DE10219924A1 - Extraction unit for cells and body fluid, applies vacuum to defined wound - Google Patents

Abstract

Following defined wounding of tissues, especially the skin layers, organs or organ layers, fluid and cellular components of the tissues are extracted using a vacuum unit (2, 10) applied to the skin (1). They are drawn into a syringe (7) for further diagnosis. An Independent claim is included for corresponding vacuum extraction equipment.

Description

An innovative method and a device for Removal of liquid and cellular tissue components using a vacuum element that is perforated or targeted injured skin surface or body surface in living and dead people and animals can be put on and for subsequent diagnostic and analytical purposes.

The normal laboratory analyzes are based on blood samples taken from the Veins performed. These blood samples from the so-called peripheral blood, however, can only capture laboratory parameters that are play systemically, i.e. throughout the body and not just locally stay limited. A focus diagnosis of fluids (more humoral) Components, as usual morphologically by biopsy or Cytologically possible for punctures, does not currently exist. With a Such humoral local diagnostics would then be possible, all so far used laboratory parameters of peripheral laboratory diagnostics also for to make local or hearth diagnostics accessible.

However, many diseases only develop locally. Especially with the well-observed diseases of dermatology, in which often no pathological laboratory parameters found in peripheral blood but clear morphological changes in the skin. That is why there are so many diseases in this medical field secured by biopsies. Especially the skin shows as a border organ special immunological phenomena that do not occur in peripheral blood are seen. In addition, there is often only a fraction of some cells stay in the peripheral blood. So 98% of the lymphocytes are located in tissue and only 2% in peripheral blood. There are also some Cell populations and inflammation products only show in tissue or Organ-specific walks.

However, biopsies that only show morphological, structural changes in the Showing the skin often fails because it has no information about the skin Fluid space or the interstitial space, the changes can give. Targeted tapping could also help certain Tissue layers targeted humoral analyzes of these tissue layers or certain organs or organ layers become.

The human body consists of over 70% liquid components. In the interstitial tissue, the connective tissue, the largest organ at all, 50-60 liters of liquid are exchanged per minute. This is a multiple of the cardiac output. In this All cells are embedded in the fluid space, since the entire cell is located above it Substance and gas transport from the capillary to the cell must take place. Changes take place very early in it, which are essential show earlier than morphological-structural changes.

Large suction cups are used in obstetrics The child's head can be put on the child from the birth canal to draw. Large suction bells are also available in erotic retailers with which the genitals can be sucked in. With these large suction bells there are no diagnostic Measures carried out.

In recent years, the so-called microdialysis z. B. Antibiotic determinations in the skin or subcutaneous fat carried out. These microdialyses are invasive, medical interventions in the skin, with tubing inserted into the tissue, the with Solutions are rinsed and the tissue water osmotically from the Pull tissue.

Various are also used to treat chronic wounds Suction devices used to z. B. fluid secretions, Suppuration, gas formation from the wounds partly through drainage suck. These devices are only for therapy, not for Diagnostics, so they are usually large-scale.

The relevant patent literature does not contain anything that the Vacuum diagnostics would be comparable. It is the example here Publication at the German Patent Office DT 26 11 721 A1 dated Filing date March 19, 1966 discussed. The inventor describes one Vacuum serum-vacuum device. The first claim discloses that a concave vacuum chamber is placed on the skin Aspirates tissue serum. The inventor expressly points out that the skin and mucous membranes should not be injured beforehand and describes the application on inner mucous membranes. The vacuum stamp explained in a drawing is elongated and connected to a vacuum motor. The disadvantages of such Equipment is on hand. For physical reasons it is e.g. B. only possible with considerably high vacuum pressures to suck out uninjured skin serum. It arises forced bruising because the vessels must be destroyed for this. In addition, the high vacuum pressures suck them out Ingredients that are also sucked through the skin layers must be traumatized in such a way that, above all, sensitive Proteins and cellular components are damaged. The one shown Vacuum stamp is designed for and for endoscopic removal the application on the skin is too unstable and its suction surface is too small.

Therapeutic, bloody cupping is from naturopathy known in which after needling the skin over a cupping glass Blood-lymphatic mixture is extracted. The cupping glass contains one Vacuum and sucks the blood-lymphatic mixture through the little ones Skin injuries to the skin surface. The extracted mixture is discarded after cupping.

