CZ24895A3 - Testing strip for furnishing proof of substances secreted by urine - Google Patents

Abstract

Test strips for detecting electrolytes in urine contain a dye coupler or colour indicator and a buffer in volume-defined detection zones of the test strip. Detection zones for Mg ions are pref. impregnated with xylidyl blue and Good buffer (pH 11-12); those for Ca ions are impregnated with o-cresolphthalein and Good buffer (pH 10-11); those for phosphate are impregnated with ammonium molybdate and buffer (pH 1-3); those for Fe<2+> are impregnated with "Ferro Zine" and Good buffer (pH 4-6); those for K ions are impregnated with Kryptofix 222 and Good buffer (pH 8-10); and those for Na and Cl ions are impregnated with Mg uranyl acetate and buffer (pH 5-7).

Description

Technical field

The invention relates to a test strip for the detection of substances secreted urine ^muffler that the proof contains electrolyte volume defined conclusive box impregnated cloth to induce a color reaction or color indicator and buffer.

BACKGROUND OF THE INVENTION

In normal management of body products, electrolyte concentrations must be within a predetermined range of values. Serum concentrations refer to extracellular spaces because only the values of hodnoty in extracellular spaces are measurable without major difficulty. Deviations from these values condition disease states of different types and severity. Measuring electrolytes in serum is quite expensive and hardly applicable in daily practice.

The concentration of electrolyte in the urine makes it possible to infer values in serum. The kidney regulates the secretion of substances to maintain serum equilibrium values. In the case of major disorders and conditions, maintaining serum equilibrium values is no longer possible, so variations in concentration in the urine are not possible.

Test papers for determining, for example, sugar and protein are known. Measurement of electrolytes in spontaneous urine is not possible with current measuring papers.

It is further known from the Czechoslovak patent to provide a mechanical test strip for analytical purposes, which consists of a handle, at the lower end of which a carrier saturated with an unspecified reagent is attached. The mechanical structure of this

ι ι

ΐ τ 2 carriers.

Finally, in Czechoslovak Author's Certificate no. 216,388 is $ poṣaṇa method test strip not, determination of calcium in ^L and bio logical material which is based on using a chromogen monoetanólaminového buffer containing kresolftalexon 8hydroxichinolin and dissolved in hydrochloric acid.

It is an object of the present invention to provide a test strip for determining electrolyte concentrations in spontaneous urine. .

SUMMARY OF THE INVENTION

This problem is solved and the deficiencies of similar known test strips are remedied to a remarkable extent by a test strip for the detection of urinary excretory substances which contains electrodeposites containing volume-defined detection fields impregnated with color reaction substances or color indicators and buffer according to the invention. in that the test strip contains a pH indicator (such as methyl red / bromothymol blue 1: 1, always 5 to 50 mmol / l of the impregnation solution) in a standard conclusive field for a pH> 5 to 7 and a creatinine indicator (as an alkaline acid solution) Picrous having a pH> 12, 500 to 5000 phol / l of the impregnation solution, with a creatinine to picrate ratio of 20 to 50 and a urinary creatinine reference interval of 10 to 30 mmol / l),

The solution according to the invention differs from the prior art in that it allows the rapid and sufficiently accurate determination of electrolytes in spontaneous urine. The positive area is defined by color matching fields, as is the case with the test papers of this kind. Therefore, the test strip is best suited for rapid test in medical analytics.

It is preferred that the test strip contains Good buffer in addition to the above indicators.

Another preferred embodiment is that the test strip comprises a standard detection field with xylidyl blue as an indicator of magnesium cations (molar ratio mmo / 1 Mg ^ / xylidyl blue to recognize increased excretion 0.1 to 1, xylidyl blue concentration 0.1 to 1 a buffer having a pH of 11 to 12, for example, a buffer of Good, a reference interval of magnesium and magnesium cations in the urine of 2 to 6 mol / l, an increased excretion of> 2 mmol / l in the urine). ..... '

Another further preferred embodiment is that the test strip comprises a standard detection field with xylidyl blue as an indicator of magnesium cations (molar, mmol / l Mg ^{s +} / xylidyl blue ratio to recognize reduced excretion 1 to 2, xylidyl blue concentration 1 to 10 mmol Buffer (pH 11 to 12, for example, buffer Good, reference interval, magnesium cations in urine 2 to 6 mol / l, decreased, excretion <1 mmol / l in urine).

. Another preferred embodiment is that the test strip comprises a standard detection field with an o-cresolphthalein complexone as an indicator of calcium cations (o-cresolphthalein concentration of 100 to 1000 phol / l of the impregnation solution, Ca ^{i +} / cresolphthalein 10 to 100 ratio, a pH of 10 to 11, such as, for example, buffer Good, a reference interval of calcium cations in the urine of 1 to 9 mmol / l to recognize increased excretion of calcium cations> 10 mmol / l in urine).

