DE102019215577B3 - Device and method for wetting biological material - Google Patents

Abstract

A device for wetting biological material with at least one liquid, comprising a cassette (1) with a cassette bottom (2) and a cassette lid (3), the cassette (1) having at least two receiving areas (6) each for a holding device (7) wherein the holding device (7) serves to receive a slide (8) which carries the biological material, wherein the biological material arranged on the slide (8) can be wetted with the liquid within the closed cassette (1) and wherein the slide (8) within the closed cassette (1) can at least partially be lifted off the holding device (7) in order to allow liquid located between the specimen slide (8) and the holding device (7) to drain off. Furthermore, a method for wetting biological material with at least one liquid is specified.

Description

The invention relates to a device for wetting biological material with at least one liquid.

The invention also relates to a method for wetting biological material with at least one liquid.

Devices of the type in question are used, for example, for the automated processing of biological materials which are attached to a large number of microscopic slides and which are processed individually in incubation chambers. The main area of application is the diagnostic field of anatomical pathology.

A corresponding device is, for example, from DE 10 2016 225 885 A1 known. This describes a special holding device for a specimen slide in which the specimen slide is tilted several times during processing in accordance with a protocol developed for this purpose, at specified times and work steps in a continuous movement around the three coordinate axes. Thus, liquid that is located between the specimen slide and the holding device can drain off.

The problem with this device, however, is that the user has to open and close individual covers via a hinge on the multiblock in order to insert the specimen slide into the device. For example, in the case of an instrument with 36 individual holding devices, the user has to insert a slide 36 times individually. The advantage of this system is that a single holding device and thus the respective specimen slide always lies correctly on the temperature control unit, which ensures good temperature transfer. In addition, the suction nozzle attached in the lid is connected to the vacuum or suction unit and the gap between the holding device and the underside of the slide, in which the reagents to be sucked off collect at the lower end of the slide when it is raised. The disadvantage of this system, however, is that the user still has to do a lot of manual work by inserting the slide and after finishing the processing of the slide, just as much manual work when removing, for example, 36 individual slides. Consequently, this device is relatively time consuming to operate.

Also from the US 8 337 786 B2 a device for wetting biological material is previously known. This also has the disadvantages described above or similar.

The documents also describe US 2005/0186114 A1 , DE 102 18 988 C1 and US 2010/0031757 A1 Devices for wetting biological material with at least one liquid.

The present invention is based on the object of designing and developing a device for wetting biological material with at least one liquid of the type mentioned at the beginning in such a way that handling is facilitated with structurally simple means. Furthermore, a method for wetting biological material with at least one liquid is to be specified.

According to the invention, the above object is achieved by the features of claim 1. According to this, the device in question for wetting biological material with at least one liquid, a cassette with a cassette bottom and a cassette lid, wherein the cassette has at least two receiving areas for each holding device, wherein the holding device is used to receive a slide that holds the biological Material carries, wherein the arranged on the slide biological material inside the closed cassette is wettable with the liquid and wherein the slide inside the closed cassette can be at least partially lifted from the holding device in order to drain off liquid that is between the slide and the holding device located to enable.

A particular advantage of this configuration is that the holding devices can be arranged movably within the cassette. In particular, a corresponding design of the receiving areas of the cassette bottom and the preferably arrangement of pressure points in the cassette lid enable the holding devices to be pressed via the pressure points onto temperature control devices of a console, so that the heat can be transferred directly to the holding devices, regardless of an exact linearly shaped cassette bottom .

According to the invention, it has been recognized that the underlying object can be achieved in an amazingly simple manner by arranging several holding devices together in a cassette. In the simplest case, the cassette can have two holding areas. However, it is conceivable that the cassette can accommodate four, eight or even considerably more holding devices, for example, so that a large number of specimen slides can be handled at the same time. The “multiple system” according to the invention simplifies the entire process for the user.

The holding devices can advantageously be removed from the cassette. This allows the handling to be simplified again, since the specimen slides can first be placed on a holding device and can then be placed in the cassette together with them.

In a particularly advantageous manner, at least part of the receiving area can be formed by a passage in the cassette bottom. In other words, the cassette bottom can be designed similar to a grid, the openings serving as receiving areas for the holding devices.

According to a further advantageous embodiment, a console can be arranged, the cassette bottom being able to be placed on the console and removed from it. It is conceivable that the console has a deflection device so that each specimen slide can be lifted, preferably individually, within the closed cassette. Alternatively or additionally, a temperature control device can be arranged on the cassette and / or on the console for each receiving area. Thus, the console could be used both for temperature control, i.e. for heating or cooling, as well as for lifting the slide.

In a particularly advantageous manner, the cassette lid and the cassette bottom can be connected to one another via magnetic forces, in particular via at least one permanent magnet. Such a connection has the advantage that the cassette lid and the cassette base are precisely aligned with one another when they are brought together by the magnetic forces, so that, for example, tilting is prevented. Accordingly, such a construction is safe and easy to handle even with laboratory gloves. Alternatively or in addition, the cassette lid and the cassette base can be connected to one another in a form-fitting and / or force-fitting manner, in particular via a clamping and / or latching mechanism, for example via a bayonet lock. The advantage of a corresponding configuration is that it is simple and therefore inexpensive to manufacture. Furthermore, it is ensured that the cassette lid and the cassette base are extremely securely and firmly connected to one another.

To further simplify handling, it is conceivable that the cassette and the console can be connected to one another via magnetic forces, in particular via at least one permanent magnet. As already stated above, this has the advantage that the elements to be connected are aligned with one another, which considerably simplifies handling. In a further advantageous manner, the cassette and the console can be connected to one another in a form-fitting and / or force-fitting manner, in particular via a clamping and / or latching mechanism, for example via a bayonet lock. A secure connection between the cassette and the console can thus be realized with structurally simple means. Both in the case of a connection via magnetic forces and in the case of a form-fitting and / or force-fitting connection, the holding devices can be pressed against any temperature control devices in such a way that a uniform thermal coupling is provided.

In order to be able to remove the liquid or the reagents that are used for treating the samples located on the slide, a suction device can be arranged. This can comprise suction means arranged on the cassette lid and / or on the cassette bottom. It is conceivable that the suction means are connected to a vacuum device, for example a pump, via a preferably common fluid connection. Specifically, the fluid connection could be designed at least in some areas as a hose connection or a push-in pipe connection. In a particularly advantageous manner, pressure means could be provided in order to interrupt the fluid connection of a suction means with the negative pressure device. The push-off means could be formed by a pretensioned push-off element. The push-off means can be designed in such a way that the pressure exerted by it is only slightly above the pressure required for pushing off or squeezing off, so that the push-off means can be released with as little force as possible. For this purpose, the push-off means could be movable against its bias by a release device, for example an electromagnet or an electric motor, in order to release the fluid connection.