In this blood-lymphatic mixture, however, apart from the humoral, liquid components also diagnostically valuable cellular components contain.

Diagnostics in naturopathy is increasingly used Dark field microscopy according to Prof. Enderlein applied the Endobionts in their various forms, growth stages and Evidence of developmental circles in the blood. These endobionts are in entire body, i.e. distributed in all tissues. So far the Dark field microscopy not for analysis of tissue fluids used.

In recent times, molecular biological and molecular genetic diagnostic methods used, the smallest Detect and identify amounts of protein or nuclear acid capital. This is especially true for the so-called extracellular protein - and nucleic acid analysis too. Such analytics can be used as recently shown a certain BSE diagnosis in animals or Detect micrometastases in humans.

In the control and monitoring of animal producers and the Delivered fresh meat encounter analytical problems every day of considerable importance. So the slaughterhouse operator knows the fresh meat processor does not care whether the delivered animal or meat are not infected with bacteria, fungi or viruses. Especially the Infested with Salmonella, Listeria, Campylobacter and Enterococci is a difficult hygienic problem due to the high Danger to humans from eating the meat is brand new. Because the animal halves or animal quarters are suspended and are bled out, an analysis with the help of blood samples is not more feasible. It would therefore be for animal surveillance and Fresh meat control of great benefit if there is a procedure or There would be instruments with which one can easily and safely without veterinary assistance from the animal or fresh meat could obtain suitable material for such investigations.

Also, it would often be useful if you didn't just have access could determine laboratory parameters for vessels, but also as before described about the removal through the skin. You needed no special anatomical knowledge. The material removal would be easier and less dangerous and the hygiene issues would be not important here. Any layman would be after brief instructions able to do this.

The task arises from the process described above and develop tools with which easy removal of all humoral and cellular components through the skin possible, which also serve as an alternative blood sample could and would be applicable to humans and animals. A procedure and So instruments that allow it, all about this removal previous laboratory parameters of the usual blood analyzes also locally and not just being able to perform systemically.

The innovation described solves this task.

According to the invention, this is achieved by using a Vacuum element with suitable accessories according to the previous one Perforation or targeted skin injury, the humoral and cellular components of the underlying tissue in the usual syringe containers of the blood collection instruments can be raised.

This removal process is vacuumed by me called. The cell and liquid mixture sucked out referred to as an extrudate. The subsequently outside the Diagnostic vacuum element could be performed as Vacuum diagnostics are referred to the type of Label sample collection, i.e. from the peripheral Delimit blood sampling.

This has the advantage that you can cook locally, in or on the stove Happened for the first time simple and comprehensive laboratory diagnostics conventional medicine and naturopathic type. In addition, many laboratory parameters that were previously only about venous or arterial vascular puncture diagnostics were also accessible can be removed through the targeted perforated or damaged skin. The extracted blood-lymph mixture, the extrudate, can after the aspirated withdrawal tapped and into the laboratory analyzers be introduced. However, you have the advantage of being able to do it all relevant skin or tissue components are hereby recorded. Moreover such a removal can always take place, since one also with unfavorable vein conditions and in infants and young children can access easily accessible skin areas.

So there is a new, always existing, alternative diagnostic access route.

To carry out such vacuum diagnostics correctly however, special suction devices, as in the patent drawings shown, used, specially adapted to the purpose are. For hygienic reasons, this is usually only done once preferred to use vacuum elements. The vacuumed However, the surface of the skin must be large, since no vessel is punctured here becomes. The extrudate can only pass through the tiny skin perforations are sucked out, leaving only one perforation small amounts of extrudate emerge.

A particular advantage of this diagnostic procedure is that that the skin in different ways for each Purpose of diagnostic vacuum extraction can be prepared. So ointments and creams can be applied to the skin before diagnosis and be removed again that numb the skin or the skin hyperaemisieren.

Another benefit is that after defined preparation diagnostic slices can be placed on the skin, their content (e.g. allergens, antibodies, markers, fluorescence markers etc.) react directly with the extrudate and after acceptance for the Prepared examinations and then can be analyzed. Also can instead of the syringe into the lumen of the Vacuum element sponges are introduced, the extrudate absorb immediately. The extrudate could later be squeezed out Sponges are obtained for analysis.

