DE1297361B - Device for blood testing by counting the blood cells - Google Patents

Description

1 2

The invention relates to a device for taking on capillary tubes of certain dimensions and diluting a liquid sample, properly stipulated, so that in this respect too special a blood sample, consisting of a self, any possibility of error is avoided.

The device is expediently specified in accordance with the automatically filling capillary tube Content and a connectable, completely or 5 of a further development of the invention designed in such a way that

partially elastic container, in which the liquid that a closure arranged in the container a

sample using negative pressure transfer head, the diameter of which is larger than the bore

is cash. of the neck portion, and has a plug which

This device is primarily used to pass through this bore into the bore of the

Blood test by counting the blood corpuscles- io muff protrudes. The sleeve can be used as a handle

chen, and it is essentially a and marking carrier serving approach have,

new pipette, which the achievable accuracy when using such a device

Dilution is much better designed. In addition, one will generally proceed in such a way that the blood

the device is inexpensive to manufacture and easy to merely pass through from the patient's skin surface

serve. Because of the cheapness of manufacture, 15 capillary action is required until completely filled into the

the pipette used only once and then thrown away. Capillary tube inserted, this attached to the container

This will avoid the risk of infection. set and into the latter by negative pressure

There is also no need to clean this device, whereupon the resulting blood mixture is conveyed

as it was previously required. The device can be opened by squeezing the elastic container

even by inexperienced persons with the blood under- ao or part of the container through the capillary tube for further

search can be used. ren processing can be submitted.

There are already mixing pipettes for such purposes. In the drawing, the subject of the invention is

known, for example from the USA patent, for example and shown schematically. It shows

2737 812. This pipette must with the relevant Fig. 1 a three-dimensional representation of the pipette,

Liquid sample, in particular blood, up to a side view of the pipette, partially

be filled with a certain mark, which is achieved by cutting,

sucking happens. Then the required dilution is Fig. 3 the rear end of a modified form

liquid in a chamber up to a form of execution.

The pipette according to FIGS. 1 and 2 consists of

can be done. The sucking up of the blood happens 30 a capillary tube 15, the inner end in a

in this known device is also used by capillary sleeve 16, the other end to a

Effect so that the amount of the liquid sample container 17 is connected. The tube 15 can be made

due to the dimensions of the capillary tube are made of solid plastic. However, it is advantageous if it is made of glass

is laid. However, the dilution liquid is produced there. The sleeve 16 is advantageously made of plastic

by brought into the mixing room that the 35 produced. The container 17 must be compressible

lower end of the capillary tube in the relevant to change its interior. He exists

Liquid immersed and at the top of the pipette made of plastic, such as trifluoro-monochlorethylene or

is sucked. In this way, the blood sample is another suitable copolymer, so that

together with the diluent in the mixing the diluent provided in the container 17

Space transported into it, and it is necessary to maintain 40 lossless over a long period of time. The wan

the absorbed amount of the dilution liquid manure of the container 17 must be thin enough so

to be determined exactly. Because this quantity is flexible and elastic due to the connection.

suction process is influenced, must be used in the operation. The front end of the sleeve 16 is in diameter

this pipette must be worked very carefully. Only reduced and advantageous as a plug-in approach 18

with sufficient experience, 45 weakly conical designs can be achieved here. From the front wall of the

achieve free results. Insertion approach 18 protrudes a plug 19 in

The known pipette thus has certain disadvantages, the bore of which the capillary tube 15 is inserted. on, which can be avoided if you have a pre-On the plug-in attachment 18 a capillary direction used according to the invention. It is tube 15 surrounding protective sleeve 20 attached anywhere below to the 50 den that can be achieved. The rear end 21 of the sleeve 16 is also Benefits received. decreased in diameter and is in the neck 22

According to the invention, the device is inserted from the container 17 in this way. The middle part of the

formed that the container is a certain fixed sleeve 16 is advantageous with a serving as a handle

Relation to the content of the capillary tube standing extension 23 'provided, on which a label 23 is attached.

Volume contains dilution liquid and that 55 can be brought with the name of the patient

for airtight connection of the capillary tube with the and other data can be labeled. In the container

Container a connecting sleeve is provided, the 17 of which is a diluent 24 is provided, the

Ends alternately in the neck part of the container texture depending on the type of desired

Sealing can be introduced, the inner bore of which is directed a count and the amount of which depends on the

has a clear width several times greater than the capillary 60 volume of the capillary tube 15 directs. In the

tube and in its one position opposite the container 17 protrudes the head piece 25 of a stopper

Interior of the container by a detachable connection 26. The plug 26 is in the bore of the sleeve 16

Finally lockable. inserted and seals it. The head piece 25

It is therefore a priori in this device that it is larger in diameter than the rest of the stopper 26

a very specific amount of the diluent 65 and also as the bore of the neck 22. Accordingly

provided so that a mistake by inattentive can in the position according to the F i g. 1 and 2 that

Operation cannot arise. The amount of diluent 24 from container 17 is not in

Blood sample is also obtained by using a bore in sleeve 16. Will the socket

Claims (1)

