DD217432A1 - DEVICE FOR CLEANING PISTON PIPETTES - Google Patents

Abstract

Apparatus for cleaning Kolbenhubpipettenspitzen that can be used in analytical laboratories, especially in medical with protein, infectious, aggressive or radioactive test materials or solutions. It enables a large number of piston pipette tips to be flushed at the same time, making them reusable multiple times. The device is designed so that pipette tips of different size and number can be cleaned. Via an upper part, the flushing liquid passes through a distribution plate provided with openings and a sealing plate into the attachment openings of the pipette tips. The tips are located in a basic body, in which a segment holder is inserted along with segments, with the pipette tips are in the openings of the segments. Furthermore, seals, adjusting and clamping devices and grip and support elements are present. Fig. 1

Description

Title der.Erfindung

Device for cleaning Kolbenhubpipettenspitzen

Field of application of the invention

The invention relates to a device for cleaning Kolbenhubpipettenspitzen that in analytical laboratories (especially in medical or other with protein-containing or infectious or aggressive or radioactive testing materials and solutions bypassing laboratories) can be used, in which Kolbenhubpipetteri are used, and with a variable Number of tips can be rinsed simultaneously.

Characteristics of the known technical solutions Devices for cleaning pipette tips of reciprocating pipettes have hitherto not become known since the pipette tips were discarded after a single use. DE 1491738, DE-GM 1913734, WP 13O5OO, "nationalization of the medical laboratory" (R. Heckel, ß-I-T Verlag Ernst Giebler, Darmstadt 1976) are methods and devices for cleaning cannulas, Probenbenältern, reagent tubes, pipettes and the like. cited. In particular, a device for automatic cleaning of pipettes is presented in the AS DE 1598454, in which the arranged in a false floor pipettes protrude into a wide, upright standing Spülbehälter · The device is provided with means for supplying and discharging liquid cleaning agents, wherein a substantial Characteristic of this solution is a connected pump and its control. The effort which is required for the cleaning of pipettes, in particular of pipettes with capillaries, is necessarily high. All solutions known hitherto are suitable for the cleaning of piston pipette tips.

suitable, since they are structurally not designed for this purpose and, moreover, the ratio of cost to use is very high, that is, ineffective with regard to the cleaning of tips.

Aim of the invention;

It is the object of the invention to provide a device for cleaning Kolbenhubpipettenspitzen that allows for low konstrukv tive and assembly effort to rinse a variety of pipette tips simultaneously and to achieve a sufficient quality of cleaning, so that the pipette tips can be used multiple »

Explanation of the essence of the invention

The invention is based on the object, starting from the WP A61M / 245127 ·? to construct a device for cleaning piston pipette tips of different size and variable number, in which the pipette tips pass under pressure as flushing liquid tap water, such a quality of cleaning is achieved that the pipette tips can be used repeatedly and Placement of the cleaning device prepares little effort. ,,

According to the invention the object is achieved by the features listed in the characterizing part of the invention claim. The advantages of the invention are to be seen in the fact that the Kolbenhubpipettenspitzen after cleaning can be used again, the device itself is a simple construction, essential to the invention parts of the device are adapted to the analytical operation in laboratories and the placement, or emptying of the device little Time is required.

embodiment

The invention will be explained in more detail below using an exemplary embodiment. In the accompanying drawings: FIG. 1 shows the device according to the invention

Fig. 2 segment holder with segment. ·

Fig. 3 basic body

From Figure 1 it can be seen that the device 1 for cleaning the pipette tips 2 consists of a base body 3> a segment holder 4, the segments 5 and an upper part 6. The segments are provided with openings 7 for receiving the pipette tips 2,

wherein the diameter of the openings 7 is slightly smaller than the largest outer diameter of the pipette spits 2 to be used. On a conveyor plate 8, a sealing plate 9, which consists of conventional material used for sealing, is fastened by connecting elements 10. The distributor plate 8 is in turn connected to the upper part 6 via other releasable connecting elements 11. Sealing plate 9 and distributor plate 8 have appropriate bores 12, 13 · They coincide with the openings 7 in the segments 5, when the segments 5 are inserted into the segment holder 4, this inserted into the base 3 and the top 6 was properly placed. For adjustment serves once a groove 14 in the middle of the base body 3 and a corresponding web 15 in the segment holder 4, see Fig. 2 and 3 · Äußerdem are on the circumference of the upper part 6, an adjusting rod 16 and an associated guide 17 on the base body 3 is present. A handle 18 on the segment holder 4 is mainly used for putting in and taking out the segment holder 4. On the main body 3 more feet 19 are still attached. For the transport of the device 1 and for lifting the upper part 6 are located on this two opposing Griffelernente 20. A supply line 22, a crosspiece 23 and a double olive 24 provide the connection of the device 1 to a water / compressed air network, not shown. On the upper part 6 of the better pressure distribution due to several Schlaucholiven 21 are symmetrically distributed. Upper part 6 and body 3 are firmly connected to each other via a plurality of clamping screws 25. Between upper part 6 and main body 3 are seals 26, between upper part 6, and distributor plate 8, the seals 27. '.''' \

^E ^ 2 shows the view of a segment holder 4, which is fitted with a segment. 5 It consists of the segment supports 41, the handle 18, the Segmentarretierungen 42 and the Justiersteg 15th ..

