DE1101415B - Process for the preparation of 6ª ‡ -Methyl-17ª ‡ -acetoxy-4-pregnen-3, 20-dione - Google Patents

Process for the preparation of 6ª ‡ -Methyl-17ª ‡ -acetoxy-4-pregnen-3, 20-dione

Info

Publication number
DE1101415B
DE1101415B DES59963A DES0059963A DE1101415B DE 1101415 B DE1101415 B DE 1101415B DE S59963 A DES59963 A DE S59963A DE S0059963 A DES0059963 A DE S0059963A DE 1101415 B DE1101415 B DE 1101415B
Authority
DE
Germany
Prior art keywords
methyl
pregnen
dione
acetoxy
oxy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DES59963A
Other languages
German (de)
Inventor
Luis E Miramontes
O Fritsche
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GD Searle LLC
Original Assignee
GD Searle LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GD Searle LLC filed Critical GD Searle LLC
Publication of DE1101415B publication Critical patent/DE1101415B/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J7/00Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of two carbon atoms

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Steroid Compounds (AREA)

Description

Verfahren zur Herstellung von 6 a-Methyl-17 a-acetoxy-4-pregnen-3,20-dion Gegenstand der Erfindung ist ein Verfahren zur Herstellung von 6a-Methyl-17a-acetoxy-4-pregnen-3,20-dion der folgenden Strukturformel: Die erfindungsgemäß angewandte Ausgangsverbindung wird aus 6-Methyl-3ß-oxy-5,16-pregnadien-20-on oder dessen Estern hergestellt. Hierzu wird eine alkalische Lösung des Steroids mit einem milden Epoxydiationsmittel, wie mit alkalischem Wasserstoffperoxyd, unter Bildung von 3ß-Oxy-6-methyl-16a, 17a-epoxy-5-pregnen-20-on behandelt, das nach der Oxydation nach O p p e nauer 6a-Methyl-16a, 17a-epoxy-4-pregnen-3,20-dion ergibt.Process for the preparation of 6a-methyl-17a-acetoxy-4-pregnen-3,20-dione The invention relates to a process for the preparation of 6a-methyl-17a-acetoxy-4-pregnen-3,20-dione following structural formula: The starting compound used according to the invention is prepared from 6-methyl-3ß-oxy-5,16-pregnadien-20-one or its esters. For this purpose, an alkaline solution of the steroid is treated with a mild epoxidation agent, such as alkaline hydrogen peroxide, to form 3ß-oxy-6-methyl-16a, 17a-epoxy-5-pregnen-20-one, which after oxidation according to O ppe nauer 6a-methyl-16a, 17a-epoxy-4-pregnen-3,20-dione gives.

Diese Verbindung wird dann erfindungsgemäß durch Behandlung mit Bromwasserstoffsäure in einem organischen Lösungsmittel, z. B. Chloroform oder einer niederen Alkansäure, in 6a-Methyl-16ß-brom-17a-oxy-4-pregnen-3,20-dion umgewandelt. Das Bromhydrin wird dann durch Behandlung mit einem Hydrierungskatalysator, wie Palladium oder Raney-Nickel, in 6a-Methyl-17a-oxy-4-pregnen-3,20-dion umgewandelt. Es kann dabei auf Calciumcarbonat oder Ammoniumacetat absorbiertes Palladium verwendet werden. Zusammen mit dem Palladiumkatalysator wird eine Lösung des Bromhydrins in einem Lösungsmittel, wie Äthanol, bei Raumtemperatur gerührt, bis ein Moläquivalent Wasserstoff absorbiert worden ist. Ein anderer nützlicher Hydrierungskatalysator ist Raney-Nickel. Dieser kann in alkoholischer Lösung bei Rückflußtemperatur verwendet werden. Das erhaltene 6a-Methyl-17a-oxy-4-pregnen-3,20-dion wird anschließend durch übliche Acetylierungsmittel in das entsprechende 6a-Methyl-17a-acetoxy-5-pregnen-3,20-dion umgewandelt.This compound is then made according to the invention by treatment with hydrobromic acid in an organic solvent, e.g. B. chloroform or a lower alkanoic acid, converted to 6a-methyl-16ß-bromo-17a-oxy-4-pregnen-3,20-dione. The bromohydrin will then by treatment with a hydrogenation catalyst such as palladium or Raney nickel, converted to 6a-methyl-17a-oxy-4-pregnen-3,20-dione. It can be due to calcium carbonate or ammonium acetate absorbed palladium can be used. Together with the palladium catalyst becomes a solution of the bromohydrin in a solvent such as ethanol at room temperature stirred until one molar equivalent of hydrogen has been absorbed. Another more useful The hydrogenation catalyst is Raney nickel. This can be in alcoholic solution Reflux temperature can be used. The 6a-methyl-17a-oxy-4-pregnen-3,20-dione obtained is then converted into the corresponding 6a-methyl-17a-acetoxy-5-pregnen-3,20-dione by conventional acetylating agents converted.