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Still further another preferred 'embodiments' ^_ s'pbčívá'vtom ..... that -zkušební-strip includes a standard box conclusive with ammonium molybdate r ~ / vanadium as an indicator for monohydrogenfosfátové anions (molar ratio in mmol / 1 ² HP0 \ / molybdate to recognize increased excretion 1 to 2, molybdate concentration 10 to 50 mmol / l impregnation solution, pH buffer 1 to 3, reference interval i in urine 10 to 40 mmol / l, increased excretion> 30 mmol / l, in urine ).

with

It is also advantageous if the test strip contains a standard detection field with a Ferro Zine as an iron ( ^III ) cation indicator (Fe ^{I +} / Ferro Zine molar ratio / 1 to recognize increased excretion 0.1 to 0.01, Ferro Zine concentration 50 to 500) 1 of a loading solution, a pH 4 to 6 buffer, for example

- Buffer Good, reference, urine interval of 5 to 15 fps, increased excretion> 45 fps in urine). r.

.. · /

Λ

Another possible and advantageous variant is that the test strip comprises a standard detection field with a chromophore associated with Kryptofix 222 as an indicator for the potassium cation (molar ratio in mmol / 1 K * / Kryotofix 222 to recognize increased exclusion>

^J 30 mmol ~ '/ ~ l; -Oyl- to ^- 1 ~ -koncentrace-K-ryptof-2-2-2-1- ixu-to-10 ~ -mmol / lnapouštěcího solution, buffer pH 8-10 , for example, a buffer

Good, urinary potassium cation reference interval 20 to 80 mmol / l, increased urinary excretion> 30 mmol / l).

Finally, it is preferred that the test strip comprises a standard detection field with magnesium uranyl acetate and bromothymol blue as an indicator for the electrolyte pair Na * / ï (molar ratio in mmol / l Na ⁺ / Cl and magnesium uranyl acetate to recognize increased elimination> 40 mmol / l 2 to 20 , concentration of magnesium uranyl acetate 10 to 100 mmo / l of the impregnating solution, buffer

- a pH of 5 to .7. urinary reference interval Na * / Cl in urine 20 to 200 mmol / l, increased urinary excretion> 40 mmol / l).

Thus, the test strip contains, for one detection field on the backing sheet, an impregnable stock such as reagent paper. This stock is impregnated with a mapping solution of the desired type and is sealed with a urine-permeable or water-soluble coating. One test strip may be provided with any number of conjugate fields, corresponding to the number and concentrations of electrolytes to be detected.

Particularly stable results are obtained when the buffer is a buffer

Good ~

To standardize and assess the applicability of the urine test, the test strip is characterized by a pH indicator (such as a 1: 1 methyl red / bromothymol blue system for every 5 to 50 mmol / l impregnation solution) in a standard evidence field for a pH range of 5-7.

DETAILED DESCRIPTION OF THE INVENTION.

The invention is further illustrated by the following non-limiting examples.

Example 1 .....

The creatinine indicator (as alkaline picric acid pH> 12 '500-5000 phol / l impregnation solution, with a creatinine / picrate ratio of 20 to 50, a reference creatinine urine interval of 10 to 30 mmol / l) in a standard conclusive box makes it possible to assess sample usage spontaneous urine. Immediate diagnostic use of the urine specimen is only permitted for creatinine concentrations of 10 to 30 mmol mmol · / l: otherwise, it is necessary to compare to a 24-hour urine collection.

Example 2

Excess of magnesium can be detected by a detection field with xylidyl blue as an indicator for magnesium cations (molar ratio mmo / 1 Mg ² * / xylidyl blue to recognize increased excretion 0.1 to 1, xylidyl blue concentration 0.1 to 1 mmol / l impregnation solution a pH 11 to 12 buffer such as Good buffer, a reference interval of magnesium cations in urine of 2 to 6 mol / l, increased excretion> 2 mmol / l in urine). Hypermagnesaemia requires further therapy.

Example 3.

Similarly, hypomagnesaemia is recognized by the evidence field with xylidyl blue as an indicator of magnesium cations (mmol / l Mg ² * / xylidyl blue molar ratio to recognize reduced excretion 1 to 2, xylidyl blue concentration of 1 to 10 mmol / l of saline solution, a buffer having a pH of 11 to 12, for example, Good buffer, a reference interval of magnesium cations in urine_2 to 6 mol / l ₍ reduced excretion <1 mmol / l in urine). Hypomagnesemia requires parenteral therapy.

Example 4

Hypercalcemia is recognized by an o-cresolphthalein complexone indicator field as an indicator of calcium cations (o-cresolphthalein concentration 100-1000 phol / l impregnation, Ca ² * / cresolphthalein 10-100 ratio, pH 10-11 buffer, for example buffer Good, urinary calcium cation reference interval of 1 to 9 mmol / l to recognize increased calcium cation excretion> 10 mmol / l in urine). Hypercalcaemia requires further therapy.

*

Example 5

Excretion of phosphate is able supportable shelf with ammonium molybdate / vanadate as indicator monohydrogenfosfátové anions (molar ratio in mmol / 1 HP0 ² \ / molybdate to discern elevated exclusion 1-2, the concentration of molybdate 10-50 mmoles / 1 of impregnation solution, a buffer value ' ρΗ 1 to 3, urinary reference interval 10 40 40 mmol / l, increased excretion> 30 mmol / l in urine). A phosphorus deficiency exists when the phosphate concentration value is less than the said value.