3 4 16 removed from the container 17, then the tube for counting red blood cells Plug 26 out of the bore of the sleeve 16 - a volume of 0.013 cm ^s and when counting drawn and falls into the interior of the container 17, of white blood cells a volume of if this is held vertically. Can accommodate 0.0013 cm ^3. If the container is to be made of glass, then its end is advantageous as in FIG. 3 shown formed. it is so easy that even a negative pressure or positive pressure does not break a relatively hard surface when dropped on order in the container 27, to be able to produce, it is at the end with a more open, regardless of whether the glass capillary tube NEN neck provided 28th A rubber ball is attached to it or it is made of plastic if its outside diameter is to be attached. By squeezing and loosing knives, do not exceed 1 mm. If the volume of the rubber ball 29 is the same, the lumina of the capillary tubes are different, depending on how they are pressed together and released, whether they are used for counting red or white containers 17 according to FIG. 1 and 2. The pre-blood cells are used, so the other end of the vitreous body 27 is again provided with a container of the same size, and the amount of the sleeve 16 made of plastic, the capillary 15 thinner in the containers is the same size,
carrying tube made of glass. The blood rises in the capillary tube until it The thin wall of the capillary tube 15 fills this completely. The penetration speed thereby achieved that a clean pipe with greater speed and the penetration pressure of the liquid in the Hole being drawn in a flame. Is the capillary tube depends on the surface tension and Diameter reduced in the desired manner, so ao the contact angle of the speed. A Verre the pipe after scratching with a diamond enlarging the edge angle should be avoided if possible or the like canceled. In this way it will be achieved. Is the inner surface of the bore of the capillary the end of the tube 15 is absolutely clean. The pipe is completely clean, so no film forms The outer diameter of the tube 15 should be smaller than this area. It has been found that films whose than that of the drop of blood, with which the clean end 95 thickness is only the thickness of a molecule, the of the tube 15 is brought into contact. The properties of the bore surface significantly The outer diameter of the tube 15 is advantageously changes. If the outer end of the pipe is thin-walled, 0.9 mm with an up and down tolerance of so there is a sharp edge that approximates the edge 0.025 mm. The inside diameter of the tube is reduced or eliminated entirely, so that the angle 15 should advantageously be about 0.7 mm with a tolerance of 30 adhesion tension or the wetting force should be large up and down by 0.01 mm. is. The surface of the edge created by breaking off In this way it is achieved that the capacity of the tube is achieved, has crystalline structure, so that of the tube 15 is directly proportional to its length. protect them from the outer and inner wall of the pipe With the dimensions given above, there is a difference. The contact angle of a liquid like ideal length of the pipe if the pipe content is 35 water with such a clean and crystalline 0.013 cm ³ with an up and down surface tolerance is close to zero. This is the reason, of 0.0002 cm ³ . The diluent in why capillary tubes made of glass those made of plastic Containers 17, 27 may vary depending on which are to be under-
seeking liquid. When doing the blood test, for
which the pipette is primarily intended for is 40 the volume of the diluent in the sub-claims:
Search of erythrocytes 2.609 cm ³ with an upward and downward tolerance of 0.031 cm ³ , while!. Device for receiving and diluting in the examination of leukocytes of this one liquid sample, in particular a blood lumen 0.2609 cm ³ with an upward tolerance and 45 sample consisting of a self-acting down of 0.0031 cm ³ should be. If the erythro-filling capillary tube is to be counted precisely defined content cytes, the dilution and a connectable, complete or partial solution consists of an elastic container into which the liquid,. ". " _{T nnn} . test using negative pressure distilled water 200cm ³ , _{$ 0 is} transferable, thus marked Sodium chlorite Ig, η e t that the container (17) is in a certain Sodium sulphate 5 g, fixed ratio to the contents of the capillary tube Mercury chlorite 0.5 g. 0- $) standing volume of diluent contains and that for airtight connection of the When counting leucocytes usually 55 capillary tube (15) with the container (17) becomes one Water with 1% hydrochloric acid used. Connection sleeve (16) is provided, the Instead of capillary tubes of the same dimensions for the ends (18, 21) alternately in the neck part (22) Counting red and white blood cells to the container (17) can be inserted in a sealing manner, use and the dilution liquid in the inside of the bean wash a several times larger halter to change accordingly in the amount, has 60 width than the capillary tube (15) and the in the reverse can also be used. In their one position in relation to the interior The counting of red blood cells is supposed to be done by the container through a detachable lock 1 part blood to 200 parts dilution (25) can be shut off. fluid and when counting white blood 2. Device according to claim 1, characterized k involved 1 part blood to 20 parts dilution 65 indicates that there is a liquid in the container (17) be. Then, with regard to the neter closure (25), a head, its diameter and / or the length differently knife larger than the bore of the neck part (22) If large capillary tubes are used, it should be capillary and have a plug (26) that passes through this bore protrudes through into the bore of the sleeve (16). 3. Apparatus according to claim 1 or 2, characterized in that the sleeve (16) has a as a handle and marker carrier serving approach (23 ^. 1 sheet of drawings