DE202021106907U1 - Holding device for a light guide and/or a light guide bundle and device for the quantitative determination of at least one fluorescent, light-absorbing and/or light-reflecting component - Google Patents

Abstract

Holding device for an optical fiber and/or an optical fiber bundle (26) with at least one receiving body (12) divided at least in two, comprising a first and a second partial body (14, 16), the first and the second partial body (14, 16) being arranged such that they can move in relation to one another and can be closed with one another by means of at least one closure device (18), and in the receiving body (12)
- In the first part (14) at least one receiving opening (20) for receiving the light guide and/or light guide bundle (26) or a light guide and/or light guide bundle (26) receiving ferrule (28); and
- in the first and/or second partial body (14, 16) there is at least one recess (22) for receiving an at least partially transparent tube (30) that can be filled with a medium (32) to be analyzed, the receiving opening (20) being designed in this way and arranged to be light-conductively connected to the recess (22).

Description

The present invention relates to a holding device for an optical fiber and/or an optical fiber bundle with at least one receiving body divided into at least two parts, comprising a first and a second partial body, the first and the second partial body being arranged such that they can move with respect to one another and can be closed with one another by means of at least one locking device. The invention further relates to a device for quantitatively determining at least one fluorescent, light-absorbing and/or light-reflecting component.

Such holding devices and measuring devices are known from the prior art. For example, holding devices in the form of a clamp or a clip from the U.S. 9,173,988 B2 known. The clamps are releasably attached in a manner known per se to a transparent tube in which blood flows as the medium to be analyzed. In combination with a light source, signals are generated from the blood in the transparent tube by being illuminated from one side and detecting the transmitted light on the other side. A disadvantage of the known holding devices, however, is their complicated and cost-intensive structure, since here there is always an emitter emitting the light into the tube and a detector arranged opposite the emitter, in particular on an opposite clamp arm. Due to the fact that they are fixed to a specific detector, the known holding devices are also not flexible, ie they cannot be used for different measurements.

The same applies to the known devices for quantitatively determining at least one fluorescent, light-absorbing and/or light-reflecting component, which use such a holding device.

The object of the present invention is therefore to provide a holding device and a device for quantitatively determining at least one fluorescent, light-absorbing and/or light-reflecting component of the type mentioned at the outset, which on the one hand can be produced inexpensively and on the other hand ensures a wide range of applications.

A generic holding device according to the features of claim 1 and a generic device for the quantitative determination of at least one fluorescent, light-absorbing and/or light-reflecting component with the features of claim 20 serve to solve these tasks.

Advantageous configurations with expedient developments of the invention are specified in the respective dependent claims, advantageous configurations of the holding device being to be regarded as advantageous configurations of the device for quantitatively determining at least one fluorescent, light-absorbing and/or light-backscattering component and vice versa.

A first aspect of the invention relates to a holding device for an optical fiber and/or an optical fiber bundle with at least one receiving body divided at least in two, comprising a first and a second partial body, the first and the second partial body being arranged such that they can move with respect to one another and can be closed with one another by means of at least one closure device. At least one receiving opening for receiving the light guide and/or light guide bundle or a ferrule accommodating the light guide and/or the light guide bundle is formed in the first part of the receiving body. The term ferrule is understood to mean a small guide tube which accommodates the light guide or the light guide bundle or the corresponding light guide fibers. Ferrules are usually made from a material with a high degree of hardness and can be made from hard metal, metal, ceramics or plastic, for example.

In addition, at least one recess for receiving an at least partially transparent hose that can be filled with a medium to be analyzed is formed in the first and/or second partial body of the receiving body. Furthermore, the receiving opening is designed and arranged in such a way that it is connected to the recess in a light-conducting manner. On the one hand, the holding device according to the invention can be produced inexpensively and, on the other hand, it ensures a wide range of applications. In contrast to the holding devices known from the prior art, the holding device according to the invention can be constructed more simply. For example, no light sources such as LEDs or photodiodes need to be integrated with their wiring. The structure of the holding device according to the invention means that it only has to hold the light guide and/or the light guide bundle and the tube filled with the medium to be analyzed. Light sources, detectors or the like do not have to be accommodated in the holding device and can therefore be larger and more powerful since they can be designed as external devices. Since only the optical light guide and/or the light guide bundle are arranged in the holding device according to the invention, no power supply, ie the conduction of electrical currents, is necessary within the holding device. this makes possible safe use of the holding device. The present holding device solves the problem of simply, quickly and reproducibly attaching one or more light guides and/or light guide bundles to a hose in order to irradiate the hose and its contents with light, including the excitation of fluorescence of the hose contents and the possibility that backscattered or to direct fluorescent light through the light guide and/or the light guide bundle to a detector or an evaluation device.