For allergy tests, this would e.g. B. mean that if the Well-defined allergens would not contain the allergen in the skin would have to be introduced, but the absorbed Extradat from the Skin immediately and natively penetrates the pane, reacts there and then could be analyzed. The patient is not at risk since nothing is in the skin is brought in.

An important advantage for dermatologists would result from the fact that these vacuum withdrawals and their subsequent analyzes, of course can also be performed on the skin diseased areas. So are exact exams of psoriasis, of eczema, of Contact allergies etc. possible, since here without local, invasive Anesthesia and sampling exact, two- and three-dimensional Samples of liquid and cellular, extractable substances from the herd can be done. These analyzes could be used in the future Routine diagnosis of skin changes is one of them. For example, if you suspect work-related contact dermatitis Inflammatory mediators and T lymphocytes extracted from the eczema skin and analyzed that only exist there. The T lymphocytes of the Contact area shows a much stronger one Sensitization to than the T lymphocytes of the peripheral blood. In addition, this stove can be much more sensitized Remove T lymphocytes, which are then z. B. about the Lymphocyte transformation test with many allergens in your Reaction could be checked. This would make eczema diagnosis significantly improve and make it safer. It also allows medication levels in the skin and Determine tissue layers such as B. of antibiotics.

This vacuum extraction of the extrudate and its subsequent one Analyzes carried out under defined conditions and with special Instrumentation is carried out, complements several established ones Diagnosis method and closes previous analytical gaps. she also enables safe diagnostics, since nothing gets into the skin is introduced, but is gently sucked out. So it will dermatological focus histology (biopsy) through this dermatological Vacuum diagnostics expanded, which naturally different analyzes can accomplish that completely elude general histology.

Allergologically, non-hazardous analyzes can be liquid and cellular skin components using the methods of established in vitro Analyzes are meaningfully combined and supplemented and thus the Make allergological diagnostics better and less dangerous.

The diagnostic vacuum extraction differs basically from the previously established morphological and laboratory diagnostic methods.

During the biopsy, cellular and connective tissue structures in the Association and in connection with a suspicious change taken. There remains a defect that is closed by a scar becomes. Local anesthesia and some suturing of the biopsy hole are usually carried out. With the punctures fluid-filled cavities, i.e. delimited structures like the bladder or punctured the belly water (ascites) and immediately the aspirate won. Or the punctures are long, thin Needle punch biopsies performed (e.g. liver or kidney). When blood is drawn normally through the veins or arteries deliberately punctured a vessel and immediately obtained the blood. In the Capillary blood collection, which is often used in pediatrics, are at defined points such as the fingertips or the Earlobes after perforation of the skin a very small amount of blood (25-200 microliters) by massaging or gently squeezing the Blood is drawn and placed in a thin capillary tube over the Capillary action sucked without vacuum.

However, vacuum diagnostics is a procedure for the slowly, under a long, constant vacuum Tissue fluid along with its cellular proportions from the Tissue is sucked out and then analyzed. Here arise no tissue defects. The analysis can be repeated as often as necessary become; Pain-free long-term analyzes are also possible. With these Long-term analyzes can be carried out with the vacuum element lying flat continuous extrudate quantities can be obtained at different times.

Such an innovative removal process and handling of the vacuum element are shown below using the example of a Skin analysis described:

The area of the skin to be analyzed is treated with an analgesic Cream and possibly cold pads pretreated. Then after Sterilization of the skin surface targeted the skin with a lancet perforated. The skin area is now stretched and this is the area Vacuum element attached. Then with the attached syringe A slight pressure creates a vacuum that attaches firmly guaranteed of the vacuum element on the skin. The airy one The interior of the vacuum elements should be in contrast to the analyzed one Skin area have a small volume to quickly and easily To be able to create a vacuum in it. The task of the vacuum element is just to seal the element airtight on the skin and not to form the reservoir for the extrudate. The extrudate is supposed to be like this be sucked into the attached analytical syringe as soon as possible, to prevent coagulation processes or other disturbing effects in the Trigger vacuum volume space. The less time and space in between, the better. The support edge of the vacuum element should be wide, however, to ensure good air exclusion. Possibly. double-sided adhesive rings that were previously on the Skin that improve this air seal to the skin and at the same time better mark the area and the vacuum element fix.

