DE20005738U1 - Device for examining the molecular interactions of soluble or suspendable active substances with solid-phase-bound peptide or peptoid target molecules through capillary plates with a large inner surface - Google Patents

Device for examining the molecular interactions of soluble or suspendable active substances with solid-phase-bound peptide or peptoid target molecules through capillary plates with a large inner surface

Info

Publication number
DE20005738U1
DE20005738U1 DE20005738U DE20005738U DE20005738U1 DE 20005738 U1 DE20005738 U1 DE 20005738U1 DE 20005738 U DE20005738 U DE 20005738U DE 20005738 U DE20005738 U DE 20005738U DE 20005738 U1 DE20005738 U1 DE 20005738U1
Authority
DE
Germany
Prior art keywords
target molecules
capillary
capillaries
capillary plate
plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE20005738U
Other languages
German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Forschungszentrum Borstel Leibniz Lungenzentrum FZB
Original Assignee
Laser und Medizin Technologie GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laser und Medizin Technologie GmbH filed Critical Laser und Medizin Technologie GmbH
Priority to DE20005738U priority Critical patent/DE20005738U1/en
Publication of DE20005738U1 publication Critical patent/DE20005738U1/en
Priority to US10/239,986 priority patent/US7431685B2/en
Priority to EP01927823A priority patent/EP1296756B1/en
Priority to PCT/EP2001/003530 priority patent/WO2001072412A1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/045—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers using devices to improve synthesis, e.g. reactors, special vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00511—Walls of reactor vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00513—Essentially linear supports
    • B01J2219/0052—Essentially linear supports in the shape of elongated tubes
    • B01J2219/00522—Essentially linear supports in the shape of elongated tubes in a multiple parallel arrangement
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    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
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    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
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Description

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßtThe description text was not recorded electronically

Claims (31)

