DE102007038906A1 - Device for execution of electrophoresis experiment, has carrier material and electrically charged particles on side of substrate, where charged particles are detected - Google Patents
Device for execution of electrophoresis experiment, has carrier material and electrically charged particles on side of substrate, where charged particles are detected Download PDFInfo
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- DE102007038906A1 DE102007038906A1 DE102007038906A DE102007038906A DE102007038906A1 DE 102007038906 A1 DE102007038906 A1 DE 102007038906A1 DE 102007038906 A DE102007038906 A DE 102007038906A DE 102007038906 A DE102007038906 A DE 102007038906A DE 102007038906 A1 DE102007038906 A1 DE 102007038906A1
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- 239000000758 substrate Substances 0.000 title claims abstract description 23
- 239000002245 particle Substances 0.000 title claims abstract description 11
- 239000012876 carrier material Substances 0.000 title claims abstract description 6
- 238000001962 electrophoresis Methods 0.000 title claims description 25
- 238000002474 experimental method Methods 0.000 title claims description 12
- 239000011159 matrix material Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 11
- 230000003287 optical effect Effects 0.000 claims description 6
- 238000002601 radiography Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 description 7
- 238000000926 separation method Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 238000001155 isoelectric focusing Methods 0.000 description 3
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000005525 hole transport Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- PQXKHYXIUOZZFA-UHFFFAOYSA-M lithium fluoride Chemical compound [Li+].[F-] PQXKHYXIUOZZFA-UHFFFAOYSA-M 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920000301 poly(3-hexylthiophene-2,5-diyl) polymer Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229920000144 PEDOT:PSS Polymers 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- MCEWYIDBDVPMES-UHFFFAOYSA-N [60]pcbm Chemical compound C123C(C4=C5C6=C7C8=C9C%10=C%11C%12=C%13C%14=C%15C%16=C%17C%18=C(C=%19C=%20C%18=C%18C%16=C%13C%13=C%11C9=C9C7=C(C=%20C9=C%13%18)C(C7=%19)=C96)C6=C%11C%17=C%15C%13=C%15C%14=C%12C%12=C%10C%10=C85)=C9C7=C6C2=C%11C%13=C2C%15=C%12C%10=C4C23C1(CCCC(=O)OC)C1=CC=CC=C1 MCEWYIDBDVPMES-UHFFFAOYSA-N 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 239000002800 charge carrier Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005370 electroosmosis Methods 0.000 description 1
- 238000001997 free-flow electrophoresis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000767 polyaniline Polymers 0.000 description 1
- 229920002098 polyfluorene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000001853 pulsed-field electrophoresis Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
-
- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
- H10K30/00—Organic devices sensitive to infrared radiation, light, electromagnetic radiation of shorter wavelength or corpuscular radiation
- H10K30/30—Organic devices sensitive to infrared radiation, light, electromagnetic radiation of shorter wavelength or corpuscular radiation comprising bulk heterojunctions, e.g. interpenetrating networks of donor and acceptor material domains
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- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
- H10K39/00—Integrated devices, or assemblies of multiple devices, comprising at least one organic radiation-sensitive element covered by group H10K30/00
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- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
- H10K85/00—Organic materials used in the body or electrodes of devices covered by this subclass
- H10K85/10—Organic polymers or oligomers
- H10K85/111—Organic polymers or oligomers comprising aromatic, heteroaromatic, or aryl chains, e.g. polyaniline, polyphenylene or polyphenylene vinylene
- H10K85/113—Heteroaromatic compounds comprising sulfur or selene, e.g. polythiophene
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- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
- H10K85/00—Organic materials used in the body or electrodes of devices covered by this subclass
- H10K85/10—Organic polymers or oligomers
- H10K85/111—Organic polymers or oligomers comprising aromatic, heteroaromatic, or aryl chains, e.g. polyaniline, polyphenylene or polyphenylene vinylene
- H10K85/113—Heteroaromatic compounds comprising sulfur or selene, e.g. polythiophene
- H10K85/1135—Polyethylene dioxythiophene [PEDOT]; Derivatives thereof
-
- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
- H10K85/00—Organic materials used in the body or electrodes of devices covered by this subclass
- H10K85/20—Carbon compounds, e.g. carbon nanotubes or fullerenes
- H10K85/211—Fullerenes, e.g. C60
- H10K85/215—Fullerenes, e.g. C60 comprising substituents, e.g. PCBM
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Nanotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
Die vorliegende Erfindung betrifft ein Verfahren und eine Vorrichtung zur Detektion elektrophoretisch aufgetrennter Analyten.The The present invention relates to a method and an apparatus for the detection of electrophoretically separated analytes.