The vacuum element can also have a bulge in which the Extrudate flow and from where it can be drawn into a syringe. This bulge should be connected to the syringe through a thin tube be connected what the handling when sucking up the extrudate much simplified. All usual can be used as syringes Disposable syringes with the different interior wall coatings or Additives can be used. Of course you could also at the Make a small circle that can be punctured, through which the extrudate is aspirated through a cannula and a syringe can. At the top pole of the vacuum element, that is at the part lying at the top of the skin surface is a Connection for a syringe available, with which one can use a thin one Hose can generate the required vacuum. Of course this can also done with the analysis syringe in the bulge, or you could first use a syringe to vacuum the port generate and then suck in the extrudate. However, it is easiest via 2 syringe connections, which are provided with valves that Handling very easy and safe to make not that Load the vacuum element mechanically unnecessarily and thereby Endangering and negative pressure. If no valves are used, the thin hoses can be clamped airtight with clamps become. The use of 2 syringe accesses also has the advantage that with the vacuum syringe, if necessary, liquids (for rinsing, Coagulation and other) into the vacuum elements can without disturbing the suction of the extrudate. Is the Analysis syringe full, so the vacuum in the vacuum element be lifted and the vacuum element disposed of. in this connection due to hygiene only the usual caution as with normal Blood sampling may be necessary.

Special cases

If deeper layers of the skin are analyzed, one may have to Local anesthesia of the layer is done. You can also use targeted Diagnostics e.g. B. the fat layer used special perforation needles become. These needles can also be capillary tubes, some of them have a firm stop, which exactly limits their penetration depth. This Needles should not only define the penetration depth, but also that Keep the puncture holes open to extract the deeper To allow extrudates. By sole, short stinging perforation holes would collapse quickly and so prevent the extraction of the extrudate. The needles have that too It makes sense to get just the extrudate you want, and not that of all layers. About the little ones sticking out of the skin The vacuum element would now be slipped over and fixed in place become. Then the extrudate would be exactly as described above be removed. Special ones could also be used to keep the pores open Skin tensioner can be used. Special needles could also be used whose walls contain small holes, the more extrudate to be able to win.

Vacuum diagnostics could also be elegantly included in organ biopsies combine the punch biopsies, in which after aspiration of the Biopsates on the lying punch needle slowly building up Vacuum connects. More specific biopsy needles could be used for this be developed.

The vacuum element could be used as a set together with the syringes, Pipettes, handheld centrifuges and diagnostic tools are offered become. Possibly. could be in the set after a simple one Diagnostic disks can be integrated, which can also be used as required relevant diagostics. Or it could be in the syringe even the diagnostics are done. So could be important investigations can be carried out inexpensively by laypeople and without visiting a doctor. This would be interesting for the private sector (AaS diagnostics) and development aid would also have useful applications Find. Since there are usually no centrifuges in private households in such a set could also be a simple handheld centrifuge be incorporated. Such a centrifuge is by hand brought into rotation, in which the tightly fitting hand guides a rod, on which a holder rotatable around the rod is filled with extrudate Holds syringe in a hole. The syringe is in the Bracket hurled circularly around the rod, which after some Minutes that heavier extrudate components, so Put the cells at the bottom of the syringe and the liquid parts of the Float extrudates. Depending on the desired analysis, you could then the cellular or liquid portion of the extrudate for analysis be used. This simple handheld centrifuge could easily foldable can be added to the set.

In veterinary medicine there is now the possibility without the veterinarian Perform laboratory analyzes on animals. It will be possible Identify infections of individual animals inexpensively and thereby the Avoid mass treatment of all animals. The controls also of fresh meat is carried out by the laboratory analyzes of the tissue fluid that has been sucked out works well for the first time. Are there any special analysis tubes offered, the one allow immediate result after simple extrudate preparation, so the meat controls can be carried out very quickly and up-to-date without major Personnel and equipment costs take place in many places.

The innovative removal and the subsequent vacuum diagnostics will now be explained with reference to the following drawings.

Fig. 1 shows a variant of the vacuum element in a hemispherical design in cross section.

