DE1130678B - Growth-promoting feed - Google Patents

Description

GERMAN

PATENT OFFICE

C 18358 IVa / 53 g

REGISTRATION DATE: 7TH F E B RU AR 1959

NOTICE
THE REGISTRATION
ANDOUTPUTE
EDITORIAL: MAY 30, 1962

In recent years, extensive research has been carried out on the usefulness of various food components as an additive to poultry and cattle feed, leading to the development of feed containing all essential nutrients, such as: B. proteins, carbohydrates, mineral salts and vitamins in balanced proportions. Feeds for special purposes have also been developed. Further investigations in the field of animal feed led to the discovery that by adding substances such. B. vitamin B _1. , For feed the growth rate of the cattle is greatly increased and that these substances act as growth accelerators.

It has recently also been found that the addition of certain antibiotics to animal feed causes a growth effect which is even stronger than when vitamin B _{12 is added} alone. It is known to use the dried fermentation liquid of Streptomyces aurofaciens as supplementary feed. This discovery made it possible to breed animals that are distinguished by their particular size and strength from a young age. In this context, the feeding of the evaporated fermentation liquid from which aureomycin has been removed has already been described. The recovery of such a dried-up residue is, however, relatively laborious, because the aureomycin must first be separated off in a complicated manner and only then the fermentation liquid must be evaporated, but on the other hand the use of a supplementary feed in dry form is preferable because it is easier to mix with the main feed.

On the other hand, it is already known to feed mold mycelia.

In contrast, the invention is based on the knowledge that the feeding of fermentation residues, those in the known production of gibberellic acid by cultivating the small mushroom »Gibberella fujikuroi «fall in a liquid nutrient medium and which consist of the substances that are after the Separation of the gibberellic acid remaining in the nutrient medium causes such a strong growth effect that normally occurs when feeding a balanced feed with the addition of these residues could not be expected.

The subject of the invention is therefore a growth-promoting Complementary feed that contains the fermentation residue as an active ingredient Cultivation of a gibberellic acid producing small mushroom in a liquid nutrient medium after growth-promoting Complementary feed

Applicant:

Commercial Solvents Corporation, Terre Haute, Ind. (V. St. A.)

Representative: Dr. H.-H. Willrath, patent attorney, Wiesbaden, Hildastr. 32

Joseph M. Pensack, Terre Haute, Ind., And Francis W. Chornock, Halstead, Kan.

(V. St. A.),
have been named as inventors

separation of the gibberellic acid is obtained. The production is preferably carried out by one Gibberellic acid producing strain of the small mushroom »Gibberella fujikuroi« in a liquid nutrient medium cultivates in a manner known per se, the gibberellic acid formed is removed by filtration and the fermentation residue dries.

The usability of fermentation residues from gibberellic acid production as supplementary feed was all the less to be expected, since feeding experiments with gibberellic acid are not even possible in poultry led to a growth-promoting effect, but on the other hand the fermentation residue is, as it were, a Waste product from the recovery of gibberellic acid, which is valuable in other respects; this fermentation residue differs fundamentally from the fermentation liquids of Streptomyces aureofaciens in aureomycin extraction, because this microorganism belongs to the class of streptomycetes, while "Gibberella fujikuroi" is a mold that resembles the penicillin mushrooms and provides a relatively dry fungal network, which can be separated by simple filtration.

In addition, the supplementary feed according to the invention also has a stronger growth

209 607/135

promotion supplies. A supplementary feed of 3% dried fermentation liquid from Streptomyces aureofaeiens resulted in z. B. Chick in a weight gain with respect to a chuck with 10.5 mg Vitamin B ₁₂ of 58 g and with respect to a chuck with 17.5 mg Vit B ₁₂ g of amine 46th In contrast, test chicks after feeding with the addition of the fermentation residue from gibberellic acid production in an amount of 2% showed a weight gain of 49.5 and 60.1 g, respectively, compared to the feeding of feed containing 13 mg of vitamin B ₁₂ .

The gibberellic acid fermentation residues used according to the invention can by fermentation with the help of the small mushroom of the species »Gibberella fujikuroi« or its asexual form »Fusarium moniliforme« under conditions which are comparable in the production of riboflavin adhered to by fermentation can be obtained. For example, the nutrient solution in the fermentation vat, which contains adequate amounts of carbohydrates, such as cane sugar, Starch, molasses, etc., also yeast extract or meat broth, a lipoid such as bacon oil and various inorganic ones Contains salts, inoculated with the small mushroom »Gibberella fujikuroi«.

