DE102016210850A1 - Fluid supply interface with backwash device, use of such a fluid supply interface for the management of cell culture containers and cell culture management system - Google Patents

Abstract

A fluid supply interface (10) comprises: - at least one supply coupling formation (20, 22, 24, 26, 28) for coupling each supply fluid line (34, 36, 38, 40, 42) to the fluid supply interface (10), - at least one disposal coupling formation (82, 84) for coupling one disposal fluid line (86, 88) to the fluid supply interface (10), - at least one consumer coupling formation (104) for coupling one consumer fluid line (108) to the fluid supply interface (10), - one fluid channel (16) comprising at least one supply coupling formation (20, 22, 24, 26, 28) interconnecting at least one disposal coupling formation (82, 84) and the at least one consumer coupling formation (104), - at least one supply valve (64, 66, 68, 70 , 72), through which depending on its position, a supply coupling formation (20, 22, 24, 26, 28) can be flowed through by fluid or for a Du rchströmung is locked, - at least one disposal valve (94, 96), through which depending on its position a Entsorgungskopplungsformation (82, 84) is flowed through by fluid or is blocked for flow, according to the invention the fluid supply interface is coupled to a backwash device (110), by which one and the same fluid in the fluid channel (16) can be driven to flow in opposite directions.

Description

• – wenigstens eine Versorgungskopplungsformation zur Ankopplung je einer Versorgungsfluidleitung an die Fluidversorgungsschnittstelle,
• – wenigstens eine Entsorgungskopplungsformation zur Ankopplung je einer von der Versorgungsfluidleitung verschiedenen Entsorgungsfluidleitung an die Fluidversorgungsschnittstelle,
• – wenigstens eine Verbraucherkopplungsformation zur Ankopplung je einer von der Versorgungsfluidleitung und der Entsorgungsfluidleitung verschiedenen Verbraucherfluidleitung an die Fluidversorgungsschnittstelle,
• – einen Fluidkanal, welcher die wenigstens eine Versorgungskopplungsformation, die wenigstens eine Entsorgungskopplungsformation und die wenigstens eine Verbraucherkopplungsformation miteinander verbindet,
• – wenigstens ein Versorgungsventil, durch welches abhängig von seiner Stellung eine Versorgungskopplungsformation von Fluid durchströmbar ist oder für eine Durchströmung gesperrt ist, und
• – wenigstens ein vom Versorgungsventil verschiedenes Entsorgungsventil, durch welches abhängig von seiner Stellung eine Entsorgungskopplungsformation von Fluid durchströmbar ist oder für eine Durchströmung gesperrt ist.

The present invention relates to a fluid supply interface for a cell culture system, the fluid supply interface comprising:

• At least one supply coupling formation for coupling each supply fluid line to the fluid supply interface,
• At least one disposal coupling formation for coupling each of a disposal fluid line different from the supply fluid line to the fluid supply interface,
• At least one consumer coupling formation for coupling each of a consumer fluid line different from the supply fluid line and the disposal fluid line to the fluid supply interface,
• A fluid channel which connects the at least one supply coupling formation, the at least one disposal coupling formation and the at least one consumer coupling formation,
• - At least one supply valve through which depending on its position, a supply coupling formation of fluid is flowed through or is blocked for a flow, and
• - At least one disposal valve different from the supply valve, through which depending on its position a disposal coupling formation of fluid can flow or is blocked for a flow.

Such a fluid supply interface is known from DE 10 2013 201 069 A1 known. It serves to manage a plurality of cell culture containers, ie to provide them with nutrient media, to dispose of used media, optionally to clean the cell culture containers and to harvest cells of a finished cultivated cell culture at the end of a cultivation cycle. The fluid supply interface allows the successive coupling of different cell culture containers to the consumer coupling formation so that a plurality of cell culture containers can be managed with a single fluid supply interface.

With the fluid supply interface mentioned above cell cultures can be managed extremely economically. Decisive for the success, however, is that there is no cross-contamination between the cell culture containers managed by a common fluid supply interface. Serve for this purpose, the said valves, which allow the separation of a fluid line from the fluid channel.

It is therefore an object of the present invention to better protect the above-mentioned fluid supply interface and a cell culture management system using it against the risk of cross-contamination.

This object is achieved by a generic fluid supply interface, which is coupled to a backwash device through which one and the same fluid in the fluid channel to the flow in opposite directions can be driven.

Thus, for example, the fluid channel may be filled with cleaning fluid, and then the cleaning fluid column present in the fluid channel may be moved in opposite directions to provide mechanical cleaning action through fluid movement in addition to the chemical cleaning action due to the fluid composition, avoiding any flow shadow areas into which a cleaning fluid would flow only insufficiently in flow in only one direction of movement.

However, the cleaning fluid is only a particularly preferred example. The use of the backwash device should not be limited thereto. Thus, it may also be advantageous to use the backwashing device in conjunction with other fluids, for example to effect the most complete possible wetting of the fluid channel and all possibly protruding in this valve body before this or another fluid in a cell culture container on or is discharged from this ,

To achieve the mentioned backwashing effect, it is advantageous if the backwashing device comprises a direction reversible pump. This may be a reversible, continuously operating pump, or it may be - and this is preferred due to the achievable precise fluid movement - a piston-cylinder arrangement. The piston can, for example, be driven by a rotating eccentric drive for reciprocating movement in the cylinder, wherein it moves the liquid column present in the fluid channel depending on the respectively prevailing piston movement in one or in the opposite other direction.

In order to be able to operatively separate the backwash device from the fluid channel if desired, provision may be made for a backwash fluid line which connects the direction-reversible pump to the fluid channel to be provided upstream of the supply valve in the supply flow direction from the supply coupling formation to the consumer coupling formation.

The at least one supply valve is to avoid unwanted access to Fluid channel is preferably biased into a blocking position preventing the flow through the supply coupling formation associated with it. In order to form the supply valve with as few components and hold in a defined operating position, the supply valve is preferably biased by magnetic force in the blocking position. This also serves the hygiene of the device, because with less component count also decreases the risk of contamination in the fluid supply interface.

In order to ensure that fluid can always be conveyed in the direction of supply to the fluid channel through the supply coupling formation, the at least one supply valve is preferably designed such that, due to sufficiently high pressure difference on both sides of the supply valve against the, preferably magnetic, biasing force for flow in the direction of the Supply coupling formation opens to the fluid channel. This can be realized with a magnetic preload in the blocking position structurally particularly simple. Likewise, the disposal valve can be biased in a closed flow preventing its closed position.

Regardless of whether the disposal valve is biased by a magnetic or mechanical biasing device, by an elastomeric or metallic spring means in the closed position, it is for targeted non-contact and therefore high-purity operation of the disposal valve advantageous if it by changing an external magnetic field between its operating positions: Closed position and flow position, can be switched. The externally applied to the disposal valve magnetic field and the biasing device of the disposal valve must therefore be coordinated so that the force exerted by the external magnetic field on the at least one disposal valve force can overcome the biasing force in the closed position. Also for this vote, the use of helical compression springs as a mechanical biasing device of the disposal valve is advantageous.