In a further way according to the invention, indentations are formed on the holding device and on the receiving area, on which the side wings rest. With this construction it can be achieved in a simple manner that the holding devices are movably arranged in the cassette. In a particularly advantageous manner, the depth of the indentations could be greater than the thickness of the side wings. For example, the side wings could have a thickness of 0.2 mm to 1.0 mm, in particular a thickness of 0.4 mm to 0.8 mm, preferably a thickness of 0.6 mm and the depth of the indentations in the range of 1, 1 to 1.9 mm, in particular from 1.3 mm to 1.7 mm, preferably 1.5 mm. With such a In the embodiment, pressure points could be formed on the cassette lid, which press the holding devices on the side wings in the direction of the cassette bottom when the cassette is closed. This achieves the advantage that the holding devices can be pressed onto a temperature control device when the cassette is connected to the console, even if the cassette bottom is not flat on its side facing the console, for example convex and / or concave due to the manufacturing process.

• - Einlegen von das biologische Material tragenden Objektträgern auf jeweils eine Halteeinrichtung bevor oder nachdem die Halteeirichtungen in die Aufnahmebereiche des Kassettenbodens eingelegt worden sind,
• - Zusammenführen des Kassettendeckels und des Kassettenbodens zum Schließen der Kassette,
• - Verbinden der Kassette mit der Konsole, und
• - Einbringen der Flüssigkeit und/oder der Flüssigkeiten in die Halteeinrichtungen.

With regard to the method, the underlying object is achieved by claim 19. According to this, the method for wetting biological material with at least one liquid, using a device for wetting biological material with at least one liquid, comprising a cassette with a cassette base and a cassette cover, the cassette having at least two receiving areas for one holding device each, wherein the holding device serves to receive a slide that carries the biological material, wherein the biological material arranged on the slide can be wetted with the liquid within the closed cassette and wherein the slide within the closed cassette can be at least partially lifted off the holding device in order to achieve a To enable drainage of liquid located between the slide and the holding device, or, using a device according to any one of claims 1 to 18, the following method steps:

• - Insertion of slides carrying the biological material on a holding device before or after the holding devices have been placed in the receiving areas of the cassette bottom,
• - Merging of the cassette lid and the cassette base to close the cassette,
• - Connect the cassette to the console, and
• - Introducing the liquid and / or the liquids into the holding devices.

In the manner according to the invention, it has been recognized that the wetting of biological material can be considerably simplified in that the microscope slides carrying the biological material are first placed in a cassette, which is then closed. The specimen slides can first be placed on their own holding device and these can then be placed in the holding areas of the cassette base. Alternatively, it is conceivable that the holding devices are first positioned in the holding areas and then the specimen slides are applied. Regardless of this sequence, the closed cassette equipped with the specimen slides can be connected to the console, for example inserted into it or placed on it. The liquid can then be introduced into the holding devices so that it comes into contact with the biological material.

Furthermore, additional steps can be carried out, for example heating and / or cooling the samples, moving the slides, suctioning off excess liquid, etc.

It is pointed out that the described device according to the invention can have a procedural characteristic. The features and advantages described with respect to the device can therefore expressly be part of the method according to the invention.

• 1 in einer schematischen Darstellung eine perspektivische Ansicht eines Ausführungsbeispiels einer erfindungsgemäßen Vorrichtung umfassend eine Konsole,
• 2 in einer schematischen Darstellung eine perspektivische Ansicht eines weiteren Ausführungsbeispiels einer erfindungsgemäßen Vorrichtung umfassend eine Konsole,
• 3 in einer schematischen Darstellung eine perspektivische Ansicht eines weiteren Ausführungsbeispiels einer erfindungsgemäßen Vorrichtung umfassend eine Konsole,
• 4 in einer schematischen Darstellung eine perspektivische Ansicht einer Kassette einer erfindungsgemäßen Vorrichtung,
• 5 in einer schematischen Darstellung eine perspektivische Ansicht einer Halteeinrichtung einer erfindungsgemäßen Vorrichtung,
• 6 in einer schematischen Darstellung eine perspektivische Ansicht einer weiteren Halteeinrichtung einer erfindungsgemäßen Vorrichtung,
• 7 in einer schematischen Darstellung eine perspektivische Ansicht einer weiteren Halteeinrichtung einer erfindungsgemäßen Vorrichtung,
• 8 in einer schematischen Darstellung eine perspektivische Ansicht einer weiteren Halteeinrichtung einer erfindungsgemäßen Vorrichtung,
• 9 in einer schematischen Darstellung eine perspektivische Ansicht eines Kassettenbodens einer erfindungsgemäßen Vorrichtung,
• 10 in einer schematischen Darstellung eine perspektivische Ansicht eines weiteren Kassettenbodens einer erfindungsgemäßen Vorrichtung,
• 11 in einer schematischen Darstellung eine perspektivische Ansicht eines weiteren Kassettenbodens einer erfindungsgemäßen Vorrichtung,
• 12 in einer schematischen Darstellung eine perspektivische Ansicht eines Kassettendeckels einer erfindungsgemäßen Vorrichtung,
• 13 in einer schematischen Darstellung eine perspektivische Ansicht einen Teil einer Absaugeinrichtung einer erfindungsgemäßen Vorrichtung,
• 14 in einer schematischen Darstellung eine perspektivische Ansicht einen Teil einer Absaugeinrichtung einer erfindungsgemäßen Vorrichtung,
• 15 in einer schematischen Darstellung eine perspektivische Ansicht einen Teil einer Absaugeinrichtung einer erfindungsgemäßen Vorrichtung,
• 16 in einer schematischen Darstellung eine perspektivische Ansicht eines Absaugmittels einer erfindungsgemäßen Vorrichtung,
• 17 in einer schematischen Darstellung eine weitere perspektivische Ansicht des Absaugmittels gemäß 16,
• 18 in einer schematischen Darstellung eine weitere perspektivische Ansicht des Absaugmittels gemäß 16,
• 19 in einer schematischen Darstellung eine weitere perspektivische Ansicht des Absaugmittels gemäß 16 mit einem dazu angeordneten Objektträger,
• 20 in einer schematischen Darstellung eine perspektivische Aufsicht eines Teils einer Konsole einer erfindungsgemäßen Vorrichtung,
• 21 in einer weiteren schematischen Darstellung eine perspektivische Unteransicht des Teils einer Konsole gemäß 20,
• 22 jeweils in einer schematischen Darstellung eine perspektivische Ansicht einer Auslenkeinrichtung sowie einer Auslenkeinrichtung mit aufgelegtem Objektträger einer erfindungsgemäßen Vorrichtung,
• 23 in einer schematischen Darstellung eine perspektivische Ansicht der Auslenkeinrichtung einer erfindungsgemäßen Vorrichtung,
• 24 in einer schematischen Darstellung eine perspektivische Ansicht eines Kassettendeckels, zur Verwendung mit der Auslenkeinrichtung gemäß den 22 und 23,
• 25 in einer schematischen Darstellung eine perspektivische Ansicht eine Aufnahme für eine Temperiereinrichtung einer erfindungsgemäßen Vorrichtung,
• 26 in einer schematischen Darstellung eine perspektivische Ansicht einer weiteren Kassette einer erfindungsgemäßen Vorrichtung,
• 27 in einer schematischen Darstellung eine perspektivische Ansicht einen Teil einer Absaugeinrichtung einer erfindungsgemäßen Vorrichtung, 28 in einer schematischen Darstellung eine perspektivische Ansicht den Teil einer Absaugeinrichtung gemäß 27,
• 29 in einer schematischen Darstellung eine perspektivische Ansicht eines Absaugmittels einer erfindungsgemäßen Vorrichtung,
• 30 in einer schematischen Darstellung eine weitere perspektivische Ansicht des Absaugmittels gemäß 29,
• 31 in einer schematischen Darstellung eine weitere perspektivische Ansicht des Absaugmittels gemäß 29, und
• 32 ein Blockdiagramm zur Darstellung eines Ausführungsbeispiels des erfindungsgemäßen Verfahrens..

