CY1114955T1 - SMALL RNAs Molecules That Cause RNA Interference - Google Patents

SMALL RNAs Molecules That Cause RNA Interference

Info

Publication number
CY1114955T1
CY1114955T1 CY20131101154T CY131101154T CY1114955T1 CY 1114955 T1 CY1114955 T1 CY 1114955T1 CY 20131101154 T CY20131101154 T CY 20131101154T CY 131101154 T CY131101154 T CY 131101154T CY 1114955 T1 CY1114955 T1 CY 1114955T1
Authority
CY
Cyprus
Prior art keywords
rna
generated
dsrna
rna interference
sequence
Prior art date
Application number
CY20131101154T
Other languages
Greek (el)
Inventor
Thomas Tuschl
Sayda Elbashir
Winfried Lendeckel
Matthias Wilm
Reinhard Lührmann
Original Assignee
MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP10179947.6A external-priority patent/EP2351852B2/en
Application filed by MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. filed Critical MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V.
Publication of CY1114955T1 publication Critical patent/CY1114955T1/en

Links

Landscapes

  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Διπλόκλωνο RΝΑ (dsRΝΑ) προκαλεί αλληλουχίας-ειδική μετα-μεταγραφική γονιδίου αποσιώπηση σε πολλούς οργανισμούς με μια μέθοδο γνωστή ως RΝΑ παρέμβαση (RΝΑi). Χρησιμοποιώντας ένα in vitro σύστημα Drosophila, καταδείξαμε ότι 19-23 nt βραχέως RΝΑ θραύσματα είναι οι αλληλουχίας-ειδικοί μεσολαβητές της RΝΑi. Τα βραχέα παρεμβαίνοντα RΝΑs (siRΝΑs) δημιουργούνται από μια αντίδραση επεξεργασίας, που ομοιάζει με RΝάση III από μακρύ dsRΝΑ. Χημικώς συντιθέμενα siRΝΑ δίπολα με προεξέχοντα 3' άκρα προκαλούν ικανοποιητική διάσπαση RΝΑ στόχου στο προϊόν λύσης και η θέση διάσπασης είναι τοποθετημένη πλησίον του κέντρου της περιοχής που καλύπτεται από τον οδηγό siRΝΑ. Περαιτέρω, παρέχουμε απόδειξη ότι η κατεύθυνση της dsRΝΑ επεξεργασίας προσδιορίζει κατά πόσο RΝΑ στόχος με νόημα ή αντινόημα μπορεί να διασπάται από το παραγόμενο siRNP σύμπλοκο.Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a method known as RNA interference (RNA). Using an in vitro Drosophila system, we demonstrated that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. Short interfering RNAs (siRNAs) are generated by a processing reaction that resembles RNase III from long dsRNAs. Chemically synthesized siRNA RNAs with protruding 3 'ends cause satisfactory cleavage of target RNA in the lysate and the cleavage site is located near the center of the region covered by the siRNA vector. Further, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the generated siRNP complex.

CY20131101154T 2000-12-01 2013-12-20 SMALL RNAs Molecules That Cause RNA Interference CY1114955T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00126325 2000-12-01
US27966101A 2001-03-30 2001-03-30
EP10179947.6A EP2351852B2 (en) 2000-12-01 2001-11-29 RNA interference mediating small RNA molecules

Publications (1)

Publication Number Publication Date
CY1114955T1 true CY1114955T1 (en) 2016-12-14

Family

ID=58461595

Family Applications (4)

Application Number Title Priority Date Filing Date
CY20071101616T CY1117311T1 (en) 2000-12-01 2007-12-19 SMALL RNAs Molecules That Cause RNA Interference
CY20131101154T CY1114955T1 (en) 2000-12-01 2013-12-20 SMALL RNAs Molecules That Cause RNA Interference
CY20141100524T CY1115279T1 (en) 2000-12-01 2014-07-11 SMALL RNA CIRCUITS CAUSING RNA INTERFERENCE
CY20141100798T CY1115584T1 (en) 2000-12-01 2014-10-03 SMALL RNA CIRCUITS CAUSING RNA INTERFERENCE

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CY20071101616T CY1117311T1 (en) 2000-12-01 2007-12-19 SMALL RNAs Molecules That Cause RNA Interference

Family Applications After (2)

Application Number Title Priority Date Filing Date
CY20141100524T CY1115279T1 (en) 2000-12-01 2014-07-11 SMALL RNA CIRCUITS CAUSING RNA INTERFERENCE
CY20141100798T CY1115584T1 (en) 2000-12-01 2014-10-03 SMALL RNA CIRCUITS CAUSING RNA INTERFERENCE

Country Status (1)

Country Link
CY (4) CY1117311T1 (en)

Also Published As

Publication number Publication date
CY1115584T1 (en) 2017-01-04
CY1117311T1 (en) 2017-04-26
CY1115279T1 (en) 2017-01-04

Similar Documents

Publication Publication Date Title
CY1119062T1 (en) SMALL RNAs Molecules That Cause RNA Interference
DK2361981T4 (en) RNA SEQUENCE SPECIFIC MEDIATORS OF RNA INTERFERENCE
CY1114955T1 (en) SMALL RNAs Molecules That Cause RNA Interference