Elektrophorese bezeichnet die Wanderung elektrisch geladener Teilchen durch einen als Trägermaterial dienenden Stoff in einem elektrischen Feld. Zur Detektion elektrophoretisch aufgetrennter Analyten werden bislang mehrere elektrische und optische Detektoren verwendet. Es gibt eine Reihe elektrophoretischer Trennmethoden. Beispielsweise gibt es folgende Arten der Elektrophoreseexperimente: Diskontinuierliche Elektrophorese; Gel-Elektrophorese, Kapillar-Elektrophorese, Gradienten-Elektrophorese, pulsed-Field-Elektrophorese, Dichtegradienten-Elektrophorese (trägerfreie Elektrophorese), Elektrofokussierung, Lipidelektrophorese, Lipidelektrophorese, Serumelektrophorese, zweidimensionale Elektrophorese, Free-Flow-Elektrophorese und Elektroosmose.electrophoresis refers to the migration of electrically charged particles through a as a carrier material serving stuff in an electric field. For detection electrophoretically Separated analytes are so far multiple electrical and optical detectors used. There are a number of electrophoretic separation methods. For example, there are the following types of electrophoresis experiments: Discontinuous electrophoresis; Gel electrophoresis, capillary electrophoresis, gradient electrophoresis, pulsed field electrophoresis, density gradient electrophoresis (Unsupported Electrophoresis), electrofocusing, lipid electrophoresis, lipid electrophoresis, Serum electrophoresis, two-dimensional electrophoresis, free-flow electrophoresis and electroosmosis.
Die wichtigsten qualitativen Methoden – Agarose-Gel-Elektrophorese, SDS-PAGE, isoelektrische Fokussierung – dienen zur Auftrennung von DNA und Proteinen. Zweidimensionale Elektrophorese (SDS-PAGE und isoelektrische Fokussierung) erlaubt höchstmögliche Auflösung mit Auftrennung der Analyte nach Ladung und Molekulargewicht. Bei der Gel-Elektrophorese spielt auch das Verhältnis zwischen dem Teilchenradius und der Porenweite des als Trägermedium dienenden Gels eine Rolle, weil das Gel als Molekularsieb wirkt, so dass sich ein größerer Teilchenradius stärker hemmend auf die Wanderungsgeschwindigkeit auswirkt, als nur durch die Viskosität alleine zu erwarten wäre. Durch die unterschiedliche Innenladung und den Teilchenradius bewegen sich die einzelnen Stoffe (Moleküle, DNA, Proteine) unterschiedlich schnell durch das Trägermaterial und erreichen eine Auftrennung ent sprechend ihrer elektrophoretischen Mobilität. Die wichtigsten Detektionsmethoden bei der Gel-Elektrophorese sind Färbemethoden, Fluoreszenzlabel und Radiomarkierung. Die Detektion als Bild erfolgt über einen Film (z. B. bei radioaktiven Markern) oder einen Flachbettscanner (z. B. bei Färbungsmethoden).The main qualitative methods - agarose gel electrophoresis, SDS-PAGE, isoelectric focusing - serve for the separation of DNA and proteins. Two-dimensional electrophoresis (SDS-PAGE and isoelectric focusing) allows highest possible resolution with separation of the analytes by charge and molecular weight. In the gel electrophoresis plays also the relationship between the particle radius and the pore width of the carrier medium serving gel because the gel acts as a molecular sieve, so that a larger particle radius stronger inhibiting effect on the rate of migration than just by the viscosity alone would be expected. Move through the different internal charge and the particle radius the individual substances (molecules, DNA, proteins) at different speeds through the carrier material and achieve a separation according to their electrophoretic Mobility. The most important detection methods in gel electrophoresis are staining, Fluorescence label and radiolabel. The detection as picture takes place over one Film (eg with radioactive markers) or a flatbed scanner (eg in coloring methods).