The disposal valve may comprise a displaceable actuator, which is displaced by the external magnetic field and in turn moves in its displacement a valve body of the disposal valve from the closed position to the flow position. For reasons of the smallest possible number of necessary for the formation of the disposal valve components, it is advantageous if the disposal valve has a valve body with or made of ferromagnetic and / or permanent magnetized material. Then, the external magnetic field can act directly on the valve body and move it between its operating positions.

The same applies to the supply valve. Regardless of the design of the disposal valve, this is also preferably by changing an external magnetic field between its operating positions: blocking position and passage position, switchable to set from the outside targeted a desired operating position can. The supply valve therefore also preferably has a valve body with or made of ferromagnetic or / and permanently magnetized material for the reasons stated above.

Switchability of the at least one disposal valve and / or the at least one supply valve by changing an external magnetic field, the respective valve, for example, be switched independently of a switching the valves switching device manually in a desired operating position.

The change of an external magnetic field can be effected by changing a Bestromungszustands of an electromagnet located sufficiently close to the respective valve or by approaching and removing a permanent magnet to the valve. The use of an approachable and removable permanent magnet is preferred over the use of an electromagnet because the electromagnet can generate a heat source when energized, which is undesirable in the case of using thermally sensitive fluids or thermally sensitive cells.

The named "two sides" of a valve are one side in the flow direction in front of a valve body and one side in the flow direction behind the valve body.

Since the presently presented fluid supply interface is preferably used for the management of cell culture containers of a cell culture system, a corresponding, at least partially complementary consumer negative feedback formation of a cell culture container is temporarily coupled to the consumer coupling formation. The consumer negative feedback formation is connected to the cell culture vessel via a consumer fluid line. The fluid channel interconnecting the fluid supply interface coupling formations referred to in the present application may thus serve as a reference for describing flows through the supply interface. If the fluid channel has a spatially excessive extent in order to serve as a suitable reference location in an individual case, the at least one consumer coupling formation can be used as Serve reference. If a plurality of consumer coupling formations are provided, for example because more than one consumer negative feedback formation is formed on each cell culture container, one of the plurality of consumer coupling formations can be arbitrarily selected, it being merely necessary to ensure that the same consumer coupling information of the fluid supply point is always used within a judgment situation.

Thus, while the at least one consumer coupling formation is configured for the purpose of merely temporary coupling of a consumer negative feedback formation, the at least one supply coupling formation and the at least one disposal coupling formation are preferably designed for permanent coupling of a supply or disposal fluid line. The training for "permanent" coupling is not to be understood that the respective fluid line can only be separated destructive of their coupling formation. "Permanent" in the sense of the present application, the coupling of a fluid line to the coupling formation already when it can be solved for repair and maintenance purposes of the coupling formation, but remains permanently connected to the supply point during normal operation of the supply interface, while the Consumer coupling formation is repeatedly coupled with Verbrauchergegenkopplungsformationen different cell culture container.

The supply or / and the Entsorgungskopplungsformation can be formed, for example, by pipe sockets on which a hose or a pipe end can be pushed and secured by a suitable securing means, such as hose clamps and the like, against peeling. However, said coupling formations can also be formed by hose or pipe coupling components, one of which is provided on the supply or the disposal coupling formation and the other on the associated fluid line, so that they can be connected together to form a continuous fluid line.

The fluid channel can be formed in a simple but flexible construction of the fluid supply interface by a hose, in which the lines coupled to the at least one supply and the at least one disposal coupling formation open.

Since the fluid supply interface for coupling to different cell culture containers preferably by a handling device, such as a multi-axis robot, is movable to different coupling locations, it is preferred if the fluid channel and thus the fluid supply interface is formed as stiff as possible. The fluid supply interface therefore preferably has a substantially rigid housing in which the fluid channel is formed. The consumer coupling formation may preferably be integrally formed on the housing.

The at least one supply coupling formation and / or the at least one disposal coupling formation can be formed integrally with the housing or mounted as formation assemblies in corresponding receptacles on the housing. The latter is preferred for reasons of simpler manufacture of the fluid supply interface. At least one part, preferably all supply and / or disposal valves, are preferably located in the housing.

The housing may be designed in several parts to realize, for example, complex housing designs. Preferably, however, the housing is integrally formed.

Although the fluid channel may in principle have any profile in the fluid supply interface and in particular in its housing, a fluid channel with straight course is advantageous for the fluid supply interface according to the invention, at least with a rectilinear profile between the supply coupling formation furthest from the at least one consumer coupling formation and the furthest from the same at least one consumer coupling formation provided Entsorgungskopplungsformation.

Therefore, the fluid channel is preferably formed either as a rectilinear blind hole in the housing of the fluid supply interface, which is preferably closed at its opening side by a plug or the like or closed by a valve, or is formed by a through hole passing through the housing, on one or both Closed longitudinal ends in the housing or can be closed by a valve. The blind hole as well as the through hole can be made simple in manufacturing technology and therefore advantageous as holes.

The at least one supply coupling formation and the at least one disposal coupling formation are preferably provided on different sides of the at least one consumer coupling formation in order to prevent a flow-mechanical conclusion between a supply coupling formation and a disposal coupling formation in which the gas flowing between these coupling formations Fluid does not reach the consumer coupling formation. The at least one supply coupling formation and the at least one disposal coupling formation are therefore preferably arranged in the fluid supply interface such that a fluid flow existing or arising between them in the fluid channel always flows past the consumer coupling formation.

In principle, the at least one disposal valve - as well as the at least one supply valve - have a magnetic biasing device.

To facilitate the sizing of the valves, their biasing means and any external magnetic field sources, it is preferred if the at least one disposal valve and the at least one supply valve have the same valve bodies. The valve bodies are preferably spherical, that is spherical, so that their relative orientation to their surroundings in the fluid supply interface does not matter.

In order to avoid that a valve body, if it is lifted from its valve seat of the respective valve is then carried away with the then allowed fluid flow or / and gravitationally device from the sphere of influence of its biasing device, so that it is no longer returned to the locked position, it is advantageous if at the fluid supply interface, a holding formation is formed, which holds a valve body of the supply valve in its passage position with lifted from a valve seat valve body in the influence of the, preferably magnetic, biasing force. Preferably, the holding formation is formed in the fluid channel, so that the valve body of the supply valve preferably lifts off from its valve seat into the fluid channel.

Preferably, the retaining formation is formed as a wall formation, in particular a concave, approximately negative-dome-shaped, wall formation, of the fluid channel, so that no separate components are required for this purpose. The wall formation can be produced, for example, by machining a section of the housing forming the fluid channel in the region of the respectively affected valve. The holding formation may extend in sections orthogonal to the direction of travel of the fluid channel in the region of the respective valve into the material defining the fluid channel. For machining a wall formation in the fluid channel as a holding formation for the valve body of a supply valve, it is possible to use, for example, a face cutter and / or roll milling cutter, in particular ball milling cutters, shaped according to the desired wall formation.

The valve body of the at least one supply valve preferably already extends into the fluid channel in the blocking position of the at least one supply valve, but preferably not more than half its extent in the direction orthogonal to the fluid channel course. Thus, it can be achieved that the valve body, although flushed by a flow in the valve channel, but is not carried away by this.

