CY1114446T1 - Μεθοδος διαχωρισμου, ταυτοποιησεως και ποσοτικοποιησεως των αλκαλικων ισοφωσφατασων με ηλεκτροφορηση - Google Patents
Μεθοδος διαχωρισμου, ταυτοποιησεως και ποσοτικοποιησεως των αλκαλικων ισοφωσφατασων με ηλεκτροφορησηInfo
- Publication number
- CY1114446T1 CY1114446T1 CY20131100846T CY131100846T CY1114446T1 CY 1114446 T1 CY1114446 T1 CY 1114446T1 CY 20131100846 T CY20131100846 T CY 20131100846T CY 131100846 T CY131100846 T CY 131100846T CY 1114446 T1 CY1114446 T1 CY 1114446T1
- Authority
- CY
- Cyprus
- Prior art keywords
- electrophoresis
- alkaline
- islosphosphates
- quantitation
- electricity
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Η εφεύρεση αφορά μία μέθοδο διαχωρισμού με ηλεκτροφόρηση, εκκινώντας από ένα βιολογικό δείγμα, των ισοενζύμων της αλκαλικής φωσφατάσης, που χαρακτηρίζεται από το ότι η ηλεκτροφόρηση εκτελείται επί ενός υποστρώματος ηλεκτροφορήσεως μετά από απόθεση ενός διαλύματος λεκτίνης σε μία εντοπισμένη καθορισμένη ζώνη του υποστρώματος ηλεκτροφορήσεως, υπό συνθήκες που επιτρέπουν την αλληλεπίδραση μεταξύ της εν λόγω λεκτίνης και των ισοενζύμων της ΑLΡ που περιέχονται στο αναλυόμενο βιολογικό δείγμα, όπου η απόθεση του διαλύματος λεκτίνης εκτελείται επιπλέον υπό συνθήκες κατάλληλες ώστε να επιτραπεί ο διαχωρισμός των ισοενζύμων της ΑLΡ που συνίστανται από το οστικό κλάσμα και από το ηπατικό κλάσμα.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0003142A FR2806161B1 (fr) | 2000-03-10 | 2000-03-10 | Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese |
EP01907837.7A EP1204862B8 (fr) | 2000-03-10 | 2001-02-16 | Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1114446T1 true CY1114446T1 (el) | 2016-10-05 |
Family
ID=8847988
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20131100846T CY1114446T1 (el) | 2000-03-10 | 2013-10-02 | Μεθοδος διαχωρισμου, ταυτοποιησεως και ποσοτικοποιησεως των αλκαλικων ισοφωσφατασων με ηλεκτροφορηση |
Country Status (12)
Country | Link |
---|---|
US (2) | US6231736B1 (el) |
EP (1) | EP1204862B8 (el) |
JP (1) | JP4871477B2 (el) |
AR (1) | AR027629A1 (el) |
AU (1) | AU3571201A (el) |
CY (1) | CY1114446T1 (el) |
DK (1) | DK1204862T3 (el) |
ES (1) | ES2430188T3 (el) |
FR (1) | FR2806161B1 (el) |
HK (1) | HK1046035A1 (el) |
PT (1) | PT1204862E (el) |
WO (1) | WO2001069229A2 (el) |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE23363T1 (de) * | 1983-01-12 | 1986-11-15 | Boehringer Mannheim Gmbh | Verfahren und reagenz zur differenzierten bestimmung von isoenzymen der alkalischen phosphatase. |
ATE131745T1 (de) * | 1990-04-11 | 1996-01-15 | Ludwig Inst Cancer Res | Verfahren und gerät zum folgerichtigen chemischen reaktionsablauf |
FR2671290B1 (fr) | 1991-01-04 | 1993-04-16 | Sebia Sa | Dispositif pour l'application d'echantillons biologiques sur une plaque d'electrophorese. |
BE1005220A3 (fr) | 1991-01-15 | 1993-06-01 | Analis Sa | Procede de separation, d'identification et de quantification d'isoenzymes et d'isoformes de la phosphatase alcaline. |
WO1995023245A1 (en) * | 1994-02-25 | 1995-08-31 | Labintelligence, Inc. | Real time in-gel assay |
EP0848251A3 (en) * | 1996-12-16 | 1999-06-30 | Beckman Coulter, Inc. | Homogeneous on-line assays using capillary electrophoresis |
CA2322975A1 (en) * | 1998-03-03 | 1999-09-10 | Mosaic Technologies | Purification and detection processes using reversible affinity electrophoresis |
-
2000
- 2000-03-10 FR FR0003142A patent/FR2806161B1/fr not_active Expired - Fee Related
- 2000-06-19 US US09/597,382 patent/US6231736B1/en not_active Expired - Lifetime
-
2001
- 2001-02-08 US US09/779,110 patent/US6391176B2/en not_active Expired - Lifetime
- 2001-02-16 AU AU35712/01A patent/AU3571201A/en not_active Abandoned
- 2001-02-16 JP JP2001568061A patent/JP4871477B2/ja not_active Expired - Fee Related
- 2001-02-16 DK DK01907837.7T patent/DK1204862T3/da active
- 2001-02-16 ES ES01907837T patent/ES2430188T3/es not_active Expired - Lifetime
- 2001-02-16 WO PCT/FR2001/000479 patent/WO2001069229A2/fr active Application Filing
- 2001-02-16 EP EP01907837.7A patent/EP1204862B8/fr not_active Expired - Lifetime
- 2001-02-16 PT PT1907837T patent/PT1204862E/pt unknown
- 2001-03-09 AR ARP010101110A patent/AR027629A1/es unknown
-
2002
- 2002-10-17 HK HK02107582.7A patent/HK1046035A1/zh unknown
-
2013
- 2013-10-02 CY CY20131100846T patent/CY1114446T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
ES2430188T3 (es) | 2013-11-19 |
JP2003527600A (ja) | 2003-09-16 |
EP1204862B8 (fr) | 2013-09-11 |
DK1204862T3 (da) | 2013-10-21 |
HK1046035A1 (zh) | 2002-12-20 |
EP1204862A2 (fr) | 2002-05-15 |
FR2806161A1 (fr) | 2001-09-14 |
PT1204862E (pt) | 2013-10-01 |
AR027629A1 (es) | 2003-04-02 |
AU3571201A (en) | 2001-09-24 |
WO2001069229A3 (fr) | 2002-03-07 |
WO2001069229A2 (fr) | 2001-09-20 |
JP4871477B2 (ja) | 2012-02-08 |
US20010029016A1 (en) | 2001-10-11 |
US6391176B2 (en) | 2002-05-21 |
FR2806161B1 (fr) | 2002-09-20 |
EP1204862B1 (fr) | 2013-07-10 |
US6231736B1 (en) | 2001-05-15 |
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