Die erfindungsgemäß herstellbare Verbindung zeichnet sich durch eine überraschend hohe progestationale Wirksamkeit aus. Im Vergleich zu Progesteron zeigt das verfahrensgemäß hergestellte 17-Acetat eine 50- bis 60fache Wirksamkeit, während das entsprechende nichtmethylierte Produkt nur etwa die 6fache Wirkung des Progesterons aufweist. In dem folgenden Beispiel sind die Mengen in Gewichtsteilen angegeben.The connection that can be produced according to the invention is characterized by a surprisingly high progestational effectiveness. Compared to progesterone shows the 17-acetate prepared according to the method has a 50- to 60-fold effectiveness, while the corresponding non-methylated product only has about 6 times the effect of progesterone having. In the following example the amounts are given in parts by weight.

Beispiel Das als Ausgangsprodukt verwendete 6a-Methyl-16a, 17a-epoxy-4-pregnen-3,20-dion wurde durch Umsetzung von 3ß-Acetoxy-6-methyl-5,16-pregnadien-20-on mit alkalischer Wasserstoffperoxydlösung zu 3ß-Oxy-6-methyl-16a,17a-epoxy-5-pregnen-20-on und durch anschließende Oppenauer-Oxydation mit Cyclohexanon in Gegenwart von Aluminiumisopropylat hergestellt. Die Verbindung schmilzt bei etwa 130 bis 134° C, hat eine spezifische Drehung von +129,5° und zeigt im ultravioletten Bereich eine Absorption bei 240 m#t mit einem Extinktionskoeffizienten von etwa 15830.Example The 6a-methyl-16a, 17a-epoxy-4-pregnen-3,20-dione used as the starting material was made by reacting 3ß-acetoxy-6-methyl-5,16-pregnadien-20-one with alkaline Hydrogen peroxide solution to 3ß-Oxy-6-methyl-16a, 17a-epoxy-5-pregnen-20-one and through subsequent Oppenauer oxidation with cyclohexanone in the presence of aluminum isopropylate manufactured. The compound melts at about 130 to 134 ° C, has a specific Rotation of + 129.5 ° and shows an absorption at 240 in the ultraviolet range m # t with an extinction coefficient of about 15830.

Eine Lösung von 0,5 Teilen 6a-Methyl-16a,17a-epoxy-4-pregnen-3,20-dion in Essigsäure wird 30 Minuten mit Bromwasserstoffsäure behandelt. Das Gemisch wird in Eiswasser gegossen und das Bromhydrin durch Filtrieren gewonnen. Nach Trocknung wird das Bromhydrin in Methanol gelöst und 2 Stunden mit dem Zweifachen seines Gewichts an Raney-Nickel unter Rückfluß gehalten. Der Katalysator wird abfiltriert und die Lösung fast bis zur Trockne eingedampft. Man läßt den Rückstand 15 Stunden bei Raumtemperatur stehen. Nach Umkristallisieren der farblosen Nadeln aus einem Gemisch von Wasser und Aceton erhält man 6a-Methyl-17a-oxy-4-pregnen-3,20-dion, das bei etwa 215 bis 217° C schmilzt und eine spezifische Drehung in Chloroform von +78'C hat. Die Verbindung zeigt im ultravioletten Bereich eine Absorption bei 240 mi, mit einem Extinktionskoeffizienten von etwa 15800.A solution of 0.5 part of 6a-methyl-16a, 17a-epoxy-4-pregnen-3,20-dione in acetic acid is treated with hydrobromic acid for 30 minutes. The mixture will Poured into ice water and recovered the bromohydrin by filtration. After drying the bromohydrin is dissolved in methanol and 2 hours with twice its weight refluxed on Raney nickel. The catalyst is filtered off and the Solution evaporated almost to dryness. The residue is left at room temperature for 15 hours stand. After recrystallization of the colorless needles from a mixture 6a-methyl-17a-oxy-4-pregnen-3,20-dione is obtained from water and acetone about 215 to 217 ° C melts and a specific rotation in chloroform of + 78'C Has. The compound shows an absorption at 240 mi in the ultraviolet range, with an extinction coefficient of about 15800.

