DE120580C - - Google Patents
Info
- Publication number
- DE120580C DE120580C DENDAT120580D DE120580DA DE120580C DE 120580 C DE120580 C DE 120580C DE NDAT120580 D DENDAT120580 D DE NDAT120580D DE 120580D A DE120580D A DE 120580DA DE 120580 C DE120580 C DE 120580C
- Authority
- DE
- Germany
- Prior art keywords
- iron
- yeast
- content
- preparation
- until
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 46
- 229910052742 iron Inorganic materials 0.000 claims description 23
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 210000004940 Nucleus Anatomy 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N HCl Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- 210000004051 Gastric Juice Anatomy 0.000 description 5
- NPFOYSMITVOQOS-UHFFFAOYSA-K 2-hydroxypropane-1,2,3-tricarboxylate;iron(3+) Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000004027 cells Anatomy 0.000 description 3
- 238000005755 formation reaction Methods 0.000 description 3
- 210000002784 Stomach Anatomy 0.000 description 2
- 230000001488 breeding Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 150000002506 iron compounds Chemical class 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 210000004369 Blood Anatomy 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N Carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 210000003855 Cell Nucleus Anatomy 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 210000002969 Egg Yolk Anatomy 0.000 description 1
- 210000001156 Gastric Mucosa Anatomy 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 229940082629 IRON ANTIANEMIC PREPARATIONS Drugs 0.000 description 1
- 210000001747 Pupil Anatomy 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000612118 Samolus valerandi Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000001419 dependent Effects 0.000 description 1
- 230000001079 digestive Effects 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/28—Compounds containing heavy metals
- A61K31/295—Iron group metal compounds
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
KAISERLICHESIMPERIAL
PATENTAMT.PATENT OFFICE.
Es ist in der letzten Zeit, entgegen den früheren Anschauungen Bunge's und seiner Schüler, bewiesen worden, dafs auch anorganisch gebundenes Eisen vom thierischen Organismus resörbirt wird, dafs dagegen organisch gebundenes Eisen die Blutbildung stärker anregt, leichter zur Hämoglobinbildung führt als anorganisch gebundenes Eisen. Ferner hat sich . ergeben, dafs die Eisenpräparate unzersetzt und ohne Lösung der Eisenverbindung durch den Magen hindurchgehen müssen, damit keine Schädigung des Magens, welche so oft die Eisenmedication begleitet, eintritt. Dieser Forderung genügt keine der bisherigen Eisenverbindungen. .It is of late, contrary to the earlier views of Bunge and himself Pupil, it has been shown that inorganically bound iron is also from the animal organism it is absorbed that, on the other hand, organically bound iron stimulates the formation of blood more strongly, leads to hemoglobin formation more easily than inorganically bound iron. Furthermore has . show that the iron preparations are undecomposed and without dissolution of the iron compound by the Must go through the stomach so as not to damage the stomach, which so often the Accompanied iron medication, occurs. None of the previous iron compounds met this requirement. .
Wir suchten nun ein Präparat darzustellen, welches schon mit Magensaft verdaut worden ist und somit kein in der Salzsäure des Magensaftes abspaltbares und lösliches Eisen mehr enthält, und welches ferner das Eisen in der Form, in welcher es in der Nahrung vorkommt, nämlich als Nuclei'nverbindung enthält, cWe tried to present a preparation which had already been digested with gastric juice and therefore no longer any iron that can be split off and soluble in the hydrochloric acid of the gastric juice and which also contains iron in the form in which it occurs in food, namely contains as a nucleus compound, c
Wir waren also bestrebt, eisenhaltige Nucle'ine darzustellen, die das Eisen etwa in derselben Weise gebunden enthalten, wie die Nucle'ine des Eidotters. Durch Versuche hat Fromme festgestellt, dafs Bacterien auf eisenhaltiger Gelatine gezüchtet werden können. (Fromme hatte durch dieses Züchtungsverfahren die ■ Bildung des Schwefelwasserstoffes nachweisen ■wollen.) Diese Beobachtung benutzen wir, "um unter Zuhülfenahme der Lebensthätigkeit der Zelle solche eisenhaltige Nucleoalbumine zu erhalten.So we endeavored to represent iron-containing nucle'ins, which iron about the same Contained in bound manner, like the nucle'ins of the egg yolk. Through experimentation pious has found that bacteria can be grown on gelatin containing iron. (Pious had to prove the formation of hydrogen sulphide through this breeding process ■ want.) We use this observation "to" with the aid of vital activity the cell to obtain such iron-containing nucleoalbumins.
Wir züchten also Hefe auf eisenhaltigem Nährboden, filtriren nach einiger Zeit ab und unterwerfen den Filterrückstand, der aus Hefezellen· besteht, der Verdauung mit künstlichem Magensafte. Nach vollendeter Verdauung werden die zurückgebliebenen Hefekerne abfiltrirt, mit 0,3 proc. Salzsäure so lange ausgewaschen, bis kein Eisen mehr in Lösung geht, und dann getrocknet.So we grow yeast on a ferrous medium, and after a while we filter it off and subject the filter residue, which consists of yeast cells, to digestion with artificial Gastric juices. After digestion is complete, the remaining yeast kernels are filtered off, with 0.3 proc. Hydrochloric acid washed out until no more iron goes into solution, and then dried.
