DE102012217848A1 - Injection valve for sample injection device to inject fluid sample into measuring device, has sample supply line supplying fluid sample to port and attached with sample supply line, and port provided with sample receiving part and retainer - Google Patents

Abstract

The valve has a valve part provided with a first port and attached with fluid conduits. A first sample supply line supplies a fluid sample (90) i.e. liquid specimen, to a second port and attached with a second sample supply line. A third port is provided with a first sample receiving part and a retainer. A fourth port is attached with a second sample receiving part and the retainer. The first sample receiving part is fixed with a first sample measuring part (80). The second sample receiving part switches the fluid sample into a second sample measuring part (81). Independent claims are also included for the following: (1) a sample injection device (2) a measuring device (3) a method for injecting a fluid sample into a measuring device.

Description

TECHNICAL BACKGROUND

The present invention relates to an injection valve, a sample injection device, a measuring device and a sample injection method.

In an HPLC, a liquid (mobile phase) is typically run at a very precisely controlled flow rate (for example in the range of microliters to milliliters per minute) and at a high pressure (typically 20 to 1000 bar and beyond, currently up to 2000 bar). , in which the compressibility of the liquid is felt, moved by a stationary phase (for example, a chromatographic column) to separate individual components of a sample liquid introduced into the mobile phase from each other. Such an HPLC system is for example from EP 0,309,596 B1 by the same Applicant, Agilent Technologies, Inc.

For liquid chromatography, it is necessary to inject a fluid sample to be tested into the system. Such systems for injecting a fluid sample are out US 4,939,943 . US 3,916,692 or US 3,376,694 known.

Thus, in such and other meters, an injector may be provided for injecting a fluid sample into a path between a high pressure pump and a separation column. In such an injector loop, a needle may be disposed in a seat, wherein for receiving the fluid sample, the needle moves out of the seat, immersed in a sample vessel for aspirating the fluid sample and then retracts into the seat. After switching over an injection valve, the fluid sample thus accommodated is brought into the high-pressure path between the high-pressure pump and the separation column.

In an alternative concept, an injector needle can be operated seat-free by immersing it in a container with a liquid to be aspirated and then drawing the liquid through the injector needle into a fluid receiving loop for further processing.

Injection valves may include a stator with ports and a rotor with channels, which ports may be statically connected to fluid lines and the channels may be rotated with the rotor so as to fluidly couple different ports in different switch positions via a respective channel and others Fluidically decouple ports.

EPIPHANY

It is an object of the invention to provide an injection valve for loading samples in a measuring device, which can be used in a variety of ways. The object is achieved by means of the independent claims. Further embodiments are shown in the dependent claims.

According to an exemplary embodiment of the present invention, there is provided an injection valve for a sample injection device for injecting a fluid sample (in particular a liquid sample) into a meter, the injection valve having a first valve part with a plurality of ports for connecting fluid lines, a first one Port for connecting a first sample supply line for supplying the fluid sample is provided and a second port for connecting a second sample supply line for supplying the fluid sample is provided, wherein two third ports for connecting a first sample receiving device (in particular a first sample loop, "sample loop" ) are provided for receiving a first sample volume and two fourth ports are provided for connecting a second sample receiving device (in particular a second sample loop, "sample loop") for receiving a second sample volume, where a fifth port is provided for connecting a first sample meter to inspect the fluid sample, and a sixth port for connecting a second sample meter to inspect the fluid sample, and a second valve member having a plurality of channels for coupling respective ones of the ports a respective switching position of the injection valve, wherein the ports (and optional channels) of the first valve member and the channels of the second valve member by means of switching the injection valve relative to each other are fluidically coupled such that in a first switching position fluid sample from the first sample supply into the first sample receiving device is transferable, in a second switching position fluid sample from the first sample receiving device into the first sample measuring device can be transferred, in a third switching position fluid sample from the second sample supply into the second Probenaufn can be transferred, and in a fourth switching position fluid sample from the second sample receiving device into the second sample measuring device can be transferred.

According to another exemplary embodiment, there is provided a sample injection apparatus for injecting a fluid sample, the sample injection apparatus having a first sample supply line for supplying the fluid sample, a second sample supply line for supplying the fluid sample, a first sample receiver Receiving a first sample volume, a second sample receiving device for receiving a second sample volume and an injection valve having the features described above, wherein the first sample supply line is connected to the first port, the second sample supply line is connected to the second port, the first sample receiving device to the two third port is connected and the second sample receiving device is connected to the two fourth ports.

According to yet another exemplary embodiment, a measuring device for examining a fluid sample is provided, the measuring device selectively having a sample injection device with the features described above for injecting the fluid sample into the first sample receiving device or into the second sample receiving device, a first sample measuring device Examining the fluid sample is set up and which is for injecting the fluid sample received in the first sample receiving means in the first sample measuring means by means of the injection valve with the first sample receiving means coupled, and a second sample measuring device which is adapted to examine the fluid sample and which can be coupled to the second sample measuring device by means of the injection valve with the second sample receiving device for injecting the fluid sample received in the second sample receiving device into the second sample measuring device.

According to yet another exemplary embodiment, there is provided a method of injecting a fluid sample into a meter, wherein in the method, a first port of a first valve portion of an injection valve is connected to a first sample supply line for delivering the fluid sample, a second port of the first Valve part is connected to a second sample supply line for supplying the fluid sample, two third ports of the first valve part are connected to a first sample receiving device for receiving a first sample volume, two fourth ports of the first valve part are connected to a second sample receiving device for receiving a second sample volume a fifth port of the first valve member is connected to a first sample meter for inspecting the fluid sample, and a sixth port of the first valve member is connected to a second sample meter for inspecting the fluid sample. The ports (and optional channels) of the first valve member and a plurality of channels of a second valve member of the injection valve, which are adapted to couple respective ports according to a respective switching position of the injection valve, can be fluidly coupled by switching the injection valve relative to one another such that the arrangement can be operated in different operating modes. According to a first operating mode (for example a preparative operating mode), fluid sample can be transferred from the first sample supply line into the first sample receiving device in a first switching position, and fluid sample can be transferred from the first sample receiving device into the first sample measuring device in a second switching position. According to a (alternative or supplementary performed) second operating mode (for example, an analytical operating mode) can be transferred in a third switching position fluid sample from the second sample supply into the second sample receiving device and can in a fourth switching position fluid sample from the second sample receiving device in the second sample measuring device to be transferred.

According to an exemplary embodiment of the invention, there is provided an injection valve comprising, in a sample injecting device for injecting a fluid sample into a meter, two different sample supply lines for supplying fluid sample from a sample container, two different sample receiving devices for latching a fluid supplied from the respective sample supply line. Serve sample and two different sample measuring devices by switching the injection valve and can accomplish all the desired fluidic couplings and fluidic decoupling. A valve can thus jointly supply two (or more, for example, for the development of chromatographic methods) differently dimensioned or scaled sample measuring devices with fluid sample. For example, the two sample measuring devices can operate on the same principle - in particular according to the principle of liquid chromatography - but have different applications with different volumes of fluid samples to be processed. The two sample measuring devices may be fluidic paths in which the fluid sample cached in the respective sample receiving device is measured, analyzed or separated. Controlling the sample supply in two such fluidic paths through a common injection valve is particularly advantageous if a measuring arrangement is to be used on the one hand for analytical investigations and on the other hand for preparative investigations. For example, it may be desirable to subject a very low volume fluid sample to analytical testing, for example, separation, to analyze the sample. In contrast, a preparative study, which is often carried out after an analytical examination, have the object to process a very large volume of the fluid sample, in particular to separate. According to the invention, the injection valve provided in various In a first mode of operation (eg, a preparative mode of operation with a first throughput of the fluid sample), first loading the first sample receiver with a fluid sample from the first sample supply line and injecting that injection valve in the first Sample receiving device cached fluid sample in the associated first sample measuring device performs. In an alternative second mode of operation (eg, an analytical mode of operation having a second flow rate of the fluid sample which may be less than the first flow rate), loading of the second sample receiver with fluid sample from the second sample delivery line and switching of the injection valve may be initially initiated Inject the fluid sample temporarily stored in the second sample receiving device into the associated second sample measuring device. This dual function is achieved by the coupling logic of the fluidic ports with respect to the fluidic channels, which are formed in two corresponding valve parts.

