These reagents react with the analyte (if
present) and bind him or one of the first reagents from the
Reagent. Markers for providing the measurable signal
may be present in the reagent zone or in one
separate marking zone.
In non-competitive formats, a signal is generated when the
Sample contains the analyte, and no signal is generated when
the analyte is absent. In competitive formats one can
Signal are generated when no analyte is present, and no
Signal if the analyte is present.
If the test element is a test strip, it can be made from highly absorbent or non-absorbent be produced bierendem material. A test strip may comprise more than one material, which are then in fluid communication. A material of a test strip can be placed on another material of the test strip, such. B. filter paper, which is placed on nitrocellulose. Alternatively or additionally, a test strip may comprise a region of one or more materials followed by a region of one or more different materials. In this case, the regions are in fluid communication and may or may not partially overlap one another. The material or materials of the test strip may be bound to a support or a solid surface, such as. B. a bearing plastic layer to increase their handling strength.
Embodiments in which the analyte by a signal generation system
is detected, such. By one or more enzymes specific to
can react to the analyte, one or more components
of the signal generation system in the same way to the analyte detection zones
of the test strip material, such as those described above
specific binding elements attached to the test strip material
become. Alternatively or additionally, components may be used
of the signal generation system used in the sample application zone, the
Reagent zone or the analyte detection zone of the test strip available
or are contained throughout the test strip, in
one or more materials of the test strip impregnated
become. This can be achieved either by the surface application
solutions of such components or by immersion
of the one or more test strip materials in solutions
such components. After one or more applications or one
or multiple dips becomes the test strip material
dried. Alternatively or additionally
Components of the signal generation system that are present in the sample application zone,
the reagent zone or analyte detection zone of the test strip
are present or contained anywhere in the test strip
are on the surface of one or more test strip materials
of the test strip are applied as for the marked
Reagents has been described.
The zones can be arranged as follows:
Sample application zone, one or more reagent zones, one or more test result determination zones, one or more control zones, one or more adulteration zones and a fluid-absorbing zone. If the test result determination zone has a control zone, it preferably follows the analyte detection zone of the test result determination zone. All of these zones or combinations thereof can be provided on a single strip of a single material. Alternatively, the zones are made of different materials and are connected to each other in fluid communication. For example, the different zones may be in direct or indirect fluid communication. In this case, the different zones may be connected end-to-end to be in fluid communication, overlapped to be in fluid communication, or connected by some other element, such as the like. B. a connecting material, which is preferably highly absorbent, such. As filter paper, glass fibers (glass wool) or nitrocellulose. When using a bonding material, the bonding material may include fluid from the end-to-end bonded zones or materials containing such zones, end-to-end bonded zones, or materials containing such zones that are not in fluid communication, or bonding zones or materials containing such zones contained and overlapped (for example without limitation from top to bottom), but are not in fluid communication transmitted.
Mi relation to 1 and 2 For example, the device of the invention may be used to deliver a body fluid, such as a fluid. B. collecting urine and detecting the presence or amount of an analyte contained therein (eg, a drug and / or a metabolite thereof). The device generally comprises a container 111 with an opening 115 to collect the liquid sample, a lid 101 for closing the opening and at least one test element 137 to do a test. In one embodiment, the lid 101 may be a lid which is screwed tightly onto the device, however, in other embodiments the lid may be snapped or otherwise securely fastened by any suitable means. The device can also be a seal 109 to seal the container when the lid 101 is applied. In the first container 111 a movable element is provided, such. B. a piston 149 that in the tank 111 is movably recorded.
The parts of the device may be molded or cast from any suitable material. For example, a variety of plastics can be used. With reference to the embodiments illustrated in the figures, the device comprises a cup-like main body 111 and a base 151 attached to a floor of the main body 111 hermetically attached. Regarding 3 . 5 . 7 and 9 owns the main body 111 a number of interconnected vertical side walls 117 , where a round top edge 113 connecting the upper ends of the side walls, and a generally flat bottom wall 207 that connects the lower ends of the side walls. In In this embodiment, two inner walls extend 309 generally parallel to the two adjacent side walls 117 and connect to them at their upper ends, so to a second chamber 601 to define that contains the test element. The upper edge 113 defines an opening 115 for introducing the liquid sample and may have threads which, during coupling of the lid to the container 313 fitting with appropriate threads on the lid 101 can intervene.