Fig. 2 shows the ideal variant of the vacuum element as a flat shell with spacers in cross section.

Fig. 3 shows the ideal variant of the vacuum element in supervision.

Fig. 4 shows the ideal variant of the vacuum element consisting of several chambers or of coordinated arrangements of several vacuum elements in the plan.

Fig. 5 shows the decrease of the extrudate at a hanging animal half of a cow or the fresh meat.

Fig. 6 shows the variant of the vacuum element for the removal of fresh meat in cross section.

Fig. 7 shows the variant of the vacuum element for the removal on the live animal in cross section.

In Fig. 1 adheres to the skin ( 1 ) the hemispherical vacuum element ( 2 ), which has been dotted for a better overview. It could also be bulged at one point in order to promote blood accumulation there, i.e. to serve as a reservoir. However, since the blood should expediently be drawn up immediately into the analysis syringe ( 9 ), such a variant was dispensed with. A vacuum was generated inside ( 3 ) of the vacuum element via the syringe ( 10 ) and the connecting hose ( 11 ), the syringe attachment piece ( 17 ) and the valve device ( 13 ) by moving the piston of the syringe ( 10 ) in the direction of the arrow. The vacuum element ( 2 ) has a wide support ( 7 ) on the skin, which ensures good air exclusion. This contact surface could also be self-adhesive. Due to the negative pressure, the extrudate ( 5 , 6 ) is sucked out through the perforations ( 4 ) into the interior ( 3 ) of the vacuum element. The extrudate ( 6 and 18 ) can be sucked into the analysis syringe ( 9 ) through the thin tube ( 12 ) by moving the piston of this syringe in the direction of the arrow. The analysis syringe is connected to the vacuum element via the deep-seated connection ( 14 ). The skin ( 15 ) is sucked into the interior ( 3 ) of the vacuum element in a hump-like manner by the vacuum. The height of the vacuum element must therefore be so large that the skin hump does not hit the roof of the vacuum element and thus does not hinder the extrudate from escaping. The vacuum element itself should be built as flat as possible, since it only serves to generate the vacuum and not as a collecting container for the extrudate. The extrudate should be conveyed into the analysis syringe ( 9 ) as quickly and easily as possible. Depending on the analysis, the inner wall of the vacuum element can be coated with different materials, such as the inner wall of the analytical syringe. B. be wetted with silicone or with heparin. The approach ( 13 ) of the suction syringe ( 10 ) should be established so that it does not come into contact with the extrudate. Of course, it would also be possible to work with only one syringe connection, to which the suction syringe and then the analysis syringe are connected. However, the use of 2 syringes is more practical and hygienic, since the vacuum has to be readjusted again and again during removal. A simple clamp ( 16 ) is attached to the hose of the analysis syringe, which closes the hose completely so that the syringe ( 9 ) can be removed cleanly or several syringes can be filled. The diameter of the vacuum element should be several centimeters (over 4 cm), otherwise too little extrudate will be obtained.

The advantage of vacuum diagnostics can also be clearly seen here versus capillary blood sampling at the fingertip or the earlobe.

With capillary removal, the finger pad or of the earlobe squeezing or massaging the blood out of the skin pressed. This leads to known artifacts and interference with the Blood cells. Such errors are caused by the use of negative pressure avoided. With capillary blood collection only very small Blood amounts up to a maximum of 300 microliters analyzed, while in the Vacuum diagnostics usually several milliliters of extrudate without any problems to let win.

Another advantage of the innovative process is that any layperson can carry out such extrudate extraction. For this he does not need any anatomical knowledge, he does not need any vessels dot; the withdrawal process is completely safe and the Skin surface easily accessible.

