DE1068862B - Procedure for extending the shelf life of blood products - Google Patents
Procedure for extending the shelf life of blood productsInfo
- Publication number
- DE1068862B DE1068862B DENDAT1068862D DE1068862DA DE1068862B DE 1068862 B DE1068862 B DE 1068862B DE NDAT1068862 D DENDAT1068862 D DE NDAT1068862D DE 1068862D A DE1068862D A DE 1068862DA DE 1068862 B DE1068862 B DE 1068862B
- Authority
- DE
- Germany
- Prior art keywords
- blood
- shelf life
- extending
- blood products
- procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Physiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
Verfahren zur Verlängerung der Haltbarkeit von Blutkonserven Die Erfindung betrifft ein Verfahren zur Verlängerung der Haltbarkeit von Blutkonserven.Method for extending the shelf life of blood products The invention relates to a method for extending the shelf life of blood products.
Bekanntlich bestehen Blutkonserven aus Aufschwemmungen von größtenteils 4 Teilen Frischblut mit 1 Teil einer Stabilisatorlösung, die das Blut vor Gerinnung und Ger innselbildung schützen soll. Als Stabilisator wird am häufigsten Natrium-Zitrat mit einem Zusatz von Traubenzucker in wäßriger Lösung benutzt.It is well known that blood supplies consist of mostly suspensions 4 parts of fresh blood with 1 part of a stabilizer solution that prevents the blood from clotting and should protect clot formation. Sodium citrate is the most common stabilizer used with an addition of glucose in an aqueous solution.
In dieser Weise hergestellte und bei etwa -l-4° C aufbewahrte Blutkonserven weisen nach 14 Tagen eine zunehmende Rotfärbung im darüberstehenden, normalerweise zartgelben Plasmaanteil auf. Dieser als Hämolyse bezeichnete Vorgang kommt durch Platzen der roten Blutkörperchen zustande, deren roter Farbstoff dann in das Blutplasma übertritt. Von hämolytischen Blutkonserven wird üblicherweise kein Gebrauch mehr gemacht. Nach C u c c i o 1 i , U., Arch. ist. biochim. ital., 14, 189 (1942), treten die ersten hämolytischen Zeichen in Blutkonserven vom 15. Tage ab auf, so daß das Blut von diesem Zeitpunkt ab nicht mehr zu Transfusionen zu benutzen ist. S chürch, O., Acta soc. medic. Fenn. duodezim., B 31, 58 (1941), dagegen gibt 3 Wochen als höchste Verwendungsdauer von Blutkonserven an. Diese beschränkte Verwendungsdauer ist bei dem ständig steigenden Bedarf von erheblichem Nachteil, auch wenn man bei innerhalb dieser Zeit nicht verbrauchten Blutkonserven versucht, das über den roten Blutkörperchen stehende Blutplasma einer weiteren Verwendung zuzuführen, indem man es abpipettiert und in dieser Form zu sogenannten Plasmatransfusionen benutzt. In diesem fehlen jedoch die roten Blutkörperchen, so daß Plasmatransfusionen kein voller Ersatz der Vollbluttransfusion sein können. Neben dem Funktionsverlust ist auch die volumetrische Abnahme der Transfusionsflüssigkeit beträchtlich, da mit den Erythrocyten etwa ein Drittel bis die Hälfte des Volumens einer Blutkonserve verlorengeht.Consists of blood produced in this way and stored at around -1-4 ° C show after 14 days an increasing red color in the upper part, usually pale yellow plasma portion. This process known as hemolysis comes through The red blood cells burst, their red dye then enters the blood plasma transgresses. Hemolytic blood reserves are usually no longer used made. According to C u c c i o 1 i, U., Arch. Is. biochim. ital., 14, 189 (1942) the first haemolytic signs in blood products from the 15th day onwards, so that the Blood can no longer be used for transfusions from this point onwards. S chürch, O., Acta soc. medic. Fenn. duodezim., B 31, 58 (1941), on the other hand there are 3 weeks as maximum usage time of blood products. This limited period of use is at a considerable disadvantage with the constantly increasing demand, even if you are at within this time unused blood products tried that over the red Blood cells to supply standing blood plasma for further use by it is pipetted off and used in this form for so-called plasma transfusions. In However, this lacks the red blood cells, so that plasma transfusions do not have a full Replacement of whole blood transfusion. In addition to the loss of function is also the volumetric decrease of the transfusion liquid is considerable, since with the erythrocytes about a third to half the volume of a blood unit is lost.