In a further advantageous embodiment of the holding device according to the invention, a longitudinal axis of the receiving opening is arranged perpendicular to a longitudinal axis of the recess. This results in a predefined and reproducible position of a light guide or light guide bundle arranged in the receiving opening relative to a hose arranged in the recess and transparent at least in the region of the recess. "Perpendicular to" also means a deviation of +- 10° from the vertical. The receiving opening and/or the recess can have a circular, oval, semicircular or polygonal cross section.

Other cross-sectional shapes are also conceivable. The cross section of the receiving opening and/or the cross section of the recess can be designed in such a way that several light guides or light guide bundles or hoses can be fixed next to one another in the holding device or the receiving body of the holding device.

Furthermore, the recess in the receiving body can be designed to receive the hose with a precise fit. It is therefore not possible for the hose or the receiving body to slip due to its own weight. A precisely fitting enclosure can be achieved, for example, by drilling a cylindrical hole with an outer diameter of the tube in the receiving body and then dividing it centrally or eccentrically along its axis. If the receiving body is divided off-centre, a larger and a smaller notch remain in the first and second partial body of the receiving body. The result of this is that the tube must be slightly deformed when it is inserted until it lies completely in the larger notch of the recess. Subsequently, the first and the second partial body are closed with one another, so that the tube is completely enclosed within the receiving body and within the recess 22 . The recess can be lined at least partially with an elastic and/or flexible material. For example, rubber or foam lips can be used. As a result, the adhesion of the tube within the receiving body can be increased since the elastic and/or flexible material presses against the tube.

In further advantageous configurations of the holding device, the recess is designed to accommodate hoses with different diameters and/or wall thicknesses. For example, the recess can have a diameter that tapers in the direction of the receiving opening. The recess of the receiving body can be shaped in such a way that hoses with different ratios of diameter to wall thickness are held in such a way that their inner surface is positioned at a similar distance from the light guide or light guide bundle arranged in the receiving body. In order to achieve this, the recess can have a diameter that tapers in the direction of the receiving opening, for example in a V shape, with the light guide or the light guide bundle being positioned at the tip of the V-shaped recess. Independence from the hose diameter and/or its wall thickness can also be achieved by using an optical component such as a lens and/or by using the curve of the hose as a “cylindrical lens”.

In further advantageous configurations of the holding device according to the invention, this or the receiving body consists of an opaque material at least in a region around the receiving opening and/or the recess. However, it is also possible for the receiving opening and/or the recess to be lined with an opaque material. The receiving body is thus shielded from ambient light in the area of the receiving opening and the recess, as a result of which the measurement accuracy is significantly increased when the holding device is used as intended.

In further advantageous configurations of the holding device according to the invention, the receiving opening and/or the recess are designed in such a way that in a transition area between the receiving opening and the recess there is an intermediate space for receiving a liquid, a gel or a transparent solid in order to adapt a refractive index between the light guide and/or Light guide bundle and the hose is formed. As a result, specular reflections can advantageously be minimized. Furthermore, there is the possibility that the recess has a fluorescent coating on a side opposite the receiving opening. This predefined fluorescent coating can be used as a calibration standard for Fluorescence measurements are used. Finally, there is the possibility that the recess has a reflective coating on a side opposite the receiving opening. This can be diffuse or reflective. This makes it possible to measure an absorption of the medium to be analyzed in the tube using reflected or scattered transmitted light.