There are now various possibilities for designing and developing the teaching of the present invention in an advantageous manner. For this purpose, on the one hand, reference is made to the claims subordinate to claim 1 and, on the other hand, to the following explanation of preferred exemplary embodiments of the invention with reference to the drawing. In connection with the explanation of the preferred exemplary embodiments of the invention with reference to the drawing, generally preferred embodiments and developments of the teaching are also explained. Show in the drawing

• 1 in a schematic representation a perspective view of an embodiment of a device according to the invention comprising a console,
• 2 in a schematic representation, a perspective view of a further exemplary embodiment of a device according to the invention comprising a console,
• 3 in a schematic representation, a perspective view of a further exemplary embodiment of a device according to the invention comprising a console,
• 4th in a schematic representation a perspective view of a cassette of a device according to the invention,
• 5 in a schematic representation, a perspective view of a holding device of a device according to the invention,
• 6th in a schematic representation, a perspective view of a further holding device of a device according to the invention,
• 7th in a schematic representation, a perspective view of a further holding device of a device according to the invention,
• 8th in a schematic representation, a perspective view of a further holding device of a device according to the invention,
• 9 in a schematic representation a perspective view of a cassette bottom of a device according to the invention,
• 10 in a schematic representation a perspective view of a further cassette base of a device according to the invention,
• 11 in a schematic representation a perspective view of a further cassette base of a device according to the invention,
• 12th in a schematic representation a perspective view of a cassette lid of a device according to the invention,
• 13 in a schematic representation, a perspective view of part of a suction device of a device according to the invention,
• 14th in a schematic representation, a perspective view of part of a suction device of a device according to the invention,
• 15th in a schematic representation, a perspective view of part of a suction device of a device according to the invention,
• 16 in a schematic representation a perspective view of a suction means of a device according to the invention,
• 17th in a schematic representation a further perspective view of the suction means according to FIG 16 ,
• 18th in a schematic representation a further perspective view of the suction means according to FIG 16 ,
• 19th in a schematic representation a further perspective view of the suction means according to FIG 16 with a slide arranged for this purpose,
• 20th in a schematic representation a perspective top view of part of a console of a device according to the invention,
• 21st in a further schematic illustration a perspective view from below of the part of a console according to FIG 20th ,
• 22nd each in a schematic representation a perspective view of a deflecting device and a deflecting device with an attached slide of a device according to the invention,
• 23 in a schematic representation a perspective view of the deflection device of a device according to the invention,
• 24 in a schematic representation a perspective view of a cassette cover for use with the deflection device according to FIG 22nd and 23 ,
• 25th in a schematic representation a perspective view of a receptacle for a temperature control device of a device according to the invention,
• 26th in a schematic representation a perspective view of a further cassette of a device according to the invention,
• 27 in a schematic representation, a perspective view of part of a suction device of a device according to the invention, 28 in a schematic representation a perspective view of the part of a suction device according to FIG 27 ,
• 29 in a schematic representation a perspective view of a suction means of a device according to the invention,
• 30th in a schematic representation a further perspective view of the suction means according to FIG 29 ,
• 31 in a schematic representation a further perspective view of the suction means according to FIG 29 , and
• 32 a block diagram to illustrate an embodiment of the method according to the invention ..

In the figures, the same elements are provided with the same reference symbols. Furthermore, in order to improve clarity, not all elements are provided with a reference number in every figure.

The 1 and 2 each show in a schematic representation a perspective view of an exemplary embodiment of a device according to the invention. This has a cassette 1 consisting of a cassette bottom 2 and a cassette lid 3 on. In 1 is only the preferably transparent cover plate to simplify the illustration 4th of the cassette lid 3 shown.

The cassette 1 is on the console 5 arranged and has a total of four receiving areas 6th for one holding device each 7th on. On the holding devices 7th is one slide each 8th hung up.

The console 5 has in a first embodiment according to 1 a first record 9 and a second plate 10 which are designed as individual components. In a second embodiment according to 2 are the first record 9 and the second plate 10 designed in one piece, for example fused together. For the sake of simplicity, the following is still the first plate 9 and the second plate 10 even if it can be a single plate.

It can be clearly seen that the first plate 9 is formed obliquely. The slope can be in the range from 1.5 ° to 5 °, in particular from 2 ° to 4 °, preferably 3 °. The oblique course of the first plate 9 can be designed independently of the further construction of the device. It is thereby achieved that the liquid in the holding device 7th is pipetted into it, in the direction of the one in the bottom of the cassette 2 trained overflow channel 11 running. Furthermore, the incline enables any bubbles in the liquid to rise better.

There are also four electric motors 12th designed as a drive for a deflection device 13 serve over which the slides 8th in the cassette 1 can be deflected. In addition, there is the suction device 14th to recognize who the suction means 15th includes, over which the liquid from the individual holding devices 7th can be extracted. The suction means are for this purpose 15th via a fluid connection 16 for example connected to a pump or another device for generating a negative pressure. The fluid connection 16 is partially designed as a hose connection. To interrupt the hose connection, pressure release agents are required 17th provided the electromagnet here 18th include, any other adjusting elements are conceivable. There are also tax funds 19th , for example electronic boards, arranged.

In the embodiment shown here, the cassette 1 and the console 5 via magnets, not shown 20th connected with each other. Furthermore are the cassette floor 2 and the cassette lid 3 about magnets 21st connected with each other.

3 shows in a schematic representation a perspective view of a further embodiment of a device according to the invention. The device generally corresponds to that in FIGS 1 and 2 Device shown, so that reference is made to the relevant description. The main difference is that the cassette bottom 2 and the cassette lid 3 form and force fit, namely via a first positioning hook 60 , which acts in the sense of a bayonet lock, are connected to one another. The moreover one is the cassette 2 via two second positioning hooks 61 , which act as locking hooks, form and force fit with the console 5 connected.

Another difference is that the suction means 15th each in a corner of the holding devices 7th are positioned.

4th shows a schematic representation of a perspective view of a cassette 1 a device according to the invention. The cassette 1 has multiple recording areas 6th in which suction means designed as suction nozzles 15th are arranged. The cassette bottom 2 has the functions of “picking up, positioning and holding” a defined number of holding devices 7th on. Furthermore, the cassette bottom serves due to its in 4th undetectable magnets 20th for automatic positioning to a console 5 .