Fig. 2 shows an ideal, flat design of the vacuum element ( 20 ). It can be kept so flat because spacers ( 21 , 25 ) prevent the skin ( 15 ) from being sucked upwards. This flat vacuum element also has a wide edge seal ( 22 ) for effective air sealing. The extrudate ( 27 ) is drawn out of the skin through the perforations ( 26 ) of the skin ( 15 ). The vacuum is generated via the syringe ( 23 ), which is connected to the interior ( 3 ) of the vacuum element via the hose ( 24 ). As in Fig. 1, this hose ( 24 ) can also be connected to a valve mechanism which has been firmly integrated on the roof of the vacuum element. By means of the spacers ( 21 , 25 ), the vacuum element can be kept so flat (e.g. only 2-3 mm) that even very small amounts of the extrudate ( 27 ) in several places of the vacuum element via hoses ( 29 , 28 ) Analysis syringes ( 32 ) can be sucked up. These tubes can be brought very close to the surface of the skin due to the predetermined skin distance. Because several syringes can be attached to this vacuum element, it is also possible to introduce anticoagulant substances or other substances into the interior of the vacuum element. The analysis tubes can in turn be sealed with clamp closures ( 30 ) and carry syringe adapters or other closures ( 31 ). The spacers also serve to hold resorbing filter papers etc. on the skin surface that could be used for diagnostics. Extrudate can be removed from the vacuum element by disconnecting the individual lines with just one syringe at several points.

Fig. 3 shows the flat, round vacuum element ( 20 ) in the plan. Because of the low extrudate yield per square centimeter of skin area, the vacuum elements must cover larger areas of the skin or have a larger diameter, ideally over 4 cm, optimally 6-8 cm. Due to the possibility of removing the extrudate at various points on the vacuum element ( 24 , 29 , 28 ), the local extrudate formation can be optimally dealt with. The extrudate can also be removed at several points with a single analysis syringe. In this view, the extradate is drawn up at point ( 28 ) via the analysis syringe ( 32 ). The spacers ( 25 , 21 ) prevent the skin ( 1 ) from being lifted up. The vacuum element has the wide edge ( 22 ).

In the top view Fig. 4a shows a flat vacuum element (33), which is composed of a plurality of vacuum chambers (34) and has the shape of a dog bone. The spacers ( 35 ) are installed centrally. The analysis holes for the hoses were not shown. The flat, large-area vacuum element is flexible and transparent. FIG. 4b and 4c show honeycomb (36) and window-like (37) arrays of individual, flat vacuum elements. This guarantees that the configuration of the vacuum elements can be adapted to the purpose or size of the area to be analyzed. This could be of importance for the search of micrometastases in the course of lymph channels.

Fig. 4d shows an elongated design of a flat vacuum element, which in turn consists of several chambers ( 38 ), each containing spacers ( 39 ) and analysis tubes ( 40 ). Again, the wide edge ( 41 ) for air sealing is marked here.

Fig. 5 shows the cattle half ( 42 ), which was skinned from the hoof ( 43 ) and the hook ( 44 ) was hung. On the half of the cattle, extrudate is to be extracted from an area on the trunk ( 45 ) for infection analysis. The half of the cattle is bled out, which prevents laboratory sampling via the bloodstream. In addition, the cattle's fur was removed, so that only the surface of the muscle layer has to be damaged for the extrudate removal. The area was perforated with a special hammer ( 50 ) ( 49 ). The hammer has defined differently shaped perforation needles ( 51 , 52 ) on its impact sides. In the area ( 45 ) the skin surface was stretched using a skin tensioner. This skin tensioner has the slightly curved expansion elements ( 46 ) which are held on the skin by suction cups or clips. The two expansion elements are kept at a distance via the rods and spacer screws. Which also stretches the skin between the expansion elements. This causes the perforation holes ( 49 ) to be kept open, which facilitates the extrudate exit.

The vacuum element would be placed in the space between the expansion elements. This was not shown due to the better clarity of the picture. See Fig. 6.

Fig. 6 shows a special variant of the vacuum element in use on fresh meat. In Fig. 6 emerges from the perforations ( 49 ) in the area ( 45 ) of the extrudate ( 54 ). The extrudate follows gravity into the receptacle ( 55 ). Since the beef halves or the fresh meat hang, the vacuum element ( 56 ) can be modified especially for this application. Since large animal surfaces are available here and the animals or pieces of meat have bled, the removal area must be large (diameter 10-20 cm) in order to be able to obtain enough extrudate. A handle ( 57 ) is incorporated into this large vacuum element for better handling. The spacers ( 58 ) prevent the animal skin ( 53 ) from being pressed against the inner roof of the vacuum element. A sponge, which absorbs the extrudate, could also be introduced into the interior ( 62 ) of the element.