In the following, additional embodiments of the injection valve, the sample injection device, the measuring device and the method will be described.

According to one exemplary embodiment, the sample measuring devices (together with the ports and channels of the injection valve connected thereto) operate at a higher pressure than the sample receiving devices or the sample supply lines (including the ports and channels of the injection valve connected thereto). Thus, the injection valve operates between a pressure gradient, in particular a pressure drop of at least 100 bar.

Individual fluid passages may be connected to the fluidic ports (which may be expanded in a surface area of the first valve part connected to the channels in order to achieve a desired fluid coupling in certain angular positions of the valve parts relative to one another), which are preferably provided in a stator valve part. By providing a corresponding switching position of the injection valve (ie, a relative position between the Statorventilteil and the rotor valve member) by the preferably provided in a rotor valve member, the fluidic coupling of the respective ports through a respective channel can be such that the operation of the two measurement paths by a single injection valve is possible.

According to an embodiment, the fifth port of the plurality of ports may be provided for connection to the first sample meter configured to inspect the fluid sample and for injecting the fluid sample received in the first sample receptacle into the first sample meter with the first sample receptacle can be coupled. The sixth port of the plurality of ports may be provided for connection to the second sample measuring device configured to inspect the fluid sample and coupled to the second sample receiving device for injecting the fluid sample received in the second sample receiving device into the second sample measuring device. Thus, the respective sample measuring device can be connected to the fifth and the sixth path. This may, for example, be a chromatographic separation path from a pump for pumping a mobile phase, a chromatographic separation column for separating different fractions of the fluid sample in the mobile phase and a detector for detecting the individual fractions of the fractions separated from the chromatographic separation column.

According to one embodiment, a seventh port of the plurality of ports may be provided for connection to a first fluid delivery device for delivering the fluid sample into the first sample receiving device. An eighth port of the plurality of ports may be provided for connection to a second fluid delivery device for delivering the fluid sample into the second sample receiving device. Thus, a respective port may be formed for coupling a respective injector pump for drawing the fluid sample into the respective sample receiving device and for subsequently conveying the drawn-in fluid sample further into the respective sample measuring device. For example, the respective fluid delivery device may be a syringe pump, peristaltic pump or another low-pressure pump that can be operated both in the direction of pull and in the direction of pressure.

According to one embodiment, at least one ninth port of the plurality of ports may be provided for connection to at least one first waste container for receiving fluid delivered by the sample injection device. Such a waste container may be for receiving fluids, for example, solvents, rinse fluid, or fluid samples that have been pumped through the ports, fluidic channels, and fluid communication lines of the sample injection device and are no longer needed. For example, a first waste container or waste path may be provided for the path from the first fluid supply line, first sample receiver, and the corresponding fluidic paths between ports and channels, and a second waste container for the fluid path from the second fluid supply line, second sample receiver, and associated ports and channels be provided. The waste containers or Wastepfade can be at atmospheric pressure and therefore can also be used for pressure relief of the injection valve, for example, when the injection valve is connected between a high pressure path at the respective sample measuring device and a lower pressure at the respective sample receiving device.

According to one embodiment, at least one tenth port of the plurality of ports may be provided for connection to at least one rinse pump for rinsing the sample injection device. Again, preferably, a first rinsing pump for rinsing a first fluidic path and a second rinsing pump for pumping a second fluidic path may be provided. The rinsing of the fluidic paths is particularly important because different fluid samples can be used in the two sample measuring devices operated by the injection valve, and different fluid samples can also be successively examined in one of the sample measuring devices. By rinsing, undesired sample carryover between different measurements can be avoided or reduced in the mentioned and other cases by cleaning the fluid lines inside and outside the injection valve by performing a rinsing operation.

According to one embodiment, the ports (and optionally channels) of the first valve member and the channels of the second valve member may be configured to be switchable relative to each other such that in a fifth shift position, a first portion of the sample injection device can be purged by conveying a purging fluid, and in a sixth shift position second portion of the sample injection device is flushable by conveying a flushing fluid. According to this embodiment, efficient flushing of adjustable fluidic paths in fluid communication with the injection valve can be enabled. For example, the fifth switching position may be taken after a fluid sample has been conveyed through the first sample delivery line and the first sample receiving device into the first sample measuring device and before handling another fluid sample from the second sample feeding line into and from the second sample receiving device the second sample measuring device is performed. The sixth shift position may be taken after a fluid sample has been conveyed through the second sample delivery line and the second sample receiving device into the second sample measuring device to prepare the system for subsequent sample processing.

According to one embodiment, the first portion may include a fluidic path from the first sample delivery line through at least two of the ports and through at least one port coupling these ports to a first purge fluid delivery device. Alternatively or additionally, the second portion may include a fluidic path from the second sample delivery line through at least two of the ports and through at least one channel coupling these ports to a second rinse fluid delivery device.

According to one exemplary embodiment, the ports (and optionally channels) of the first valve part and the passages of the second valve part may be configured to be switchable relative to one another in such a way that a pressure relief takes place at a transition between a first coupling position and a second coupling position. The first coupling position corresponds to a coupling of one of the sample receiving devices with an associated high-pressure sample measuring device. The second coupling position corresponds to a decoupling of this sample receiving device from this sample measuring device, which is now at a lower pressure. When the sample injection valve interfaces with one of the sample loops and one of the chromatographic sample separation paths, transferring sample from that sample loop into this chromatographic separation path (between the high pressure pump and the chromatographic separation column) results in a high pressure surge. This can conventionally lead to damage or destruction of the external components, in particular of the injection valve and / or of hoses or the like. According to the described embodiment, during the switching operation between a respective sample receiving device and a respective sample measuring device via a valve port, a valve channel and a restriction or damping pressure relief takes place, the components of the apparatus can be protected from unwanted damage by excessive pressure surges.

According to one exemplary embodiment, the pressure can be relieved by sliding off one of the channels, in particular a hammer-shaped channel, via a pressure relief port of the injection valve, with which the high pressure can be degraded, for example via a restriction (fluidic resistance) or a fluidic damping member. Thus, during the switching between the first and the second coupling position, preferably a channel of the second valve part can slide over a correspondingly formed port of the first valve part in order to reduce a high pressure via a fluidic impedance during this sliding operation. Excessive pressure surges can thus be damped. When Restriction may be used, for example, a bottleneck or the like.

In one embodiment, the first valve member and the second valve member may be rotatable relative to one another to selectively fluidly couple individual ones of the channels to associated ones of the respective ports. The design of the injection valve as a rotary valve is particularly advantageous, since a rotational shift assignment leads to a total of short switching paths and times and a simple operating logic. According to another embodiment, switching may be accomplished by translational movement between the first valve member and the second valve member.