The cassette lid 3 has the functions of “absorbing and holding a certain number of suction agents 15th or suction nozzle "according to the number of holding devices 7th in the cassette bottom 2 on. Furthermore, through the pressure points 34 of the cassette lid 3 those with side wings 22nd trained holding devices 7th that are loose in the receiving areas 6th are hung or stored, in the intended and recessed positioning points of the cassette floor 2 pressed. Possibly. serves the cassette lid 3 at the same time for pressing the bottom of the holding devices 7th on the individual temperature control devices 23 if these are arranged. In addition, the cassette lid 3 with a cover disc 4th be covered, which can be transparent or partially transparent in order to reduce the partial evaporation of the same resulting from the heating of the reagents.

The cassette 2 is closed after the slide to be processed 8th outside the device in the holding devices 7th have been inserted. “Close” means that the cassette lid 3 on the cassette bottom 2 is placed and then by the magnetic pulling forces of the magnets 21st in both subcomponents, automatically position themselves in relation to each other and at the same time close with magnetic force. Alternatively, the microscope slide 8th also in the already closed cassette 2 be inserted from above when the cover disc 4th of the cassette lid 3 has been removed. This is a realistic option and can be determined by the user. The immune staining process is not affected.

After the staining process is completed, the cassette 2 from the console 5 removed. Removing means that the forces of the magnets 20th that is in the console 5 and in the cassette 1 e.g. in the longitudinal struts 24 are attached, must be overcome by manual or mechanical gripping. After that the cassette 1 consisting of opening the cassette lid 3 from the cassette bottom 2 is manually disconnected, thus reducing the forces of the magnets 21st in cassette lid 3 and cassette bottom 2 must be overcome in terms of strength. The microscope slide 8th can then be removed with or without holding devices. When closed and with a cover disc 4th equipped, can hold a slide 8th that is only loose in the holding device 7th is not out of the cassette, even if it overturned 1 fall.
The 5 to 8th each show in a schematic representation a perspective view of different holding devices 7th a device according to the invention.

At the holding device 7th it is one of the central components of the device according to the invention. For this reason, it is advantageous to have a holding device 7th that enables easy handling and at the same time meets the requirements demanded by the specialist staff who assess the immunostaining. These requirements include: as little background noise as possible, high specificity, repeatability, uniqueness. The ones in the 5 to 8th described holding devices 7th make it possible not only to meet these requirements, but also allow the use of holding devices 7th different sizes, so that an adaptability to the size of a slide 8th but also an adaptation to the size of the staining area on the slide 8th is created. With a short holding device 7th , for example only 30 mm long instead of the usual 55 mm to 60 mm, the reagent consumption is automatically limited. This will only take about half of the slide 8th wetted with reagents. The biological material to be stained must therefore be placed on the front, wettable surface of the slide 8th , be attached. Regarding the consumption of reagents, it is important that the slide 8th not the side walls 26th a holding device 7th touches in order to avoid that by capillary but also by other forces of surface tension reagents in the lateral gaps between the inside of a side wall 26th and the sides of the slide 8th crawl.

For this reason, the 5 to 8th various holding devices 7th each shown different concepts of spacers 25th include.

At the in 5 holding device shown 7th run the side walls 26th oblique and have elongated side wings 22nd that are used for positioning. Furthermore, there are elongated or punctiform, embossed support shoulders 27 on the back wall 28 as well as a support point 29 at the tear-off edge 30th for the microscope slide 8th educated.

In the 6th shown holding device 7th has sloping side walls 26th on, in contrast to the holding device after 5 no side wings 22nd available. There are also side spacers 25th on the two inner sides of the holding device 7th educated. There are also support points to hold the slides 29 provided, which can be implemented as punctual impressions in the ground. The suction of the liquid is ideally done from one of the two corners on the rear wall 28 .

In the 7th shown holding device 7th has sloping side walls 26th on, in contrast to the holding device after 5 no side wings either 22nd available. Furthermore, there are only a single side spacer and only a single rear wall spacer 25th arranged. Inside the holding device 7th are the support point in the corner provided for suctioning off the liquid 29 formed on the bottom, in particular embossed in this.

In the 8th shown holding device 7th has at least substantially vertical side walls 26th on, ie the side walls 26th run essentially at an angle of 90 ° with respect to the bottom of the holding device 7th . Furthermore, there are relatively short side wings 22nd on the side walls 26th as well as bulges 31 intended. The bulges 31 serve for pipetting in or for inserting a pipetting needle. In this embodiment, the liquid is sucked off in the middle or from a corner.

The 9 to 11 show different configurations of a cassette bottom 2 .

• - Positionieren der Halteeinrichtungen 7 im Kassettenboden,
• - Integration der Überlaufrinne 11, die zugleich ein Abstandshalter zum Gehäuse der Auslenkeinrichtung 13 ist
• - Gehäuse und Aushöhlung für die Auslenkeinrichtung 13 bzw. für dessen Spitze pro Halteeinrichtung 7,
• - Aufnahme von Magneten 20, 21 mit den drei Funktionen
  1. 1. Positionieren des Kassettenboden 2 mit den Halteeinrichtungen 7 an der Konsole 5,
  2. 2. Verschließen des Kassettenboden 2 mit dem Kassettendeckel 3, und
  3. 3. Positionieren der Kassette 1 in der Konsole 5. jeweils durch Wirksamwerden der Magnetkräfte mit korrespondierenden Magneten in den anderen Teilkomponenten.
• - Individuelles Auflegen der im Kassettenboden 2 leicht beweglich gelagerten Halteeinrichtungen 7 auf die Temperiereinrichtung 23

The concept of the cassette floor 2 integrates the functions:

• - Positioning the holding devices 7th in the cassette bottom,
• - Integration of the overflow channel 11 , which is also a spacer to the housing of the deflection device 13 is
• - Housing and cavity for the deflection device 13 or for its tip per holding device 7th ,
• - Magnetic inclusion 20th , 21st with the three functions
  1. 1. Positioning the cassette bottom 2 with the holding devices 7th at the console 5 ,
  2. 2. Close the cassette bottom 2 with the cassette lid 3 , and
  3. 3. Position the cassette 1 in the console 5 . in each case by the effect of the magnetic forces with corresponding magnets in the other subcomponents.
• - Individual placement of the in the cassette floor 2 easily moveable holding devices 7th on the temperature control device 23

The in 9 illustrated cassette bottom 2 is adapted to the in 5 Holding device shown 7th . There are indentations 32 in / on the longitudinal struts 24 for positioning a holding device in the xy plane 7th by means of the elongated side wings 22nd intended. The depth of the indentations can be advantageous 32 be greater than the thickness of the side wings 22nd so that the holding devices are movable within the receiving areas 6th are stored. This can result in the bottom of the cassette bottom not being perfectly flat 2 be balanced so that the holding devices 7th on any temperature control device 23 the console 5 essentially completely supported. The holding devices 7th on their side wings 22nd of pressure points 34 of the cassette lid 3 towards the bottom of the cassette 2 be pressed. Furthermore are the slopes 33 in the longitudinal struts 24 adapted to the sloping side walls 26th the holding device 7th according to 5 . The indentations 32 also serve as pressure-absorbing surfaces for the cassette lid 3 with its pressure point 34 exercises. Furthermore, the overflow channel 11 as well as a deepening 35 shown. Into the recess 35 a corresponding counterpart of the cassette lid engages 3 so that the two parts are positioned or aligned with one another. It should be noted that the recording areas 6th are formed by recesses, so that the bottom of the holding devices 7th is not covered by the cassette bottom.

