DE1806196C - Device for separating a liquid sample into a light and a heavy phase and for sealing the separated phases against one another - Google Patents

Description

2. Apparatus according to claim 1, characterized in that 20 to 30 ° / "of the relevant sub-marked that the piston (46; 64; 148) at search laboratories of the USA unsatisfactory the stopper (48; 60; 146) under the action of a measurement Take blood serum samples,
certain force is releasably attached. 25 With the conventional method, the one

3. Device according to claim 2, characterized in that the blood sample taken from the patient is placed in a collection, that the detachable attachment of the vessel is filled. The collecting vessel then rests for about a Piston (46; 64; 148) on the stopper (48; 60; half an hour; during this time a partial 146) is designed in such a way that during the phase separation of the blood separation which forms the heavier phase The centrifugal force that occurs is to hold cells from the blood forming the lighter phase able, however, to educate with a short-term serum. For complete separation, the Increasing the centrifugal force is resolved. The sample is then placed in a centrifuge. There-

4. Apparatus according to claim 2 or 3, after which the separated serum is analyzed. Election by characterized in that the releasable fastening method can anticoagulate the blood sample movement of the piston (46; 64; 148) to the stopper 35 are added, which then without prior Gravi- (48; 60; 146) is designed so that it can be centrifuged to separate the force. To this one through the stopper (48; 60; 146) and the manner in which a separation into a cell phase is achieved, Piston (46; 64; 148) in the container (40) one which forms the heavy phase, and in a light one able to withstand urgent hollow needle. Phase of blood plasma. The plasma can then be used for

5. Apparatus according to claim 2, characterized in that 40 test purposes are used.

characterized in that the stopper (146) and the upon analysis of the blood serum or blood plasma

Piston (148) made in one piece from a rubber elastic- the test methods can be used for an investigation

see are shaped and over a material while determining the percentages of an or

the action of a certain force destructive include several of the following components: GIu-

connecting link (150) connected to each other, cholesterol, calcium, inorganic phosphorus,

den are that the piston (148) is one of the bilirubin, albumin, protein, uric acid, urinary stick-

Inner wall of the container tightly fitting substance, lactic acid dehydrogenase, alkaline phosphate

Disc (152) on which the stopper (146) draws tase, protein-bound iodine, serum glutamine / oxaline

turned side and that the remainder has acid transaminase and other components. There

of the piston (148) smaller radial dimensions and each of these components is generally a direct one

gen than the disc (152) has. Relationship to physical condition and health

6. Device according to one of claims 1 indicates the examined person is natural to 5, characterized in that the specific highest accuracy is essential.

see weight of the piston (46; 64; 148) between An important prerequisite is compliance that of the light and that of the heavy phase 55 absolutely sterile conditions. With most of them Conventional procedures require that the

Serum or plasma after separation from the

Collection vessel transferred to another container

will. There are several ways the plasma

The invention relates to a device for separating 00 or serum in a conventional manner from the first

tion of a liquid sample, especially blood, to be removed in a container. Often this happens through

a light and a heavy phase by sediment decanting, aspiration or by use

tation or centrifugation and for sealing a vacuum needle. This is twofold

separate phases from each other, consisting of dangerous: the serum or plasma becomes unsterile

exposed to a pipe that can be closed by a plug, and besides that, there is

shaped as a centrifuge tube risk that the second container is not perfect

Sample container as well as one on the inside is sterile.

against the wall of the sample container, a further danger arises with such

3 4

drive out of the need to sample during the It has been suggested a variety * -} *} * "

to identify the entire clinical process. One small plastic particles having special emneitucner will be appreciated that the danger of incorrect labeling size, shape and specific gravity to ^v * ™ ^en ~ voltage or the loss of the true identity of the donations to provide a barrier between serum * ^ ¹ ^ ^or * This is always the case when the serum or plasma 5 and the cells erect. With this Nietnoae from the collecting container or tube into another, the loose particles are transferred before the phase separation vessel. put in the blood sample, and the tube becomes one

Transferring serum or plasma involves exposure to normal centrifugal separating force. the further obvious danger of spillage in plastic particles tend to be in a desich, whereby the laboratory staff with blood at rest is to arrange the correct position inside the tube tion could come, which under certain circumstances healthy and a separating layer between the Seruni or Contains harmful substances. Often contain plasma and cells to form. This bystem However, such blood samples, which are hepatic or syphilitic, do not permit subsequent irrigation Serum, which is the coming in contact or extraordinary movement because this for /.er-Pcrsonal could infect. 15 disruption of the barrier. Hence who exists