Um eine optische Aufnahme (Foto) mit anschließender Auswertung zu ermöglichen wird das System mit Hilfe einer Kamera fotografiert und per Bildverarbeitung weiterverarbeitet. Alternativ oder ergänzend zur Kameraaufnahme kann ein unter der Probe befindlicher Flachbettscanner das Ergebnis des Experiments aufnehmen. Dies beansprucht in jedem Fall die Einbeziehung zumindest zweier Geräte mit entsprechenden Leitungen, Auswerteelektronik und ist insbesondere für miniaturisierte Elektrophoresesysteme, speziell für die zweidimensionale Gelelektrophorese mit großem gerätetechnischem Aufwand verbunden. Die Realisierung einer miniaturisierten Elektrophorese ist entsprechend teuer und weit davon entfernt, ein Massenprodukt zu sein.Around to allow an optical image (photo) with subsequent evaluation the system is photographed with the help of a camera and image processing further processed. Alternatively or in addition to the camera recording can a flatbed scanner under the sample is the result of the Record experiments. In any case, this requires inclusion at least two devices with corresponding lines, evaluation and in particular for miniaturized Electrophoresis systems, especially for two-dimensional gel electrophoresis with big technical equipment Effort connected. The realization of a miniaturized electrophoresis is accordingly expensive and far from being a mass-produced product to be.
Aufgabe der vorliegenden Erfindung ist es daher, ein Verfahren und eine Vorrichtung zu schaffen, mit der die Nachteile des Standes der Technik überwunden werden können, insbesondere mit der in einem einfachen, möglichst kostengünstigen Aufbau, die Funktionen in einer Elektrophoresevorrichtung integriert werden, die bislang durch eine Kamera mit entsprechender Optik realisiert wurden.task The present invention is therefore a method and a To provide device with which overcomes the disadvantages of the prior art can be especially with the in a simple, most cost-effective Construction that integrates functions in an electrophoresis device be realized so far by a camera with appropriate optics were.
Lösung der Aufgabe und Gegenstand der Erfindung ist daher eine Vorrichtung zur Durchführung eines Elektrophoreseexperiments, zumindest ein zwei dimensional aufgebrachtes Trägermaterial und die zu detektierenden elektrisch geladenen Teilchen auf einem Substrat umfassend, wobei das Substrat zumindest teilweise zumindest semitransparent ist und auf der anderen Seite des Substrats in dem zumindest semitransparenten Bereich sich zumindest teilweise eine organische Photodetektormatrix befindet. Außerdem ist Lösung der Aufgabe und Ge genstand der Erfindung ein Verfahren zur Detektion elektrophoretisch aufgetrennter Analyten, wobei die optische Information eines Elektrophoreseexperiments durch zumindest einen organischen Photodetektor aufgenommen wird.Solution of Object and subject of the invention is therefore an apparatus to carry out a Electrophoresis experiment, at least a two dimensionally applied support material and the electrically charged particles to be detected on a Substrate, wherein the substrate at least partially at least Semitransparent and on the other side of the substrate in the at least partially a semitransparent area at least partially organic photodetector matrix is located. Besides, solution is the Task and Ge object of the invention, a method for detection electrophoretically separated analytes, wherein the optical information an electrophoresis experiment by at least one organic Photodetector is recorded.