1. Vorrichtung zur Untersuchung der molekularen Wechselwirkung von löslichen oder suspendierbaren Wirkstoffen mit festphasen-gebundenen peptidischen oder peptoiden Zielmolekülen dadurch gekennzeichnet, daß die dafür notwendige Oberfläche innerhalb einer Platte in Form von Kapillaren geschaffen wird. Die Kapillaren von einer Plattenseite zur gegenüberliegenden Seite durchgehen, dabei aber untereinander nicht verbunden sind.1. Device for examining the molecular interaction of soluble or suspendable active substances with solid-phase-bound peptide or peptoid target molecules, characterized in that the surface required for this is created within a plate in the form of capillaries. Pass through the capillaries from one side of the plate to the opposite side, but are not connected to each other. 2. Vorrichtung nach 1 dadurch gekennzeichnet, daß eine Vielzahl von parallelen Kapillaren in der Platte angeordnet sind, die Flüssigkeiten durch Kapillarkraft in die Kapillaren ziehen und darin halten.2. Device according to 1, characterized in that a plurality of parallel Capillaries are arranged in the plate, the liquids by capillary force in the Pull and hold capillaries. 3. Vorrichtung nach 1 dadurch gekennzeichnet, daß die Kapillarplatte aus einem der Materialien Glas, Quarz, Silizium, Kunststoff, Halbmetall, Keramik, Metall oder einer Kombination dieser Materialien hergestellt ist.3. Device according to 1, characterized in that the capillary plate from one of the materials glass, quartz, silicon, plastic, semi-metal, ceramic, metal or a combination of these materials. 4. Vorrichtung nach 1 dadurch gekennzeichnet, daß die innere Oberfläche zur kovalenten Bindung von Zielmolekülen befähigt wird oder eine Synthese der Zielmolekülen erfolgt.4. The device according to 1, characterized in that the inner surface for covalent binding of target molecules is enabled or a synthesis of Target molecules take place. 5. Vorrichtung nach 4 dadurch gekennzeichnet, daß die innere Oberfläche durch eine Organosilanschicht, die funktionelle Gruppen zur Verankerung von peptidischen oder peptoiden Zielmolekülen aufweist, bedeckt ist.5. The device according to 4, characterized in that the inner surface an organosilane layer, the functional groups for anchoring peptidic or has peptoid target molecules is covered. 6. Vorrichtung nach 4 dadurch gekennzeichnet, daß zur Silanisierung γ- Aminopropyltrialkoxysilan oder andere organofunktionelle Silane verwendet werden.6. The device according to 4, characterized in that for silanization γ- Aminopropyltrialkoxysilan or other organofunctional silanes can be used. 7. Vorrichtung nach 4 dadurch gekennzeichnet, daß bei Verwendung von Metall- oder Halbmetallkapillarplatten vor der Silanisierung eine Oxidschicht zur Bindung des Silans mittels Oberflächenoxidation der Kapillarplatte geschaffen wird.7. The device according to 4, characterized in that when using metal or semi-metal capillary plates prior to silanization an oxide layer for binding of the silane is created by surface oxidation of the capillary plate. 8. Vorrichtung nach einem der Ansprüche aus 4 bis 7 dadurch gekennzeichnet, daß in der Kapillarplatte örtlich begrenzte Probenareale angelegt werden.8. Device according to one of claims 4 to 7, characterized in that that locally limited sample areas are created in the capillary plate. 9. Vorrichtung nach einem der Ansprüche aus 4 bis 7 dadurch gekennzeichnet, daß eine Vielzahl von Probenarealen jeweils mit einem Durchmesser von bis zu 2.000 µm oder einer Anzahl von bis zu 4.000 benachbarten Kapillaren auf einer Kapillarplatte angelegt werden.9. Device according to one of claims 4 to 7, characterized in that that a large number of sample areas each with a diameter of up to 2,000 µm or a number of up to 4,000 adjacent capillaries on one Capillary plate. 10. Vorrichtung nach 8 oder 9 dadurch gekennzeichnet, daß die Kapillaren eine Länge sowie die Kapillarplatte eine Dicke von 100 bis 2.000 µm besitzen. 10. The device according to 8 or 9, characterized in that the capillaries Length and the capillary plate have a thickness of 100 to 2,000 microns.   11. Vorrichtung nach einem der vorigen Ansprüche dadurch gekennzeichnet, daß durch Aufpipettieren einer Substanz auf die Kapillarplatte die funktionelle Gruppe der Organosilanschicht temporär geschützt wird. Der so geschützte Bereich stellt ein Probenareal dar.11. Device according to one of the preceding claims, characterized in that the functional group by pipetting a substance onto the capillary plate the organosilane layer is temporarily protected. The area thus protected adjusts Sample area. 12. Vorrichtung nach einem der vorigen Ansprüche dadurch gekennzeichnet, daß durch Aufpipettieren eines Reagenz auf die Kapillarplatte ein geschütztes Ankermolekül an jeder Bindungsstelle des Probenareals entsteht, wobei das Ankermolekül gleichzeitig als Abstandshalter zur inneren Oberfläche dient.12. Device according to one of the preceding claims, characterized in that protected by pipetting a reagent onto the capillary plate Anchor molecule is formed at every binding site in the sample area, whereby the Anchor molecule also serves as a spacer from the inner surface. 13. Vorrichtung nach einem der vorigen Ansprüche dadurch gekennzeichnet, daß durch Aufpipettieren von Fluorenylmethoxycarbonyl (FMOC)-geschützter α-Amino­ poly(ethylenglycol)-ω-propionsäure-N-hydroxybenzotriazolester ein geschütztes Probenareal angelegt wird.13. Device according to one of the preceding claims, characterized in that by pipetting on fluorenylmethoxycarbonyl (FMOC) -protected α-amino poly (ethylene glycol) -ω-propionic acid-N-hydroxybenzotriazole ester a protected Sample area is created. 14. Vorrichtung nach einem der Ansprüche aus 11 bis 13 dadurch gekennzeichnet, daß alle nicht geschützten Bereiche der Kapillarplatte chemisch abgesättigt werden.14. Device according to one of claims from 11 to 13, characterized in that that all unprotected areas of the capillary plate are chemically saturated. 15. Vorrichtung nach 14 dadurch gekennzeichnet, daß die Schutzgruppen in den Probenarealen der gesamten Kapillarplatte entfernt werden.15. The apparatus according to 14, characterized in that the protective groups in the Sample areas of the entire capillary plate are removed. 