Example 6

Iron excretion can be demonstrated with a Ferro Zine field as an indicator of ferrous cations (Fe ²⁺ / Ferro Zine molar ratio per mole / 1 to recognize an increased exclusion of 0.1 to 0.01, Ferro Zine concentration of 50 to 500 mole / l of impregnation solution, a buffer having a pH of 4 to 6, for example, Good buffer, a reference interval in the urine of 5 to 15 fpm, increased excretion> 45 fpm in the urine). Low and increased excretion requires therapy.

Example 7

Proof of potassium cations is possible in a conjugate field with a chromophore associated with Kryptofix 222 as an indicator for potassium cation (molar ratio in mmol / 1 K * / Kryotofix 222 to recognize increased excretion> 30 mmol / l, 0.1 to 1, Kryptofix concentration 200 to 10 mmol / l of impregnation solution, pH 8 to 10 buffer, for example, Good buffer, reference urinary potassium cation interval of 20 to 80 mmol / l, increased excretion. mmol / l in urine). Hypocalcaemia requires therapy.

Example 8

Since the Na * / Cl * electrolytic pair is generally strongly correlated, a conclusive field with magnesium uranyl acetate and bromothymol blue is used as an indicator for the Na * / ï electrolyte pair (molar ratio in mmol / l Na * / Cl 'and magnesium uranyl acetate to recognize increased excretion) > 40 mmol / l 2 to 20, concentration of magnesium uranyl acetate 10 to 100 mmol / l of impregnation solution, pH buffer 5 to 7, reference interval Na * / Cl in urine 20 to 200 mmol / l, increased excretion> 40 mmol / l in power).

Industrial applicability. .

A test strip to quickly determine the insufficient or excess concentration of various electrolytes in the urine to determine if therapeutic measures need to be initiated.

Claims (7)

PATENT CLAIMS Test strip for the detection of urinary excretory substances, which contains electrodytes for the detection of electrolytes, defined by volume-defined detection zones, impregnated with color-inducing substances, optionally with color indicators and a buffer, characterized by:. that it contains a pH indicator (such as ----- methyl red / blue in combination bromthy .mu.mol L .__: 1 ._ yždy _s_až 50_ mmol / 1 ^{&quot; i _ hápóůštěcíhb'roztokuj;} In a standard conclusive field for a pH of 5-7 and a creatinine indicator (as an alkaline picric acid solution of pH> 12, 500-5000 phol / l of the impregnation solution, with a creatinine to picrate ratio of 20- 50 and a urinary creatinine reference interval of 10 to 30 mmol / l). Test strip according to claim 1, characterized in that it contains Good buffer in addition to said indicators. Test strip according to claim 1 or 2, characterized in that it contains a standard detection field with xylidyl blue as an indicator of magnesium cations (molar ratio mmó / 1 Mg ² * / xylidyl blue to recognize increased excretion of 0.1 to 1) xylidyl blue concentration 0.1 to 1 mmol / l of impregnating solution, pH II to Γ2 buffer, eg Good buffer, magnesium urinary cation reference interval of 2 to 6 mol / l, increased excretion> 2 mmol / l in urine) . Test strip according to one of claims 1 to 3, ......— v ~ yz ”n — a - Cu- j - t- -c-- £ —s- e t - t-m -, - that .contains, .standard conclusive field with xylidyl blue as an indicator of magnesium cations (mmol / l Mg ² * / xylidylM molar ratio blue to recognize reduced excretion of 1 to 2, xylidyl blue concentration of 1 to 10 mmol / l of loading solution, pH 11 to 12 buffer, for example Good, a reference interval of magnesium cations in urine of 2 to 6 mol / l, decreased excretion <1 mmol / l in urine). The test strip according to any one of claims 1 to 4. characterized in that it contains a standard detection field with o-cresolphthalein complexone as an indicator of calcium cations (o-cresolphthalein concentration 100 to 1000 phol / l of the impregnation solution, Ca ²⁺ / cresolphthalein 10 to 100 ratio, pH 10 - e.g., Good buffer, a reference urinary calcium cation interval of 1 to 9 mmol / l to recognize increased calcium cation excretion (> 10 mmol / l in urine). Test strip according to one of claims 1 to 5, -v_y_z_ n_a_ č_u_g_í_c_í_se that it contains significant standardní_ box with ammonium molybdate / vanadate as indicator monohydrogenfosfátové anions (molar ratio in mmol / 1 HP0 ^J \ / molybdate to discern elevated excretion 1-2, molybdate concentration of 10-50 mmol / 1 Inlet solution, buffer pH 1 to 3, reference interval in urine 10 to 40 mmol / l, increased excretion> 30 mmol / l in urine). Test strip according to one of Claims 1 to 6, characterized in that it contains a standard detection field with Ferro Zine as an iron ( ^III ) cation indicator (Fe ^{I +} / Ferro Zine molar ratio at zero / 1 to recognize an increased excretion of 0.1 to 0.01, Ferro Zine concentration 50 to 500 ng / l of impregnation solution, pH 4-6 buffer,