CZ25594U1 - Tool for withdrawal of oocytes and ovarial follicle liquid in cows by making use of standard replaceable needles s - Google Patents

Description

Technical field

The invention relates to an ultrasonic probe holder in conjunction with a device that allows for the collection of oocytes and follicular fluid. Standard interchangeable injection needles are used.

Background Art

Trans vaginal ultrasonographic ovarian puncture in cows is routinely used to collect oocytes (ovum pick-up method, OPU) for in vitro embryo production and recently also to collect follicular fluid for analysis. Equipment for this activity is produced by many companies on a similar principle.

The workplace of ruminant diseases clinic VFU Brno uses the devices of the company Aloka. A special rod-shaped plastic ultrasonic probe holder (sonography Aloka SSD-500, probe Aloka UST 974-5) ensures that the probe is placed in the appropriate place in the vaginal vault and the ovary is pressed manually by the rectum against the probe. All ovarian structures are visualized on the sonograph screen. There is only a thin vaginal wall between the active probe surface and the ovary, through which the aspiration needle can be easily and without sequelae. In the upper part of the probe holder there is a metal needle conductor in which the operator moves the disposable aspiration needle (V-OPAA-1760, Cook, diameter 1.3 mm, length 60 cm), connected to a vacuum aspiration system. The tip of the needle penetrating the follicle is visible on the sonograph screen, the operator, depending on the situation on the screen, controls the holder and needle with one hand, the ovary with the other hand and the vacuum aspiration pump switch with the other.

The essence of the technical solution

It is a technical tool that modifies the OPU system described above. They differ by replacing the aspiration needle conductor and the aspiration needle itself with a special device built into the top of the rod holder, which allows the use of standard interchangeable needles.

The proposed mechanism consists of a cylindrical body, at which conical needles (any Luer injection needles) are inserted at the conical end. Needles can be easily replaced after each use, ensuring proper sharpness and sterility. Needle diameter and length selection can be controlled by the size and availability of the aspirated object. Also, the cost of standard needles is significantly lower compared to special puncture needles in existing tool types. The cylindrical needle body moves in a metal conductor located at the top of the specially adapted plastic probe holder, similar to the original OPU system, but varies in size and shape.

Brief Description of the Drawings Figure 1 shows the probe holder.

Figure 2 shows oocyte and ovarian follicular fluid collection devices.

Oocyte and follicular fluid collection devices consist of:

1) Ultrasonic probe

2) Probe holder

3) Guide tube

4) Needle wire

5) Aspiration needle.

-1 CZ 25594 Ul

Description of individual parts:

1) Ultrasonic Probe i

A standard ultrasonic probe can be used for application. The ultrasound probe is placed in the holder at an angle so that a good aspiration needle image is possible during application.

2) Probe holder 2

It is made of two parts that are bolted together. Probe I and guide tube 3 are attached to probe holder 2. For easy maintenance, the holder can be disassembled. The external dimensions and length of the probe holder make it easy to insert into the vagina of the examined animal.

3) Guide tube 3

The guide tube is made of a metal tube. It is mounted in the probe holder and serves to insert the needle 4 conductor and its free movement.

4) Needle wire 4

There is an internal movable part that the operator moves with his or her own aspiration along with the needle 5. The needle guide 4 includes a channel in the inner part that opens at the apex of the cone end of the conductor. An aspiration needle 5 is attached to this cone. At the rear end, the needle guide 4 is provided with a Luer 6 terminal for attaching the aspiration tube. The individual conductor parts of the needle 4 are made of rigidly bonded components.

5) Aspiration needle 5

Any Luer injection needle of appropriate length and diameter (typically 1.2 * 40mm, 17G) is used.

An example of a technical solution

The oocyte collection device and follicular fluid using interchangeable needles is a rod-shaped 550 mm long, 25 mm thick and 25 to 60 mm wide. The main support that determines the shape of the entire tool is the probe holder 2. It is made of two parts that are bolted together. Tunnels are provided inside the holder 2 for receiving the ultrasonic probe 1 and the guide tube 3. The ultrasonic probe 1 is located at an extended portion of the lower tunnel at the front of the holder 2 at an angle such that a good image of the aspiration needle is possible during application. The active surface of the probe 1, which is visible from the outside in the front of the tool, passes smoothly into the holder 2. The guide tube 3 is made of a metal tube. It is mounted firmly in the upper tunnel of the probe holder 2 and serves to introduce the needle guide 4 and its free movement. Only the ends of the guide tube 3 can be seen from the outside. The front end of the guide tube 3 has a narrow outlet opening which serves to pass the aspiration needle 5, the rear end and the entire body of the guide tube 3 is wide to allow the needle guide 4 to be inserted and released. the needle 4 is an internal movable portion that the operator moves with the needle 5 during the actual aspiration. The needle guide 4 includes a channel in the inner portion that opens at the apex of the cone end of the conductor. Extracted follicular fluid flows through this channel during aspiration. An aspiration needle 5 is attached to this cone. Any Luer injection needle of appropriate length and diameter (typically 1.2 x 40 mm, 17G) can be used. At the rear end, the needle guide 4 is provided with a Luer 6 terminal for attaching the aspiration tubing.

Functions of oocyte collection devices and follicular fluid using interchangeable needles:

The probe holder 2 is inserted one hand deep into the animal's vagina so that the vaginal wall is taut. The active area of the sonographic probe 1 touches the vaginal vault where the ovary is located behind the vaginal wall in the abdominal cavity, which is held by the surgeon using the fingers of the other hand. The surgeon, using the sonographic probe 1, searches for the target ovary (follicle, cyst). An aspiration needle 5 is firmly attached to the front cone end of the needle guide, and an aspiration tube is attached to the rear end. The needle guide 4 is inserted into the guide tube 3 so that the aspiration needle 5 does not protrude from the probe holder 2. By moving the needle guide, the needle is inserted into the follicle, the vacuum in the aspiration system draws the follicle content into the collection container. After the end of aspiration is

-2C 25594 Ul The needle of the needle 4 is pulled into the probe holder 2 and carefully removed from the animal's sheath. The surgeon makes sure that the removed instrument is complete and undamaged by visual inspection.

Claims (1)

PROTECTION REQUIREMENTS Oocyte and follicular fluid collection device, characterized in that the guide tube (3) is made of a metal tube, is fixed firmly in the upper tunnel of the probe holder (2), the guide tube (3) having a narrow outlet opening which serves for the passage of an aspiration needle (5), while the rear end and the entire body of the guide tube (3) are wide to allow the introduction and free movement of the needle guide (4) to move along with the needle (5); which includes in the inner part of the ducts used for aspiration of follicular fluid during aspiration, and which opens at the apex of the conical end of the front part, where the aspiration needle (5) is inserted, while at the rear end (6) for attaching the aspiration tube.