16. Vorrichtung nach 15 dadurch gekennzeichnet, daß peptide oder peptoide Zielmoleküle ortsabhängig sequentiell aus Aminosäuren in den Probenarealen der Kapillarplatte synthetisiert werden.16. The apparatus according to 15, characterized in that peptides or peptoids Target molecules sequentially, depending on their location, from amino acids in the sample areas of the Capillary plate can be synthesized. 17. Vorrichtung nach 15 dadurch gekennzeichnet, daß die vollständigen peptiden oder peptoiden Zielmoleküle ortsabhängig in den Probenarealen der Kapillarplatte gebunden werden.17. The device according to 15, characterized in that the complete peptides or peptoid target molecules depending on the location in the sample areas of the capillary plate be bound. 18. Vorrichtung nach 16 oder 17 dadurch gekennzeichnet, daß durch die ortsabhängig unterschiedlichen Sequenzen von peptiden oder peptoiden Zielmolekülen eine Substanzbibliothek aufgebaut wird.18. The device according to 16 or 17, characterized in that by the location-dependent different sequences of peptides or peptoids Target molecules build a substance library. 19. Vorrichtung nach 18 dadurch gekennzeichnet, daß auf mindestens einer Seite der Kapillarplatte ein mit einem Ventil versehenes geschlossenes und variables Volumen existiert, in das Flüssigkeiten mit löslichen oder suspendierbaren Wirkstoffen sowie andere Flüssigkeiten und Gase eingelassen werden können und abgesaugt werden können.19. The apparatus according to 18, characterized in that on at least one side the capillary plate has a closed and variable valve Volume exists in which liquids with soluble or suspendable Active substances as well as other liquids and gases can be taken in and can be suctioned off. 20. Vorrichtung nach 18 dadurch gekennzeichnet, daß durch Ankopplung von Ultraschall an die Kapillarplatte Gasblasen gelöst werden können.20. The apparatus according to 18, characterized in that by coupling Ultrasound on the capillary gas bubbles can be solved. 21. Vorrichtung nach 18 dadurch gekennzeichnet, daß durch Ankopplung von Ultraschall die Reaktionsgeschwindigkeiten in den Kapillaren erhöht werden. 21. The apparatus according to 18, characterized in that by coupling Ultrasound the reaction rates in the capillaries are increased.   22. Vorrichtung nach einem der vorher genannten Ansprüche dadurch gekennzeichnet, daß die Bindung der Wirkstoffe an die Zielmoleküle nachgewiesen wird.22. Device according to one of the preceding claims characterized in that the binding of the active ingredients to the target molecules is proven. 23. Vorrichtung nach einem der vorher genannten Ansprüche dadurch gekennzeichnet, daß eine Veränderung der Zielmoleküle durch die Wirkstoffe bewirkt und nachgewiesen werden kann.23. Device according to one of the preceding claims characterized in that a change in the target molecules by the active ingredients can be effected and demonstrated. 24. Vorrichtung nach 22 oder 23 dadurch gekennzeichnet, daß die Kapillarplatte transparent oder opaque ist und einer optischen Messung frei zugänglich ist.24. The device according to 22 or 23, characterized in that the capillary plate is transparent or opaque and is freely accessible for optical measurement. 25. Vorrichtung nach 24 dadurch gekennzeichnet, daß lösliche oder suspendierbare Wirkstoffe fluoreszenzmarkiert sind und in die Kapillaren eingebracht werden.25. The device according to 24, characterized in that soluble or suspendable Active substances are fluorescence-labeled and are introduced into the capillaries. 26. Vorrichtung nach 25 dadurch gekennzeichnet, daß nach einer bestimmten Zeit die nichtgebunden Wirkstoffe entfernt werden und die gebundenen Wirkstoffe anhand der Fluoreszenzmarkierung nachgewiesen werden.26. The device according to 25, characterized in that after a certain time the unbound active substances are removed and the bound active substances can be detected using the fluorescent label. 27. Vorrichtung nach 24 dadurch gekennzeichnet, daß die festphasen-gebundenen peptidischen oder peptoiden Zielmoleküle fluoreszenzmarkiert sind.27. The device according to 24, characterized in that the solid-phase bound peptide or peptoid target molecules are fluorescently labeled. 28. Vorrichtung nach 27 dadurch gekennzeichnet, daß lösliche oder suspendierbare Wirkstoffe, Säuren oder andere Flüssigkeiten in die Kapillaren gespült werden, nach einer bestimmten Zeit wieder ausgespült werden und das Vorhandensein der noch gebundenen fluoreszenzmarkierten Zielmoleküle nachgewiesen wird.28. The device according to 27, characterized in that soluble or suspendable Active ingredients, acids or other liquids are flushed into the capillaries after be flushed out again at a certain time and the presence of the still bound fluorescence-labeled target molecules is detected. 29. Vorrichtung nach 26 oder 28 dadurch gekennzeichnet, daß die Fluoreszenzmarkierung mit Licht im UV, im Sichtbaren oder nahen Infrarot angeregt und detektiert wird.29. Device according to 26 or 28, characterized in that the Fluorescence labeling stimulated with light in UV, in visible or near infrared and is detected. 30. Vorrichtung nach 29 dadurch gekennzeichnet, daß die Fluoreszenzanregung mit einem gepulsten Diodenlaser mit einer Pulsdauer von weniger als 10 ns geschieht.30. The device according to 29, characterized in that the fluorescence excitation with a pulsed diode laser with a pulse duration of less than 10 ns happens. 31. Vorrichtung nach 30 dadurch gekennzeichnet, daß der Nachweis der Fluoreszenz zur Unterdrückung von Streulicht in einem Zeittor nach Abklingen des Anregungspulses geschieht.31. The device according to 30, characterized in that the detection of Fluorescence to suppress stray light in a time gate after the decay Excitation pulse happens.
DE20005738U 2000-03-28 2000-03-28 Device for examining the molecular interactions of soluble or suspendable active substances with solid-phase-bound peptide or peptoid target molecules through capillary plates with a large inner surface Expired - Lifetime DE20005738U1 (en)