In further advantageous configurations of the holding device according to the invention, the receiving opening is designed to receive the light guide and/or light guide bundle or the ferrule accommodating the light guide and/or the light guide bundle with a precise fit. By inserting the light guide and/or bundle of light guides or the ferrules with a precise fit, it is impossible, for example, for these to perforate the hose. There is also the possibility that a spacer for defined positioning of the light guide and/or light guide bundle or the ferrule accommodating the light guide and/or the light guide bundle relative to the recess or the hose arranged in the recess is formed in the receiving opening. On the one hand, this also serves to prevent the tube from being perforated by the light guide or the light guide bundle or the ferrule. On the other hand, the measurement accuracy is increased by the defined distance when the holding device is used as intended. However, it is also possible for the corresponding end of the light guide and/or light guide bundle or the corresponding ferrule to be arranged in the holding device in such a way that it touches a hose or its peripheral surface which is correspondingly arranged in the holding device. Furthermore, at least one fastening element for defined fastening of the light guide and/or light guide bundle or the ferrule accommodating the light guide and/or the light guide bundle can be formed within the receiving opening in the first partial body of the receiving body of the holding device. Gluing or clamping the light guide, the light guide bundle or the ferrule within the receiving opening is also conceivable and serves to securely fix the elements mentioned together.

In further advantageous configurations of the device according to the invention, the closure device comprises at least two fastening clips arranged at opposite ends of the first and second partial body. However, there is also the possibility that the closure device comprises at least one magnetic closure arranged on the first and second part body. In addition, there is the possibility that the first and second partial bodies of the receiving body can be designed so that they can be opened and closed by means of at least one hinge device. In a further conceivable embodiment, the second part-body in turn comprises two part-bodies, the further part-bodies being able to be closed by means of a further closure device. The closure device can in turn comprise fastening clips, a magnetic closure or a hinge device. Overall, the first and second partial body as well as the receiving body can be embodied as a block. The at least two-part design of the receiving body ensures, in particular, that a hose can be easily inserted or inserted when the holding device is used as intended.

A second aspect of the present invention relates to a device for the quantitative determination of at least one fluorescent, light-absorbing and/or light-reflecting component. The device comprises at least one holding device according to the first aspect of the invention. In addition, the device has at least one optical fiber and/or an optical fiber bundle, which is detachably connected to the holding device at one end, or a ferrule accommodating the optical fiber and/or the optical fiber bundle. The device also comprises at least one light source for generating an excitation light, the light source being connected to the end of the light guide and/or bundle of light guides opposite the holding device in a light-conducting manner. Furthermore, the device comprises at least one evaluation device for receiving and quantitatively evaluating the absorption, backscattering or fluorescence generated by the excitation light in the medium to be analyzed and guided in the tube, the light guide and/or the light guide bundle being designed in such a way that the generated absorbed, backscattered and/or fluorescent light components are fed to the evaluation device through the same light guide or through the same optical fibers or parts of the same light guide bundle that are not supplied with excitation light. The device according to the invention solves the problem of simply, quickly and reproducibly attaching one or more light guides to a tube in order to irradiate the tube and its contents with light, including the excitation of fluorescence of the medium located in the tube, the backscattered or fluorescent Light can be passed through the one or more light guides to the evaluation device. The device according to the invention can be implemented inexpensively and can also be used in a mobile manner. With the device according to the invention, for example, fluorescence measurements can be carried out on a hose or on the contents of a hose. In particular, erythrocyte-bound zinc Protoporphyrin (ZnPP) and/or protoporphyrin IX (PPIX) can be detected in the blood, which can be used to diagnose iron deficiency.

Comparable, known devices generate signals from the blood in a transparent tube by being illuminated from one side and the transmitted light is detected on the other side. In the case of the device according to the invention, the illumination and detection are advantageously carried out from the same side of the hose. This "front face" geometry makes it possible to measure not only the absorption but also the fluorescence, which is not possible or only possible to a very limited extent with known devices. For example, the excitation of porphyrins in the blood requires short-wavelength excitation, which results in a very low penetration depth of the light. Even a single layer of erythrocytes completely absorbs light of these wavelengths. Although the emitted fluorescent light could penetrate a thin layer of blood, a measurement through an unmodified tube for dialysis or blood donation would not be possible because of the large amount of blood between the emission point and the detector in the transmission. Advantageously, the device according to the invention makes it possible to capture the fluorescent light emitted directly on the side on which it is excited, thereby guaranteeing maximum sensitivity. In contrast, the prior art devices have illumination and detection on opposite sides of the tubing.

