DD279265A1 - METHOD FOR IN VIVO ROOTING AND SIMULTANEOUS FREELANDADAPTATION OF CHAMOMILLA RECUTITA SPROUTS - Google Patents

Abstract

The object of the invention is to simplify and economize on the cloning process of high yield Chamomilla recutita plants. According to the invention, the sprouts, which have been propagated in vitro, are poked into moist perlite and adapted to the conditions of the greenhouse under initially high, later reduced and fluctuating atmospheric humidity. At the same time rooting takes place, so that after 10 days plants are obtained that can be cultivated further in the greenhouse.

Description

Field of application of the invention

The flower baskets of Matricaria chamomilla (chamomile) are used as a raw material for medicines. Dio invention enables the rationalization of the breeding of new, better camomile varieties, starting from high performance plants. It creates the opportunity to simplify the maintenance breeding of these new varieties.

Characteristic of the known technical solutions

The propagation of high performance plants of Chamomilla recutita, carried out according to patent DD 250949, comprises the steps of planting shoots, propagating shoots, rooting the shoots and adapting the in vitro produced plants to the conditions of the greenhouse. The phases of rooting and adaptation to the constrictions of the greenhouse take a relatively long time and bind cultivation space, labor and chemicals. In various methods of in vitro propagation of plants, it has therefore been attempted to concurrently proceed with the rooting of the in vitro produced shoots and the adaptation phage (see George, EF, Sherrington, PD: Plant Propagation by Tissue Culture, Exegetics Ltd. Eversley, Basingstoke, Hants, England, pp. 370-371, 1984; Evans, D A., Sharp, WR, Ammirato, PV, Yamada, Y .: Handbook of Plant Cell Culture, Macmillan Publishing Co., New York , 211, 1983; Debergh, P.C., Maene, L .: Scientia Hort., 14,335-345 (1981)). For chamomile, however, the application of this method was not obvious because of the small shoot and sheet size and a related low survivability difficulty in the transfer according to patent DD 250949 occurred. Furthermore, there were negative results in this area for other medicinal plants (Digitalis lanata, Rauwolfia serpentina, Foeniculum vulgare). Preliminary experiments on field adaptation with uninjured and rooted Chamomilla recutita sprouts using beakers for air termination and soil / soil / sand mixtures indicated that root regeneration and adaptation are severely affected by infection. The risk of contamination of injured pancreatic tissue and the resulting death of the affected shoots was much higher than in intact Chamomilla reculita plants. The problem is solved according to the invention that rooting and transfer of the shoots in perlite and under film tents success !. Through a high planting density (distance of the shoots to each other 2cm) in the Pikierkästen to form a stable microclimate success rates of almost 100% could be achieved.

Object of the invention

The aim of the invention is the shortening and rationalization of the method for in vitro propagation of chamomile high-performance plants by the simultaneous implementation of rooting and adaptation to the conditions of the greenhouse.

Explanation of the essence of the invention

The object of the invention is to provide Ki'ltivationsbedingungen for in vitro obtained chamomile sprouts, which allow the rooting of these shoots and their adaptation to oie conditions of Gewächsha jses. This shortens the cloning process for high performance crops by the in vitro fertilization phase and saves material, greenhouse area and manpower.

embodiments

Single shoots with a minimum size of 1 crn from the propagation cycle are pikiert on hand moist perlite (distance of the shoots to each other about 2cm). To ensure an initially high and constant ambient humidity, the Pikierkästen be set up with the rungs in film times. The plant material is sprayed periodically with water and starting increasingly aerated for a long time on the second day. The ventilation time depends on the state of the shoot and should be stopped at the latest when the leaves start to wilt. To accelerate the acclimatization process, ventilation is carried out several times daily after about 5 days. Within 10 days, the sprouts have adapted to the environmental conditions of the greenhouse, and the foil tent can be removed. After one month's growth at 20 + 2 ° C and additional exposure, the resulting plantlets are suitable for transfer to soil and further cultivation without precautions. The supply of mineral salts is secured during the adaptation and rooting phase by weekly casting with a 0.1% Wopil ^R nutrient solution.

Claims (5)

1. Rationalization method for in vivo rooting and equilateral outdoor adaptatior. Chamomilla recutita sprouts, characterized in that rooting and adaptation to the environmental conditions of the greenhouse occur simultaneously. 2. The method according to claim 1, characterized in that the propagated in vitro shoots piqued without the addition of growth factors and nutrients in moistened with water substrate and first at high and constant, later at reduced and fluctuating relative humidity (50-70%) 10th Days left. 3. Process according to claims 1 and 2, characterized in that the substrate is inorganic material, preferably perlite ", is used. 4. Process according to claims 1-3, characterized in that the shoots closely, preferably at a distance of 2cm to each other, are pikiert in the pearlite. 5. Process according to claims 1 to 4, characterized in that the adapted to a relative humidity of 50-70% shoots are left for a further 10 days in the Pikierkasten, wherein a root system is developed that the transfer of the resulting plants in soil and their further cultivation in the greenhouse and field allows.