DD201732A5 - DEVICE FOR ULTRAMIC ROSCOPIC OBSERVATION - Google Patents

Abstract

The device is applicable to free microelectrophoresis apparatus for the visual observation of small particles which are invisible when observed in a transmitted light microscope. The aim is to increase the scope and accuracy of measurement. The object is to allow the thermostating of a vertically arranged rectangular electrophoretic chamber by the possibility of investigating electrophoretic mobilities of small colloidal and biol. Particles with dimensions of 0.5 to 0.0005 to create. The device according to the invention contains a light source, an optical system of tube, lenses, deflection element and sleeves, a rectangular electrophoretic chamber and a microscope. The rectangular electrophoretic chamber 2 is arranged vertically, and the microscope is mounted opposite its wide side wall. The opt. Main axis 7 of the part of the optical system containing tube 4, sleeve 5 and lenses 3 is opposite to the opt. Main axis 11 of the microscope objective parallel and spatially offset. Areas of application are biology, medicine and agriculture.

Description

Device for ultramicroscopic observation Field of application of the invention

The invention relates to a device for ultramicroscopic observation, which can be used on the apparatus for free microelectrophoresis with a rectangular electrophoretic chamber, for the visual observation of small particles, which are invisible when observed in a transmitted light microscope · Applications are biology, medicine and agriculture ·

Characteristic of the known technical solutions

A device for free microelectrophoresis (Patent Hr 4 046 667) is already known, which comprises a light source, a rectangular electrophoretic chamber, sleeves, a microscope and an optical system _f consisting of a tube, lenses and a deflection element.

The disadvantage of this device is that the electrophoretic chamber is not thermostated, which leads to the formation of temperature gradients and corresponding to the liquid skonvek tion in the chamber during the working phase '' 7 '. · ". ' : '' '.7-' '.:: /',. ' . ":" ν '·'; - - -. - '',. '. "

Another Hachteil is that the electrophoretic chamber is arranged horizontally. When measuring particles heavier than water, they settle and leave the horizontal stationary planes.

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Object of the invention

The object of the invention is to increase the scope of the device for ultramicroscopic observation and their accuracy of measurement.

Explanation of the essence of the invention

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The object of the invention is to provide a device for ultramicroscopic observation, which allows the thermostatting of a vertically arranged rectangular electrophoretic chamber and the possibility of investigating electrophoretic mobilities of small colloidal and biological particles with dimensions of 0.5 / um to 0.0005 / um offers.

This object is achieved in that in the device for ultramicroscopic observation, which contains a light source, an optical system of a tube, lenses, a deflector and sleeves, a rectangular electrophoretic chamber and a microscope, the rectangular electrophoretic chamber is arranged vertically and the microscope is mounted opposite its wide side wall ". Here is the main optical axis, the part of the op ti" *. see system containing the tube, the sleeves and lenses, parallel and spatially offset from the main optical axis of the microscope objective.

The advantages of the device according to the invention for ultramicroscopic observation are that the sensitivity of the apparatus is increased, which makes it possible to examine objects such as two-layered lipids

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Liposomes (model artificial membranes), submitochondrial particles and the like. a · guaranteed with dimensions of 0.5 / .mu.m to 0.005 / .mu.m and the measurement accuracy is improved.

Ausführungsbei game

The invention will be explained in more detail below using an exemplary embodiment. In the attached drawings show?

Pig · 1: a basic scheme of 3inrichtungj

Pig. 2ϊ is a graphic representation of the velocity distribution of the submitochondrial particles in a rectangular electrophoretic chamber.

The light source 1 (Pig _e 1), which is a fed with mains voltage 220 V and at a distance of 40 to 50 cm from the rectangular electrophoretic chamber 2 mounted mercury lamp HBO-200, in front of the lens 3 and the tube 4 with a Diameter of 3 cm arranged, of which a part is housed in the sleeve 5, which can be moved and translation around tube 4 with lens 3 and deflecting mirror 6, which is inclined at 45 ° relative to the optical axis 7, can be rotated. Symmetrically with the deflected in the deflecting 6 deflected optical axis 7 is rigid and vertical in a thermostat 8, mounted between its glass window 9 »the rectangular electrophoretic chamber 2, whose narrow side wall (by 0.1 cm) is perpendicular to the optical axis 7, while their wide side wall is parallel to the glass window 9, opposite which the microscope 10 is arranged. The main optical axis

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of the microscope objective is perpendicular to the broad side wall of the rectangular electrophoretic chamber 2. Opposite the narrow side wall of the rectangular electrophoretic chamber 2, the sleeve 12 with the lenses 3 is arranged »

Pig · 2 shows, between the two coordinates - chamber relative depth and electrophoretic mobility - the distribution of velocity at intervals of measurement inaccuracies obtained from rat liver mitochondria during aging in the electrophoretic chamber.

The terms used in Fig. 2 are as follows:

"-" fresh (functional) particles; ο mitochondrial particles exposed to -4 ⁰ C for 24 hours;

Mitochondrial particles exposed to +4 ⁰ C for 24 hours;

Mitochondrienteilchen that were exposed to 48 hours at a temperature of -4 ⁰ C.

The light beam generated by the light source 1 passes through tube 4, sleeve 5 and the lenses 3 and is deflected by the deflecting mirror 6 by 90 ° »The deflected bundle goes through sleeve 12, with the lenses 3, in the thermostat 8 and falls on the narrow Side wall of the rectangular electrophoretic chamber 2 The 90 ° scattered light from the particles is observed with the microscope 10 through the glass window 9. Through the sleeve 4 moving on the sleeve 5, the light beam is directed precisely into the rectangular electrophoretic chamber 2 while passing through lenses 3 its focusing takes place ·

Claims (1)

23395 I 7 -.5.- 20.1.82 59 906 16 Erfindun ^ sanspruch Apparatus for ultramicroscopic observation, comprising a light source, an optical system of tube, lenses, deflector and sleeves, a rectangular electrophoretic chamber and a microscope, characterized in that the rectangular electrophoretic chamber (2) is arranged vertically and opposite to it the main optical axis (7) of the part of the optical system containing the tube (4), sleeve (5) and lenses (3) is opposite the main optical axis (11) of the Microscope lens is parallel and spatially offset. For this 2 pages drawings