According to one embodiment, the second sample volume may be smaller than the first sample volume. For example, according to this embodiment, the injection valve may serve to selectively operate an analytical and a preparative path. In the analytical mode of operation, which may be associated with the smaller sample volume, the fluid sample may be subjected to analysis. In contrast, in the preparative mode of operation, which may be associated with a larger sample volume, a larger amount of fluid may be processed.

According to one embodiment, the sample injection device may include a sample injection needle for injecting the fluid sample, the sample injection needle having a needle body, a first needle lumen having a first cross-sectional area for passing the fluid sample formed in the needle body, and a second needle lumen having a second Cross-sectional area for performing the fluid sample, which is formed in the needle body. Optionally, the second cross-sectional area (especially at a fluid receiving end of the needle body) may be smaller than the first cross-sectional area. A single such sample injection needle may be selectively coupled or coupled by means of the injection valve with the first sample receiving device or with the second sample receiving means, so that selectively fluid sample through the first needle lumen into the first sample receiving device is transferred or alternatively fluid sample through the second needle lumen in the second sample receiving device is transferable. In the meantime, the other needle lumen can be deactivated so that no fluid sample can be transported. Thus, according to the described embodiment, the sample injection needle, which can dip into a sample container for receiving fluid sample, have two lumens of different cross-sectional area. Then, by handling only a single sample injection needle, fluid sample can be selectively delivered through the first needle lumen into the first sample injection line or through the second needle lumen into the second sample injection line. The provision of such a double-lumen (or generally multi-lumen, i.e., three or more needle lumens may also be formed in the sample injection needle) injection needle allows a compact design of the sample injection device.

As an alternative to the provision of such a dual needle, however, two separate sample injection needles may be provided, from which a first sample injection needle can be immersed in a sample container for supplying the fluid sample through a first needle lumen into the first sample supply line. A separate second sample injection needle may be configured to deliver fluid sample from a sample container through a second needle lumen into the second sample delivery line.

In one embodiment, the injection valve may be the only injection valve of the sample injection device in a fluidic path between the sample delivery lines and the sample meters. In other words, in this embodiment, apart from the described injection valve, no further injection valve is provided between the sample supply lines, the sample receiving devices and the connecting lines of the sample measuring devices. The fact that the injection valve alone accomplishes all necessary fluidic coupling and decoupling processes, the entire arrangement can be made extremely compact.

According to one embodiment, the sample injection device may include a first fluid delivery device for delivering the fluid sample through the first needle lumen of the sample injection needle into the first sample receiving device and a second fluid delivery device for delivering the fluid sample through the second needle lumen of the sample injection needle into the second sample receiving device. A separate syringe pump can be provided as a fluid delivery device per fluidic path in order to transfer the fluid sample from a sample container through the respective needle lumen into the respective sample receiving device.

According to one exemplary embodiment, the injection valve may be switchable such that the first fluid delivery device can be coupled to a first sample measuring device of the measuring device by means of the injection valve such that the fluid sample can be transferred from the first sample receiving device into the first sample measuring device by means of the first fluid delivery device. The injection valve may also be switchable such that the second fluid delivery device by means of the injection valve with a second sample measuring device of the measuring device so it can be coupled that the fluid sample can be transferred from the second sample receiving device into the second sample measuring device by means of the second fluid conveying device. The respective fluid delivery device may be connected to a different port of the injection valve than the associated fluid supply line, the associated sample receiving device and the associated sample measuring device.

In one embodiment, the sample injection device may be a needle-seat-free sample injection device. According to this embodiment, the fluid sample can be sucked through the injection needle into the respective sample receiving device and be injected from there directly through the injection valve in the sample measuring device without the injection needle is previously inserted into a seat and the drawn into the sample receiving device fluid sample is again promoted by the needle lumen and a fluid line in the seat. Such a needle-seat-free configuration can provide a compact and simply constructed sample injection device that is immune to undesirable sample carryover.

Alternatively, however, a needle seat may be provided into which the sample injection needle is insertable to inject already stored in the associated sample receiving device fluid sample through the needle lumen, a fluid line of the needle seat and a port and a corresponding channel of the fluid valve in the associated sample measuring device.

According to one embodiment, the first sample receiving means may be configured to receive a first sample volume having a volume range of between about 1 ml and about 100 ml, more preferably in a volume range between about 5 ml and about 50 ml. According to an exemplary embodiment, the second sample receiving device may be designed to receive a second sample volume in a volume range between approximately 10 μl and approximately 1 ml, in particular in a volume range between approximately 50 μl and approximately 500 μl. The first sample volume can thus be tailored to the requirements of a preparative measuring path, whereas the second sample volume can be tailored to the requirements of an analytical measuring path.

According to one embodiment, the sample injection device may be pressure-resistant configured for operation at a pressure of up to about 100 bar, in particular for operation at a pressure of up to about 500 bar, more particularly for operation at a pressure of up to about 1000 bar. Such high pressures used in modern liquid chromatography applications (particularly HPLC applications) can withstand the sample injection device of the present invention. In order to minimize wear of the sample injection valve and thus pressure coupled components, such a design can implement pressure relief during a switching process between a high pressure path and a low pressure path to dampen excessive pressure surges and reduce excessive or abrasive flow rates.

According to one exemplary embodiment, at least one of the first sample measuring device and the second sample measuring device may have a sample separation path for separating different fractions of the fluid sample, in particular a chromatographic sample separation path, more particularly a liquid chromatographic sample separation path. Thus, the fluid sample, which has previously been drawn through a sample supply line in one of the sample receiving devices, after being coupled into the respective sample measuring device of a separation into different fractions can be subjected. In this case, preferably a chromatographic separation process can be used.

According to an embodiment, the first sample measurement device and / or the second sample measurement device may comprise a separation column for separating different fractions of the injected fluid sample. Such a separation column may be filled with an adsorption medium, for example porous beads of silica gel or activated carbon. By chemical interaction with these porous beads, the fluidic sample can then be temporarily immobilized or adsorbed on the separation column. For example, by adjusting a gradient of a solvent composition, the individual fractions can then be individually detached or desorption from the adsorption medium and subsequently detected.

According to an exemplary embodiment, the first sample measuring device and / or the second sample measuring device may have a pump for conveying the injected fluid sample together with a mobile phase. The mobile phase may be a solvent composition which may be constant in time or adjustably varied and which is mixed with the fluid sample after injection of the fluid sample through the injection valve into the sample separation path. The mixture of mobile phase and fluid sample may then be pumped through a high pressure pump through the chromatographic separation path to a separation column. Thus, the meter may include one or more pumps for carrying the injected fluid sample along with a mobile phase by at least a part of the measuring device. For example, such a pump may be configured to pump the mobile phase through the system at a high pressure, for example, from a few hundred bars up to 1000 bars or more.

According to one embodiment, the measuring device may be configured as a microfluidic measuring device, liquid chromatography device or HPLC. The measuring device can therefore be designed as a sample separation device and in particular as an HPLC device (High Performance Liquid Chromatography or High Performance Liquid Chromatography), a life science device or an SFC device (Supercritical Fluid Chromatography). However, other applications are possible.

According to one embodiment, the meter may include a sample detector for detecting separate components of the fluid sample. Such a sample detector may be based on a detection principle that detects electromagnetic radiation (for example in the UV range or in the visible range) originating from certain components of the fluid sample.