The in 10 illustrated cassette bottom 2 is on both the in 6th Holding device shown 7th as well as the in 7th Holding device shown 7th customized. They are used in the longitudinal struts 24 trained bevels 33 as a positioning aid and prevent the holding devices from slipping through the receiving areas 6th . The positioning of a holding device 7th can be variable in the longitudinal plane. The suction can come from one of the two corners of the holding device 7th respectively.

The in 11 illustrated cassette bottom 2 is adapted to the in 8th Holding device shown 7th . There are indentations 32 in / on the longitudinal struts 24 for positioning a holding device in the xy plane 7th by means of the side wings 22nd educated. The indentations 32 are the respective side wings 22nd adapted in such a way that it also acts as the pressure-receiving surface of the cassette lid 3 serve. There are also additional indentations 36 at the points of the longitudinal struts 24 provided on which in the holding device 7th left and right bulges 31 for a pipetting needle are available. The depth of the indentations can be advantageous 32 be greater than the thickness of the side wings 22nd so that the holding devices are movable within the receiving areas 6th are stored. This can result in the bottom of the cassette bottom not being perfectly flat 2 be balanced so that the holding devices 7th on any temperature control device 23 the console 5 essentially completely supported. The holding devices 7th on their side wings 22nd of pressure points 34 of the cassette lid 3 towards the bottom of the cassette 2 be pressed.

• - Ausüben eines Drucks auf die Seitenflügel 22 der Halteeinrichtungen 7 mittels eines insbesondere weichen und/oder flexiblen Druckpunktes 34. Dieser kann vorzugsweise aus Silikon oder Gummi hergestellt sein und/oder durch eine Feder, insbesondere eine Druckfeder realisiert sein, und in/an den Deckelstreben 37 angeordnet sein. Damit werden die beiden Seitenflügel 22 einerseits in die Einbuchtung 32 Kassettenboden 2 eingedrückt und andererseits der Boden einer jeden Halteeinrichtung 7 individuell auf die Temperiereinrichtungen 23 gedrückt.
• - Aufnehmen und Festhalten der Absaugmittel 15 bzw. Absaugstutzen
• - Aufnahme einer insbesondere transparenten oder teil-transparenten Deckelscheibe 4
• - Aufnahme einer Positionierschraube oder eines Positionierstifts um einen Objektträger 8 in einer Halteeinrichtung 7 um ihre Längsachse drehen zu können (für Absaugungen der Flüssigkeit in einer Ecke).

12th shows a schematic representation of a perspective view of a cassette lid 3 a device according to the invention, in particular that of the cassette bottom 2 facing side of the cassette lid 3 . The cassette lid 3 integrates the functions:

• - Applying pressure to the side wings 22nd the holding devices 7th by means of a particularly soft and / or flexible pressure point 34 . This can preferably be made of silicone or rubber and / or implemented by a spring, in particular a compression spring, and in / on the cover struts 37 be arranged. This will make the two side wings 22nd on the one hand in the indentation 32 Cassette bottom 2 pressed in and on the other hand the bottom of each holding device 7th individually on the temperature control devices 23 pressed.
• - Picking up and holding the suction means 15th or suction nozzle
• - Receiving an in particular transparent or partially transparent cover plate 4
• - Acceptance of a positioning screw or a positioning pin around a slide 8th in a holding device 7th in order to be able to rotate its longitudinal axis (for suction of the liquid in a corner).

The 13 to 19th show the individual components of a suction device 14th a device according to the invention.

In 13 are the pressing agents 17th as well as the suction means 15th shown. The impression agents 17th serve a deformable hose 16 or a deformable tube by squeezing complete from the vacuum suction. This allows a vacuum valve to be replaced, which is more expensive and complicated to manufacture. However, the invention also allows the use of a vacuum valve.

The impression means shown here 14th show you as a lever 41 formed push-off element 14th on, which is anchored at one end by means of a pin. The other end of the lever 41 is for example with the anchor 42 an electromagnet 18th connected. For example on the underside of the lever 41 , just before anchor 42 , for example, is a compression spring 43 , alternatively also a tension spring, attached to the lever 41 constantly pushing up (or alternatively in one direction). Above the lever 41 A deformable piece of tubing is located, preferably at a 90 ° angle 16 or piece of pipe, which from the lever 41 should be squeezed. For this it is necessary if on the underside of the deformable hose 16 / Rohrs the lever 41 on the opposite side of the hose 16 - in the case described - above to attach a device causing a counterpressure. There is a lid for this 44 for the housing 45 the suction device 14th provided that a ledge 46 or has a nose that is applied to the deformable tube from above 16 or the tube pushes and the upper side of the hose 16 slightly squeezes down when idle. The lever that pushes up from the bottom 41 thus closes the hose 16 constantly at rest and is activated by activating the electromagnet 18th , making the lever 41 is pulled down, opens. Ideally the compression spring 43 slightly stronger in pressure than the resistance of the deformable hose 16 or of the tube, which is a relatively small or weak electromagnet 18th suffices around the hose 16 to open and to enable suction using a vacuum.

An alternative or additional means of impression 17th is in the 27 and 28 shown. This has an engine 38 , for example an electric motor 38 , to raise or lower a lever 41 , a bracket 39 to mount the motor 38 on a plate 9 , 10 and a static or movable abutment 40 on. The motor 38 can be threaded onto the lever 41 act so that this is caused by a rotary movement of the motor 38 can be moved linearly. By one of the engine 38 induced rotation, for example a left turn, can be the lever 41 extended and squeezes the flexible hose 16 against the abutment 40 . In this way, in the completely squeezed state, the possibly continuously acting vacuum is blocked and unwanted suction is prevented. Will the lever 41 retracted by by the engine 38 a reverse rotation is initiated, for example a clockwise rotation, the hose opens 16 in its original form and the vacuum comes into effect for suction.

• - Aufnahme und Befestigung des Hebels 41 der Abdrückmittel 41,
• - Aufnahme der seitlichen, verformbaren Röhre 16 der Absaugeinrichtung 14,
• - Bereitstellen eines Durchgangs 47 zur Aufnahme und zum dichtem Abschließen des Auslasses der als Absaugstutzen realisierten Absaugmittel 15,
• - Aufnahme der Druckfeder/n 43, alternativ auch durch Konstruktionserweiterung der Zugfeder/n,
• - Befestigung des als Absaugstange ausgebildeten Teils der Fluidverbindung 16 an der Rückseite,
• - Aufnahme in einer schachtähnlichen Aushöhlung.