Die Verbindung komplizierter Elektrophoreseexperimente, wie sie beispielsweise schon auf miniaturisierten Chips realisiert werden mit großflächig auftragbaren und bei niedrigen Temperaturen prozessierbaren Photodetektoren ist die bislang einzig bekannte Möglichkeit, Elektrophoresechips tatsächlich massenfertigungstauglich zu gestalten und damit eine fundamentale Analysemethode kostengünstig und massenfertigungstauglich zur Verfügung zu stellen.The Connection of complicated electrophoresis experiments, as for example can already be realized on miniaturized chips with large applicability and processable photodetectors at low temperatures the only known possibility so far Electrophoresis chips actually to make it suitable for mass production and thus a fundamental one Method of analysis inexpensive and to make mass production available.
Nach einer bevorzugten Ausführungsform der Vorrichtung ist die Detektormatrix pixeliert.To a preferred embodiment of Device is the detector matrix pixelated.
Nach einer vorteilhaften Ausführungsform der Vorrichtung ist das Substrat zumindest in Teilbereichen transparent mit einer Lichtdurchlässigkeit von zumindest 10%.To an advantageous embodiment of the Device, the substrate is transparent at least in some areas with a light transmission of at least 10%.
Nach einer vorteilhaften Ausführungsform der Vorrichtung befindet sich auf der gesamten Fläche der einen Seite oder Unterseite des Substrats, die der elektrophoretisch genutzten Fläche auf der anderen Seite oder Oberseite des Substrates entspricht, die Detektormatrix aus organischen Photodetektoren.To an advantageous embodiment of the Device is located on the entire surface of one side or bottom of the substrate that the electrophoretically used surface on the other side or top of the substrate corresponds, the Detector matrix of organic photodetectors.
Nach einer weiteren Ausführungsform des Verfahrens wird die pixelierte Photodetektormatrix für Radiographie eingesetzt.To a further embodiment of the method becomes the pixelated photodetector matrix for radiography used.
Nach einer weiteren Ausführungsform hat die pixelierte Photodetektormatrix einen integrierten optischen Filter für Fluoreszenzmatrizen.To a further embodiment the pixelated photodetector matrix has an integrated optical Filter for Fluoreszenzmatrizen.
In
der einzigen Figur wird beispielhaft der Aufbau eines Photodetektors,
wie er im Sinne der vorliegenden Erfindung eingesetzt wird, erläutert:
Wie
in der Figur gezeigt, bestehen organische Photodioden beispielsweise
aus einem vertikalen Schichtsystem. Als unterste Schicht ist hier
das Substrat
As shown in the figure, organic photodiodes consist, for example, of a vertical layer system. The lowest layer here is the substrate
Die
Unterseite
Des
weiteren ist eine Bottom-Elektrode
Außerdem ist
eine Strom bzw. Spannungsquelle
Durch die Wahl eines hochtransparenten Materials für die absorbierende Schicht und/oder durch die Verdünnung der Licht absorbierenden Schicht, können hochtransparente Dioden hergestellt werden.By the choice of a highly transparent material for the absorbent layer and / or by dilution the light-absorbing layer, can be highly transparent diodes getting produced.
Mit Hilfe der vorliegenden Erfindung können Kosten und Platz für die Kamera und die Optik bislang verwendeter Geräte zur Aufnahme des Bildes eines Elektrophoreseexperimentes reduziert werden. Insbesondere der geometrische Raum für das Elektrophoreseexperiment wird dadurch auch auf ein Minimum reduziert.With Help of the present invention can save costs and space for the camera and the optics of previously used devices to capture the image of a Electrophoresis experiment can be reduced. In particular, the geometric room for the electrophoresis experiment is thereby also reduced to a minimum.