Eine Lösung von 10 Teilen 6a-Methyl-17a-oxy-4-pregnen3,20-dion in 162 Teilen Essigsäureanhydrid und 1,5 Teilen p-Toluolsulfonsäure wird bei Raumtemperatur 15 Stunden gerührt. Die Lösung wird dann in einem Eisbad gekühlt und mit 200 Teilen Wasser langsam versetzt, bis der AnhydridüberschuB zerstört ist. Die Fällung -wird abfiltriert und getrocknet. Nach Umkristallisieren aus Methanol erhält man 6a-Methyl-17a-acetoxy-4-pregnen-3,20-dion, das bei etwa 214,5 bis 216°C schmilzt. Die Verbindung zeigt im ultravioletten Bereich eine Absorption bei 214 m#t mit einem Extinktionskoeffizienten von etwa 16750.A solution of 10 parts of 6a-methyl-17a-oxy-4-pregnen3,20-dione in 162 parts of acetic anhydride and 1.5 parts of p-toluenesulfonic acid are used at room temperature Stirred for 15 hours. The solution is then cooled in an ice bath and added to 200 parts Water is slowly added until the excess anhydride is destroyed. The precipitation -will filtered off and dried. After recrystallization from methanol, 6a-methyl-17a-acetoxy-4-pregnen-3,20-dione is obtained, which melts at about 214.5 to 216 ° C. The compound shows in the ultraviolet range an absorbance at 214 m # t with an extinction coefficient of about 16750.

Claims (1)

PATENTANSPRUCH: Verfahren zur Herstellung von 6a-Methyl-17a-acetoxy-4-pregnen-3,20-dion, dadurch gekennzeichnet, daB man nach bekannten Methoden 6a-Methyl-16a,17a-epoxy-4-pregnen-3,20-dion mit Bromwasserstoffsäure behandelt, das erhaltene 6a-Methyl-16ß-brom-17a-oxy-4-pregnen-3,20-dion reduktiv entbromiert und das erhaltene Produkt in bekannter Weise acetyliert. In Betracht gezogene Druckschriften: Journ. Amer. Chem. Soc., 72,_ S. 5145 (1950).PATENT CLAIM: Process for the production of 6a-methyl-17a-acetoxy-4-pregnen-3,20-dione, characterized in that 6a-methyl-16a, 17a-epoxy-4-pregnen-3,20-dione are used according to known methods treated with hydrobromic acid, the 6a-methyl-16ß-bromo-17a-oxy-4-pregnen-3,20-dione obtained reductively entbrominated and the product obtained acetylated in a known manner. In Publications considered: Journ. Amer. Chem. Soc., 72, p. 5145 (1950).
DES59963A 1957-09-27 1958-09-24 Process for the preparation of 6ª ‡ -Methyl-17ª ‡ -acetoxy-4-pregnen-3, 20-dione Pending DE1101415B (en)

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US1101415XA 1957-09-27 1957-09-27

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DE1101415B true DE1101415B (en) 1961-03-09

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0153270A2 (en) * 1984-01-20 1985-08-28 Schering Aktiengesellschaft 1-Alpha, 2-alpha-methylene-6-methylene and 6-alpha-methyl pregnenes, process for their preparation and pharmaceutical compositions containing them

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0153270A2 (en) * 1984-01-20 1985-08-28 Schering Aktiengesellschaft 1-Alpha, 2-alpha-methylene-6-methylene and 6-alpha-methyl pregnenes, process for their preparation and pharmaceutical compositions containing them
EP0153270A3 (en) * 1984-01-20 1986-06-04 Schering Aktiengesellschaft Berlin Und Bergkamen 1-alpha, 2-alpha-methylene-6-methylene and 6-alpha-methyl pregnenes, process for their preparation and pharmaceutical compositions containing them

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