That in the 14th and 15th housing shown 45 the suction device 14th together with the cover that closes the housing 44 (not shown here) includes the functions:

• - Mounting and fastening of the lever 41 the impression agent 41 ,
• - Reception of the lateral, deformable tube 16 the suction device 14th ,
• - Providing a passage 47 for receiving and sealing off the outlet of the suction means implemented as suction nozzles 15th ,
• - Admission of the compression spring / s 43, alternatively also by expanding the construction of the tension spring / s,
• - Attachment of the part of the fluid connection designed as a suction rod 16 at the back,
• - Shot in a shaft-like cavity.

In the 14th and 15th is shown that the housing 45 a recording 48 for the anchor 42 of the electromagnet 18th as well as the other components of the impression means 17th and the fluid connection 16 having.

• - Unterdruckeinrichtung,
• - ggf. Reagenzien-Auffanggefäß(e),
• - quer liegende Hauptröhre 16,
• - Abdrückmittel 17 an den seitlichen, verformbaren Absaugröhren 16 bzw. Schläuchen 16 über den Absaugmitteln 15 bzw. Absaugstutzen mit den abzusaugenden Reagenzien.

The 16 to 19th show the suction means implemented as suction nozzles 15th a device according to the invention.
The sub-component of the suction means designed as a suction nozzle or suction lip 15th represents the last sub-component of the entire suction device, consisting of

• - vacuum device,
• - if necessary, reagent collecting vessel (s),
• - transverse main tube 16 ,
• - Impression agents 17th on the side, deformable suction tubes 16 or hoses 16 above the suction means 15th or suction nozzle with the reagents to be suctioned off.

• Gemäß einer ersten Ausführungsform werden die gebrauchten Reagenzien in der Mitte des nur angehobenen aber nicht über die Längsachse gedrehten Objektträgers 8 abgesaugt und in einer zweiten Version über eine Ecke des Objektträgers 8. Diese zweite Version ist bereits in DE 10 2016 225 885 A1 beschrieben, wobei auf deren diesbezügliche Offenbarung, insbesondere die Absätze [0077], [0078] und [0084] sowie 1 ausdrücklich Bezug genommen wird und diese einen Teil der hier vorliegenden Offenbarung darstellen.

In order to ensure a safe and reliable, complete suction of the reagents from the reagent gap, a special design of the suction nozzle is required 15th . In particular, three formats of such a suction nozzle are conceivable 15:

• According to a first embodiment, the reagents used are in the middle of the slide which has only been raised but not rotated about the longitudinal axis 8th sucked off and in a second version over a corner of the Slide 8th . This second version is already in DE 10 2016 225 885 A1 described, with reference to the relevant disclosure, in particular paragraphs [0077], [0078] and [0084] and 1 expressly incorporated by reference and these form part of the disclosure herein.

• Eine Stufenlippe 50 sollte die Rückseite des in ungefähr 0,1 mm Höhe auf den beiden Abstandshaltern 27, 29 liegenden Objektträgers 8 berühren. Die gesamte Höhe vom Boden einer Halteeinrichtung 7 und der oberen Fläche eines Objektträgers 8 beläuft sich vorzugsweise, definiert durch die Höhe der Abstandshalter 27, 29 und der Dicke des Objektträgers 8, auf ca. 1,1 mm. Eine nach vorne ausgerichtete, beispielsweise ca. 1-3 mm lange Stufenlippe 50 überragt die Rückseite des Objektträgers 8 und berührt dessen Oberseite leicht. Durch dieses direkte Anlehnen der vorderen Wand der Stufenlippe 50 an die Rückseite des Objektträgers 8 entsteht eine dichte Verbindung durch die der Vakuumsog direkt an den Reagenzien-Spalt weitergeleitet wird. Die Stufenlippe 50 berührt den Boden der Halteeinrichtung 7 nicht, sondern endet in einer Distanz vom Boden der Halteeinrichtung 7 von vorzugsweise mindestens 0,1 mm Höhe und entspricht damit der Höhe der Abstandshalter 27, 29 und insbesondere maximal 1,0 mm über dem Boden der Halteeinrichtung 7, also ca. 0,1 mm bevor die obere Kante des Objektträgers 8 an dessen Rückseite endet. Ein normal normierter Objektträger 8 weist eine Stärke von 1,0 mm auf, so dass die gesamte Höhe bestehend aus Abstandshalter 27, 29 und Oberseite des Objektträger 8 insbesondere 1,1 mm beträgt.

The ones in the 16 to 19th shown first embodiment A relating to a central suction nozzle 15th has a gap at the bottom 49 on that through the connection opening 63 and another fluid connection 16 can be coupled to a vacuum device and the vacuum suction to the end of the slide 8th diverts that the reagents can be completely sucked out of the opened reagent gap in an angled state. The complete suction of a used reagent is the decisive feature, because this is the only way to ensure that there is no mixing with the subsequent reagent. To ensure this, the following design and process features are particularly advantageous:

• A step lip 50 the back of the should be about 0.1mm high on the two spacers 27 , 29 lying slide 8th touch. The total height from the bottom of a holding device 7th and the top surface of a slide 8th is preferably defined by the height of the spacers 27 , 29 and the thickness of the slide 8th , to approx. 1.1 mm. A stepped lip that is oriented towards the front, for example approx. 1-3 mm long 50 protrudes beyond the back of the slide 8th and lightly touches its top. By directly leaning against the front wall of the step lip 50 to the back of the slide 8th creates a tight connection through which the vacuum suction is passed directly to the reagent gap. The step lip 50 touches the bottom of the holding device 7th not, but ends at a distance from the bottom of the holding device 7th of preferably at least 0.1 mm in height and thus corresponds to the height of the spacers 27 , 29 and in particular a maximum of 1.0 mm above the bottom of the holding device 7th , i.e. approx. 0.1 mm before the upper edge of the slide 8th ends at the back. A normal standardized slide 8th has a thickness of 1.0 mm, so that the entire height consists of spacers 27 , 29 and top of the slide 8th in particular is 1.1 mm.

Especially in 19th is the part of the fluid connection 16 You can clearly see where the reagents are from the step lip 50 be sucked in.

In the 29 to 31 is an alternative version B of a suction device 15th shown. Contrary to embodiment A ( 16 to 19th ) no step lip 50 with a crack 49 on, but an at least substantially round suction opening 62 . The suction opening 62 can through the connection opening 63 and a fluid connection 16 be coupled with a suction device. The suction opening 62 can in the aspirator 15th be shaped or formed for example by the end of a hose, for example a silicone hose. The suction from the gap between the holding device 7th and microscope slide 8th works extremely reliably when the suction opening 61 has an outside diameter of 5 mm ± 2 mm and an inside diameter in the range from 2 mm to 4 mm, preferably from 3 mm to 3.5 mm. In this case, the suction opening is also advantageously 62 with no more than half their internal diameter on the slide 8th is placed so that at least the other half of the suction opening 62 behind the slide 8th Remains “free”. The suction created by the vacuum described above “pulls” the used reagents out of the reagent gap in an excellent way.