It is likewise preferred if the valve body of a supply valve covers less than its greatest extent in the lifting direction, more preferably less than two thirds of its extent in the lifting direction, on its lifting position between its blocking position and its position lifted off at maximum from the valve seat. Particularly preferably, between its blocking position, in which it rests against a valve seat, and its maximum lifted position, the valve body does not rest more than half of its greatest extent in the lifting direction. In the preferred use of a spherical valve body, the maximum dimension of the valve body in any direction is always the diameter thereof.

In case of doubt, the fluid channel should continue to be considered at the mouth of fluid lines that open into the fluid channel, according to its training to one or both sides of the mouth over the mouth point away to the Einragtiefe usually located in the blocking position in the opening fluid line valve body in to determine the fluid channel.

Further, undesirable tearing of a valve body from its valve can be prevented by making the flow cross-section of the fluid channel smaller than a cross-sectional dimension of the valve body orthogonal to the trajectory of the fluid channel in the adjoining section so that penetration of the valve body into the fluid channel is prevented.

In order to limit the stroke of the valve body from the closing or blocking position, ie away from the valve seat, without additional components, it is advantageous if the fluid channel with the lifting direction of the valve body at least one supply valve and / or at least one disposal valve an angle, preferably includes a right angle. In this case, a wall portion of the fluid channel, in particular a previously described designed as a holding formation wall portion of the fluid channel, together with the valve seat to limit the movement stroke of the valve body between its operating positions are used.

If in the present application of an operating position of the valve body: blocking position or passage position, the speech, this is synonymous with the operating position of the supply valve itself. The same applies to the operating positions of the valve body of the disposal valve and the operating positions of the disposal valve.

• – durch den Fluidkanal miteinander verbunden sind und
• – je ein Versorgungsventil aufweisen, das in eine die Durchströmung verhindernde Sperrstellung vorbelastet ist, vorzugsweise durch Magnetkraft.

Usually, the management of cell culture containers requires the targeted delivery of more than one fluid to a respective coupled cell culture container and thus to the fluid supply interface, which is why it is provided that the fluid supply interface has a plurality of supply coupling formations, which:

• - Are interconnected by the fluid channel and
• - Have each a supply valve which is biased into a flow-preventing blocking position, preferably by magnetic force.

For the reasons already mentioned above, it is advantageous if a plurality, preferably all, supply valves open by sufficiently high pressure difference between the two sides of the respective supply valve against the, preferably magnetic, biasing force for flow in the direction of the respective supply coupling formation to the fluid channel.

The fluid supply interface preferably has a switching device with at least one magnet, also referred to below as a "switching magnet", through which an external magnetic field generated by the at least one magnet can be changed at a supply switching location to a supply valve provided at the supply switching location in a targeted manner between its operating positions to be able to switch. By the switching device, at least a portion of the provided supply valves can be switched between their respective operating positions without contact, which minimizes or even precludes the risk of deterioration of the quality of the passed through the supply valves fluids through the switching device.

It is true that the present invention also includes a fluid supply interface having a plurality of supply coupling formations interconnected by the fluid channel and each having a supply valve configured as described above without any switching device. However, this is not preferred since it is advantageous if a part of the supply valves can be selectively switched between their operating positions independently of the respective fluid pressure and fluid pressure difference prevailing at the valve location.

In order to reduce the complexity of the supply interface and the components necessary for its construction, it is preferably provided that not all existing supply valves can be switched by the switching device. It may be sufficient to provide only those supply valves for supply fluid lines switchable by the switching device which conducts particularly sensitive fluids. Therefore, the number of supply switching locations may be less than the number of supply valves. It is all about the simultaneous switchability of supply valves. Thus, it may theoretically be conceivable that a magnet is displaceable in such a way that it interacts with a first supply switching location for switching a first supply valve at a first time and with a second supply switching location different therefrom for switching a second supply time Supply valve cooperates. Consequently, the first and second supply valves are not switchable simultaneously. This case also falls under the above definition that the number of supply switching points is less than the number of supply valves.

A supply switching location is associated with a supply valve and therefore typically is stationary with respect to the fluid channel and / or a housing of the fluid supply interface.

Advantageously, the at least one disposal valve can also be switched between its operating positions by the switching device independently of the fluid pressure prevailing at the at least one disposal valve.

Furthermore, it may be necessary to manage a plurality of cell culture containers, to divert different fluids from the fluid channel or from the respectively coupled cell culture container. For this purpose, a plurality of Entsorgungskopplungsformationen be provided, which are interconnected by the fluid channel and each have a disposal valve, wherein by the switching device at a disposal switching a generated by the at least one magnet external magnetic field is changed to a disposal at the disposal provided Disposal valve to be able to switch between its operating positions. The number of disposal switching locations is the same as or less than the number of disposal valves. It applies here to the at least one disposal valve and its interaction with the switching device, the above to the plurality of supply valves and their interaction with the switching device said accordingly.

Preferably, a plurality of disposal valves constructively constructed as the supply valves to the supply coupling formations and has only one of these deviating function, namely a serving at least predominantly the discharge of fluids from the fluid channel function. In constructive with the supply valves identical disposal valves, the valve body preferably lifts off to the fluid channel out to switch the disposal valve in the flow position. Therefore, for such a disposal valve the above to the at least one supply valve What mutatis mutandis mutatis mutandis accordingly, and in particular in connection with the holding formation to secure the whereabouts of the valve body to the respective valve. For one or more further disposal valves, therefore, a retaining formation designed as described above is preferably provided at the fluid supply interface.

Likewise, preferably, the at least one disposal valve as well as the at least one supply valve on a magnetic biasing means to bias the valve body in the closed position.

Preferably, the distance between a disposal valve and a supply valve in the longitudinal direction of the fluid channel is greater than the distance in the same direction between two disposal valves and / or between two supply valves. As a result, the consumer coupling formation can be arranged between a disposal and a supply valve and be influenced by an external magnetic field, without the risk that the magnetic field acting at the location of the consumer coupling formation, that is to say at a consumer switching location, may also be undesirably applied to a supply or a supply Disposal valve acts and unintentionally causes a changeover of the operating position.

According to the above, it can be provided in a further development of the fluid supply interface according to the invention that by the switching device at a consumer switching location, which is located in the consumer coupling formation and on which a consumer fluid line with a consumer valve is temporarily arranged, generated by a magnet outer Magnetic field is changeable in order to switch the provided at the consumer switching point consumer valve between its operating positions: locking position and flow position. Preferably, in the case that a plurality of consumer coupling formations are provided, as many consumer switching locations are provided as consumer coupling formations to ensure that each consumer valve temporarily arranged on a consumer coupling formation is switchable independently of the other consumer valve.

The present invention further relates to a use of a fluid supply interface as described above and further developed for the management of cell culture containers, to each of which at least one consumer negative feedback formation for temporary coupling with the at least one consumer coupling formation is provided.

Furthermore, the present invention relates to a cell culture management system with a fluid reservoir, with a disposal sink and with a fluid supply interface, as described above and further developed. In this case, the fluid reservoir is coupled via a supply fluid line to the supply coupling formation, the disposal sink is coupled via a disposal fluid line with the disposal coupling formation and a cell culture container is detachably coupled to the at least one consumer coupling formation.

A cell culture container or a plurality of cell culture containers need not be part of the cell culture management system, but is preferably part of the same.