One of the main problems with these, the danger that an unaccustomed movement will interfere with the interrogation, is the fact that in the case of the awake separating layer, the number of such tests is unpredictably destroyed and the number of such tests increases in time. leads to inaccurate readings chemical, door aie khe delay between sampling the person who does not have to Laboreinnchtungen uri ">: ii.nten and performing the analysis occurs" o and feature point to the need for results in m-kanntlich can this delay Very dangerous transport of the samples either through the rust λ earth and inaccurate results with regard to or through other means of transport. The formed:, ozentgehattes of the constituents contained in the blood separating layer would be the cause of such an iranspori ... It is Z. B. known that the glucose occurring shocks not withstand. The invention is based on the object of leaving a blood serum and cells (or plasma and cells) device of the type mentioned at the beginning to stand. This enables a reliable phase separation of the blood of a diabetic to be undermined, which results in a satisfactory result even in the case of subsequent auditory test results after the separation automatically against reality the sugar content is undesirable or even sealed off from each other, so that each is mechanically high. In addition, the enzymes of the visual or chemical interaction between the lactic acid dehydrogenase and the glutamine-oxal phases are prevented.

siiiretransaminase as the time elapses from the invention, this task is done by gezellen and pass into the serum. This hits 35 triggers that the piston from a rubber elastic _<; i! ch too for the potassium cation. In addition, it is made of material and under the action of an important that the carbon dioxide and blood pH values acting in the longitudinal direction of the sample container are kept constant. fugalkraft from the sample liquid radially deformable

In the interest of getting accurate test results. and can be flown around.

which are often crucial for correct treatment, 40 The sample container can also serve as a sample collection such inaccuracy will not be tolerated. Uncovering the transfer to a second container the. It should be noted that such. The elastic piston seals against the internal decelerations Not only in hospitals, the wall of the tubular container goes down and can go down increased demands on your laboratory equipment due to the serum or plasma phase of the biuie ask, but also move to doctors and clinics without 45, if the appropriate Kratt aur inn these bodies occur. The last group is being exercised. At the same time, the plasma can ooer Remember to take appropriate precautions for the flow of serum upward around the flask. of samples to be taken to laboratories. When the piston is up to the interface between

Through the USA patent specification 1,066,164 a plasma or serum and cells have moved downwards, device of the type mentioned is known- 50 the force effect is reduced whereby the one in which the piston is sealed by a solid piston between the two phases mechanical link is moved downwards. forms. o _n A " _n

Since the tube used there goes down over the Preferably, the piston is releasable at the bottom

Piston crown protrudes, additional front of the plug must be attached. The attachment can by means of Precautions must be taken to secure the point 55 by means of a special fastening device place on which the downward movement of the piston of an adhesive, by means of an integrally molded should be stopped. Another disadvantage of this link can be made or also Device can be seen in the fact that none take place automatically by frictional interlocking Acting seal between the to be separated According to a preferred embodiment of the

Phases is provided, whereby the phases according to the 60 invention is the releasable attachment of the piston to Centrifugation mechanically and chemically separated the stopper so that they can be used in phases can withstand separation occurring centrifugal force

It is true that the devices that have been customary up to now can, but with a brief increase in the blood sample, relieve a person without the risk of the centrifugal force of infection. It is advisable to remove it, but the subsequent 6 ₅ suggest that the detachable attachment of the scoop at the end of the separation of the serum or plasma from the stopper is designed in such a way that it can cope with the force of cells and the removal of the serum or Pias the stopper and plunger into the container to analyze the difficulties mentioned above. able to withstand urgent hollow needle.

5 6

Furthermore, it can be provided that the stop undergoes any unusual treatment up to almost the furnace and the piston undergoes one-piece? From a rubber - destruction of the container itself,
elastic material are formed and over a under The invention is described by the following

the action of a certain force destructible exercise of the execution intermediate member shown in the drawing are connected to each other that the 5 examples explained in more detail. In the drawing shows
Piston one on the inner wall of the container F i g. 1 a conventional arrangement for sterile

Sealed disc on the stopper receiving a blood sample from a patient facing side and that the remaining part has been removed,

of the piston smaller radial dimensions than the F i g. 2 an arrangement with a rubber glass