In further advantageous configurations of the device according to the invention, the medium is blood and the hose is a dialysis hose. Advantageously, when using the device according to the invention, proportions of ZnPP and/or PPIX can be determined from the blood in the dialysis tube during dialysis.

Further features and their advantages can be found in the descriptions of the first aspect of the invention, advantageous configurations of the first aspect of the invention being to be regarded as advantageous configurations of the second aspect of the invention and vice versa.

Further features of the invention result from the claims, the figures and the description of the figures. The features and combinations of features mentioned above in the description and the features and combinations of features mentioned below in the description of the figures and/or shown alone in the figures can be used not only in the combination specified in each case, but also in other combinations without departing from the scope of the invention . The invention is therefore also to be considered to include and disclose embodiments that are not explicitly shown and explained in the figures, but that result from the explained embodiments and can be generated by separate combinations of features. Versions and combinations of features are also to be regarded as disclosed which therefore do not have all the features of an originally formulated independent claim. Furthermore, embodiments and combinations of features, in particular through the embodiments presented above, are to be regarded as disclosed which go beyond or deviate from the combinations of features presented in the back references of the claims.

• 1 eine schematische Darstellung einer erfindungsgemäßen Haltevorrichtung gemäß einer ersten Ausführungsform;
• 2 eine schematische Darstellung einer erfindungsgemäßen Haltevorrichtung gemäß einer zweiten Ausführungsform;
• 3 eine schematische Darstellung einer erfindungsgemäßen Haltevorrichtung gemäß einer dritten Ausführungsform;
• 4 eine schematische Darstellung einer erfindungsgemäßen Haltevorrichtung gemäß einer vierten Ausführungsform; und
• 5 eine schematische Darstellung einer erfindungsgemäßen Vorrichtung zum quantitativen Bestimmen mindestens einer fluoreszierenden, lichtabsorbierenden und/oder lichtrückstreuenden Komponente.

Show it:

• 1 a schematic representation of a holding device according to the invention according to a first embodiment;
• 2 a schematic representation of a holding device according to the invention according to a second embodiment;
• 3 a schematic representation of a holding device according to the invention according to a third embodiment;
• 4 a schematic representation of a holding device according to the invention according to a fourth embodiment; and
• 5 a schematic representation of a device according to the invention for the quantitative determination of at least one fluorescent, light-absorbing and/or light-backscattering component.

The in the 1 - 5 features shown each have the same reference numerals.

1 shows a schematic representation of a holding device 10 for a light guide and/or light guide bundle 26 according to a first embodiment. The holding device 10 in this case comprises a two-part receiving body 12 which consists of a first part-body 14 and a second part-body 16 . The receiving body 12 as a whole, as well as the sub-bodies 14, 16, are of block-like design. In addition, the first and second partial bodies 14 , 16 are arranged such that they can move in relation to one another and can be closed with one another by means of a closure device 18 . In the exemplary embodiment shown, the first and the second partial body 14 , 16 are releasably connected to one another by means of two fastening clips 34 .

It can be seen that a receiving opening 20 for receiving the light guide bundle 26 is formed in the first partial body 14 of the receiving body 12 . The light guide bundle 26 ends in a so-called ferrule 28, the cylindrical housing of which can be inserted into the receiving opening 20 with a precise fit. A fastening screw 24 is also used to fix the ferrule 28 in the receiving opening 20. It can also be seen that a recess 22 for receiving an at least partially transparent tube 30 that can be filled with a medium 32 to be analyzed is formed in the receiving body 12. The recess 22 is formed with a larger notch in the second body part 16 . To arrange the tube 30 within the recess 22, the two-part receiving body 12 is open. The hose 30 is inserted into the notch or partial recess of the recess 22, which is designed to be larger in the exemplary embodiment shown. By closing the receiving body 12 by joining the first and second partial bodies 14, 16, the hose 30 is fixed within the recess 22 and is completely surrounded by the receiving body 12. In the exemplary embodiment shown, the partial bodies 14, 16 are made entirely of an opaque material. As a result, both the section of the light guide bundle 26 located in the receiving opening 20 and the section of the transparent tube 30 located in the recess 22 are sealed off from the ambient light. This increases the quality of the measurements when the holding device 10 is used as intended. The hose 30 shown can be a blood donation or dialysis hose. In addition, it is clear that the receiving opening 20 is designed and arranged in such a way that it is connected to the recess 22 in a light-conducting manner. A longitudinal axis of the receiving opening 20 is arranged perpendicular to a longitudinal axis of the recess 22 . Both the receiving opening 20 and the recess 22 are circular in cross section.