Furthermore, the 16 to 19th as well as in the 29 to 31 shown suction means 15th or suction nozzle optionally a filling passage 64 have, for example, a liquid through this filling passage 64 into the holding device 7th bring in.

The 20th and 21st show in a schematic representation a perspective view of the first plate 9 a console 5 a device according to the invention. It is pointed out again that the first plate 9 and the second plate 10 can be formed in one piece.

The following is the first plate 9 as a base plate 9 denotes and is used to attach several technical components. Each holding device is 7th a base plate 9 assigned. There is an electromagnet at the bottom 18th whose function is to open and close the hose connection 16 the suction device 14th is an electric motor 12th with pestle 51 , the one for raising and lowering the slide 8th is needed the control funds 19th and a fastening option for the temperature control device 23 that are at the top of the base plate 9 attached, attached. It is essential that on the top of the base plate 9 a positioning aid 52 is formed into a corresponding geometry on the cassette bottom 2 engages so that the two elements are positioned in alignment with one another.

At the top of the base plate 9 becomes at one end, the rear end, the housing 45 the suction device 14th attached by the anchor 42 of the electromagnet 18th the impression agents 40 who have favourited the deformable hose 16 continuously compresses and is only opened for suction when necessary. Centered on the top of the base plate 9 becomes the temperature control device 23 attached, and at the other end, the front end, the end of the plunger protrudes 51 of the motor 12th with step rocker 54 or another end piece. At the in 21st shown underside of the base plate 9 are magnets 20th provided that with the magnets 20th of the cassette bottom 2 work together.

On both sides of the temperature control device 23 and in the further front area of the base plates 9 can the cassette bottom 2 to be ordered.

22nd each shows in a schematic representation a perspective view of a deflection device 13 as well as a deflection device 13 with slide on top 8th a device according to the invention.

A slide 8th is resting on the spacer 27 , 29 resting parallel to the ground in a holding device 7th . In the gap, for example approx. 0.1 mm high, between the top of the bottom of the holding device 7th and the bottom of the slide 8th with attached biological material (tissue section, cells, smears, etc.) are the reagents required for processing, which must be exchanged depending on the procedure protocol. For an accelerated biochemical reaction it can be advantageous to mix a reagent in the gap. This can be done by raising and lowering the slide 8th can be carried out without suction. To exchange a used reagent with a new reagent, the slide 8th raised and if you stay briefly in the raised position by activating the suction device 14th sucked off. The new reagent is then placed back in the holding device 7th pipetted into it and the slide 8th lowered again. Alternatively, pipetting in can also be performed in a lowered position, parallel to the spacer 27 , 29 of the bottom of the holding device 7th be carried out, since the capillary forces in the capillary gap created by the lowering of the reagents, regardless of the pipetting point around a slide 8th , soaks in.

In the present invention it is essential that a slide 8th from the "angled position" again completely parallel to the spacers 27 , 29 of the bottom of the holding device 7th can be lowered. It is therefore a special deflection device 13 necessary for raising and lowering, which guarantees that a slide 8th is lowered precisely with its own weight of just a few grams.
The ones in the 22nd and 23 deflection device shown 13 has the peculiarity of being slightly out of the side wall of the cassette bottom 2 in the recording area 6th sticking out to prevent the front edge 53 of the slide 8th can get stuck on the frame wall. The force to move the slide 8th (Raising, lowering, rotating) can be done by an electric motor 12th , an electromagnet, an air or water pressure device, a hydraulic device, a spring with a return device, etc. can be generated. In 22nd is the ram 51 of an engine 12th to recognize on which a step seesaw 54 is pivotably arranged. The step seesaw 54 touches the slide 8th , lifts this up and tilts forward. Thus the slide becomes 8th towards the aspirator 15th pressed.

In 23 is the effect described above that the step rocker 54 can be inclined in the direction of the suction side. For this it is advantageous if the inner wall 58 of the cassette bottom 2 at the points where the step rocker is moved by extending the ram 51 "Rubs", is designed at an angle. A corresponding design of the cassette bottom 2 is in 24 shown. Inside are the slopes 59 to support the movement of the step seesaw 54 clearly visible.

1. a) Ein Aluminiumplättchen mit daran befestigter Widerstandsheizung, das durch Umgebungstemperatur passiv abgekühlt wird.
2. b) Ein Aluminiumplättchen mit daran befestigter Widerstandsheizung, das durch eine aktive Luftkühlung abgekühlt wird bspw. mit einem an den Außenseiten des Aluminiumplättchens vorbeigeleiteter Luftstrom, welcher der Einfachheit halber aus der normalerweise bereits gekühlten Luft im Labor entnommen wird.
3. c) Ein Termo-Elektromodul (Peltierelement) das aktiv heizt und kühlt.
4. d) Eine Wasserheizung und Wasserkühlung.

25th shows a schematic representation of a perspective view of a receptacle 55 Temperature control 23 from obliquely below, with the magnets 20th to connect to the console 5 can be recognized. The recording 55 has a cavity 56 for the temperature control device 23 as well as openings 57 for fasteners on over which the recording 55 on the base plate 9 can be attached. The temperature control device 23 can be designed differently. The following are conceivable and applicable:

1. a) A small aluminum plate with an attached resistance heater that is passively cooled by the ambient temperature.
2. b) An aluminum plate with an attached resistance heater, which is cooled by active air cooling, for example with an air stream that is directed past the outside of the aluminum plate and, for the sake of simplicity, is taken from the air that is normally already cooled in the laboratory.
3. c) A Termo electric module (Peltier element) that actively heats and cools.
4. d) Water heating and water cooling.

In 26th is again the in 3 shown cassette 1 shown. The second positioning hooks are clearly visible 61 to recognize with which the cassette 1 form and force fit on the console 5 can be attached.

It should be noted that regardless of the specific design of the cassette 1 the overall concept of the invention presented here includes three components within the scope of ease of use, namely the first plate 10 (also called the base plate), the cassette bottom 2 and the cassette lid 3 who “arrange themselves”. This is, firstly, precise positioning of the removable component, ie the cassette 1 on a defined number of individual base plates 9 and, secondly, the preferably magnetic closure of the cassette bottom 2 and cassette lid 3 to a cassette 1 is to be understood. Looking at these three components, individual base plates 9 , Cassette bottom 2 and cassette lid 3 from bottom to top, it becomes clear that one or more permanent magnet (s) in the three components 20th , 21st are embedded, which are mounted directly on top of each other for optimal use of the magnetic forces. The cassette bottom 2 is in two places with permanent magnets 20th , 21st fitted. To form the cassette 1 become the magnets 20th in the three longitudinal struts 24 both at the cassette bottom 2 as well as in the cassette lid 3 appropriate. The magnets 21st for locking the cassette bottom 2 or the whole cassette 1 are next to the ram 51 behind the overflow channel 11 let in. The complementary magnets 21st are in the same place, i.e. next to the ram 51 in the base plates 9 let in. The user must therefore have a cassette 1 only in the "relative proximity" of the intended area of the console 5 move in which the cassette 1 should be used, the multiple base plates 9 includes. For example, relative proximity is a distance of 10 mm to approx. 20 mm from the magnets 20th to understand the base plates.