Disc owns. io see flask, which is above that of the cells

Another embodiment of the invention is arranged in separate plasmas or serums,
3, the arrangement shown in FIG. 2, after the piston lies between that of the light phase and that of the piston, part of its path through that of the heavy phase. This brings the blood serum or plasma back,
Piston automatically at 15 F i g on its downward movement. 4 stops the piston in its final seal in the correct place. position above the surface of the cell phase,

The device according to the invention thus represents a fig. 5 and 5 A a preferred embodiment

effective and economical means of removal, the plug-piston assembly,
Phase separation and sealing of the corresponding FIG. 6 another expedient embodiment

Blood phases against each other, all in a single form of the stopper-piston assembly.
The invention also enables Fig. 1 shows a sterile blood collection arrangement

automatic sealing using conventional art. This arrangement consists of an increased centrifugal force immediately after a manifold 2, which protrudes through a the separation of the two phases by centrifugation. Plug 4 is sealed. The sealed collection die detachable fastening of the sealing piston as tube ?, is inserted into a cup-shaped vessel 6, At the bottom of the cup-shaped vessel 6 is a guaranteed to the stopper from the start Sterility of the container and finally subdivided it holder 8 arranged for attaching a hollow two departments with two completely separate needle 10 is used. One end of the needle 12 is located separate blood phases, with the one on top outside the cup-shaped vessel, while the Stopper the inside of the container from impurities 30 other end 14 of the needle protrudes into the vessel, protects by the outside atmosphere. When taking a blood sample, the needle

Since it is not necessary to introduce the blood from the end 12 of the patient, to refill the other needle collection container and to push the second container tip 14 through the stopper 4 and thus mark it for identification purposes, it is a connection to the interior of the collection tube 2 completely excluded that the sterility of the sample 35 established. Since the sealed collecting tube and the identity of the donor are lost or evacuated, the patient's blood flows through the hollow needle into the collecting tube 2, which is dangerous for the laboratory staff. Furthermore, there is the risk of chemical contamination, which will affect the test results, and the needle tip 12 will be withdrawn and the pot will flow. and the loss of sterility due to a negligent 40 or cup-shaped vessel 6 from the collecting tube 2 by removing the cleaning of reusable glass parts. The needle end 14 is thereby switched back. F.in economic advantage also results and the connection with the interior of the insofar as there are no two independent glass parts of the manifold 2 is interrupted. The elasticity needed for each blood sample taken. Since that of the stopper 4 ensures that the opening is perfectly separated by the two phases and that 45, which the needle end 14 passes through, no chemical interaction takes place again, one is closed. Hence, this system provides sterile, indefinite storage with no risk of inaccurately sealed tube containing a blood sample.
Display results regarding the proportion given depending on whether one is blood serum or blood plasma

desires to obtain chemical constituents in the serum or blood then becomes one of the two guaranteed. Shipping to a location other than 50 following procedures applied. If blood serurr where the sample was taken is also desirable. the closed tube remains about 2 ( tert, if you do not stand by such a 40 minutes to a partial Trennunj Treatment and delay to cause any inconvenience. To complete the separation in two inaccuracies must be expected. Should it be necessary to phase blood, the tube is then placed in a centrifuge the same sample was introduced to test the initial 55. At the bottom of the pipe there is dii To test the results again, there must also be a heavy phase consisting of the squeezing unlimited storage in this container possible th blood cells, while the light phase since being. This object is achieved by means of the invention. Serum, located at the top of the tube.

It can also be seen that the application of the In the interest of simpler description stel

Invention no retraining of the medical teacher 60 deT expression "easy phase" for the part de sonals, be it for blood collection, low density fluid, and expression be it with regard to the treatment and analysis of the ent- "heavy phase" for the high part of the liquid usually blood. Eventually the instant density switches off.

and permanent sealing by the other hand, if one isolates plasma ζυ

pressed elastic part wants the risk of chemical 65, an anti-coagulant must be in the RoI Interaction and the slow exchange between are given to prevent blood clot formation 2 the phases also prevent the container from normal. This tube can then be centrifuged immediately Tfaii'-po'ih claims is exposed or yawed without first starting out

SSt fs!