Obwohl man durch Variationen des Stabilisators versucht hat, die Konservierungs- bzw. Verwendungsdauer menschlichen Blutes zu verlängern, ist es bis jetzt nicht gelungen, die maximale Grenze von 3 Wochen zu überschreiten.Although attempts have been made to reduce the preservation properties by varying the stabilizer To date, it has not been done to extend or extend the useful life of human blood managed to exceed the maximum limit of 3 weeks.
Die geschilderten Mängel werden durch die Erfindung behoben, indem dem Blut unmittelbar nach der Entnahme, d. h. dem Frischblut, Alkalisalze des Chlorophyllins in isotonischer Kochsalzlösung in einer Konzentration von etwa 0,0001 bis 2,011/o zugesetzt werden. Die so vorbehandelte Blutkonserve ist etwa zwei- bis dreimal so lange haltbar wie bisher. Selbstverständlich muß die Chlorophyllin-Verbindung bzw. die aus den Alkalisalzen des Chlorophyllins hergestellte Lösung steril filtriert und frei von Schwebestoffen sein. So kann man beispielsweise zu einer Menge von 100 ccm Frischblut, welches in bekannter Weise durch den Zusatz von 20 ccm einer 3,8%-igen Natrium-Citrat-Lösung ungerinnbar gemacht ist, ein Alkalisalz des Chlorophyllins in isotonischer Kochsalzlösung in einer Konzentration von 2% hinzufügen. Die verwendeten Alkalisalze des Chlorophyllins, wie beispielsweise Chlorophyllin-Natrium, sind ein praktisch atoxisches Naturprodukt, dem auch keine carcinogene Wirkung wie manchen anderen Farbstoffen zukommt.The deficiencies outlined are remedied by the invention by the blood immediately after collection, d. H. the fresh blood, alkali salts of chlorophyllin in isotonic saline solution at a concentration of about 0.0001 to 2.011 / o can be added. The blood reserve pretreated in this way is about two to three times as high long shelf life as before. Of course, the chlorophyllin compound or the solution made from the alkali salts of chlorophyllin is sterile filtered and be free of suspended matter. For example, you can go to a set of 100 ccm of fresh blood, which in a known manner by the addition of 20 ccm of a 3.8% sodium citrate solution is made incoagulable, an alkali salt of chlorophyllin add in isotonic saline solution at a concentration of 2%. The used Alkali salts of chlorophyllin, such as chlorophyllin sodium, are a practically atoxic natural product, which also has no carcinogenic effect like some other dyes.
Claims (2)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEP0018464 | 1957-05-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE1068862B true DE1068862B (en) | 1959-11-12 |
Family
ID=7367026
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DENDAT1068862D Pending DE1068862B (en) | 1957-05-03 | Procedure for extending the shelf life of blood products |
Country Status (3)
Country | Link |
---|---|
DE (1) | DE1068862B (en) |
FR (1) | FR1272491A (en) |
GB (1) | GB901675A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1164022B (en) * | 1960-05-31 | 1964-02-27 | Univ Minnesota | Method of preserving semen |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4082509A (en) * | 1976-08-05 | 1978-04-04 | Dow Corning Corporation | Method of storing blood and a blood storage bag therefor |
DE69432751T2 (en) * | 1993-07-05 | 2004-04-29 | Sheffield Teaching Hospitals National Health Service Trust | PREPARATION AND STABILIZATION OF CELLS |
US5858699A (en) * | 1994-04-05 | 1999-01-12 | Northern General Hospital Nhs Trust | Method to stabilize cell suspensions using aged heavy metal solution and paraformaldehyde |
-
0
- DE DENDAT1068862D patent/DE1068862B/en active Pending
-
1959
- 1959-08-19 FR FR803256A patent/FR1272491A/en not_active Expired
- 1959-08-21 GB GB28576/59A patent/GB901675A/en not_active Expired
Non-Patent Citations (1)
Title |
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None * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1164022B (en) * | 1960-05-31 | 1964-02-27 | Univ Minnesota | Method of preserving semen |
Also Published As
Publication number | Publication date |
---|---|
FR1272491A (en) | 1961-09-29 |
GB901675A (en) | 1962-07-25 |
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