Das so dargestellte Präparat ist eine eisenhaltige Nuclemverbindung, in welcher das Eisen mehr oder weniger organisch gebunden ist. Der Eisengehalt kann bis zwei und mehr Procent betragen, während gleich behandelte Hefe ohne Eisenzusatz, wenn man davon 5 g einäschert, nur unbestimmbare Eisenmengen enthält. The preparation thus represented is an iron-containing nucleus compound in which the iron is more or less organically bound. The iron content can be up to two percent or more, while yeast is treated in the same way without added iron, if 5 g of it is cremated, it contains only indeterminable amounts of iron.
Es ist sehr schwierig, im Voraus den Eisengehalt eines so dargestellten Präparates anzugeben, da dieser Gehalt hauptsächlich von der Lebensintensität der Hefe abhängig ist. Ferner sind von Einflufs der Eisengehalt der Nährflüssigkeit sowie die Dauer der Einwirkung in dem Sinne, dafs, wenn die Nährflüssigkeit 2 bis 3 pCt. Eisen enthält, ein Optimum eintritt; ebenso bedeutet eine Verlängerung der Züchtungszeit, wenn dabei keine Fäulnifs eintritt, ein Steigen des Eisengehaltes des Endproductes. It is very difficult to indicate in advance the iron content of a preparation presented in this way, since this content is mainly dependent on the life intensity of the yeast. Further are influenced by the iron content of the nutrient fluid and the duration of the action in the sense that if the nutrient fluid is 2 to 3 pCt. Contains iron, an optimum occurs; also means an extension of the breeding time, if there is no putrefaction, an increase in the iron content of the end product.
Die angewendeten Mengenverhältnisse und Arbeitsbedingungen sind aus folgenden Beispielen ersichtlich. Selbstverständlich kann man sowohl die Mengenverhältnisse als auch die Arbeitsweise stark yariiren.The proportions and working conditions used are from the following examples evident. Of course, you can use both the proportions and the Strongly yariate the way of working.
■Beispiel I.■ Example I.
ίο 1 frischer Bierhefe mit etwa 20 pCt. Trockengehalt werden mit 100 bis 500 g Eisenciträt und 500 bis 5000 g Traubenzucker versetzt und etwa 8 Tage stehen gelassen. Dann wird die Flüssigkeit abcolirt und der feste Rückstand mit 10 1 künstlichem. Magensafte (dargestellt aus Schweinsmagenschleimhaut mit Salzsäure) so lange umgerührt, bis die Eiweifsstoffe der Zellen verdaut und unter dem Mikroskrop nur noch die isolirten Zellenkerne sichtbar sind. Dies dauert etwa 10 Stunden. Der Verdauungsrückstand wird abfütrirt und so lange mit 0,3 proc. Salzsäure gewaschen, bis kein Eisen mehr in Lösung geht; das Product wird dann getrocknet und gepulvert.ίο 1 fresh brewer's yeast with about 20 pCt. Dry content is 100 to 500 g of iron citrate and 500 to 5000 g of glucose are added and left to stand for about 8 days. Then the liquid is isolated and the solid 10 1 artificial residue. Gastric juices (shown from pig gastric mucosa with Hydrochloric acid) until the proteins in the cells are digested and under the Microscope only the isolated cell nuclei are visible. This takes about 10 hours. The digestive residue is drained off and so long with 0.3 per cent. Hydrochloric acid washed, until no more iron goes into solution; the product is then dried and powdered.
Eine Nährbouillon wird mit 1J2 pCt. Eisencitrat und so viel Soda versetzt, bis der durch das Eisencitrat bewirkte Niederschlag wieder in Lösung geht, das Ganze wird dann sterilisirt. Darauf wird die Lösung mit einer Reincultur einer Hefenart, z. B. Saccharomyces apiculatus, geimpft und stehen gelassen, bis sich eine genügende Menge der Gultur entwickelt hat. Diese wird dann abfiltrirt, mit künstlichem Magensafte verdaut und; überhaupt wie in Beispiel I weiter behandelt.'A nutrient broth is made with 1 J 2 pCt. Iron citrate and so much soda are added until the precipitate caused by the iron citrate goes into solution again, the whole thing is then sterilized. The solution is then mixed with a pure culture of a yeast species, e.g. B. Saccharomyces apiculatus, inoculated and allowed to stand until a sufficient amount of the culture has developed. This is then filtered off, digested with artificial gastric juice and; treated as in Example I. '
Claims (1)
Publications (1)
Publication Number | Publication Date |
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DE120580C true DE120580C (en) |
Family
ID=389630
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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DENDAT120580D Active DE120580C (en) |
Country Status (1)
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DE (1) | DE120580C (en) |
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