In the case of the in Fi g. 2 to 4 8 «^^ A ^ uhruigrfom *" £ ™ Bäumen \ f * '^ therefore contains a tubular sample container 40 eme au 5 J * rm £ g J ^ _{42 44 the} heavy phase = existing carefully against blood cells 42 and1 a _abgedicn of two ^ ^^ _impurity blood plasma or Serurn existing light Pha * 44th ema gg ^ ^ _indefinitely an elastic rubber piston 46 undemelasucher tet harmful chemical change Sf 48 preceding ind "The d" t "lj accounts ^ g ^ _{ph D BhäUer}

A rubber elastic piston 46 undeu harmful chemical

Plugs 48 are in front of the d «t« lj accounts ^ g ^ ^ ^. ^ _{Phases Def}

46 has a diameter larger than the inner ^ ^ ^ _other ^ ^

d Bhlts 40 '''J"Jj ^dai ^ J _j ie danger of pollution or

The th position, i.e. inserted into the container, is the _{result of the} accuracy of the result

Piston 46 ^^ Γ ^^ Γ ^ ηΤβ has an extensive clinical analysis of the blood sample as

the container 40 from. De ι-stopper 48! Has «nen erje, ψ ^^ Transportes.

terten part or head 50 "^ * ¹¹ * ¹ ^ ₁₁ ¹ JSSSE Except for a hollow needle for taking blood samples

d, the S r when inserting topfens in R ^"hr ^ ~ _{in a person becoming} en frequent small amounts of blood

ter 40 serves as a stop. The tamper, 48 has terner ^ ^. ^ ^ _{Fjnger of a pers (m}

a lower part 52 lower ^ By measuring the it Lasn _{to dner} ^ _{ldcht to.} Tubular container 40 inserted * ™ ^d ™ «Aeg ^ ₂₀ g ^ same place removed. That for such de-

Condition appropriately used equipment is generally a

The plug 48 is provided with an ^{ax 'a]} f "^ Srieten elongated tubular container with a vented stop

Vcrschcn for reasons described below ^ 8 ^^. ^ _{and the opening of othercits} . _The

are. . The open end arrangement is against the puncture in the person

With the preferred _{application of the arrangement back and through clear} seepage, this will be the case

according to FIG. 2 to 4 ^ειη ^ ^ε "^ '^^^ _Γ5 ϊη blood is drawn into the tube. The ventilation ensures that

with the stopper 48 and the piston J6 jersew ^ _{escape of the air> when} the blood enters the tube

which are arranged according to Fig. 5 * ^nd ^ ^s f ⁿ " ^Blu \ _b ^ ^^ ^ ^ _Rö ^ ^ _{dgnn by}

the tubular container g « ^a ™ ^meI ' ^w * ^r ° ^ ₅ .:" _Fi - ι a cap tightly closed while the ventilated

would take the stopper 48 with the means the mrii _fen ^ '^ _{on the} other end of the tube removed

shown are The AusnehinungM ^ cjn ™ ^ ^ J ^ ^^ ^ ^ _{Stuff you hlo sen}

Plug thickness through which the Na del mnau g ^ ^ ^ ^ _{Einfül, clothes dcs B} j _{ules, "the}

: m.ß. to deal with the inside of the; Bena crs tube containers and the amount of blood drawn

Avoidance to kick. After Λ JJ «P * e ™ ^ _{sjnd this procedure is} different, but is

sealed container 40e ^ ⁿ S «'J; _{with or 35 the} arrangement according to the present invention

Centrifugal separation sample ^under ^ f "l""can also be used for such processes.

Onne previous deposition period _Wieooen ^ fc indicated, the piston 46 is in the

precisely, it is important that ^"r ^ ^ ™ ⁶ is arranged. ^} ™ V _ne container below the plug. In the

Interaction of the two ^blood ^ "' ^O _{men is} . Preferred embodiment of the subject-matter of the invention-

Sealing made between the phases «iro. ^ was ^ _{the piston 46 releasably attached to the plug} 48

S -.! Soon the separation took place ^η » ^χ . ^ _{Although dej} . _{Pistons also have} a separate one

F i c 2 shows the arrangement «^ '^ f ^ S ^ pin unit and not be attached to the plug 48,

• Entrifugal separation of the phases ^ ¹ ^ ¹¹ ' ¹ ' ¹ "^" ^ _but a better control of the piston movement is

, is then increased to such an extent that the noi _{ynd dje handling of stopper} f _{en 4} g _and col-

, moved forward with simultaneous movement of the KW ^^ ^ ^ ^^ _{through the connection of the [}

* ^o the _{flask at} parts facilitated in Phase 44 to the descending ^K ° '^ J together. At the in

and generally upwards if »" - " _{y F} ■ _{2 to 4} illustrated embodiment can be the

has moved downwards. up to the point where '** ™ ^U1 _Ko f _{ben 46 at St fcn 48 with} means of a in F i g. 2