The device has a movable element which, in certain chambers of the device, can create a negative air pressure when moved from a first to a second position. In the embodiment shown in the figures, the movable element is a piston 149 , A piston housing 111 is inside the main body 111 trained and located with the second chamber 211 containing the test element in air communication. The piston housing 311 forms the third chamber. In this embodiment, the piston housing 311 shaped like a reverse funnel and includes a lower cylindrical section 303 that is on the bottom wall 207 of the main body 111 through a ventilation hole opens. The piston housing also has an upper cylindrical portion 307 , which has a smaller diameter than the lower section 303 owns, as well as a tapered intermediate section 305 that connects the upper and lower sections. As in 7 and detailed in 8th is shown, in this embodiment, the lower portion 303 the piston housing partially with one of the inner walls 309 connected, and a channel 801 is formed in the connection area to allow air exchange between the third chamber 701 and the second chamber 601 to allow. By having the two chambers in "air communication" or "gas communication", it is meant that changing the air pressure in one chamber will cause a change in the air pressure in the other chamber or will cause fluid to escape from a chamber the other is sucked or vice versa, or that air is able to move between the two (or more) chambers.
Chambers of the device can be any shape or design
own, which is suitable to their function in the device
to fulfill. In one embodiment, the
third chamber a generally cylindrical, syringe-like or funnel-shaped shape
but other forms can be used,
the movable element and the first and second zones of the third
Carry chamber. Also, the different zones of the
third chamber take different forms. On similar
Way the second chamber can take any shape, the test elements
carries, and the first chamber can have any shape, the
the introduction of liquid into the device
The bottom wall 207 of the main body (which forms the bottom of the first chamber) contains a passage 205 in it, the first chamber with the second chamber 601 combines. The passage 205 has a diameter that is too small to allow the fluid in the main body 111 to allow it to flow through under atmospheric pressure, which is substantially equal to the atmospheric pressure. Fluid will flow from the first chamber into the second chamber under a negative pressure of air induced by the movement of the movable member. In various embodiments, the diameter of the passage is less than 10 mm or less than 9 mm or less than 8 mm or less than 7 mm or less than 6 mm or less than 5 mm or less than 4 mm or less than 3 mm or even more fewer. In one embodiment, the diameter of the passage is 205 about 4 mm or about 5 mm or about 3 mm or 3 to 5 mm. While in the illustrated embodiment the passage is an opening between the first and second chambers, the passage may also have other configurations. For example, in various embodiments, the passage may be a tortuous (wave-shaped) groove defined in the bottom wall of the main body with a small cross-sectional diameter, or any opening or passage connecting the two chambers. The bottom wall 207 further includes an embossment on its lower surface 209 around the open area.
The base 151 Can be permanent on the main body 111 be attached. The base 151 includes a top wall 153 , a lower wall 213 and a fencing wall 155 that the upper wall 153 and the bottom wall 213 combines. The fencing wall 155 and the bottom wall 213 define a cavity together 157 who is part of the second chamber 601 is and absorbs part of the liquid sample. In this embodiment, a groove 159 in a top of the fencing wall 155 trained to emboss with 209 on the bottom wall 207 of the main body. When assembling the main body 111 and the base 151 can the imprinting 209 in the groove 159 and then ultrasonic welding can be used to hermetically seal these two components. Various other methods may also be used to secure the base and main body together while ensuring a good seal therebetween. In various embodiments, thermal welding, gluing or pressure sealing rings may be used. The base 151 further comprises spaced retaining walls 161 for holding the test element, a block wall 163 to block fast-flowing fluid (rapid rate fluid) and one in the top wall 153 defined ventilation hole 165 , The performance and function of the retaining walls, the block wall and the ventilation hole will be described in detail below.
The lid 101 can with the container 313 coupled to seal the sample in the container. In addition, in the present invention, the coupling of the lid 101 can also be used on the container to begin an assay, which will be described in more detail below. In this embodiment, the lid comprises 101 a top 103 and a downwardly extending flange 105 which has a shape that matches the shape of the edge 113 of the container 313 corresponds so that the flange can be coupled to the edge. In the illustrated embodiment, the top is 103 with a circular depression 107 formed, which has a lower surface 201 owns which the moving element 215 can press down while the lid 101 to the container 313 is coupled. A sealing element 109 , such as B. an O-ring, can between the lid 101 and the container 313 be positioned to provide a seal therebetween.
The test element may be in the form of a test card, which may be any type of device configured to examine a sample. In this embodiment, the test element comprises a probe card (assay card) 119 for detecting the presence or amount of an analyte in the sample, and the apparatus further comprises a falsification test card 131 to record the contamination of the sample. Every card 119 ( 131 ) has such a shaped size and shape that it is in an upper portion 211 the second chamber 601 the device is used and the retaining walls 161 the base 151 is held. In other embodiments, the card may be from any suitable means in the second space 601 being held. In various embodiments, each card 119 ( 131 ) a plate 121 ( 133 ) with a group of slots defined therein 123 ( 135 ), a number of sample strips held in the slots 125 or adulteration strips 137 and a cover 127 ( 139 ) adhered to a surface of the plate and windows 129 ( 141 ) for observing the test result.