Alternatively or additionally, the meter may include a sample fractionator for fractionating the separated components. Such a fractionator may carry the various components, for example, into different liquid containers. The analyzed fluid sample can also be fed to a waste container.

According to one embodiment, the individual fluidic components (pump, separation column, detector, fractionator, etc.) for the first sample measuring device can be provided a second time separately for the second sample measuring device. As a result, it is possible to adapt these components to the requirements of the particular sample measuring device, in particular in terms of dimensions. Alternatively, however, one or more of these components (eg, pump or detector) may be common to both sample meters, i. H. only once, be provided. This can provide a compact meter.

BRIEF DESCRIPTION OF THE FIGURES

Other objects and many of the attendant advantages of embodiments of the present invention will be readily appreciated and become better understood by reference to the following more particular description of embodiments taken in conjunction with the accompanying drawings. Features that are substantially or functionally the same or similar are given the same reference numerals.

1 shows an HPLC meter according to an exemplary embodiment of the invention.

2 to 7 show a sample injection device with a sample injection valve and a sample injection needle according to an exemplary embodiment of the invention in six different switching states during the handling of a fluid sample in a preparative measurement path or in an analytical measurement path.

8th shows a sample injection needle with a dual lumen for a sample injection device according to an exemplary embodiment of the invention.

9 shows a sample injection needle with a dual lumen for a sample injection device according to another exemplary embodiment of the invention.

10 shows a dual needle for a sample injection device according to an exemplary embodiment of the invention in combination with two monolateral seating devices.

11 shows a duallum injection needle for a sample injection device according to an exemplary embodiment of the invention in combination with a Duallumensitzeinrichtung.

12 shows a dreilumige Probeninjektionsnadel for a sample injection device according to an exemplary embodiment of the invention.

The illustration in the drawing is schematic.

1 shows the structure of an HPLC system 10 according to an embodiment of the invention, for liquid chromatographic separation of fractions of a fluid sample 90 can be used.

The HPLC device 10 used to examine the fluid sample 90 and assists in separating the fluid sample 90 optionally in a first sample measuring device 80 , which corresponds to a preparative sample separation path, or in a second sample measuring device 81 corresponding to an analytical sample separation path. The sample measuring equipment 80 . 81 differ significantly in terms of the sample volume to be processed and thus the dimensioning of their individual components, but are functionally according to 1 otherwise trained accordingly. Alternatively, the individual sample injection devices 80 . 81 also completely be designed differently, for example, different separation mechanisms for separating fractions of the fluid sample 90 deploy.

The fluid sample to be examined 90 can in a sample container 92 be included with a membrane 94 can be closed sterile. A duallum sample injection needle 800 according to an exemplary embodiment of the invention, which in 8th can be described in more detail, can through the membrane 94 in the sample container 92 penetrate and into the fluid sample 90 plunge. An in 1 Sampling pump, not shown, may be the fluid sample 90 through a desired one of two needle lumens of the sample injection needle 800 suck and in a respective one of two sample loops (shown schematically with reference numerals 96 ) when a sample injection valve interposed therebetween 206 According to an exemplary embodiment of the invention is located in a corresponding switching state. The sample injection valve 206 is referred to 2 to 7 described in more detail. In particular, a volume of a fluid sample that is suitable for the first sample measuring device 80 is determined to be cached in a different sample loop than a volume of a fluid sample used for the second sample measuring device 81 is determined. Is the fluid sample 90 in the respective sample loop 96 cached, so this can at a corresponding switching state of the sample injection valve 206 selectively into the first sample measuring device 80 or in the second sample measuring device 81 be transferred.

In the following, the first sample measuring device will be exemplified 80 and how they work.

A pump 20 drives a mobile phase by a solvent container 25 provided and by means of a degasser 27 can be degassed by a separation device 30 (such as a chromatographic column) containing a stationary phase. A sample application unit 40 is between the pump 20 and the separation device 30 arranged to by means of the injection valve 206 the fluid sample 90 into the mobile phase. The stationary phase of the separation device 30 is intended to components of the fluid sample 90 to separate. A detector 50 Detects separated components of the fluid sample 90 , and a fractionating device 60 may be provided for separated components of the fluid sample 90 to spend, for example, in designated containers or a drain. A control unit 70 controls the components of the sample measuring device 80 ,

While a fluid path between the pump 20 and the separation device 30 typically at high pressure, the fluid sample becomes 90 Under normal pressure, first in a separate area, namely the respective sample loop 96 (Sample Loop), upstream of the sample unit 40 entered, the sample unit 40 then the fluid sample 90 into the high pressure liquid path. When connecting the initially under normal pressure fluid sample 90 in the sample loop 96 in the high pressure liquid path becomes the contents of the sample loop 96 abruptly (typically in the range of milliseconds) to the system pressure of the sample measuring device 80 brought.

The sample measuring device 81 is in the described embodiment quite analogous to the sample measuring device 80 constructed and differs only in terms of scaling or dimensioning (sample measuring device 81 processes much less fluid sample 90 per unit time as a sample measuring device 81 ). The sample measuring device 81 is capable of separating the fluid sample to be separated 90 in another switching state of the injection valve 206 to get fed. reference numeral 26 corresponds to reference character 25 , Reference number 28 corresponds to reference numeral 27 , Reference number 21 corresponds to reference numeral 20 , Reference number 41 corresponds to reference numeral 40 , Reference number 31 corresponds to reference numeral 30 , Reference number 51 corresponds to reference numeral 50 , Reference number 61 corresponds to reference numeral 60 and reference numerals 71 corresponds to reference numeral 70 ,

The components between the sample measuring device 81 and the sample measuring device 80 form a sample injection device 200 ,

In the following, reference is made to 2 such a sample injection device 200 for injecting the fluid sample 90 selectively into the first sample measuring device 80 or in the second sample measuring device 81 according to an exemplary embodiment of the invention.

1 shows the duallum sample injection needle 800 through its needle lumen, optionally in combination with an optional needle valve 270 , Fluid sample 90 either in a first sample supply line 214 or in a second sample delivery line 218 can be sucked. As will be described in more detail below, by means of the injection valve 206 one through the first sample delivery line 214 supplied fluid sample 90 in a first sample loop 202 as a first sample receiving means for receiving a first sample volume of the fluid sample 90 be transferred. In a corresponding manner, a means of the second Probenzuführleitung 218 supplied fluid sample 90 by means of the injection valve 206 in a second sample loop 204 be transferred as a second sample receiving means for receiving a second sample volume. In In the embodiment shown, the first sample volume is greater than the second sample volume. The first sample volume in the first sample loop 202 is stored for transfer to the preparative Probenmesspfad, that is, the first sample measuring device 80 educated. Similarly, fluid sample 90 that in the second sample loop 204 is cached with the lower receiving volume, provided for separation in the analytical separation path, that is, for separation in the second sample measuring device 81 intended.

Referring to below 8th will have the duallum sample injection needle 800 a larger and a smaller needle lumen. The larger needle lumen becomes the fluid sample 90 in the first sample loop 202 promoted with the larger recording volume. The smaller needle lumen becomes the fluid sample 90 into the second sample loop 204 promoted with the smaller receiving volume. The appropriate fluidic coupling for these sample delivery procedures is the combination of providing the dual lumen sample injection needle 800 and a corresponding configuration and switching of the injection valve 206 reached.