! Laugh the surface of the ZeI ^] P ^ ^se f ^^ ü- excessively thick adhesive 56 shown to be attached.

the centrifugal force ended, the AWM _alterna tiv can the piston 46 by separate

supply to end of the piston 46 and it cut down ^ ^ _{plug 48 angcbrachl de}

to bend into a sealing position for separation * _ embodiment according to Fig.

of cells 42 from the serum ° ^de ™ Nahrungsmittel ** ^ and 5 A are plunger 146 and stopper 141

the in Fi g. 3 has the ^K ™ ^en , ^ _{ciner cinst} ückigen unit by means of a mistake

Part of its way through the serum '« ^d ^ ^r ™ ^a _{r 55 dungsstabc} s 150 connected. This rod 150 sol

and Fig. 4 shows the piston _{tearing off in its predetermined} force, and

final sealing position. _{n the piston l46 for} the downward movement into the

From Fig. 4 it can be seen that the " ^c " ^a ' _{2 tube} container is released.

its sealed lower division * cä iict ^. ^ In the _{embodiment shown in Fig. 6}

receives and in its _c sealed en upper . *> _{the plug} 60 has an axial recess in

development, the plasma or Serurn 44. Since the "JJ ¹ ^ plug ground. The piston 64 has a high thigh

during the whole journey in dismantling P ^ ^ ^ _{def obcrcn ob} ^ _{ä h (}

persists, remains serum or riasma 44 as well as aj ^ ^^ _after ^ _{crslreckl Dcr Rn f 46} ^ _n

cells 42 and against each other sterile abgeai cnici ^ ^ ^ _{AusnehmUTlg 62 cin} g _{cführt un} d stone so emj

During the whole process of the ZeinninMaer _ciiten ^ ^^ _application ^^ stuffing 60 and KoI

would take up to the point in time of the ° ™ '· _{dcT ben} 64.

Separation, no '' ™ ^fulIun * * LgSJhilter urn for any type of attachment between pistons

Ξ SSÄlSiüSS ÄSlon stopper should be made in case prior to the filling of the ^ blood

the sample is introduced into the tube reservoir, the gas pressure that forms opens and the gas

must allow the connection between the piston and the stop step. One can see that this too

Must be strong enough to give a solution a reduced resistance after additional gas discharge

To resist introduction of the needle, through which the downwardly moving piston 148 up to the

Blood sample is filled into the tube container. 5 sealing causes. If desired, one

Even if the filled sample is used to separate the valve for the passage of the light phase into the

If two blood phases are centrifuged, the connecting piston 148 should incorporate the passage 82

dung under the action of the initial centrifugal force through the whole flask and the valve

be able to withstand a solution. can be placed in it.

Then, preferably after exercising a centrifugal force, the material of the piston should in general

fugal force to separate the blood in two phases of sufficient elasticity, which is due to the

an increased centrifugal force applied to solve the word "rubber-elastic" is expressed,

the connection and to detach the piston from it by pressing together a seal between

Stopper as well as the downward movement of the stopper see the container wall and the piston arises

through the easy phase. 15 and the piston through during the downward movement

Many piston constructions can be used in the application of the acting force and the resulting liquid order according to the invention. It is deformable keitsdruck. Plastic, rubber and all that is required is that the piston is rubber - various combinations of both materials are elastic and have at least one part which has been found to be suitable for these purposes. To the diameter is larger than the inner sample - ao example is styrene-butadiene with a tensile strength container diameter. This makes it possible for those of about 18 to 70 kg / cm ^{2 to be} very suitable. For the piston to be squeezed into place in the tube, the piston is displaced by centrifugal force and sj forms a seal. The piston can have the and for stopping the movement in the desired shape of a massive elastic cylinder, th position in a two-phase blood fluid should be as in FIG. 2 to 4 shown. The piston can also be designed in the shape of a tricorn, preferably with a specific weight. The external shape is intermediate that of the two blood phases, generally cylindrical, however parts of the material have been removed from the cylinder to produce 1.045 to 1.09, but these values are not indispensable for an upper one Sealing surface70 and one borrowed. For example, there can be a higher centrifugal force with lower part72. In the piston shown in Fig. 6, a lighter piston or a heavier piston, the upper part serves as a sealing part, and the wcr piston used with a lower centrifugal force is tapered downward from then on. the. The piston material should not be used for the serum