2 shows a schematic representation of a holding device 10 according to a second embodiment. The basic structure of the holding device 10 shown here corresponds to that in 1 illustrated and described holding device. In contrast to the holding device according to the first exemplary embodiment, however, here the recess 22 for the tube 30 is arranged with approximately equal proportions in the first partial body 14 and the second partial body 16 of the receiving body 12 . As in the first exemplary embodiment of the holding device, the receiving opening has a circular cross section. In addition, it can again be seen that the longitudinal axis of the receiving opening 20 is arranged perpendicular to a longitudinal axis of the recess 22 .

3 shows a schematic representation of a holding device 10 according to a third embodiment. In the 3 The illustrated embodiment of the holding device 10 corresponds in principle to that in 2 illustrated embodiment of the holding device. In contrast to the in 2 shown holding device, the holding device 10 according to the third embodiment, however, has a different closure device 18 . It can be seen that opposite magnets 36 are formed in the partial bodies 14, 16 in order to close the first partial body 14 with the second partial body 16 of the receiving body 12, which magnets connect the two halves of the receiving body 12 to one another. A retaining chain 38 is also arranged at one end of the two-part receiving body 12 for loosely connecting the first part-body 14 to the second part-body 16 . This prevents one of the two partial bodies 14, 16 from being lost if they are not connected to one another via the magnetic closure shown.

4 shows a schematic representation of a holding device 10 according to a fourth embodiment. It can be seen that, according to this exemplary embodiment, the first part-body 14 is connected to the second part-body 16 via a hinge device 40 . In addition, the second partial body 16 is divided into two further partial bodies 44, 46, which can be folded open to one another and closed to one another via a magnetic closure 42. In this exemplary embodiment, the recess 22 is formed completely within the second partial body 16 . As in the previously described embodiments according to the 1 until 3 However, the end of the light guide bundle 26 or the ferrule 28 also rests on the surface of the hose 30 here. The receiving opening 20 is in turn designed and arranged in such a way that it is connected to the recess 22 in a light-conducting manner. To accommodate the hose 30, the two partial elements 44, 46 of the second partial body 16 are separated from one another, so that the hose 30 can be inserted into the recess 22. Also in this embodiment - as well as in the 1 until 3 described embodiments of the holding device 10, the elements of the receiving body 12 are made of an opaque material. In addition, it is again clear that the longitudinal axis of the receiving opening 20 is arranged perpendicular to the longitudinal axis of the recess 22 . Furthermore, it can be seen that when the receiving body 12 is in the closed state, the tube 30 , which in turn can be designed as a blood donation or dialysis tube, is pressed against one end of the light guide bundle 26 .

The elements of the receiving body 12 can be made of metal, plastic or another suitable material.