The moving element 215 is movable in the piston housing 311 available. In this embodiment, the movable element is a piston 215 , The piston 215 includes an elongated push rod 147 extending through the upper section 307 of the piston housing 311 to the opening 115 of the container 313 extends, a piston body 149 that with one end of the push rod 147 is connected and has a size and shape that is designed to be in the lower section 303 of the piston housing 311 to be mobile, and a first sealing ring 143 , around the push rod 147 is held, as well as a second sealing ring 145 that is around the piston body 149 is held around. In the embodiment shown in the figures, the sealing rings have 143 and 145 the shape of circular rubber or plastic rings. However, the sealing rings may be made of any suitable material and may take any form that functions in the device.
Regarding 5 to 9 is the basis in this embodiment 151 permanently on the main body 111 attached to a one-piece container 313 train. In this assembled state becomes a first chamber 301 inside the main body 111 defined by the opening 115 to store introduced liquid sample. A second chamber 601 of the main body 111 can also have a cavity section 157 include, in part, the base 151 is defined, and an upper section 211 include to receive the test element. A passage 205 is provided to allow the liquid sample from the first chamber 115 in the second chamber 601 to flow. The passage is sized so that the surface tension of the liquid sample will prevent the sample from flowing from the first to the second chamber until it is transferred from the negative pressure created by depressing the piston in the third chamber to the second chamber. " pulled or sucked in. In the present embodiment, the passage is 205 a hole of small size, as previously mentioned.
A third chamber 701 is inside the piston housing 311 defined as in 7 and 9 shown when the piston 215 in the piston housing 311 is received, wherein the first and second sealing ring 143 respectively. 145 serve a seal in the third chamber 701 provide. The third chamber 701 contains a first zone 901 and a second zone 902 wherein the first zone is the area below the movable element and the second zone is the area above the movable element. Therefore, the volume of the first and second zones changes as the movable member is moved from a position to a second position. If present, the seal rings may separate the first zone from the second zone of the third chamber. In the illustrated embodiment, the first zone 901 the space in the third chamber below the second seal ring 145 , and the second zone 902 is the above the first seal ring 145 and below the second seal ring 143 defined space. When the piston 215 in the third chamber 701 moves, remain the first and the second sealing ring 143 . 145 with the inner surface of the upper or lower portion 303 . 307 of the piston housing 311 in contact to provide air communication between the first and second zones 901 . 902 to prevent. The channel 801 allows the passage of air from the second chamber into the third chamber when the movable element in the third chamber is lowered. The channel 801 may take any suitable shape, but is conveniently present as an opening which allows air communication between the second chamber and the third chamber.
methods of use
In use, a liquid sample, such as. As urine, first introduced into the first chamber of the container. The in the first chamber 115 The liquid sample placed will remain in the first chamber and will not pass through 205 because the surface tension of the liquid sample is sufficient to prevent it from flowing through the passage until it is from one in the second chamber 601 vacuum force is sucked through.
The lid 101 will then be on the container 313 coupled, such. B. by screwing the lid on the container. Regarding 7 is the piston 215 at the beginning of the assay (the assay) in a first elevated position. In the first position, the top of the push rod extends 147 in the first chamber a sufficient distance upwards, so that when the lid is applied, the recess 107 in the lid will touch the top of the push rod. The piston body 149 lies in the lower section 303 of the piston housing 311 , In one embodiment, the piston body is located 149 on a number of ribs extending from an inner surface of the intermediate section 305 protrude. When the piston is in the first elevated position, the volume of air is in the second zone 902 the third chamber 701 relatively low in relation to the volume of air in the second zone 902 when the piston body 149 is in the second lowered position.