In Figure 3, the structure of the base body is shown, which consists of a profiled ring 31, a plurality of webs 32 and a middle part 33. The middle part 33 has a notch 14 for adjusting the inserted segment holder 4. On the ring 31, the feet 19 are attached. -

, The device 1 is used for cleaning pipette tips as follows. In the openings 7 of the segments 5, the pipette tips 2 to be cleaned are introduced. The populated segments 5 are

If all segment positions of the segment holder 4 are occupied, this is inserted into the open device 1 in the main body 3, wherein the adjustment of the segment holder 4 in the base body 3 via the notch 14 and the web 15 takes place. s

Subsequently, the upper part 6 is placed. When placing the adjusting rod 16 must be inserted into the guide 17 so that the openings 7 of the segments 5 with the holes 12, 13 match. By means of the clamping screws 26 upper part 6 and body 3 are firmly clamped. After opening a stopcock, not shown, the inflow of the rinsing agent via the supply line 22 into the device 1. About the holes 12, 13 in distribution plate 8 and sealing plate 9 enters the Spülfliissigkeit in the pipette tips 2. Flows through this under increased pressure and then passes to a not shown collecting or drainage device. After a reasonable rinsing time, the ..- supply of detergent is interrupted. This can be followed by a blow-out of the pipette tips 2 by adding compressed air to the device 1 via the attachment 22 via another stopcock (also not shown). After the closing of the compressed air shut-off valve, the device 1 is opened by loosening the clamping screws 26 and removing the upper part 6. The segment holder 4 is detected and removed on the handle 18. Subsequently, the segments 5 can be removed from the segment holder 4. The cleaned pipette tips 2 can now be removed from the segments 5 or, if necessary, can be supplied together with them for further treatments (for example sterilization).

For the invention, it is irrelevant whether a round or an angular shape for the cleaning device 1 is selected. On the other hand, the following aspects are essential: According to the size of the pipette tips 2 used, the openings 7 of the segments 5 are also different. It is advantageous to provide one segment 5 completely with openings 7 for one pipette tip size and another segment 5 with openings 7 for a different pipette tip size. Furthermore, either covers for unequipped segments 5 or slightly thicker segments 5 without openings 7, so-called "dummy segments ¹¹ , are provided, so that the cleaning liquid does not escape from the openings 12, 13 of the distributor or sealing plate 8 at these locations. 9 can escape, which otherwise

a pressure loss would occur. .

As a result, cleaning is also effectively possible if not all openings 7 of the segments 5 with pipette tips 2 or the segment holder 4 are not completely covered with segments 5. Furthermore, the number of openings 7 per segment 5 is selected so that the corresponding Operation in laboratories, a rinsing process can be done relatively quickly, so that an intermediate storage in aqueous or similar Solutions are eliminated. Certain applications may prove advantageous when the segments 5 and segment holders 4 and handle 18 form a structural unit (e.g., circular plate).

Claims (3)

Erf indungsan saying Spooling liquid consisting of a main body, an upper part together with distribution and sealing plate and seals and connecting elements, characterized in that on the upper part (6) a distributor plate (8), a sealing plate (9), a plurality of connections (21) for a supply line (22) and gripping elements (20) and Just guiding devices (16) are fixed, 'that the base body (3) with an adjusting device (14) for a segment holder (4), a second adjusting device (17) for the upper part (6), support elements (19) and devices (31> 32, 33) for receiving the segment holder (4) is provided such that the segment holder (4) consists of receiving (41) and locking devices (42) for segments (5 ) and another handle 'element (18), that the segments (5) have openings (7) for receiving the pipette tips (2), wherein the diameter of the opening (6)' an outer diameter of the pipettes (2) corresponds to that seals (26, 27) between the base body (3) and upper part (6) and between the upper part (6) and distributor plate (8) are present, that the number of, openings (12, 13) in distribution plate (8), the sealing plate (9) is equal to the number of openings (7) in the segment holder (5) fully equipped segment holder (4) and the openings (12, 13) in the plates (8, 9) in the closed device (1 ) are located above the openings (7) of the segments (5) that on unequipped segments (5) covers are present, that the multi-part supply line (22) for the rinsing liquid and scavenging air over a plurality of symmetrically on the upper part (6) arranged connecting elements (21) is connected to the upper part (6) and that for the assembly of upper part (6) and base body (3) connecting elements (25) are present » 2. Device according to item 1, characterized ^ ^ that at the location of a not equipped with pipette tips (2) segment (5) in the segment holder (4) is a blind segment during the flushing process. 3 * Device according to 'Item 1, characterized in that an unsegmented plate with openings (7) for receiving the pipette tips is present, the number and arrangement of the openings (7) with those of the openings (12) in the distributor plate (8). matches.