A fluid reservoir may comprise a container with a fluid reservoir or, for example in the case of tap water, may comprise a fluid-carrying supply line.

Likewise, the disposal sink may comprise a container in which discarded fluid is collected from a cell culture container. This is especially the case when the fluid discharged from the cell culture container is the desired result of the cell culture. Alternatively, the disposal sink can be a piping system, such as a wastewater or waste disposal system of a local authority in which the cell culture management plant is operated.

In order to also effect a fluid movement with the backwashing device on the valve to be flushed or blocked, it is advantageous for the backwashing fluid line in the supply or supply flow direction from the supply coupling formation to the consumer coupling formation to flow upstream of the supply valve into the supply fluid line.

Furthermore, it may be desired that the backwashing device, when performing a backwashing operation, has no effect on the fluid supply coupled to the same supply coupling formation as the backflushing device and in which generally high purity fluid is provided. Therefore, according to A further preferred development of the present invention provides that in the feed flow direction from the supply coupling formation to the consumer coupling formation upstream of the mouth of the backwash fluid line into the supply fluid line, a shut-off valve is provided, through which a section of the supply fluid line located upstream of the mouth can be separated from the backwash fluid line.

It should be noted that the indication of a flow direction from one point to another point in a fluid line not only relates to the flow state between said points, but indicates a flow direction which applies to each point of a fluid line fluidically connected to said points in fluid communication.

In order to be able to cultivate cell cultures in cell culture containers as comprehensively as possible by the cell culture management plant presented here, the cell culture management plant has a cleaning fluid reservoir as the previously mentioned fluid reservoir and additionally has at least one nutrient media reservoir as a further fluid reservoir. Furthermore, the cell culture management system preferably additionally has a harvest sink as a further sink, into which fluid is conveyed out of a cell culture container coupled to the fluid supply interface, in order to be able to harvest finished cultivated cell cultures and process them as intended.

The cleaning fluid supply is then coupled to a first supply coupling formation via a first supply fluid line, wherein the first supply fluid line is preferably the supply fluid line into which the backwash fluid line opens, as described above.

Likewise, the at least one nutrient media supply is coupled to a second supply coupling formation via a second supply fluid line.

The cell culture management system may further include one or more functional fluid supplies, such as for providing a rinse fluid to purify adherent cells in the cell culture container of nutrient medium, and / or for providing a release fluid to dislodge adherent cells in the cell culture container from their substrate surfaces. These functional fluid supplies may be coupled by third and even further supply fluid conduits with third and yet further supply coupling formations.

The disposal sink is coupled via a first disposal fluid line to a first disposal coupling formation. The disposal sink serves as a so-called "waste" sink of the final disposal of a coupled to the supply interface cell culture container and / or discharged from the supply interface itself fluid.

The harvest sink is preferably coupled to a second disposal coupling formation via a second disposal fluid line.

Although the harvested coupling formation is preferably designed as a disposal formation for discharging fluid from a cell culture container or from the fluid supply interface, it is structurally designed like a supply coupling formation. Preferably, each of the existing disposal valves can be switched by the switching device between its operating positions in order to specifically discharge fluid through exactly the desired of several disposal fluid lines.

Of the supply valves, preferably only that of the first supply fluid line coupled to the cleaning fluid supply can be switched by the switching device. For the second and each further supply fluid line, it may be sufficient if they are to be opened by overpressure in the respective supply fluid line with respect to a fluid pressure prevailing in the fluid channel.

For example, a third supply coupling formation may be provided which is coupled via a third supply fluid line to a wash fluid supply for purifying cultured cells of nutrient medium.

Likewise, a fourth supply coupling formation may be provided, which is coupled to a supply of trypsin-EDTA via a fourth supply fluid line to effect detachment of adherent cells from an inner wall of a cell culture container coupled to the fluid supply interface.

In order to facilitate the coupling of a cell culture container, the at least one supply coupling formation and the at least one disposal coupling formation on the one hand and the consumer coupling formation with respect to the fluid channel lie on different sides of the fluid supply interface.

The cell culture management system may comprise at least one pump in order to generate a positive pressure in a fluid line (usually in a supply fluid line) or a negative pressure (usually in a disposal fluid line).

Preferably, only one fluid line is connected to each pump in order to avoid cross-contamination here as well. However, the provision of pumps is not absolutely necessary. On the one hand, a fluid reservoir may be under positive pressure or a drain may be under negative pressure with respect to the atmospheric pressure. By means of suitable control valves, the overpressure prevailing in a respective reservoir or the negative pressure prevailing in a respective depression can then be coupled into the connected fluid line. On the other hand, at least one fluid reservoir opposite the fluid supply interface and the cell culture container coupled thereto can be arranged increased in the opposite direction to the direction of gravity, so that a hydrostatic pressure difference between the fluid reservoir and fluid channel is established due to the height difference between the fluid reservoir and the fluid channel. Again, the generated hydrostatic overpressure in a supply fluid line to a fluid supply can be exercised by suitable control valves on the respective connected supply valve or not exercised.

The present invention will be explained in more detail with reference to the accompanying drawings. It shows:

1 2 shows a schematic and partially roughly schematic sectional view through a fluid supply interface according to the invention of a likewise cell culture management system according to the invention,

2 the view of 1 with an open supply valve and also open consumer valve,

3 the view of 2 in which, instead of a faulty non-opened consumer valve, a disposal valve is opened by overpressure in the fluid channel,

4 the view of 3 in which the disposal valve is connected by the magnet of a switching device in a flow-through position and

5 the view of 1 to 4 at an open another supply valve and the open disposal valve, which are each adjusted by a switching device in the passage or flow position, for cleaning operation using a backwash.

In 1 is a fluid interface according to the invention generally with 10 designated. It is part of a cell culture management system 12 ,

In the example shown, the fluid supply interface 10 a preferably one-piece housing 14 on, that of a fluid channel 16 , which preferably extends along a rectilinear fluid channel axis FK, is penetrated in its longitudinal direction. The fluid channel preferably produced as a through-bore 16 is at its two longitudinal ends by a plug 18 closed at the end.

In the example shown, the fluid supply interface 10 five supply coupling information, hereinafter referred to as first to fifth supply coupling formation 20 . 22 . 24 . 26 and 28 are named. The supply coupling information 20 to 28 are preferably identical, which is why representative of all supply coupling information below 20 to 28 only the first supply coupling formation 20 will be described.

The supply coupling information 20 to 28 For example, a pipe socket 30 have on which a hose 32 a supply fluid line 34 deferred and, for example, by a hose clamp 35 against unwanted removal from the pipe socket 30 can be secured. The pipe socket 30 may also have a peeling aggravating the peeling.

Each supply coupling formation 20 to 28 is a supply fluid line 34 to 42 each associated with a hose and can be coupled via this in the manner described above with the respective supply coupling formation permanently within the meaning of the present application for fluid transfer. The supply fluid lines are subsequently named with the same ordinal number as the supply coupling formation to which it is connected, so that in the present example a first to fifth supply fluid line 34 . 36 . 38 . 40 and 42 gives.