If the lid 101 screwed onto the container, comes the lower surface 201 the circular depression 107 with the top of the push rod 147 in contact and exerts a downward force on the push rod to cause the movement of the piston along its axial direction from the first raised position to the second lowered position. With the movement of the piston from the first position to the second position, air is expelled from the venting hole from the first zone. At the same time, a negative pressure is generated in the second zone, which causes air from the second chamber 601 is "drawn in" into the second zone of the third chamber to fill the vacuum, thereby also creating a corresponding negative pressure in the second chamber 601 containing the liquid sample in the first chamber 115 through the passage 205 in the second chamber 601 "Pulls" to neutralize the vacuum because the vacuum force is sufficient to overcome the surface tension of the liquid sample at the passage, thus causing the liquid sample to pass the test elements in the upper section 211 touched the second chamber. The vent hole may preferably be designed as an opening that allows the passage of air, but may also take any other suitable design that is consistent with its function. Regarding 5 and 6 In one embodiment, it is a barrier wall 163 located directly in the flow path of the liquid coming from the first chamber into the second chamber. This barrier wall 163 serves to transfer a portion of the liquid sample into the second chamber 601 occurs to block and prevents the liquid sample from reaching a flow rate high enough to cause the strips 125 . 137 damaged or she floods. The barrier wall 163 It requires the liquid to turn at a right angle before it touches the test strips, causing the liquid sample to slow down and, in a more orderly fashion, one end of each strip 125 . 137 touched. This facilitates the capillary action of the liquid sample through the test strip to the test line, and a visible result is displayed 129 . 141 shown. The side walls 117 of the container 313 may be transparent or semitransparent to allow visual observation of the stripes through them.
If, as in 9 shown the piston 211 moved from the first position to the second position, which has in the second chamber 601 aspirated liquid sample has a volume that sufficiently immerses the test strips but does not cause flooding. The piston 211 can be adjusted during manufacture so that movement from the first position to the second position draws in an appropriate amount of liquid sample. In addition, the first position can also be preset during production, but the second position can be controlled by the user. For example, a marker may be used as an indicator on the edge 113 be provided to indicate that when the lid 111 screwed on this line, the piston has reached the second position. In this embodiment, the second position can be achieved when the lid 101 completely on the container 313 is coupled. Thus, the user can begin the assay in a single step of attaching the lid to the container, and the test can be performed.
This example illustrates the general my use of a device of the invention. Water was poured into five devices of the invention designed as urine cups. After one hour, the water sample remained in the first chamber of the cup and did not enter the second chamber by passing the passage. The lids were then attached to the cups, which pushed down the push rod and moved the plunger from the first position to the second position when the lid was applied.
five minutes was observed that water through the passage
entered the second chamber. The water sample wetted the
Test strips without flooding them. The cup was then in a
placed in a barometric container and one for three minutes
subjected to barometric pressure of 0.7. No leakage was observed.
Example illustrates the use of a device of the invention
for detecting drugs in human urine using
a competitive assay. Thus, a line would be at the
Test line occur if no analyte in the sample would be present.
A test strip was available to detect the following analytes: THC,
Opiates (OPI), PCP and methamphetamine (MET).
of drug-free urine, urine with a -50% cutoff, urine
with a + 50% cutoff, and a threefold cutoff were placed.
Each sample was washed three times using the invention
Device analyzed by placing the urine sample in the beaker
and the lid was attached, allowing the examination (assay)
five minutes, the results were observed. In the drug-free
Urine specimen was on the test line for the three repetitions
all analytes observed a bright line, which is good for everyone
Analytes indicated a negative result. For the sample with -50% cutoff
became one for all three repetitions for all analytes
Line observes what a negative result for all analytes
indicated. The samples with + 50% cutoff occurred in each of the three
Repetitions either no line or a very weak line
on, which indicated a positive result for all samples.
For the tri-cutoff samples, no line was visible
showed a positive result.
Illustratively described herein may be in the absence
any element or elements, restriction
or restrictions that are not specific here
were revealed. The terms and expressions that used
are used for the purposes of description and are not restrictive,
and there is no intention that the use of such terms
and expressions have any equivalents of those shown
and features described or their parts,
However, it is recognized that various modifications within
of the claimed scope of the invention are possible.
Thus, it should be understood that although the present invention
specifically by various embodiments and optional
Features, the skilled person to a modification and modification
can use the concepts disclosed here, and that
such modifications and changes as within the scope
of this invention, as defined by the appended claims
Content of articles, patents and patent applications and all others
Documents and electronically available information,
which are mentioned or cited here are hereby by
Incorporated reference in its entirety to the extent that
as if every single publication is specific and
individually as incorporated by reference.
Applicants reserve the right to choose any and all materials
and information from any such articles, patents, patent applications
or other documents physically incorporated into this application.
QUOTES INCLUDE IN THE DESCRIPTION
The documents listed by the applicant have been automated
generated and is solely for better information
recorded by the reader. The list is not part of the German
Patent or utility model application. The DPMA takes over
no liability for any errors or omissions.
Cited patent literature
- US 4976923 
- - US 5429804 
- US 6726879