DE102015204728B8 - Messvorrichtung, Mikrofluidikvorrichtung und Zellinkubationssystem zum Bestimmen einer Anzahl biologischer Zellen - Google Patents

Messvorrichtung, Mikrofluidikvorrichtung und Zellinkubationssystem zum Bestimmen einer Anzahl biologischer Zellen Download PDF

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Publication number
DE102015204728B8
DE102015204728B8 DE102015204728.8A DE102015204728A DE102015204728B8 DE 102015204728 B8 DE102015204728 B8 DE 102015204728B8 DE 102015204728 A DE102015204728 A DE 102015204728A DE 102015204728 B8 DE102015204728 B8 DE 102015204728B8
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DE
Germany
Prior art keywords
determining
biological cells
cell incubation
measuring device
incubation system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE102015204728.8A
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English (en)
Other versions
DE102015204728B3 (de
Inventor
Erik Jung
Leopold Georgi
Moritz Hubl
Martin Blechert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Technische Universitaet Berlin
Original Assignee
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Technische Universitaet Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV, Technische Universitaet Berlin filed Critical Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Priority to DE102015204728.8A priority Critical patent/DE102015204728B8/de
Application granted granted Critical
Publication of DE102015204728B3 publication Critical patent/DE102015204728B3/de
Publication of DE102015204728B8 publication Critical patent/DE102015204728B8/de
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486—Counting the particles
DE102015204728.8A 2015-03-16 2015-03-16 Messvorrichtung, Mikrofluidikvorrichtung und Zellinkubationssystem zum Bestimmen einer Anzahl biologischer Zellen Expired - Fee Related DE102015204728B8 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE102015204728.8A DE102015204728B8 (de) 2015-03-16 2015-03-16 Messvorrichtung, Mikrofluidikvorrichtung und Zellinkubationssystem zum Bestimmen einer Anzahl biologischer Zellen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102015204728.8A DE102015204728B8 (de) 2015-03-16 2015-03-16 Messvorrichtung, Mikrofluidikvorrichtung und Zellinkubationssystem zum Bestimmen einer Anzahl biologischer Zellen

Publications (2)

Publication Number Publication Date
DE102015204728B3 DE102015204728B3 (de) 2016-05-12
DE102015204728B8 true DE102015204728B8 (de) 2021-08-19

Family

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Family Applications (1)

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DE102015204728.8A Expired - Fee Related DE102015204728B8 (de) 2015-03-16 2015-03-16 Messvorrichtung, Mikrofluidikvorrichtung und Zellinkubationssystem zum Bestimmen einer Anzahl biologischer Zellen

Country Status (1)

Country Link
DE (1) DE102015204728B8 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102016217250A1 (de) * 2016-09-09 2018-03-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Mikrofluidische Vorrichtung für Zellkulturexperimente und Verwendungen hiervon
DE102017216581B4 (de) * 2017-09-19 2019-07-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Mikrofluidische Vorrichtung für Zellkulturexperimente und Verwendungen hiervon

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007038572A2 (en) 2005-09-26 2007-04-05 Massachusetts Institute Of Technology Microbioreactor for continuous cell culture
DE102006024355A1 (de) 2006-05-19 2007-11-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Mikrofluidische Anordnung zur Detektion von in Proben enthaltenen chemischen, biochemischen Molekülen und/oder Partikeln
DE102012013267A1 (de) 2012-07-04 2014-01-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Substrateinrichtung, Konservierungsgerät und Verfahren zur Kryokonservierung einer biologischen Probe
DE102013011768A1 (de) 2013-07-10 2015-01-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Zirkulationssystem sowie Verfahren zur Vitalversorgung von Zellkulturen in einem mikrofluidischen Netzwerk

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007038572A2 (en) 2005-09-26 2007-04-05 Massachusetts Institute Of Technology Microbioreactor for continuous cell culture
DE102006024355A1 (de) 2006-05-19 2007-11-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Mikrofluidische Anordnung zur Detektion von in Proben enthaltenen chemischen, biochemischen Molekülen und/oder Partikeln
DE102012013267A1 (de) 2012-07-04 2014-01-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Substrateinrichtung, Konservierungsgerät und Verfahren zur Kryokonservierung einer biologischen Probe
DE102013011768A1 (de) 2013-07-10 2015-01-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Zirkulationssystem sowie Verfahren zur Vitalversorgung von Zellkulturen in einem mikrofluidischen Netzwerk

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