DD156199A1 - ARRANGEMENT FOR PRODUCING NATIVE TISSUE CUTS - Google Patents

Abstract

The arrangement for producing native tissue sections is suitable for producing sections in medicine and biology from non-fixed or / or fixed biological tissues in order to make statements of a morphological nature. The aim of the invention is to produce faster cuts of native tissue using a conventional microtome. The object of the invention is to increase the oscillation frequency by eliminating electromechanical oscillators. According to the invention, this is achieved by generating a high-frequency oscillation by means of an electroacoustic transducer and raising it by means of a radiation horn, thereby placing a knife in the desired motion.

Description

Title of the invention

Arrangement for producing native tissue sections

Field of application of the invention

The invention relates to an arrangement for producing sections of unfixed or affixed biological tissues for the purpose of depicting or preparing the depiction of morphological structures and the processes and changes that can be derived therefrom in the tissue, in particular of nervous tissue.

The same arrangement is used for easier or defined production of cuts and slices for the purpose of electrophysiological derivatives.

Characteristic of the known technical solutions

Progress in the field of histochemical and electrophysiological techniques is characterized by the production of native tissue sections. Only then is it possible to apply special chemical methods to obtain new insights.

To realize this requirement, a single device known as "Vibratom" manufactured by "Oxfort Laboratories", 107 № Bayheore Blvd, San Mateo, Calif, is known internationally.

The function of this device is based ^ ^ that a suitable knife is placed in a tempered with ice pieces buffer solution in 50 (60) 11 ^ vibrations and is driven at a feed rate of a few millimeters per minute through the tissue. This feed rate and thus the cutting time depends on

the deflection (oscillation amplitude) of the knife,

- the frequency (oscillations per second, here 50 or 60 Hz) dG3 knife,

- to be cut to the minimum object size and · »the cutting roughness of the knife»

With the parameters shown (Fig. 1), the limits of the device are fixed, which consist in that

- The tissue is continuously compressed and pulled over a longer period due to the circular or elliptical path of the knife per oscillation

- and only a few cuts of a sample can be won «

The extremely low feed rate must be realized by means of complex and low-vibration transmissions and gear ratios. "An increase in the oscillation frequency, a required reduction in the oscillation amplitude of the knife as well as a shortening of the cutting times are possible only insignificantly with the electromechanical oscillator used.

Object of the invention

The aim of the invention is to produce sections of IT tissue in a much simpler, cheaper and faster manner using a conventional microtome.

Explanation of the invention of the invention

The invention is based on the objective of producing cuts of native tissue "in a shorter time by increasing the oscillation frequency and waiving electromeban oscillators.

The object is according to the invention dadarcb. solved that ßin suitable

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netes knife is offset by a transmitter in high-frequency oscillations. For this purpose, a multi-part arrangement is necessary, which consists of an operated by oineiri control unit encoder, which is coupled via a held by retaining ring radiation horn.

A control device as a generator of variable amplitude electrical oscillations excites a transmitter in the form of an electroacoustic transducer whose oscillation amplitude is increased by the radiation horn following it. The knife holder with knife performs the same vibration mode as the tip of the radiant horn.

This assembly is mounted on a microtome instead of an embedded tissue knife and is movable as usual.

The structure of the arrangement according to the invention is uncomplicated in terms of its application and completely adapted to the practical requirements in medicine and biology (Fig. 2).

Ausführungsbei game

The invention will be explained in more detail below with reference to an exemplary embodiment:

In Fig. 3, the front view of the arrangement for producing native tissue sections is shown *. On a slide microtome main body (1) is located in place of the microtome knife, the radiation horn (3), via the retaining ring (4 ·) with the carriage (2) rigid connected is. The piezoceramic oscillator (5) as an encoder is excited by a control unit (6) with a variable output voltage to oscillations of (3) / amplitude at a frequency of 30 kHz. The cap (7) serves to hold the vibrator (5) and secures against accidental contact. With the calculated geometrical dimensions of the radiation horn (3) made of aluminum, a vibration amplitude of 0., 30 / .mu.m is given at its tip in a horizontal direction. The knife holder (8) consists of ceramic and is exactly in its gravity line via the connection (9 ) made of hard stainless steel firmly connected to the tip of the radiant horn (3) »The knife holder (8) carries the razor blade used as a knife (10), which is fastened by the noble-metal clamp (11).

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is retarded. When mounted diametrically, the balancing mass (12) prevents undesired vibration modes from occurring. The tissue to be cut (13) is "on a stud (14) of wood or other physiologically harmless material bonded with ¹¹ Geweboklebstoff LKxAMENT FBlOMED and ^Rt is the Peltier of the batteries (15) tempered Bifferlösung (16). The adjustable current and voltage values for the Peltier batteries (15) provided by the power supply unit (17) allow lowering the temperature of the Bifferlösung (16) in the copper heat (18) to 0 ... 4- ⁰ C, Pur sufficient temperature stability of Biff release (16) provides thermal shielding (19) throughout the entire operating period. For ease of visual inspection, tissue (13) is passed through a light guide (23) held by clamp (24) through a light source (21). with a fitting (22) laterally illuminated with almost "shadowless" light. Below the block (14) is a magnet (25) which is attracted by a steel plate (26) mounted outside the copper trough (18). This ensures a stationary position of the fabric (13). The radiation horn (3) is rotatably mounted in HaI-tering (4), whereby the cutting angle between knife (10) and tissue (1J) can be selected arbitrarily and read on the marking (28). The function of the Peltier batteries (15) requires a cooling water inflow and outflow (27).

During the cutting process, the arrangement consisting of 2 * .5? '<>' .I2 is manually moved in the frontal direction on the carriage microtubule body (1). The knife (10) penetrates through the tissue (13) in a "sawing" way and vice versa. With the microtome own linkage (20), the desired slice thickness can be selected. The sections thus produced are carried by the Pafferlösung (16) and are ready for further use.

Claims (2)

- ⁵ - 11 / bi 1. Arrangement яиг production паѣіѵѳг tissue sections ила Gev / еЪе sections by means of a Mikrotomgrundkörpars (1) with slide (2) and knife holder (8) with a knife (10) characterized in that a knife is placed in high-frequency oscillations ·. 2. Arrangement according to Hinkt 1, characterized in that the high-frequency oscillation by an electro-acoustic transducer ^ (5) and generated by a radiation shorn is increased. For this purpose 1 Sgü © drawings