• Methoden zur Bestimmung der ZnPP- und PPIX-Fluoreszenz von Vollblut in einer Küvette sind bekannt. Bei einer bekannten Anwendung gehen Anregungs- und Fluoreszenzlicht als freier Strahl zur/von der Probe. Gegenüber dieser Methode bietet die Verwendung der Haltevorrichtung 10 beziehungsweise der Vorrichtung 60 den Vorteil, dass das zu untersuchende Medium 32 beziehungsweise der Analyt Blut durch den Schlauch 30 fließt, wodurch das Fluorochrom, kontinuierlich ausgetauscht wird und damit die Gefahr des Photobleichens des Analyten während der Messung erheblich verringert wird. Durch entsprechende Untersuchungen wurde bestätigt, dass die Fluoreszenzintensität bei der bekannten Methode mit der Zeit abnimmt, was auf das Photobleichen der ZnPP-Moleküle im angeregten Volumen zurückzuführen ist. Dieser Effekt tritt bei der Verwendung der Haltevorrichtung 10 beziehungsweise der Vorrichtung 60 nicht oder nur in sehr geringen Maßen auf. Aufgrund des kontinuierlich ausgetauschten Blutvolumens und damit der kontinuierlich ausgetauschten Fluorophore bleibt die gemessene Fluoreszenzintensität annähernd konstant. Dies ermöglicht eine längere Messzeit und eine genauere Bestimmung der ZnPP-Fluoreszenzintensität.

5 shows a schematic representation of a device 60 for the quantitative determination of at least one fluorescent, light-absorbing and/or light-backscattering component. The holding device 10 shown here corresponds to that in 1 shown and described holding device 10, so that this to the figure description 1 is referenced. It can be seen that the device 60 additionally has a light source 48 and an evaluation device 50, the light source 48 serving to generate an excitation light and being connected to the end of the light guide bundle 26 opposite the holding device 10 in a light-conducting manner. The evaluation device 50 serves to receive and quantitatively evaluate the absorption, backscattering and/or fluorescence generated by the excitation light in the medium 32 to be analyzed and guided in the tube 30 . In addition, the light guide bundle 26 is designed in such a way that the generated, absorbed, backscattered and/or fluorescent light components are fed to the evaluation device 50 through the parts of the light guide bundle 26 that are not supplied with excitation light. In the following some, non-limiting, application examples of the holding device 10 with the device 60 for the quantitative determination of at least one fluorescent, light-absorbing and/or light-reflecting component and their advantages are described: Advantages through the use of the clip of the utility model compared to other possibilities, the intended ones The purpose of excitation and detection of fluorescent substances flowing through a sufficiently transparent tube is to achieve:

• Methods for determining the ZnPP and PPIX fluorescence of whole blood in a cuvette are known. In one known application, excitation and fluorescence light go to/from the sample as a free beam. Compared to this method, the use of the holding device 10 or the device 60 offers the advantage that the medium to be examined 32 or the analyte blood flows through the tube 30, whereby the fluorochrome is continuously exchanged and thus the risk of photobleaching of the analyte during the measurement is significantly reduced. Appropriate investigations confirmed that the fluorescence intensity in the known method decreases over time, which can be attributed to the photobleaching of the ZnPP molecules in the excited volume. This effect does not occur or occurs only to a very small extent when the holding device 10 or the device 60 is used. Due to the continuously exchanged blood volume and thus the continuously exchanged fluorophores, the measured fluorescence intensity remains approximately constant. This enables a longer measurement time and a more accurate determination of the ZnPP fluorescence intensity.

In a further application, the holding device 10 and the device 60 are used for measuring ZnPP and/or PPIX from the blood in the dialysis tube during dialysis. In another application, it is used to measure ZnPP and/or PPIX from the blood in the tube during blood collection during blood donation. The holding device 10 is arranged on the tube 30 connected to a blood donation bag. The tube 30 is thereby clicked into the recess 22 of the holding device 10 , as a result of which the light guide or the light guide bundle 26 comes into contact with the tube 30 .

QUOTES INCLUDED IN DESCRIPTION

This list of documents cited by the applicant was generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Patent Literature Cited

• US9173988B2 [0002]

Claims (22)