To remove the air from this interior, a receptacle for a larger syringe ( 60 ) is attached to the upper curvature of the vacuum element. By guiding the plunger ( 61 ) of this syringe ( 60 ) in the direction of the arrow, a vacuum is created in the interior ( 62 ) of the vacuum element ( 56 ). A vacuum compressor could also be used to remove air from the vacuum element. The vacuum element has the wide support edge ( 59 ) to ensure an airtight seal. This large vacuum element can also be constructed very stably for multiple use, as can the vacuum syringe ( 60 ). The extrudate receptacle ( 55 ) into which the extrudate flows due to gravity should be used as a disposable item. This receptacle can also be replaced by a conventional laboratory syringe into which the extrudate is drawn.

The receptacles can be kept small, because for the Infection analyzes (bacteria, fungi, viruses) only a few milliliters Extrudate are required. On average, 2 ml per analysis are sufficient. The farmer can do all of this himself without calling the vet have to. This could be an alternative today Withdrawal procedure Save costs and time. In addition, the Vacuum diagnosis of the usual blood collection via the puncture of the be superior to animal blood vessels.

The extrudate can also be used to create cultures.

Fig. 7 shows a special variant of the innovative vacuum element for use on the large live animal.

The situation here is different from that of fresh meat that is skinned and bleed, is delivered. The large farm animals (cattle, horse, Pig) have a fur and a thick leather skin. These structures must be drilled for extrudate removal in order to to get fluid muscle layer. An airtight seal the fur surface is not possible. The fur may be on the Tapping point to be shaved and disinfected. It also moves the animal during the removal procedure, so the handling is very must be carried out easily and quickly.

The handling and the vacuum element is based on a Extrudate removal from the back of a cattle explained.

The vacuum element ( 65 ) is picked up by the strong handle ( 66 ). In the thick bottom ( 71 ) of the vacuum element ( 65 ) strong special needles ( 68 ) are firmly embedded, which have holes ( 69 ) in their walls for better absorption of the extrudate. The farmer, who wants to take an extrudate sample from the animal, puts the beef in a tight box, shaves a fur spot on an easily accessible back side and with a swing sticks the vacuum element with the needles through the thick skin ( 64 ) into the muscle layer ( 63 ) of the beef saddle. The animal only feels a brief pain. Then he connects a syringe ( 72 ) to the connecting hose ( 70 ) and creates a vacuum by moving the plunger ( 73 ) of the syringe in the direction of the arrow. The animal's thick skin ( 64 ) encloses the strong needles ( 68 ). A vacuum is created in the very thin interior ( 67 ) of the vacuum element, just as in the needles, by the stamp movement. To stabilize the interior, intermediate webs ( 75 ) were incorporated between the floor and the ceiling of the vacuum element. The negative pressure pulls the extrudate ( 74 ) over the needles ( 68 ), the interior ( 67 ) of the vacuum element ( 65 ), the connecting tube ( 70 ) into the examination syringe ( 72 ). This syringe ( 72 ) can be detached from the connecting hose ( 70 ) and sent to a laboratory for analysis. However, a rough screening can also be carried out immediately on site. A change in color in the syringe could indicate a positive reaction, which could then be checked again by the veterinarian using targeted blood samples. This on-site laboratory screening could include qualitative analyzes of the most important bacterial and viral pathogens (salmonella, listeria, BSE, etc.). B. an infection would be indicated by a color change. This would enable the farmer to quickly and easily obtain rough laboratory analyzes of his animals. The same procedure could be established in the slaughterhouses. The handle ( 66 ) of the vacuum element could be designed to be removable for multiple use. This vacuum element is built very robustly to guarantee the safe puncture of the needles and their hold in the fur of the animal.

The innovation therefore describes a new process or a new one Device to easily and gently body material human and animal for diagnostic-analytical Purposes. Therapy measures are hereby not be carried out.

The vacuum element serves as a link between the Skin surface or the tissue and the analytical syringe. It enables a new diagnostic access route for extraction diagnostically relevant body materials. to Extrudate extraction is the undamaged skin, or the Body surface, minimally deliberately opened without a wound arises. The injuries immediately follow or heal quickly. There are preferably material and Analyzes of the body surfaces or close to the Body surface of located body areas.