As in a detail 277 in 2 has the injection valve shown there in a schematic cross-sectional view 206 a first valve part 252 with a plurality of ports 272 for connecting fluid lines 274 on. The first valve part 252 is designed as a stator and can optionally also have channels that with a second valve part 250 can interact. In contrast, the second valve part 250 that with a plurality of channels 232 is provided (only one is in detail 277 shown) for fluidly coupling respective ports 272 according to a respective switching position of the injection valve 206 educated. The second valve part 250 is formed as a rotor which is rotatably actuated and thus with respect to the static first valve member 252 can be converted into a plurality of different switching positions. As a result, get in each switch position other of the ports 272 in fluid communication with a respective one of the channels 232 ,

A schematic plan view in FIG 2 shows the ports 272 and the channels 232 in the present switching state, in which a part of the ports 272 by means of a respective one of the channels 232 are fluidically coupled and another part of the ports 272 are fluidically decoupled or isolated.

A first port 212 of the first valve part 252 is for connecting the first sample supply line 214 for supplying the fluid sample 90 intended. A second port 216 is for connecting the second sample supply line 218 for supplying the fluid sample 90 intended. Between two third ports 220 . 222 is the first sample loop 202 for receiving the first sample volume of fluid sample 90 connected. Similarly, there are two fourth ports 224 . 226 for connecting the second sample loop 204 provided for receiving the second sample volume. A fifth port 228 serves to connect the first sample measuring device 80 for examining the fluid sample 90 , More precisely, to the fifth port 228 the sample application unit 40 according to 1 be connected. Similarly, a sixth port 230 for connecting the second sample measuring device 81 for examining the fluid sample 90 provided, more specifically, by means of the sixth port 230 the fluid valve 206 to the sample application device 41 connected. A seventh port 234 serves to connect a first syringe pump 208 (Alternatively, a peristaltic pump or other low-pressure pump) for drawing in the fluid sample 90 in the first sample loop 202 , An eighth port 236 serves to connect a second syringe pump 210 (Alternatively, a peristaltic pump or other low-pressure pump) for drawing in the fluid sample 90 into the second sample loop 204 ,

The first syringe or low pressure pump 208 corresponds fluidly with a first solvent / waste unit 276 , The second syringe or low pressure pump 210 corresponds fluidly with a second solvent / waste unit 278 ,

Two ninth ports 238 . 240 serve to connect a first waste container 280 or a second waste container 282 , The waste container 280 . 282 , which may be at atmospheric pressure, are for receiving by the sample injection device 200 pumped fluid that is no longer needed. Such a fluid can be, for example, a flushing fluid, but also a fluid sample 90 include.

Two tenth ports 242 . 246 serve to connect a first flushing pump 244 or a second flushing pump 248 , With the respective flushing pump 244 . 248 may be a respective path of the sample injection device 200 be rinsed. The first flushing pump 244 is with a solvent container 282 fluidly coupled, whereas the second flushing pump 248 with a second solvent container 284 is fluidically coupled.

Furthermore, the first valve part 252 a central port 288 in a center of the first valve part 252 ,

The various ports 272 can as circular cylindrical through holes in the first valve part 252 be educated. Single of the ports 272 are in a coupling area to the respective channels 232 on a surface of the first valve part 252 connected to the second valve part 250 adjacent, widened to allow certain fluidic couplings (see the elongated ports).

In 2 are the various ports of the first valve body 252 drawn by open structures, in particular circles and elongated ports. In contrast, the channels 232 shown as elongated thicker black lines. A hammer-shaped channel is denoted by reference numerals 254 Mistake.

In the following, reference is made to 2 to 7 six different switching positions of the sample injection valve 200 described.

In an in 2 shown first switching position is fluid sample 90 from the second sample supply line 218 into the second sample loop 204 transferred. This is the fluidic path of components 800 - 270 - 218 - 216 - 254 - 226 - 204 - 224 - 236 - 210 activated. The first switching position according to 2 corresponds to a zero degree position of the rotor or the second valve part 250 , According to the operating state shown becomes fluid sample 90 on the second sample loop 204 mounted and the sample injection device 200 otherwise operated in a bypass mode. The angle specifications described allow certain deviations or adjustments with respect to the particular desired function.

According to 2 is port 288 by means of a channel 232 fluidic with port 230 coupled. Furthermore, according to 2 port 212 by means of a channel 232 fluidic with port 242 coupled, port 236 by means of a channel 232 fluidic with port 224 coupled and port 216 by channel 254 fluidic with port 226 coupled.

In an in 3 shown second switching state is the fluid sample 90 from the second sample loop 204 in the second sample measuring device 81 transferred. For this purpose, a fluidic path of the components 288 - 232 - 224 - 204 - 226 - 230 closed. 3 corresponds to a switching position of the injection valve 206 of 45 ° (switching direction opposite 2 counterclockwise). Thus becomes fluid sample 90 injected on the analytical side and the sample injection device 200 otherwise operated in a restricted (needle and needle hose or line) rinse mode on the analytical side.

According to 3 is port 288 by means of a channel 232 fluidic with port 224 coupled. Furthermore, according to 3 port 246 by means of a channel 232 fluidic with port 216 coupled, and port 230 by channel 254 fluidic with port 226 coupled.

In an in 4 shown third switching state is a first portion of the sample injection device 200 rinsed by a flushing fluid. The switching position according to 4 corresponds to that according to 2 that is corresponds to an angle of 0 °. Now, solvent from the solvent container 282 through the first flushing pump 244 , as well as components 242 - 232 - 212 - 214 - 270 - 800 directed to rinse them. The operating state according to 4 can be referred to as purge and bypass mode. It may be the complete sample path 210 - 236 - 232 - 224 - 204 - 226 - 254 - 216 - 218 - 270 - 800 be rinsed during the ongoing analysis.

According to 4 is port 288 by means of a channel 232 fluidic with port 230 coupled. Furthermore, according to 4 port 212 by means of a channel 232 fluidic with port 242 coupled, port 236 by means of a channel 232 fluidic with port 224 coupled and port 216 by channel 254 fluidic with port 226 coupled.

In an in 5 shown fourth switching position of the injection valve 206 becomes fluid sample 90 from the first sample supply line 214 in the first sample loop 202 transferred. This corresponds to a fluidic coupling of the components 800 - 270 - 214 - 212 - 254 - 220 - 202 - 222 - 232 - 234 - 208 , The fourth switching position corresponds to an angular position of the fluid valve 206 of 180 °. In the switching position according to 5 becomes fluid sample 90 drawn for the preparative side and the injection valve 206 otherwise operated in bypass mode.

According to 5 is port 246 by means of a channel 232 fluidic with port 216 coupled. Furthermore, according to 5 port 212 by channel 254 fluidic with port 220 coupled, port 234 by means of a channel 232 fluidic with port 222 coupled and port 288 by channel 232 fluidic with port 228 coupled.

In an in 6 shown fifth switching position of the injection valve 206 becomes fluid sample 90 from the first sample loop 202 in the first sample measuring device 80 transferred. The operating state according to 6 corresponds to an angular position of 45 ° (counterclockwise to the fourth position). In the switching state shown becomes fluid sample 90 injected on the preparative side and the sample injection device 200 otherwise in a restricted (needle and needle hose or pipe) rinsing mode (needle and needle hose or pipe) operated on the preparative side.

According to 6 is port 288 by means of a channel 232 fluidic with port 222 coupled. Furthermore, according to 6 port 212 by means of a channel 232 fluidic with port 242 coupled and port 220 by channel 254 fluidic with port 228 coupled.