Holding device for an optical fiber and/or an optical fiber bundle (26) with at least one receiving body (12) divided at least in two, comprising a first and a second partial body (14, 16), the first and the second partial body (14, 16) being arranged such that they can move in relation to one another and can be closed with one another by means of at least one closure device (18), and in the receiving body (12) - In the first part (14) at least one receiving opening (20) for receiving the light guide and/or light guide bundle (26) or a light guide and/or light guide bundle (26) receiving ferrule (28); and - in the first and/or second partial body (14, 16) there is at least one recess (22) for receiving an at least partially transparent tube (30) that can be filled with a medium (32) to be analyzed, the receiving opening (20) being designed in this way and arranged to be light-conductively connected to the recess (22). holding device claim 1 , characterized in that a longitudinal axis of the receiving opening (20) is arranged perpendicular to a longitudinal axis of the recess (22). Holding device according to one of the preceding claims, characterized in that the receiving opening (20) and/or the recess (22) have a circular, oval, semicircular or polygonal cross-section. Holding device according to one of the preceding claims, characterized in that the holding device (10) consists of an opaque material at least in a region around the receiving opening (20) and/or the recess (22). Holding device according to one of the preceding claims, characterized in that the receiving opening (20) and/or the recess (22) are lined with an opaque material. Holding device according to one of the preceding claims, characterized in that the recess (22) is designed to receive the hose (30) with a precise fit. Holding device according to one of the preceding claims, characterized in that the recess (22) is at least partially lined with an elastic and/or flexible material. Holding device according to one of the preceding claims, characterized in that the recess (22) is designed to accommodate hoses (30) with different diameters and/or wall thicknesses. holding device claim 8 , characterized in that the recess (22) has a diameter tapering in the direction of the receiving opening (20). Holding device according to one of the preceding claims, characterized in that the receiving opening (20) is designed to receive the light guide and/or light guide bundle (26) or the ferrule (28) receiving the light guide and/or the light guide bundle (26) with a precise fit. Holding device according to one of the preceding claims, characterized in that in the receiving opening (20) a spacer for defined positioning of the light guide and/or light guide bundle (26) or the ferrule (28) receiving the light guide and/or light guide bundle (26) relative to the recess or the hose (30) arranged in the recess (22). Holding device according to one of the preceding claims, characterized in that the closure device (18) comprises at least two fastening clips (34) arranged at opposite ends of the first and second part body (14, 16). Holding device according to one of the preceding claims, characterized in that the closure device (18) comprises at least one magnetic closure (36) arranged on the first and second part body (14, 16). Holding device according to one of the preceding claims, characterized in that the first and second body parts (14, 16) can be opened and closed by means of at least one hinge device (40). Holding device according to one of the preceding claims, characterized in that the second body part (14, 16) comprises two body parts (44, 46), the body parts (44, 46) being closable by means of a further closure device (42). Holding device according to one of the preceding claims, characterized in that the first partial body (14) has at least one fastening element (24) for defined fastening of the light guide and / or light guide bundle (26) or which has the light guide and/or the light guide bundle (26) receiving ferrule (28) within the receiving opening (20). Holding device according to one of the preceding claims, characterized in that the receiving opening (20) and/or the recess (22) are designed in such a way that in a transition area between the receiving opening (20) and the recess (22) there is an intermediate space for receiving a liquid, a gel or a transparent solid for adjusting a refractive index between the light guide and/or light guide bundle (26) and the hose (30). Holding device according to one of the preceding claims, characterized in that the recess (22) has a fluorescent coating on a side opposite the receiving opening (20). Holding device according to one of the preceding claims, characterized in that the recess (22) has a reflective coating on a side opposite the receiving opening (20). Device for the quantitative determination of at least one fluorescent, light-absorbing and/or light-scattering component comprising - At least one holding device (10) according to any one of the preceding claims; - at least one (s) with the holding device (10) detachably connected at one end light guide and / or light guide bundle (26) or the light guide and / or the light guide bundle (26) receiving ferrule (28); - At least one light source (48) for generating an excitation light, the light source (48) being connected to the end of the light guide and/or light guide bundle (26) opposite the holding device (10) in a light-conducting manner; and - At least one evaluation device (50) for receiving and quantitatively evaluating the absorption, backscattering and/or fluorescence generated by the excitation light in the medium (32) to be analyzed and guided in the tube (30), the light guide and/or the light guide bundle ( 26) are designed in such a way that the generated, absorbed, backscattered and/or fluorescent light components are fed to the evaluation device (50) through the same light guide or through the same optical fibers or parts of the same light guide bundle (26) that are not supplied with excitation light. device after claim 20 , characterized in that the medium (32) is blood. device after Claim 21 , characterized in that the hose (30) is a blood donation or dialysis hose.

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