The fermentation medium is previously adjusted with ammonia to p _H 6 and then moved by introducing air in a ratio of 0.5 volumes of air to 1 part by volume of mash and held for 5 to 6 days at 28 ° C. The gibberellic acid is then separated off by filtration. The remaining fungal network is dried. It contains 10 to 22% protein and 4 to 35% fat.

Feeding experiments showed that the feed contained at least 0.25% gibberellic acid fermentation residues must be added to achieve an effective growth rate in poultry. Over 2% fermentation residues are necessary to achieve the maximum growth rate in chicks.

The following table gives the minimum amounts of fermentation residue for an effective growth rate in different animals:

A normal chick feed with the following composition was used to test growth promotion prepares:

Yellow corn flour 27.519 kg

Oily soy flour (44% soluble

Components) 15.855 kg

Dicalcium phosphate 906 g

Ground limestone 679 g

Iodized salt 227 g

DL-methionine 45.3 g

Choline chloride, 70% 28.3 g

Butylated hydroxytoluene 5.6 g

Manganese sulfate, 70% 11.3 g

Vitamin A addition (10,000 IU / gm) 40 g vitamin D ₃ addition (1500 IGU *) /

gm) 27.5 g

Niacin 1.5 g

Calcium pantothenate 0.5 g

Crystalline zinc bacitracin 0.375 g

Crystalline procaine penicillin 0.125 g

Crystalline riboflavin 0.3 g

Crystalline vitamin B ₁₂ 0.60 g

*) International Chick Unit.

Calculated composition Nutritional value .... 1970 kcal / kg 21.1% protein Riboflavin ... 8.26 mg / kg 2.48% fat ChIoHn 1.572 g / kg 3.79% crude fiber niacin 55.3 mg / kg 1.18% calcium Pantothenic acid 18.7 mg / kg 1.74% phosphorus Vitamin A ... 10178 IU / kg 1.22% arginine Vitamin D ₃ .. 911 ICU / kg 0.50% methionine Calorie 0.31% cystine Protein- Relationship .. 42.4 1.06% lysine 0.23% tryptophan 1.00% glycine

More necessary ¹ TTi »Ti» Fermentation residue in ° / o I IcFc 0.5 Calves 0.5 lambs 0.5 Pigs 0.5 Turkey 0.5 Ducks 0.25 chick

Part of this feed was made with 1% soluble fish meal and 1% dried whey, another Part of the feed mixed with 2% fermentation residue. Mixing in fish meal and dried Whey had the purpose of making a comparison between that in fish meal and in whey as well bring about the growth factors present in the fermentation residues. There were two series of tests performed with two groups of twelve male Vantress-Thompson-White-Rock chicks each. After 28 days the chicks were weighed.

Chick experiment I mean weight gain in grams (28 days experiment)

Feed mix Group 1 Group 2 average Weight gain
in ° / o Normal feed 421.7
435.1
471.8 399.4
443.8
448.2 410.5
439.4
460.0 -j-1% fish meal and
1% dried whey
+ 2% fermentation residues 7.0
12.0

Chick experiment II

Feed mix Group 1 Group 2 average Weight gain
in ° / o Normal feed
+1% fish meal and
1% dried whey
+ 2 ° / o fermentation residues 395.2
394.2
461.2 370.9
444.0
425.0 383.0
419.1
443.1 9.4
15.6

The following table shows the ratios of the amount of feed fed to the weight gain achieved specified.

Chick Try I
Amount of feed in grams, weight gain in grams

Feed mix Group 1 Group 2 average Improvement of
Feed conversion
opposite to
normal feed Normal feed
+1% fish meal and
1% dried whey
+ 2% fermentation residues 2.09
2.17
2.02 2.50
2.28
2.25 2.30
2.22
2.14 3.4
6.9

Chick experiment II

Feed mix Group 1 Group 2 average Feed conversion
in ° / o Normal feed
+1% fish meal and
1% dried whey
+ 2% fermentation residues 2.27
2.28
1.94 2.28
1.94
1.94 2.28
2.11
1.96 7.4
14.0

Claims (2)

PATENT CLAIMS: 1. Growth- promoting supplementary feed, characterized in that it contains the fermentation residue as an active component, which is obtained in the cultivation of a gibberellic acid-producing small mushroom in a liquid nutrient medium after the gibberellic acid has been separated off. 2. A method for the production of a supplementary feed according to claim 1, characterized in that that a strain of the small mushroom "Gibberella fujikuroi" producing gibberic acid is found in a liquid nutrient medium cultivates the gibbereuic acid formed in a manner known per se removed by filtration and the fermentation residue dries. Considered publications:
German Patent Nos. 880 540, 695 107;
British Patent No. 803 591. © 209 607/135 5.