In an in 7 shown sixth switching position of the injection valve 206 Now becomes a second, different portion of the sample injection device 200 flushed by conveying a flushing fluid. According to 7 is a fluidic coupling between components 800 - 270 - 218 - 216 - 232 - 246 - 248 - 284 allowing these components through the second flushing pump 248 be rinsed. Furthermore, in this mode, the complete sample path 208 - 234 - 232 - 222 - 202 - 220 - 254 - 212 - 214 - 270 - 800 be rinsed during the ongoing analysis. The in 7 shown sixth switching state corresponds to an angular position of the injection valve 206 as in 5 ,

According to 7 is port 246 by means of a channel 232 fluidic with port 216 coupled. Furthermore, according to 7 port 212 by channel 254 fluidic with port 220 coupled, port 234 by means of a channel 232 fluidic with port 222 coupled and port 288 by channel 232 fluidic with port 228 coupled.

It should also be noted that the trained as a hammer channel channel 254 of the rotor or second valve part 250 advantageously a pressure relief of the injection valve 206 when switching between a coupling with the high pressure path (see Pumping 20 . 21 ) or one of them decoupled state allows. These are the ports 272 of the first valve part 252 and the channels 232 . 254 of the second valve part 250 formed so switchable relative to each other and arranged geometrically that at a transition between a coupling of one of the sample loops 202 . 204 with a respective high-pressure sample measuring device 80 . 81 to a decoupling of this sample loop 202 or 204 opposite this sample measuring device 80 or 81 a pressure relief takes place. Such a pressure relief takes place by sliding the hammer-shaped channel 254 via a pressure relief port of the injection valve 206 in a way that thereby the high pressure on a restriction and / or damping not shown in the figure and a waste container 280 . 282 or Waste path is degradable. This can reduce the wear on the injection valve 206 acts, be reduced.

Furthermore, according to 8th to 12 Multi-lumen sample injection needles 800 described in accordance with exemplary embodiments of the invention. These all each have several needle lumens in their interior. However, according to an alternative embodiment, it is possible to provide one or more single-lumen hypodermic needles.

8th shows a cross section of a Probeninjektionsnadel 800 for the sample injection device 200 for injecting the fluid sample 90 into the meter 10 ,

The sample injection needle 800 has a one-piece needle body 802 on. In the needle body 802 is a first needle lumen 804 with a first circular cross-sectional area here 808 for passing the fluid sample 90 educated. Further, in the needle body 802 a second needle lumen 806 with a here also circular second cross-sectional area 810 for passing the fluid sample 90 educated. As 8th can be taken, is the second cross-sectional area 810 smaller than the first cross-sectional area 808 , The first cross-sectional area 808 is over the entire needle body 802 constant in size and shape. Correspondingly, the second cross-sectional area is 810 over the entire needle body 802 constant in size and shape. Accordingly, the fluid sample 90 through the first needle lumen 804 promoted when the preparative injection path of the sample injection device 200 to be supplied with sample. In a similar way, the second needle lumen 806 used to fluid the analytical separation path 90 to supply. As 8th shows is the double-lumen hypodermic needle 800 via an optional fluid valve 270 optionally with the first sample loop 202 or with the second sample loop 204 coupled or coupled.

Alternatively, the first needle lumen 804 at the first cross-sectional area 808 defining (bottom) fluid receiving end of the needle body 802 have a first (for example conical) rejuvenation. Alternatively or additionally, the second needle lumen 806 at the second cross-sectional area 810 defining (bottom) fluid receiving end of the needle body 802 have a second (for example conical) rejuvenation. Then it can be inside the needle body 802 For example, connect a conical lumen end to a cylindrical lumen area. At the fluid receiving end can thus at least one of the needle lumens 804 . 806 opposite the rest of the respective needle lumen 804 . 806 have the narrowest point, which suppresses or reduces sample carryover.

The needle body 802 is according to 8th as a circular cylindrical body with a conical taper 812 at a fluid receiving end 814 educated. Thus, the sample injection needle is 800 a double-lobed circular cylinder with an end-shaped frusto-conical end section. This may cause dripping of absorbed fluid sample 90 promoted and consequently avoided or reduced unwanted sample carryover.

At the fluid receiving end 814 has the sample injection needle 800 partly a sharp cutting edge 816 , If, as in 1 shown schematically, the needle body 802 into the sample receptacle 92 dips to fluid sample 90 soak up the teilumfängliche cutting edge 816 a for example U-shaped cut in the membrane cover 94 perform, allowing a convenient immersion of the sample injection needle 800 into the fluid sample 90 can be accomplished.

As 8th shows is a first fluid line 818 fluid-tight to the first needle lumen 804 connected. A second fluid line 820 is fluid tight to the second needle lumen 806 connected. According to 8th are the first needle lumen 804 and the second needle lumen 806 in the needle body 802 arranged side by side. The first needle lumen 804 runs in the needle body 802 parallel to the second needle lumen 806 , The needle body 802 For example, it may be made of a ceramic or other bio-inert and abrasion-resistant material. Optionally, the first needle lumen can / can 804 and / or the second needle lumen 806 be tapered at least in sections, preferably at the bottom sample inlet.

9 shows a Probeninjektionsnadel 800 according to another exemplary embodiment of the invention. In contrast to 8th is according to 9 the needle valve 270 omitted. Thus, the first sample loop 202 with the first needle lumen 804 directly coupled. Similarly, the second sample loop 204 with the second needle lumen 806 directly coupled. 9 also shows dimensions of the needle body 802 , An outer diameter of the needle body 802 is here in its circular cylindrical section 3 mm. A diameter of the cross-sectionally circular first needle lumen 804 is in the illustrated embodiment 1.2 mm. The cross-sectionally circular second needle lumen 806 has a diameter of 0.3 mm in the illustrated embodiment.

10 shows an injection needle 800 which, in terms of their properties, comply with the 8th respectively. 9 shown corresponds. Further shows 10 a first seat device 1050 and a second seat device 1000 , In the first seating device is a seat lumen 1004 educated. Will the injection needle 800 into a recording area 1052 the first seat device 1050 introduced fluid-tight (see reference numeral 1066 ), this results in a fluid-tight connection between the second needle lumen 806 and the first seat lumen 1004 educated. On the other hand, there is no fluid communication through the first needle lumen 804 possible if the hypodermic needle 800 in the first seat device 1050 is introduced since the first needle lumen 804 in the first seat setup 1050 blind flanged.

As further in 10 shown has the second seat assembly 1000 such a arranged second seat lumen 1002 that when inserting the injection needle 800 in a recording room 1054 the second seat device 1000 (see reference numeral 1068 ) a fluid-tight fluid communication between the first needle lumen 804 and the second seat lumen 1002 is possible. By contrast, in this operating state, there is no fluid communication through the second needle lumen 806 possible.

Consequently, the multi-lumen injection needle 800 with two seating facilities 1000 . 1050 be used together. The sample injection needle 800 can be used to aspirate a fluid sample 90 in a sample loop into a sample container 92 immerse and can after sucking in the respective seat device 1050 or 1000 be introduced. The fluid sample aspirated into the sample loop 90 can then pass through a respective one of the seating devices 1050 respectively. 1000 be forwarded, for example, in a sample measuring device.

