DE112018003256A5 - Methode zur gezielten Identifizierung hocheffizienter "small interfering RNAs" ("eRNAS" zur Anwendung in Pflanzen und anderen Zielorganismen - Google Patents

Methode zur gezielten Identifizierung hocheffizienter "small interfering RNAs" ("eRNAS" zur Anwendung in Pflanzen und anderen Zielorganismen Download PDF

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Publication number
DE112018003256A5
DE112018003256A5 DE112018003256.6T DE112018003256T DE112018003256A5 DE 112018003256 A5 DE112018003256 A5 DE 112018003256A5 DE 112018003256 T DE112018003256 T DE 112018003256T DE 112018003256 A5 DE112018003256 A5 DE 112018003256A5
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Prior art keywords
ernas
plants
highly efficient
small interfering
interfering rnas
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Withdrawn
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DE112018003256.6T
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English (en)
Inventor
Sven-Erik Behrens
Torsten Gursinsky
Jana Schuck
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Martin Luther Univ Halle Wittenberg
Martin Luther Universitaet Halle Wittenberg
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Martin Luther Univ Halle Wittenberg
Martin Luther Universitaet Halle Wittenberg
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Publication of DE112018003256A5 publication Critical patent/DE112018003256A5/de
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE112018003256.6T 2017-06-26 2018-06-22 Methode zur gezielten Identifizierung hocheffizienter "small interfering RNAs" ("eRNAS" zur Anwendung in Pflanzen und anderen Zielorganismen Withdrawn DE112018003256A5 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE102017005957 2017-06-26
DE102017005957.8 2017-06-26
DE102018001134.9 2018-02-13
DE102018001134.9A DE102018001134A1 (de) 2017-06-26 2018-02-13 Methode zur gezielten Identifizierung hocheffizienter "small interfering RNAs" ("eRNAs") zur Anwendung in Pflanzen und anderen Zielorganismen
PCT/DE2018/000194 WO2019001602A1 (de) 2017-06-26 2018-06-22 Methode zur gezielten identifizierung hocheffizienter "small interfering rnas "ernas" zur anwendung in pflanzen und anderen zielorganismen

Publications (1)

Publication Number Publication Date
DE112018003256A5 true DE112018003256A5 (de) 2020-03-12

Family

ID=64567709

Family Applications (2)

Application Number Title Priority Date Filing Date
DE102018001134.9A Withdrawn DE102018001134A1 (de) 2017-06-26 2018-02-13 Methode zur gezielten Identifizierung hocheffizienter "small interfering RNAs" ("eRNAs") zur Anwendung in Pflanzen und anderen Zielorganismen
DE112018003256.6T Withdrawn DE112018003256A5 (de) 2017-06-26 2018-06-22 Methode zur gezielten Identifizierung hocheffizienter "small interfering RNAs" ("eRNAS" zur Anwendung in Pflanzen und anderen Zielorganismen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE102018001134.9A Withdrawn DE102018001134A1 (de) 2017-06-26 2018-02-13 Methode zur gezielten Identifizierung hocheffizienter "small interfering RNAs" ("eRNAs") zur Anwendung in Pflanzen und anderen Zielorganismen

Country Status (3)

Country Link
EP (1) EP3645729A1 (de)
DE (2) DE102018001134A1 (de)
WO (1) WO2019001602A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102021107508B4 (de) 2021-03-25 2023-06-15 Martin-Luther-Universität Halle-Wittenberg, Körperschaft des öffentlichen Rechts Zuverlässige Identifikation von Bereichen (,a-Sites') in komlexen RNA-Molekülen, die zugänglich sind für Nukleinsäuren oder Komplexe von Nukleinsäuren mit Endonukleasen
EP4365297A1 (de) 2022-11-07 2024-05-08 Martin-Luther-Universität Halle-Wittenberg Nukleinsäurewirkstoffe gegen verschiedene pflanzenpathogene

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2360253B1 (de) * 2000-03-30 2014-03-19 The Whitehead Institute for Biomedical Research Verfahren zur Herstellung von Knockdown-Zellen oder -Organismen mittels RNS-sequenzspezifischer Mediatoren von RNS-Interferenz und deren Verwendung

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Publication number Publication date
WO2019001602A1 (de) 2019-01-03
DE102018001134A1 (de) 2018-12-27
EP3645729A1 (de) 2020-05-06

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