In 32 an embodiment of a method according to the invention is shown using a block diagram.

In a first step 65 the slides carrying the biological material 8th one holding device each 7th hung up before or after the holding devices 7th in the recording areas 6th of the cassette bottom 2 have been inserted. In a second step 66 become the cassette lid 2 and the cassette bottom 3 merged to thus the cassette 1 to close. Then in a third step 67 the cassette 1 with the console 5 connected. In a fourth step 68 the liquid or liquids are in the holding devices 7th brought in. If necessary, the samples are then processed further, for example the slides are lifted 8th and suctioning off the liquid from the reagent gap after a defined exposure time.

It should be noted that between each step 65 to 68 further steps can be taken, for example heating the slide. Furthermore, the steps 65 to 68 do not necessarily take place in the specified order.

With regard to further advantageous embodiments of the device according to the invention, reference is made to the general part of the description and to the appended claims in order to avoid repetition.

Finally, it should be expressly pointed out that the above-described exemplary embodiments of the device according to the invention only serve to explain the teaching claimed, but do not restrict them to the exemplary embodiments.

List of reference symbols

1

cassette

2

Cassette bottom

3

Cassette cover

4th

Cover disk

5

console

6th

Recording area

7th

Holding device

8th

Microscope slide

9

first record

10

second plate

11

Overflow channel

12

Electric motor

13

Deflection device

14th

Suction device

15th

Aspirator

16

Fluid connection

17th

Impressions

18th

Electromagnet

19th

Control means

20th

magnet

21st

magnet

22nd

Side wing

23

Temperature control

24

Longitudinal strut

25th

Spacers

26th

Side wall

27

Support shoulder

28

Back wall

29

Support point

30th

Tear-off edge

31

bulge

32

indentation

33

Slant

34

Pressure point

35

deepening

36

indentation

37

Lid strut

38

engine

39

bracket

40

Abutment

41

lever

42

anchor

43

feather

44

cover

45

casing

46

head Start

47

Passage

48

admission

49

gap

50

Step lip

51

Plunger

52

Positioning aid

53

Edge

54

Step seesaw

55

admission

56

cavity

57

openings

58

Inner wall

59

Slant

60

first positioning hook

61

second positioning hook

62

Suction opening

63

Connection opening

64

Filling passage

65

first step

66

second step

67

Third step

68

fourth step

Claims (19)

Device for wetting biological material with at least one liquid, comprising a cassette (1) with a cassette bottom (2) and a cassette lid (3), the cassette (1) having at least two receiving areas (6) for one holding device (7) each , wherein the holding device (7) serves to receive a slide (8) which carries the biological material, wherein the biological material arranged on the slide (8) can be wetted with the liquid within the closed cassette (1) and wherein the slide ( 8) within the closed cassette (1) can at least partially be lifted off the holding device (7) in order to allow liquid located between the specimen slide (8) and the holding device (7) to drain off, characterized in that on of the holding device (7) side wings (22) are formed and that indentations (32) are formed on the receiving area (6) on which the side wings (22) a fly up. Device according to Claim 1 , characterized in that the holding devices (7) can be removed from the cassette (1). Device according to Claim 1 or 2 , characterized in that at least part of the receiving area (6) is formed by a passage in the cassette base (2). Device according to one of the Claims 1 to 3 , characterized in that a console (5) is arranged, wherein the cassette base (2) can be placed on and removed from the console (5). Device according to Claim 4 , characterized in that the console (5) has a deflection device (13) so that each object carrier (8) can be lifted, preferably individually, within the closed cassette (1). Device according to Claim 4 or 5 , characterized in that a temperature control device (23) is arranged on the cassette (1) and / or on the console (5) for each receiving area (6). Device according to one of the Claims 1 to 6th , characterized in that the cassette lid (3) and the cassette bottom (2) can be connected to one another via magnetic forces, in particular via at least one permanent magnet (21). Device according to one of the Claims 1 to 7th , characterized in that the cassette lid (3) and the cassette base (2) can be connected to one another in a form-fitting and / or force-fitting manner, in particular via a clamping and / or latching mechanism (60), for example via a bayonet lock. Device according to one of the Claims 4 to 8th , characterized in that the cassette (1) and the console (5) can be connected to one another via magnetic forces, in particular via at least one permanent magnet (20). Device according to one of the Claims 4 to 9 , characterized in that the cassette (1) and the bracket (5) can be connected to one another in a form-fitting and / or force-fitting manner, in particular via a clamping and / or latching mechanism (61), for example via a bayonet lock. Device according to one of the Claims 1 to 10 , characterized in that a suction device (14) is designed for sucking off the liquid from the holding devices (7). Device according to Claim 11 , characterized in that the suction device (14) has suction means (15) which are arranged on the cassette cover (3) or on the cassette base (2) in order to suck off liquid from the holding devices (7). Device according to Claim 12 , characterized in that the suction means (15) are connected to a vacuum device, for example a pump, via a preferably common fluid connection (16). Device according to Claim 13 , characterized in that the fluid connection (16) is at least partially designed as a hose connection. Device according to Claim 13 or 14th , characterized in that pressure means (17) are provided in order to interrupt the fluid connection (16) of a suction means (15) with the negative pressure device. Device according to Claim 15 , characterized in that the push-off means (17) is formed by a pretensioned push-off element (41). Device according to Claim 16 , characterized in that the push-off means (17) can be moved counter to its preload by a release device (18), for example an electromagnet or an electric motor, in order to release the fluid connection (16). Device according to one of the Claims 1 to 17th , characterized in that the depth of the indentations (32) is greater than the thickness of the side wings (22). Method for wetting biological material with at least one liquid, using a device for wetting biological material with at least one liquid, comprising a cassette (1) with a cassette base (2) and a cassette cover (3), the cassette (1) has at least two receiving areas (6) each for a holding device (7), the holding device (7) serving to receive a slide (8) that carries the biological material, the biological material arranged on the slide (8) within the closed Cassette (1) can be wetted with the liquid and wherein the specimen slide (8) within the closed cassette (1) can at least partially be lifted off from the holding device (7) in order to prevent liquid that is between the specimen slide (8) and the Holding device (7) located to enable, or using a device according to one of the Claims 1 to 18th , with the following process steps: - inserting slides (8) carrying the biological material onto a respective holding device (7) before or after the holding devices (7) have been inserted into the receiving areas (6) of the cassette base (2), - merging the Cassette lid (3) and the cassette base (2) for closing the cassette (1), - connecting the cassette (1) to a console (5), and - introducing the liquid and / or the liquids into the holding devices (7).

Images