Mit Hilfe der vorliegenden Erfindung sind erstmals komplette Elektrophoresesysteme auf einem Chip von einigen Quadratzentimeter realisierbar. Dabei können diskrete organische Photodetektoren (Fläche von einigen mm2 bis cm2) an bestimmten Stellen des Substrates angebracht werden, oder es wird eine pixelierte Detektormatrix (aktiv- oder passiv Matrix) zur Bildaufnahme implementiert. Dabei sind Pixelgrößen von einigen μm2 bis zu mm2 denkbar.With the aid of the present invention, complete electrophoresis systems can be realized for the first time on a chip of a few square centimeters. In this case, discrete organic photodetectors (area of a few mm 2 to cm 2 ) can be attached to specific locations of the substrate, or a pixelated detector matrix (active or passive matrix) for image acquisition is implemented. In this case, pixel sizes of a few microns 2 up to mm 2 are conceivable.
Die vorliegende Erfindung betrifft ein Verfahren und eine Vorrichtung zur Detektion elektrophoretisch aufgetrennter Analyten, wobei Kosten und Platz für die Kamera und die Optik bislang verwendeter Geräte zur Aufnahme des Bildes eines Elektrophoreseexperimentes durch den großflächigen Einsatz organischer Photodetektoren eingespart werden.The The present invention relates to a method and an apparatus for the detection of electrophoretically separated analytes, whereby costs and room for the camera and the optics of previously used devices to capture the image an electrophoresis experiment by the large-scale use of organic Photodetectors can be saved.
Claims (7)
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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EP3413367A4 (en) * | 2016-02-03 | 2019-01-30 | Nissan Chemical Corporation | Charge transport varnish |
Citations (2)
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---|---|---|---|---|
US20030222223A1 (en) * | 2002-06-03 | 2003-12-04 | Toshihiro Kamei | Solid-state detector and optical system for microchip analyzers |
WO2006063952A1 (en) * | 2004-12-16 | 2006-06-22 | Siemens Aktiengesellschaft | Organic-based electronic component containing pixels |
-
2007
- 2007-08-17 DE DE102007038906A patent/DE102007038906A1/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030222223A1 (en) * | 2002-06-03 | 2003-12-04 | Toshihiro Kamei | Solid-state detector and optical system for microchip analyzers |
WO2006063952A1 (en) * | 2004-12-16 | 2006-06-22 | Siemens Aktiengesellschaft | Organic-based electronic component containing pixels |
Non-Patent Citations (3)
Title |
---|
CASADO-TERRONES,S. et.al: Simple luminescence dete ctors using a light-emitting diode or a Xe lamp, o ptical fiber and charge-coupled device, or photomu ltiplier for determining proteins in capillary ele ctrophoresis: A critical comparison. In: Analytica l Biochemistry 2007, Vol. 365, S. 92-90; SHINAR,Ru th, CHOUDHURY,Bhaskar et al.: Stucturally Integrat ed Organic Light-Emitting Device-Based Sensors for Oxygen, Glucose, Hydrazine, and Anthrax. In: Proc eedings of SPIE, 2004, Vol. 5588, S. 59-69 |
CASADO-TERRONES,S. et.al: Simple luminescence detectors using a light-emitting diode or a Xe lamp, optical fiber and charge- coupled device, or photomultiplier for determining proteins in capillary electrophoresis: A critical comparison. In: Analytical Biochemistry 2007, Vol. 365, S. 92-90 * |
SHINAR,Ruth, CHOUDHURY,Bhaskar et al.: Stucturally Integrated Organic Light-Emitting Device-Based Sensors for Oxygen, Glucose, Hydrazine, and Anthrax. In: Proceedings of SPIE, 2004, Vol. 5588, S. 59-69 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3413367A4 (en) * | 2016-02-03 | 2019-01-30 | Nissan Chemical Corporation | Charge transport varnish |
US11028277B2 (en) | 2016-02-03 | 2021-06-08 | Nissan Chemical Corporation | Charge transport varnish |
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