In addition, in the present example, each supply fluid line 34 to 42 one supply pump each 44 to 52 associated, which can increase the fluid pressure in their respective associated supply fluid line to the flowing through the respective supply fluid line fluid in the fluid channel 16 to promote. If necessary, the supply pumps 44 to 52 subsequently named with the same ordinal number as the supply fluid lines assigned to them, so that there is a first to fifth supply pump 44 . 46 . 48 . 50 and 52 gives. The supply coupling information 20 to 28 are intended in the supply direction to the fluid channel 16 flows through with fluid.

Next is each supply fluid line 34 to 42 each with a fluid supply 54 to 62 connected, from which via the supply pumps 44 to 52 Fluids to the fluid channel 16 can be promoted.

For example, the first fluid supply 54 a cleaning fluid reservoir, in which water or a specifically matched to the particular cultivation case cleaning agent or air can be stored.

In the second fluid supply 56 For example, a nutrient medium with nutrients for the cells to be cultivated in the cell culture container 63 be saved.

In the third fluid supply 58 For example, a cleaning fluid, such as phosphate-buffered saline ("PBS"), can be stored, which allows cells of a cell culture to be purified from nutrient medium adhering thereto.

In the fourth fluid supply 60 For example, a medium for detaching adherent cells may be stored by its surface substrate to which it adheres. Such a medium is, for example, trypsin-EDTA.

The fifth fluid supply 62 can store a reserve nutrient medium to ensure an uninterrupted supply of cell cultures with nutrient medium. The allocation of fluid supplies 54 to 62 However, it may of course also be different from the above example.

The supply coupling formation 20 , which may be formed in one or more pieces, has at its the fluid channel 16 closer longitudinal end of a supply valve 64 on. So that every supply fluid line 34 to 42 by one supply valve depending on its operating position either blocked for a fluid flow or is permeable to flow, has the fluid supply interface 10 a first to a fifth supply valve 64 . 66 . 68 . 70 and 72 on.

By way of example, the first supply valve will be described below 64 for all other identically designed supply valves 66 to 72 described. The first supply valve 64 has a spherical valve body 74 which is made of ferromagnetic but not magnetized material. This spherical valve body 74 rests in the blocking position of the supply valve 64 on a soft-elastic valve seat 76 , such as silicone elastomer, on which the valve body 74 in its locking position with slight deformation of the valve seat 76 sealingly rests. The valve body 74 is through an annular permanent magnet 78 , which is flowed through in its central passage of fluid, magnetically in the in 1 biased locked position shown.

In the blocking position, the valve body protrudes 74 preferably with no more than half its diameter in the fluid channel 16 into it.

To the transition into its passage position, in which the first supply valve 64 a fluid flow from the first fluid reservoir 54 to the fluid channel 16 lets through, the valve body becomes 74 preferably in a direction to the axis FK of the fluid channel 16 orthogonal lifting direction A moves. It is not excluded that the valve body 74 in addition to a movement in Abheberichtung A performs a slight sideways movement orthogonal to the lifting direction A. However, this lateral movement is not important. Switching the operating position of the first supply valve 64 like all other supply valves 66 to 72 , always occurs when the valve body 74 in lifting direction A from the valve seat 76 Will get removed.

The fluid channel is preferred 16 between two adjacent supply valves or altogether between two adjacent valves having a diameter which is smaller than the diameter of the valve body 74 to prevent the valve body 74 through him or in the case of valves further downstream 76 to 72 from an upstream valve - allowed flow is swept away and no longer on the valve seat 76 can be moved back into its blocking position.

To hold the valve body 74 in its passage position near the bias magnet 78 can continue to support, a holding formation 80 be provided, which, for example, directly as a formation of the fluid channel 16 enclosing wall in the housing 14 be educated. For example, the holding formation 80 the bias magnet 78 in the lifting direction A opposite as a concave wall formation with respect to and within the cylindrical wall of the fluid channel 16 be educated. While the cylindrical wall of the fluid channel 16 only around the course axis FK of the fluid channel 16 Curved, the holding formation can 80 in addition to be curved about a both the fluid channel axis FK and the lifting direction A orthogonal axis of curvature. The holding formation 80 can then for the valve body 74 form a Schmiegefläche where it rests in its passage position.

In the present example, the fluid supply interface according to the invention 10 a first disposal coupling formation 82 and a second disposal coupling formation 84 on.

Although the disposal coupling formations 82 and 84 can be flowed through in opposite directions, they are the intended use of the fluid supply interface 10 predominantly or even exclusively in the disposal direction of the fluid channel 16 flows through it. The disposal coupling formations 82 and 84 are as well as the supply coupling formations 20 to 28 for coupling hoses of disposal fluid lines 86 and 88 educated.

The first disposal coupling formation 82 is about the first disposal fluid line 86 with a first disposal sink 90 connected in the form of a waste container. The disposal fluid line 86 thus forms a "waste" line. Any intermediate or final product of cell cultivation that is discarded will go through the first disposal fluid line 86 the waste container 90 fed. To effect the fluid flow in the direction of disposal, the waste container 90 be under relative negative pressure or between the disposal coupling formation 82 and the waste container 90 can in the disposal line 86 be provided a delivery pump.

The second disposal coupling formation 84 is via the second disposal fluid line 88 with a harvest container 92 coupled, in which finished cultured cell cultures from the rudimentary cell culture container 63 can be promoted. Again, the harvest container 92 be under a relative negative pressure, or there may be a feed pump in the second disposal fluid line 88 be arranged to effect a fluid flow in the disposal direction.

The first disposal fluid line 86 is through a first in the fluid supply interface 10 disposed disposal valve against a flow closable or flowed through. The second supply fluid line 88 is through a second disposal valve 96 closable or permeable.

The second disposal coupling formation 84 with the second disposal valve 96 in the present example is identical to the first to fifth supply coupling information 20 to 28 with their first to fifth supply valves 64 to 72 designed so that the description of the second disposal coupling formation 84 with the second disposal valve 96 to the previous description of the supply coupling information with the supply valves.

The first disposal valve 94 In contrast to all previously described disposal valves is constructed as a safety valve. It comprises a ferromagnetic, but preferably not permanently magnetized valve body 98 , which in his in 1 shown closed position on a valve seat formed of soft elastic, elastomeric material 100 sealingly rests. The valve body 98 is preferred with the valve bodies 74 the other valves 64 to 72 and 96 identical. However, the valve body is 98 not by magnetic force, but by a helical compression spring 102 in the in 1 biased shown closed position. By selecting a suitable spring steel or spring metal in general, by its dimensioning (spring cross section) and by the dimensioning of the biasing spring 102 itself, that can be used to adjust the valve body 98 necessary force can be set constructively very accurately in its flow position.

The helical compression spring 102 is preferred as a biasing spring, since it acts on the valve body 98 allowed with an approximately passing through the center of biasing force and at the same time can be flowed through by fluid. The helical compression spring 102 is arranged such that fluid in the first disposal fluid line 86 radially inside the turns of the helical compression spring 102 can flow past. Although the valve body could 98 of the first disposal valve 94 additionally or alternatively be biased by magnetic force in the closed position, but preferred is the exclusive bias with mechanical spring means.