Claims (18)

1. Removal procedure for use on living and dead people and animals, characterized in that after defined injuries to tissue layers (especially the skin layer) or organs or organ layers, humoral and cellular tissue components (extrudate) are extracted via a vacuum element placed on the skin and immediately be drawn into a syringe for further diagnosis. 2. The method according to claim 1, characterized in that all Appropriate laboratory analyzes of the usual blood samples are used are carried out including molecular genetic studies and the determination of extracellular protein and nucleic acids and of drug levels in tissues as well as analyzes of localized Tissue or organ changes (focus diagnosis) herewith be carried out and the extrudate for cultivation is being used. 3. The method according to claim 1-2, characterized in that the Procedure is used as an alternative blood collection procedure. 4. The method according to claim 1-3, characterized in that the Process for laboratory analysis of fresh meat and live meat large farm animals can be used. 5. The method according to claim 1-4, characterized in that the Tissue or organ layer with defined perforations, Milling, removal, peeling, punching, curettage, Scratches, punctures are pretreated and that means are used to the organs or tissues in their spread and depth or to meet their different depths and the Keep injuries open. 6. The method according to claim 1-5, characterized in that Means for tensioning or pulling apart the tissue or Organ layers are provided. 7. Removal device for use in the living and dead Human or animal, characterized in that after defined injuries to tissue layers or organ layers (especially the skin or the skin layers) on the Skin surfaces or organ surfaces a transparent or partially transparent vacuum element is placed on the one has a wide margin and holes in its stable wall are provided for the inlet of hoses and valves which are supplied with air from the syringe or a motor Vacuum element can be evacuated and through which Extrudate is immediately aspirated into a standard blood collection syringe can be and in the wall spacers are integrated, which protrudes the skin or organ surface more strongly into the Prevent lumens of the vacuum element and the at the Skin surface or organ surface positioned diagnostic Press media onto the skin or organ surface. 8. The device according to claim 7, characterized in that transparent or partially transparent vacuum elements of defined, appropriate sizes, shapes and vacuum developments and Single and multiple use materials can be used. 9. The device according to claim 7-8, characterized in that Vacuum elements with bulges and connections for the Collection of the extrudate and for connection of at least 2 Syringes used and one-way valves for air evacuation in the Vacuum element to be installed and permanent markings can be attached to the vacuum element with quantities. 10. The device according to claim 7-9, characterized in that for an airtight seal on the skin or organ surface the wide support edge of the vacuum element on both sides adhesive rings can be attached. 11. The device according to claim 7-10, characterized in that for targeted, defined injury to the tissue and Organ layers perforation needles, capillary tubes, hammers etc. be used that have a defined inner diameter and guarantee a defined penetration depth by being in have a stop at a defined height and if necessary in the skin or organ surface for the duration of the extraction can remain and that the perforation needles firmly in the ground of the vacuum element and be integrated Perforation needles also serve to hold the extrudate and the walls of which may have holes. 12. The device according to claims 7-11, characterized in that at the bottom of the vacuum element over the skin or Organ surface or in the receiving syringe suction plate or Dyes are installed as diagnostic discs or as Serve screening markers and defined markers, allergens, Contain antibodies, etc., which react immediately with the extrudate and can be further analyzed for diagnostic purposes and that as a recording medium for the extrudate spongy substances be used, from which the extrudate in turn with different Methods of analysis can be found. 13. The apparatus according to claim 7-12, characterized in that multiple suction devices are used, their extrudate ultimately via a common hose directly to a diagnostic Laboratory device is connected. 14. The apparatus according to claim 7-13, characterized in that several, separately evacuable in a large vacuum element and separately extractable vacuum chambers are included. 15. The apparatus according to claim 14, characterized in that these large vacuum elements of any convenient shape and size can have and are flexible. 16. The apparatus according to claim 7-15, characterized in that the vacuum element together with the associated syringes and additives (needles, tubes, etc.) in one sterile packaged set is offered for single use. 17. The apparatus according to claim 7-16, characterized in that the set is offered as a single use diagnostic set, which besides the vacuum element, syringes, hand centrifuges and Accessories include diagnostics, such as test strips, test sticks, etc. including the description of how to use the set. 18. The apparatus according to claim 7-16, characterized in that the device for the removal of body material for Determination of all diagnostic and toxicological Laboratory parameters can be used in human and veterinary medicine.