CU24452B1 - ELECTRONIC SYSTEM FOR THE DETECTION OF MICROORGANISMS IN BIOLOGICAL SAMPLES BY CONTROLLED PHOTOSTIMULATION - Google Patents

ELECTRONIC SYSTEM FOR THE DETECTION OF MICROORGANISMS IN BIOLOGICAL SAMPLES BY CONTROLLED PHOTOSTIMULATION

Info

Publication number
CU24452B1
CU24452B1 CU2016000070A CU20160070A CU24452B1 CU 24452 B1 CU24452 B1 CU 24452B1 CU 2016000070 A CU2016000070 A CU 2016000070A CU 20160070 A CU20160070 A CU 20160070A CU 24452 B1 CU24452 B1 CU 24452B1
Authority
CU
Cuba
Prior art keywords
photostimulation
detection
biological samples
microorganisms
controlled
Prior art date
Application number
CU2016000070A
Other languages
Spanish (es)
Other versions
CU20160070A7 (en
Inventor
Alarcón Orestes Rolando Contreras
Nuviola Carlos Abel Lamothe
Barrios Carmen Yamilé Moreno
Frómeta Nardo Ramírez
Gómez Ángel Regueiro
Rodríguez Elier Riverón
Original Assignee
Centro Nac De Investigaciones Cientificas Biocubafarma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro Nac De Investigaciones Cientificas Biocubafarma filed Critical Centro Nac De Investigaciones Cientificas Biocubafarma
Priority to PCT/CU2017/050004 priority Critical patent/WO2017198240A1/en
Priority to CU2016000070A priority patent/CU24452B1/en
Publication of CU20160070A7 publication Critical patent/CU20160070A7/en
Publication of CU24452B1 publication Critical patent/CU24452B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/53—Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke
    • G01N21/534—Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke by measuring transmission alone, i.e. determining opacity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G01N21/5907—Densitometers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

<p>Esta invención relacionada con Ia rama de Ia Microbiología e Higiene de los Alimentos, constituye un sistema para Ia evaluación rápida del estado de contaminación microbiológica de diferentes muestras biológicas a partir del empleo combinado de Ia fotoestimulación-detección de los microorganismos (fotosintéticos y no fotosintéticos) presentes en un medio de cultivo líquido.</p> <p>En el caso de Ia fotoestimulación (430 nm < λ < 480 nm y 1 µW< p < 5 µW) de una muestra biológica en medio líquido, se registran los cambios de turbidez y/o bioimpedancia originados en el medio de cultivo por dicho crecimiento, lo cual permite detectar de forma rápida Ia presencia de bacterias en muestras biológicas a partir del análisis del cultivo, facilitando Ia evaluación de Ia calidad microbiológica de las mismas. Además es útil para Ia determinación del antibiograma a partir de colonias aisladas o de muestras positivas que se obtienen directamente de las fuentes que las contienen.</p><p> This invention related to the branch of Food Microbiology and Hygiene, constitutes a system for the rapid evaluation of the state of microbiological contamination of different biological samples based on the combined use of photostimulation-detection of microorganisms (photosynthetic and non-photosynthetic) present in a liquid culture medium. </p> <p> In the case of photostimulation (430 nm <λ <480 nm and 1 µW <p <5 µW) of a biological sample in liquid medium, record the changes in turbidity and / or bioimpedance caused in the culture medium by said growth, which allows for the rapid detection of the presence of bacteria in biological samples from the analysis of the culture, facilitating the evaluation of the microbiological quality of the same . It is also useful for determining the antibiogram from isolated colonies or positive samples obtained directly from the sources that contain them. </p>

CU2016000070A 2016-05-19 2017-05-18 ELECTRONIC SYSTEM FOR THE DETECTION OF MICROORGANISMS IN BIOLOGICAL SAMPLES BY CONTROLLED PHOTOSTIMULATION CU24452B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/CU2017/050004 WO2017198240A1 (en) 2016-05-19 2017-05-18 System for the detection of photosynthetic and non-photosynthetic microorganisms in biological samples by means of controlled photostimulation
CU2016000070A CU24452B1 (en) 2016-05-19 2017-05-18 ELECTRONIC SYSTEM FOR THE DETECTION OF MICROORGANISMS IN BIOLOGICAL SAMPLES BY CONTROLLED PHOTOSTIMULATION

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU2016000070A CU24452B1 (en) 2016-05-19 2017-05-18 ELECTRONIC SYSTEM FOR THE DETECTION OF MICROORGANISMS IN BIOLOGICAL SAMPLES BY CONTROLLED PHOTOSTIMULATION

Publications (2)

Publication Number Publication Date
CU20160070A7 CU20160070A7 (en) 2017-12-08
CU24452B1 true CU24452B1 (en) 2019-11-04

Family

ID=59284951

Family Applications (1)

Application Number Title Priority Date Filing Date
CU2016000070A CU24452B1 (en) 2016-05-19 2017-05-18 ELECTRONIC SYSTEM FOR THE DETECTION OF MICROORGANISMS IN BIOLOGICAL SAMPLES BY CONTROLLED PHOTOSTIMULATION

Country Status (2)

Country Link
CU (1) CU24452B1 (en)
WO (1) WO2017198240A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT117223A (en) 2021-05-12 2022-11-14 Inst Politecnico De Leiria MICROBIOLOGICAL DETECTION PROCESS AND DETERMINATION OF ANTIMICROBIAL SUSCEPTIBILITY IN CLINICAL, ENVIRONMENTAL OR FOOD SAMPLES

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR035231A1 (en) * 2002-03-11 2004-05-05 Ypf S A A TEAM TO ANALYZE THE GROWTH OF MICROORGANISMS AND PROCEDURE TO QUANTIFY THE CONCENTRATION OF MICROORGANISMS
US7328708B2 (en) 2003-12-23 2008-02-12 United Laboratories & Manufacturing, Llc LED multiplex source and method of use of for sterilization, bioactivation and therapy
KR20080086988A (en) * 2005-12-09 2008-09-29 바이오나비타스, 인크. System, devices, and methods for biomass production
GB0701517D0 (en) 2007-01-26 2007-03-07 Univ Leicester Growth inducer
IT1392994B1 (en) * 2009-02-25 2012-04-02 Alifax Holding S P A PROCEDURE FOR BACTERIOLOGICAL SURVEY ON A BIOLOGICAL SAMPLE AND ITS DEVICE
WO2010132955A1 (en) * 2009-05-21 2010-11-25 Omega 3 Innovations Pty Ltd Apparatus, system and method for photosynthesis
EP2668259B1 (en) * 2011-01-28 2017-03-29 Algaecytes Limited Process for production of microalgae, cyanobacteria and metabolites thereof
US9506073B2 (en) 2011-11-18 2016-11-29 Board Of Regents, The University Of Texas System Blue-light inducible system for gene expression
TW201330896A (en) 2012-01-19 2013-08-01 Forward Electronics Co Ltd Photo-stimulation method and device
CN103212161A (en) 2012-01-19 2013-07-24 福华电子股份有限公司 Photostimulation method and photostimulation device
FR2997704B1 (en) 2012-11-07 2017-08-11 Olivier Girinsky USE OF BLUE LIGHT FOR THE STIMULATION OF METABOLISM OF NON-PHOTOTROPHIC MICROORGANISMS

Also Published As

Publication number Publication date
CU20160070A7 (en) 2017-12-08
WO2017198240A1 (en) 2017-11-23

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