11 shows a doppelellumige injection needle 800 according to another exemplary embodiment of the invention. The injection needle 800 largely corresponds to the configuration according to 8th respectively. 9 , but still has an end-face shape recognition feature 1120 in the form of a V-shaped recess in this case, corresponding to one on a corresponding seat device 1100 provided corresponding shape recognition feature 1122 is shaped. The shape recognition feature 1122 is a V-shaped tip in the embodiment shown. Will the sample injection needle 800 in a recording 1126 the seat device 1100 introduced, so put together form a form-fitting forming recognition features 1120 . 1122 sure the injection needle 800 with respect to the seating device 1100 is introduced correctly. In other words, the shape recognition features 1120 . 1122 a twist protection ready. When the sample injection needle 800 correctly in the seat assembly 1100 is inserted, the first needle lumen is aligned 804 with a first seat lumen 1102 , At the same time, the second needle lumen is aligned 806 with a second seat lumen 1104 , Fluid sample 90 can then pass through the sample injection needle 800 in the desired seat lumen 1102 or 1104 be injected.

12 shows an injection needle 800 according to yet another embodiment of the invention. This can be like one of the 8th to 11 shown Probeninjektionsnadeln 800 be formed, but has a third needle lumen 1202 on. Thus, even a third fluid channel (or, if required, several further fluid channels) in the interior of the sample injection needle 800 be formed, which then serves as a multi-lumen injection needle.

It should be noted that the term "comprising" does not exclude other elements and that the "on" does not exclude a plurality. Also, elements described in connection with different embodiments may be combined. It should also be noted that reference signs in the claims should not be construed as limiting the scope of the claims.

QUOTES INCLUDE IN THE DESCRIPTION

This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Cited patent literature

• EP 0309596 B1 [0002]
• US 4939943 [0003]
• US 3916692 [0003]
• US 3376694 [0003]

Claims (17)