For adjustment of the valve body 98 from the in 1 shown closed position in his from the valve seat 100 lifted flow position is the valve body 98 in an opening direction B movable, which is opposite to the lifting direction A of the other valve body in the example shown. Both the lifting direction A and the opening direction B are preferably collinear with the course direction of the respective fluid lines 34 to 42 . 86 and 88 in the area of their connection to the fluid supply interface 10 ,

Due to its formation of ferromagnetic material is the valve body 98 of the first disposal valve 94 basically by a change of an external magnetic field, ie by magnetic force, from the in 1 shown closed position switchable in its flow position. For this purpose, the solenoid and the biasing spring 102 be coordinated with each other, that of the switching magnet in the switching active state on the valve body 98 applied force the power of Hooke's bias spring 102 predominates.

In the present example, the supply coupling formations 20 to 28 and the disposal coupling information 82 and 84 in each case one block of coupling formations in which only functionally identical coupling formations, supply coupling formations on the one hand and Disposal coupling information on the other hand, are arranged. Between the only adjacent in the illustrated example coupling formations 28 and 84 different function, the distance along the fluid channel axis FK is greater than between two immediately adjacent in the same direction supply coupling information 20 to 28 on the one hand and disposal coupling information 82 and 84 on the other hand.

The pipe socket 30 the coupling formations 20 to 28 . 82 and 84 are preferably parallel to each other and are all on the same side with respect to the fluid channel 16 ,

In the area between the coupling formations 28 and 84 the greater distance between these is used for a consumer linkage formation 104 train. To facilitate the coupling of consumers, such as cell culture containers 63 it opens the consumer link formation 104 with respect to the fluid channel 16 on another, preferably on an opposite side as the coupling formations 20 to 28 . 82 and 84 ,

In the 1 to 5 is to the consumer linkage formation 104 a cell culture container 63 coupled shown. This has a consumer valve 106 on, which is identical to the supply valves 64 to 72 and to the second disposal valve 96 is trained. Unlike the supply valves 64 to 72 and to the disposal valve 96 is the consumer valve 106 but not stationary at the fluid supply point 10 but fixed to the cell culture container 63 arranged. A consumer fluid line 108 connects the consumer valve 106 with one in the 1 to 5 Not completely illustrated, but generally known in the art cell culture container body.

The fluid supply interface 10 is still with a backwash 110 coupled, which in the example shown with the first supply fluid line 34 is connected through the cleaning fluid in the fluid channel 16 can be promoted. The backwash device 110 , which will be discussed in more detail below, comprises in the example shown a piston-cylinder arrangement 112 with one in a cylinder 114 along the cylinder axis Z movable piston 116 , This is one to the first supply fluid line 34 connected volume 118 the backwash device 110 mutable.

The backwash device 110 is with the first supply fluid line 34 via a shut-off valve 120 connected, which is adjustable, between a position in which alone the backwash 110 with the first supply coupling formation 20 fluidly connected, and a position in which alone the first fluid reservoir 54 with the first supply coupling formation 20 fluidly connected.

The shut-off valve 120 may be biased in one of its two positions, preferably in that position in which the first fluid supply 54 fluidly communicating with the first supply coupling formation 20 is coupled.

The shut-off valve 120 can replace the multi-position multiway valve of 1 however, alternatively, it may be constituted by a simply constructed check valve which directs fluid flow only in the direction of the first fluid supply 54 to the first supply coupling formation 20 lets through and locks in the opposite direction. In this case, the alternative shut-off valve 120 ' upstream of the only possible flow direction of a then permanently established junction of a connecting line 122 in the first supply fluid line 34 arranged. This alternative is in 2 shown.

Through the shut-off valve 120 respectively. 120 ' may have a retroactive effect on the operation of the backwashing device 110 on the first fluid supply 54 or in total to one between the shut-off valve 120 respectively. 120 ' and the first fluid supply 54 located portion of the first supply fluid line 34 be prevented.

In 2 is the view of 1 at changed operating position of the third supply valve 68 and the consumer valve 106 shown.

The consumer valve 106 was through a solenoid 124 a switching device switched to its passage position. Also in the area of consumer linkage formation 104 is a substantially similar concave, optionally dome-shaped retaining formation 80 on the wall of the fluid channel 16 formed to the valve body of the consumer valve 106 , which, moreover, is preferably identical to the other valve bodies, in the vicinity of the biasing magnet biasing it into its locking position. The consumer valve 106 in the example shown is identical to the supply valves 64 to 72 and to the second disposal valve 96 so that its description to the above description of the first supply valve 64 is referenced.

While the consumer valve 106 in the present invention for supplying fluid into the cell culture container 63 always by the solenoid 124 the switching device in his in 2 shown flow position is switched for example, the valve body of the third consumer valve 68 alone by activating the pump 48 and as a result of the pressure increase of fluid in the third fluid line occurring thereby 38 opened as a result of adjusting pressure difference between the pump nearer side of the third consumer valve and the fluidkanalnäheren page. The one on the side of the third consumer pump 48 increased pressure moves the valve body of the third consumer valve 68 away from the valve seat until the valve body, for example, in the holding formation 80 , which is opposite to the valve seat in lifting direction A, comes into abutment. Then fluid from the third fluid supply 58 through the third supply fluid line 38 at the third supply valve 68 passing through a portion of the fluid channel 16 through the consumer valve 106 of the cell culture container just connected 63 passing through the consumer fluid line 108 through into the cell culture container 63 be initiated into it.

In the present case, for example, a ready-cultivated cell culture container 63 rinsed with PBS to remove nutrient media from those still in cell culture 63 remove existing adherent cells.

The switching magnet 124 may be an electromagnet which is energized or not energized if necessary. In the present example, it is the switching magnet 124 one at the housing 14 approachable and removable from this permanent magnet 124 which is not a heat source compared to an electromagnet. In 2 is with 126 that area bordered by a dashed line, which serves as a consumer switching point 126 the sphere of influence of the solenoid 124 shows.

In the consumer switching point 126 can by approaching the solenoid 124 to the housing 14 the fluid supply interface 10 a sufficiently strong magnetic field can be generated to the valve body of the consumer valve 106 against the action of its biasing magnet in the consumer valve 106 from its locking position in the in 2 adjusted flow position to adjust. Will the solenoid 124 back from the case 14 it will be removed in the switching location 126 acting magnetic field from the solenoid 124 emanating, so much attenuated that the attraction of the biasing magnet in the consumer valve 106 on the valve body of the consumer valve 106 outweighs and the valve body 106 back to the in 1 shifted shown locking position.

In 3 is now the situation of 2 shown, wherein the consumer valve 106 despite the approach of the solenoid 124 to the housing 14 and thus to the consumer switching point 126 erroneously not adjusted in the flow position. This error can have various causes, but it does not matter here. The switching magnet 124 may have experienced jamming and therefore may be incomplete to the housing 14 be approachable. It is also possible that it is between the valve body and the elastomeric material of the valve seat of the consumer valve 106 has come to unusually large clamping forces, so that of the solenoid 124 applied force is insufficient. Finally, the solenoid can 124 have lost their magnetization due to an external magnetic field.

In any case, if the third supply pump 48 is activated without the consumer valve 106 in its flow position, the pressure in the fluid channel 66 undesirably increase sharply. In this case, without further measures, it may happen that the fluid conveyed from the third fluid reservoir is attached to the fluid of the valve seat of the consumer valve 106 formed seal between the consumer feedback formation 128 and the consumer information 104 the supply fluid interface 10 Groundbreaking, along the between the consumer link formation 104 and the consumer feedback formation 128 existing gap exits and the operating room of the fluid supply interface 10 or in total the cell culture management plant 12 contaminated.