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DE20005738U DE20005738U1 (en) 2000-03-28 2000-03-28 Device for examining the molecular interactions of soluble or suspendable active substances with solid-phase-bound peptide or peptoid target molecules through capillary plates with a large inner surface
US10/239,986 US7431685B2 (en) 2000-03-28 2001-03-28 Method and device for investigating substance libraries
EP01927823A EP1296756B1 (en) 2000-03-28 2001-03-28 Method and device for investigating the molecular interaction of soluble or suspendable active substances with solid-phase bonded peptidic or peptoid target molecules by means of capillary plates having a large inner surface
PCT/EP2001/003530 WO2001072412A1 (en) 2000-03-28 2001-03-28 Method and device for investigating substance libraries

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10040857A1 (en) * 2000-08-11 2002-02-28 Jens P Fuerste Nucleic acid library or protein or peptide library
EP1226871A2 (en) * 2001-01-24 2002-07-31 Ebara Corporation Reactive probe chip and reactive detection sytem

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10040857A1 (en) * 2000-08-11 2002-02-28 Jens P Fuerste Nucleic acid library or protein or peptide library
EP1226871A2 (en) * 2001-01-24 2002-07-31 Ebara Corporation Reactive probe chip and reactive detection sytem
EP1226871A3 (en) * 2001-01-24 2004-01-21 Ebara Corporation Reactive probe chip and reactive detection sytem

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