The stopper or plasma produced in a molding process have permeable pores and at 146 with the piston 148 in FIG. 5 has to be at the top best impermeable to the blood cells. In the portion of the piston integrally connected to it, the specific gravity of the piston is generally disc 152 which engages the interior of the tube container between the specific gravity of the serum or to achieve the seal. Since plasmas and the cells are chosen, whereby an auto or remaining piston 148 has a smaller diameter mechanical stop of the piston on the correspond than the disc, which results after loosening the phase separation line, but that is how link 150 takes place downward movement 40 above mentioned, not essential.
of the piston, the liquid sweeps past. If the material is less elastic, a recess in the piston can be deformed around the largest part of the piston without seeing it; the deformation takes place exclusively in order to facilitate its deformability,
borrowed across disk 152. Many also suitable piston shapes, while intended for purposes of illustration, could be designed by those skilled in the art 45 securing the piston to overlying stops. Attachment of the piston to the overlying stop

An additional feature to improve fen has been pointed out, but it is clear that aucli

the mode of operation is shown in FIG. 5 and 5 A shown. other constructions are successfully applicable

Fig. 5A is a bottom view of the assembly. B. the disk 152 shown in FIG. 8

voltage according to Fig. 5. As seen from Fig. 2, 50 of the piston _a m ground 148 instead of to the upper Since "

between the bottom of the piston 46 and the top or at a point between the top and the bottom

surface of the light phase 44, a space 80 can be provided. Also where the stopper piston

present. In general, this intermediate combination is used and these parts gan;

raum80 some air and gases, which are connected with each other from the blood (Fig. 8), can do more

develop sample. The plug-piston combination 55 rere connections 150 are provided,

Figures 5 and 5A show a preferred mode of alternating throughout the description

Ventilate these gases and switch them off using the term "withdrawal" or "terminate"

possible delay influence, which this on the supply «of the acting force. With regard to di

downward moving piston through a jam representation in F i g. 2 Ws 4 means this expression!

Can have an effect. The piston 148 has a 60 stopping an exerted on the rod 30

Main passage 82 and supplementary passages 84 force downward. When displaying with Zen

Provided that are provided with a vent 86 in centrifugal force, additional factors must be taken into account

Connected. When the piston 148 can be viewed downward. The movement of a given

the gases moving in the passage 82 piston is generally a function of the speci

up and apply enough pressure to fish through 65 the weight of the piston and the dimensions

the vent 86 to below the disk 152 to remove the centrifugal force. For a given piston

give way. The disk 152 is provided with a pressure expression with a specific weight between the d «

The same channel 88 is provided, which moves according to a serum and that of the cells a pre-given

Centrifugal force pushes the piston to the phase boundary and no further. In fact, it does not apply when it is reached this position the effect of the centrifugal force, even if the centrifuge continues to run, and the centrifugal force persists. In this sense, the words "withdrawal" or "termination" are those used Power to understand. There is therefore no need to switch off the centrifuge immediately.

For the sake of clarity of the presentation, the course of the procedure was described in such a way that only the blood

phase separation and then the downward movement of the piston takes place. The downward movement of the piston in the space 80 (FIG. 2) without immersion in the blood is permitted during the phase separation and - does not affect the effectiveness of the procedure and the arrangement according to the invention.

The repeated reference to the "downward" movement of the piston through the liquid is significant of course the movement into the container without regard to the container orientation.

1 sheet of drawings

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Claims (1)

1 2 Patpntanc "-. . ^ Slidable piston which under the action of a raiemansprucne. au {^ n practiced jcraft up to almost the limit 1. Device for separating a liquid surface of the two phases in the sample container keitsprobe, in particular blood, into a light one that can be moved into it.
a heavy phase due to sedimentation or 5 The clinical evaluation of biological liquids centrifugation and to seal the separated th, such as blood, has long been an important phase against each other, consisting of a component of median investigation methods, closable by a stopper, as centrifugal Examinations provide information about the patient's state of health, which is not available on or from a different way on the inner wall of the sample container a person area of the two phases in the sample container circle that requires diagnosis and treatment of a considerable force exerted on him up to approximately the border medical supervision. The careful clinical examination is capable of being moved in, as a result of which it often distinguishes between healthy η et that the piston (46; 64; 128) is made of a rubber-elastic material from productive life and long illness, permanent invalidity or Death. and under the effect of a lengthways As a result of the importance of the analysis of the depths the of the sample container (40) acting centrifugal number of tests to be carried out annually radially deformable by virtue of the sample liquid ao aa. A recently published report by the public and can be flown around. loaned to the United States Health Service