With the present embodiment of the fluid supply interface 10 This will not happen because of the valve body 98 of the first disposal valve 94 at sufficiently increased pressure in the fluid channel 16 and thus at a sufficiently high pressure difference between the fluid channel side and the disposal sink side of the first disposal valve 94 against the biasing force of the helical compression spring 102 opens and is adjusted in the flow position. With continued operation of the third supply pump 48 then fluid from the third fluid supply 58 directly into the disposal sink 90 promoted. At a dangerously high pressure build-up in the fluid channel 16 it does not come.

In 4 is the same state as in 3 shown, however, is the consumer valve 106 intended in its locking position and the first disposal valve 94 is by approaching a second switching magnet 130 at the first disposal valve 94 associated disposal switching point 132 specifically adjusted in the flow position. Thus, the force of the solenoid acts 130 on the valve body 98 of the first disposal valve 94 to overcome the biasing force of the helical compression spring 102 , This allows the fluid channel 16 in its section between the third supply valve 68 and the first disposal valve 94 with the third fluid supply 58 flush existing fluid before this fluid in the cell culture container 63 is initiated.

Due to the nature of the first disposal valve 94 as a mechanically biased pressure relief valve, there would be a switching operation of the first disposal valve 94 through the solenoid 130 not necessarily need. However, the targeted adjustment of the valve body 98 in the flow position through the solenoid 130 the switching device relative to the mere pressing of the first disposal valve 94 preferred by overpressure, since the valve operating position thus defined defined and not by the pressure and the spring reaction of the coil spring 102 is affected.

In 5 a state is shown in which the first disposal valve 94 through the solenoid 130 is adjusted in the flow position, and in which the valve body 74 of the first supply valve 64 by approach of a solenoid 134 the switching device in lifting direction A under contact with the holding formation 80 is adjusted in the passage position. The first supply valve 64 associated supply switching location 136 of the solenoid 134 is drawn in dashes.

This operating position of the supply interface 10 serves to clean the fluid channel 16 and all of these projecting valve bodies, including the valve body of a consumer valve 106 a coupled cell culture container 63 ,

In order to achieve the largest possible cleaning distance, preferably with the cleaning fluid supply 54 connected first supply coupling formation 20 and those with the disposal sink 90 connected first disposal coupling formation 82 arranged so that all other valves and valve body further coupling formations are located between them. In the present example, therefore, the distance of the first supply valve 64 from the first disposal valve 94 the largest at the fluid supply interface 10 between valves of two functionally different coupling formations existing distance.

By promoting cleaning fluid from the cleaning fluid supply 54 in the disposal sink 90 usually a sufficient cleaning of the fluid channel 16 and all achieved in these projecting valve components.

This cleaning effect can be achieved by the backwashing device 110 to be reinforced.

In the in 5 shown state was the fluid channel 16 initially filled with cleaning fluid, whereupon the shut-off valve 120 was operated so that only the backwash 110 , but not the first supply pump 44 with the fluid channel 16 connected is. By reciprocating the piston 16 in the cylinder 114 so can the in the fluid channel 16 spent cleaning fluid in opposite directions through the fluid channel 16 through and past all of these projecting valve components. The first supply valve 64 is completely by the backwash 110 caused reciprocal movement of the cleaning fluid rinsed.

Thus, the cleaning fluid unfolds not only a chemical cleaning action but also a mechanical one by repetitively flowing in opposite directions at one and the same location of a component. At the end of such a backwashing process, the backwash 110 through the shut-off valve 120 again from the fluid channel 16 can be disconnected and it can by activating the first supply pump 54 pure cleaning fluid finally through the fluid channel 16 through into the disposal sink 90 be directed. Through the shut-off valve 120 the cleaning fluid is in the first fluid supply 54 not affected by the backwashing.

The piston 116 For example, it can be driven by a rotating eccentric drive for reciprocating motion. Other drives are also conceivable.

From those at the supply interface 10 shown valves are in the example shown, only the first supply valve 64 , the consumer valve 106 , the first disposal valve 94 and the second disposal valve 96 by switching magnets 124 . 130 respectively. 134 switchable between their operating positions. For the second disposal valve 96 the solenoid is not shown.

The second to fifth supply valve 66 to 72 are exclusively through the second to fifth supply pump 46 to 52 from its blocking position in the passage position pressure-induced displaced. Thus, four switching magnets of a switching device are sufficient for the present fluid supply interface. This can be prevented that is undesirably more than one valve body is moved by a solenoid.

QUOTES INCLUDE IN THE DESCRIPTION

This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Cited patent literature

• DE 102013201069 A1 [0002]

Claims (19)