Injection valve ( 206 ) for a sample injection device ( 200 ) for injecting a fluid sample ( 90 ) into a measuring device ( 10 ), the injection valve ( 206 ): a first valve part ( 252 ) with a plurality of ports ( 272 ) for connecting fluid lines ( 274 ), wherein a first port ( 212 ) for connecting a first sample supply line ( 214 ) for supplying fluid sample ( 90 ) and a second port ( 216 ) for connecting a second sample supply line ( 218 ) for supplying fluid sample ( 90 ), whereby two third ports ( 220 . 222 ) for connecting a first sample receiving device ( 202 ) for receiving a first sample volume of fluid sample ( 90 ) and two fourth ports ( 224 . 226 ) for connecting a second sample receiving device ( 204 ) for receiving a second sample volume of fluid sample ( 90 ), whereby a fifth port ( 228 ) for connecting a first sample measuring device ( 80 ) for examining fluid sample ( 90 ) and a sixth port ( 230 ) for connecting a second sample measuring device ( 81 ) for examining fluid sample ( 90 ) is provided; and a second valve part ( 250 ) with a plurality of channels ( 232 ) for coupling respective ones of the ports ( 272 ) according to a respective switching position of the injection valve ( 206 ) having; where the ports ( 272 ), and optional channels, of the first valve part ( 252 ) and the channels ( 232 ) of the second valve part ( 250 ) by switching the injection valve ( 206 ) are fluidically coupled relative to each other such that: in a first switching position ( 5 ) Fluid sample ( 90 ) from the first sample supply line ( 214 ) into the first sample receiving device ( 202 ) is convertible; in a second switching position ( 6 ) Fluid sample ( 90 ) from the first sample receiving device ( 202 ) into the first sample measuring device ( 80 ) is convertible; in a third switching position ( 2 ) Fluid sample ( 90 ) from the second sample supply line ( 218 ) into the second sample receiving device ( 204 ) is convertible; in a fourth switching position ( 3 ) Fluid sample ( 90 ) from the second sample receiving device ( 204 ) into the second sample measuring device ( 81 ) is convertible. Injection valve ( 206 ) according to claim 1, wherein a seventh port ( 234 ) of the plurality of ports ( 272 ) for connection to a first fluid delivery device ( 208 ) for conveying the fluid sample ( 90 ) into the first sample receiving device ( 202 ) is provided; where an eighth port ( 236 ) of the plurality of ports ( 272 ) for connection to a second fluid delivery device ( 210 ) for conveying the fluid sample ( 90 ) into the second sample receiving device ( 204 ) is provided. Injection valve ( 206 ) according to claim 1 or 2, comprising at least one of the following features: at least one ninth port ( 238 . 240 ) of the plurality of ports ( 272 ) is for connection to at least one first waste container ( 280 . 282 ) for receiving by the sample injection device ( 200 ) provided fluid provided; at least a tenth port ( 242 . 246 ) of the plurality of ports ( 272 ) is for connection to at least one flushing fluid conveying device ( 244 . 248 ) for rinsing the sample injection device ( 200 ) intended; the first valve part ( 252 ) and the second valve part ( 250 ) are rotatable relative to each other to individual of the channels ( 232 ) with associated ports ( 272 ) to couple selectively fluidically. Injection valve ( 206 ) according to one of claims 1 to 3, wherein the ports ( 272 ) of the first valve part ( 252 ) and the channels ( 232 ) of the second valve part ( 250 ) by switching the injection valve ( 206 ) are fluidically coupled relative to each other such that: in a fifth switching position ( 4 ) a first portion of the sample injection device ( 200 ) is flushable by conveying a flushing fluid; in a sixth switching position ( 7 ) a second portion of the sample injection device ( 200 ) is flushable by conveying a flushing fluid. Injection valve ( 206 ) according to claim 4, wherein the first subregion comprises a fluidic path from the first sample supply line ( 214 ) through at least two of the ports ( 212 . 242 ) and through at least one of these ports ( 212 . 242 ) coupling channel ( 232 ) to a first flushing fluid conveying device ( 244 ) contains; and / or wherein the second subregion comprises a fluidic path from the second sample supply line ( 218 ) through at least two of the ports ( 216 . 246 ) and through at least one of these ports ( 216 . 246 ) coupling channel ( 232 ) to a second flushing fluid conveying device ( 248 ) contains. Injection valve ( 206 ) according to one of claims 1 to 5, wherein the ports ( 272 ) of the first valve part ( 252 ) and the channels ( 232 ) of the second valve part ( 250 ) by switching the injection valve ( 206 ) are fluidly coupled relative to each other such that at a transition between a coupling of one of the sample receiving devices ( 202 . 204 ) with a respective high-pressure sample measuring device ( 80 ; 81 ) and a decoupling of this sample receiving device ( 202 . 204 ) of this sample measuring device ( 80 . 81 ) a pressure relief takes place. Injection valve ( 206 ) according to claim 6, wherein the pressure relief by sliding of one of the channels ( 232 ), in particular a hammer-shaped channel ( 254 ), via a pressure relief port ( 272 ) of the injection valve ( 206 ), whereby the high pressure via the pressure relief port ( 272 ), and in particular a restriction and a waste container ( 280 . 282 ) or waste path, is degradable. Sample injection device ( 200 ) for injecting a fluid sample ( 90 ), the sample injection device ( 200 ): a first sample supply line ( 214 ) for supplying fluid sample ( 90 ); a second sample supply line ( 218 ) for supplying fluid sample ( 90 ); a first sample receiving device ( 202 ) for receiving a first sample volume of fluid sample ( 90 ); a second sample receiving device ( 204 ) for receiving a second sample volume of fluid sample ( 90 ); an injection valve ( 206 ) according to any one of claims 1 to 7; wherein the first sample supply line ( 214 ) to the first port ( 212 ), the second sample supply line ( 218 ) to the second port ( 216 ), the first sample receiving device ( 202 ) to the two third ports ( 220 . 222 ) and the second sample receiving device ( 204 ) to the two fourth ports ( 224 . 226 ) connected. Sample injection device ( 200 ) according to claim 8, wherein the second sample volume is smaller than the first sample volume. Sample injection device ( 200 ) according to claim 8 or 9, comprising a sample injection needle ( 800 ) for injecting the fluid sample ( 90 ), the sample injection needle ( 800 ) comprises: a needle body ( 802 ); a first needle lumen ( 804 ) having a first cross-sectional area ( 808 ) for carrying out the fluid sample ( 90 ), which in the needle body ( 802 ) is formed; a second needle lumen ( 806 ) having a second cross-sectional area ( 810 ) for carrying out the fluid sample ( 90 ), which in the needle body ( 802 ) is formed; in particular the second cross-sectional area ( 810 ) smaller than the first cross-sectional area ( 808 ); the sample injection needle ( 800 ) by means of the injection valve ( 206 ) selectively with the first sample receiving device ( 202 ) or with the second sample receiving device ( 204 ) is fluidically coupled or coupled, so that selectively fluid sample ( 90 ) through the first needle lumen ( 804 ) into the first sample receiving device ( 202 ) is transferable or fluid sample ( 90 ) through the second needle lumen ( 806 ) into the second sample receiving device ( 204 ) is convertible. Sample injection device ( 200 ) according to one of claims 8 to 10, wherein the injection valve ( 206 ) the only injection valve ( 206 ) of the sample injection device ( 200 ) in a fluidic path between the sample supply lines ( 214 . 218 ) and the sample measuring devices ( 80 . 81 ). Sample injection device ( 200 ) according to claim 10 or 11, comprising a first fluid conveying device ( 208 ) for conveying the fluid sample ( 90 ) through the first needle lumen ( 804 ) of the sample injection needle ( 800 ) into the first sample receiving device ( 202 ); comprising a second fluid delivery device ( 210 ) for conveying the fluid sample ( 90 ) through the second needle lumen ( 804 ) of the sample injection needle ( 800 ) into the second sample receiving device ( 204 ). Sample injection device ( 200 ) according to claim 12, wherein the injection valve ( 206 ) is switchable such that the first fluid conveying device ( 208 ) by means of the injection valve ( 206 ) with a first sample measuring device ( 80 ) of the measuring device ( 10 ) is coupled so that by means of the first fluid delivery device ( 208 ) the fluid sample ( 90 ) from the first sample receiving device ( 202 ) into the first sample measuring device ( 80 ) is convertible; and wherein the injection valve ( 206 ) is switchable such that the second fluid conveying device ( 210 ) by means of the injection valve ( 206 ) with a second sample measuring device ( 81 ) of the measuring device ( 10 ) is coupled so that by means of the second fluid conveying device ( 210 ) the fluid sample ( 90 ) from the second sample receiving device ( 204 ) into the second sample measuring device ( 81 ) is convertible. Sample injection device ( 200 ) according to one of claims 8 to 13, comprising one of the following features: the sample injection device ( 200 ) is a needle-seat-free sample injection device ( 200 ); the first sample receiving device ( 202 ) is designed to receive a first sample volume in a range between 1 ml and 100 ml, in particular in a range between 5 ml and 50 ml; the second sample receiving device ( 204 ) is designed to receive a second sample volume in a range between 10 .mu.l and 1 ml, in particular in a range between 50 .mu.l and 500 .mu.l; the sample injection device ( 200 ) is suitable for operation at a pressure of up to 100 bar, in particular for operation at a pressure of up to 500 bar, in particular for operation at a pressure of up to 1000 bar, pressure-resistant. Measuring device ( 10 ) for examining a fluid sample ( 90 ), whereby the measuring device ( 10 ): a sample injection device ( 200 ) according to any one of claims 8 to 14 for injecting fluid sample ( 90 ) selectively into the first sample receiving device ( 202 ) or in the second sample receiving device ( 204 ); a first sample measuring device ( 80 ) used to examine fluid sample ( 90 ) and for injecting in the first sample receiving device ( 202 ) recorded fluid sample ( 90 ) into the first sample measuring device ( 80 ) by means of the injection valve ( 206 ) with the first sample receiving device ( 202 ) can be coupled; a second sample measuring device ( 81 ) used to examine fluid sample ( 90 ) and that for injecting in the second sample receiving device ( 204 ) recorded fluid sample ( 90 ) into the second sample measuring device ( 81 ) by means of the injection valve ( 206 ) with the second sample receiving device ( 204 ) can be coupled. Measuring device ( 10 ) according to claim 15, comprising at least one of the following features: at least one of the first sample measuring device ( 80 ) and the second sample measuring device ( 81 ) has a sample separation path ( 30 ) for separating different fractions of the fluid sample ( 90 ), in particular a chromatographic sample separation path ( 30 ), more particularly a liquid chromatographic sample separation path ( 30 ), on; the first sample measuring device ( 80 ) has a preparative sample separation path and the second sample measurement device ( 81 ) has an analytical sample separation path; at least one of the first sample measuring device ( 80 ) and the second sample measuring device ( 81 ) has a separation column ( 30 ) for separating different fractions of the injected fluid sample ( 90 ) on; at least one of the first sample measuring device ( 80 ) and the second sample measuring device ( 81 ) has a pump ( 20 ) for conveying the injected fluid sample ( 90 ) together with a mobile phase; the measuring device ( 10 ) is set up as one of the group consisting of a microfluidic measuring device, a liquid chromatography device and an HPLC. Method for injecting a fluid sample ( 90 ) into a measuring device ( 10 ), wherein in the method: a first port ( 212 ) of a first valve part ( 252 ) of an injection valve ( 206 ) to a first sample supply line ( 214 ) for supplying fluid sample ( 90 ) is connected; a second port ( 216 ) of the first valve part ( 252 ) to a second sample supply line ( 218 ) for supplying fluid sample ( 90 ) is connected; two third ports ( 220 . 222 ) of the first valve part ( 252 ) to a first sample receiving device ( 202 ) for receiving a first sample volume of fluid sample ( 90 ) are connected; two fourth ports ( 224 . 226 ) of the first valve part ( 252 ) to a second sample receiving device ( 204 ) for receiving a second sample volume of fluid sample ( 90 ) are connected; a fifth port ( 228 ) of the first valve part ( 252 ) to a first sample measuring device ( 80 ) for examining fluid sample ( 90 ) is connected; and a sixth port ( 230 ) of the first valve part ( 252 ) to a second sample measuring device ( 81 ) for examining fluid sample ( 90 ) is connected; and the ports ( 272 ), and optional channels, of the first valve part ( 252 ) and a plurality of channels ( 232 ) a second valve part ( 250 ) of the injection valve ( 206 ), which channels ( 232 ) for coupling respective ones of the ports ( 272 ) according to a respective switching position of the injection valve ( 206 ) are arranged by means of switching the injection valve ( 206 ) are fluidly coupled relative to each other such that: in a first switching position ( 5 ) Fluid sample ( 90 ) from the first sample supply line ( 214 ) into the first sample receiving device ( 202 ) is transferred; and in a second switching position ( 6 ) Fluid sample ( 90 ) from the first sample receiving device ( 202 ) into the first sample measuring device ( 80 ) is transferred; and / or in a third switching position ( 2 ) Fluid sample ( 90 ) from the second sample supply line ( 218 ) into the second sample receiving device ( 204 ) is transferred; and in a fourth switching position ( 3 ) Fluid sample ( 90 ) from the second sample receiving device ( 204 ) into the second sample measuring device ( 81 ) is transferred.

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