Fluid supply interface ( 10 ) for a cell culture plant, wherein the fluid supply interface ( 10 ): - at least one supply coupling formation ( 20 . 22 . 24 . 26 . 28 ) for coupling one supply fluid line ( 34 . 36 . 38 . 40 . 42 ) to the fluid supply interface ( 10 ), - at least one disposal coupling formation ( 82 . 84 ) for coupling one each of the supply fluid line ( 34 . 36 . 38 . 40 . 42 ) different disposal fluid line ( 86 . 88 ) to the fluid supply interface ( 10 ), - at least one consumer coupling formation ( 104 ) for coupling one each of the supply fluid line ( 34 . 36 . 38 . 40 . 42 ) and the disposal fluid line ( 86 . 88 ) different consumer fluid line ( 108 ) to the fluid supply interface ( 10 ), - a fluid channel ( 16 ), which contains the at least one supply coupling formation ( 20 . 22 . 24 . 26 . 28 ), the at least one disposal coupling formation ( 82 . 84 ) and the at least one consumer coupling formation ( 104 ), - at least one supply valve ( 64 . 66 . 68 . 70 . 72 ), by means of which, depending on its position, a supply coupling formation ( 20 . 22 . 24 . 26 . 28 ) can be flowed through by fluid or is blocked for a flow, - at least one of the supply valve ( 64 . 66 . 68 . 70 . 72 ) various disposal valve ( 94 . 96 ), by which, depending on its position, a disposal coupling formation ( 82 . 84 ) is traversed by fluid or is blocked for a flow, characterized in that the fluid supply interface with a backwash device ( 110 ), through which one and the same fluid in the fluid channel ( 16 ) is drivable to flow in opposite directions. Fluid supply interface according to claim 1, characterized in that the backwashing device ( 110 ) comprises a reversible pump, in particular a piston-cylinder arrangement ( 112 ). Fluid supply interface according to claim 2, characterized in that a backwash fluid line ( 122 ), which the direction reversible pump with the fluid channel ( 16 ) in the supply flow direction from the supply coupling formation ( 20 . 22 . 24 . 26 . 28 ) to the consumer link formation ( 104 ) upstream of the supply valve ( 64 . 66 . 68 . 70 . 72 ) is provided. Fluid supply interface according to one of the preceding claims, characterized in that the at least one supply valve ( 64 . 66 . 68 . 70 . 72 ), preferably biased by magnetic force in a flow-preventing blocking position and preferably by sufficiently high pressure difference between the two sides of the supply valve ( 64 . 66 . 68 . 70 . 72 ) against the biasing force to flow in the direction of the supply coupling formation ( 20 . 22 . 24 . 26 . 28 ) to the fluid channel ( 16 ), wherein the at least one disposal valve ( 94 . 96 ) is also biased in a closed position preventing its flow Fluid supply interface according to any one of the preceding claims, characterized in that the disposal valve ( 94 . 96 ) by changing an external magnetic field between its operating positions: closed position and flow position, is switchable, in particular a valve body ( 98 ) with or from ferromagnetic and / or permanent magnetized material and / or that at least one supply valve ( 64 . 66 . 68 . 70 . 72 ) by changing an external magnetic field between its operating positions: blocking position and passage position, is switchable, in particular a valve body ( 74 ) with or from ferromagnetic and / or permanent magnetized material. Fluid supply interface according to claim 5, characterized in that the at least one disposal valve ( 94 . 96 ) and the at least one supply valve ( 64 . 66 . 68 . 70 . 72 ) the same, preferably spherical, valve body ( 74 . 98 ) exhibit. Fluid supply interface according to one of the preceding claims, characterized in that a holding formation ( 80 ), which a valve body ( 74 ) of the at least one supply valve ( 64 . 66 . 68 . 70 . 72 ) in its passage position from a valve seat ( 100 ) lifted valve body ( 74 ) within the range of influence of the, preferably magnetic, prestressing force, in the fluid channel ( 16 ), in particular as a wall formation of the fluid channel ( 16 ), is trained. Fluid supply interface according to claim 7, characterized in that the flow cross-section of the fluid channel ( 16 ) in one to the holding formation ( 80 ) adjacent portion smaller than one to the trajectory of the fluid channel ( 16 ) in the adjacent section orthogonal cross-sectional dimension of the valve body ( 74 . 98 ), so that penetration of the valve body ( 74 . 98 ) in the fluid channel ( 16 ) is prevented. Fluid supply interface according to claim 7 or 8, characterized in that the fluid channel ( 16 ) with the lifting direction (A) of the valve body of at least one supply valve ( 64 . 66 . 68 . 70 . 72 ) and / or at least one disposal valve ( 94 . 96 ) includes an angle, preferably a right angle. Fluid supply interface according to one of the preceding claims, characterized in that a plurality of supply coupling information ( 20 . 22 . 24 . 26 . 28 ) is provided which - through the fluid channel ( 16 ) and - each one supply valve ( 64 . 66 . 68 . 70 . 72 ), which, preferably by magnetic force, is biased into a flow-preventing blocking position. Fluid supply interface according to one of the preceding claims, characterized in that the fluid supply interface ( 10 ) a switching device with at least one magnet ( 124 . 130 . 134 ), through which at a supply switching point ( 136 ) one of the at least one magnet ( 124 . 130 . 134 ) is variable to one at the supply switching point ( 136 ) provided supply valve ( 64 . 66 . 68 . 70 . 72 ) between its operating positions, the number of supply switching locations ( 136 ) is less than the number of supply valves ( 64 . 66 . 68 . 70 . 72 ). Fluid supply interface according to claim 11, characterized in that the at least one disposal valve ( 94 . 96 ) is switchable by the switching device between its operating positions. Fluid supply interface according to claim 12, characterized in that a plurality of disposal coupling information ( 82 . 84 ) is provided, which through the fluid channel ( 16 ) are connected to each other and each a disposal valve ( 94 . 96 ), wherein by the switching device at a disposal switching point ( 132 ) one of the at least one magnet ( 124 . 130 . 134 ) generated external magnetic field is variable to a disposal at the disposal ( 132 ) provided disposal valve ( 94 . 96 ) between its operating positions, the number of disposal switching locations ( 132 ) is equal to or less than the number of disposal valves ( 94 . 96 ). Fluid supply interface according to one of claims 11 to 13, characterized in that by the switching device also at a consumer switching point ( 126 ), which in the area of the consumer link formation ( 104 ) and at which a consumer fluid line ( 108 ) with a consumer valve ( 106 ) is temporarily disposable, one of the at least one magnet ( 124 . 130 . 134 ) is variable to the at the consumer switching point ( 126 ) provided consumer valve ( 106 ) between its operating positions: locking position and flow position, to be able to switch. Use of a fluid supply interface ( 10 ) according to one of the preceding claims for the management of cell culture containers, to each of which at least one consumer negative feedback formation ( 128 ) is provided for temporary coupling with the at least one consumer coupling formation. Cell culture management plant ( 12 ) with a fluid supply ( 54 . 56 . 58 . 60 . 60 ), with a disposal sink ( 90 ) and with a fluid supply interface ( 10 ) according to any one of claims 1 to 14, wherein the fluid supply ( 54 . 56 . 58 . 60 . 60 ) via a supply fluid line ( 34 . 36 . 38 . 40 . 42 ) with the supply coupling formation ( 20 . 22 . 24 . 26 . 28 ), the disposal sink ( 90 ) via a disposal fluid line ( 86 . 88 ) with the disposal coupling formation ( 82 . 84 ) and wherein a cell culture container ( 63 ) releasably connected to the at least one consumer coupling formation ( 104 ) can be coupled. Cell culture management plant ( 12 ) according to claim 16, characterized in that the backwashing fluid line ( 122 ) in the supply flow direction from the supply coupling formation ( 20 . 22 . 24 . 26 . 28 ) to the consumer link formation ( 104 ) upstream of the supply valve ( 64 . 66 . 68 . 70 . 72 ) into the supply fluid line ( 34 . 36 . 38 . 40 . 42 ) opens. Cell culture management system according to claim 17, characterized in that in supply flow direction of the supply coupling formation ( 20 . 22 . 24 . 26 . 28 ) to the consumer link formation ( 104 ) upstream of the mouth of the backwash fluid line ( 122 ) into the supply fluid line ( 34 . 36 . 38 . 40 . 42 ) a shut-off valve ( 120 . 120 ' ), by which a portion of the supply fluid line ( 34 . 36 . 38 . 40 . 42 ) from the backwashing fluid line ( 122 ) is separable. Cell culture management plant ( 12 ) according to one of claims 16 to 18 with a cleaning fluid supply as the fluid supply ( 54 ), with in addition at least one functional fluid supply ( 56 . 58 . 60 . 62 ) as another fluid supply ( 56 . 58 . 60 . 62 ), such as nutrient media stock ( 56 ), Flushing fluid supply ( 58 ) and solvent fluid reserve ( 60 ), and with an additional crop ( 92 ) as a further sink, wherein the cleaning fluid supply via a first supply fluid line ( 34 ) with a first supply coupling formation ( 20 ), wherein the at least one functional fluid reservoir ( 56 . 58 . 60 . 62 ) via a further supply fluid line ( 34 . 36 . 38 . 40 . 42 ) with another supply coupling formation ( 22 . 24 . 26 . 28 ), the disposal sink ( 90 ) via a first disposal fluid line ( 86 ) with a first disposal coupling formation ( 82 ) and wherein the crop counter ( 92 ) via a second disposal fluid line ( 88 ) with a second disposal coupling formation ( 84 ) is coupled.

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