CU22729A1 - PROCEDURE FOR THE OBTAINING OF SUPERES HYPERINMUNES ANTIESPECIES FOR THE IDENTIFICATION OF BIOLOGICAL FABRICS - Google Patents

PROCEDURE FOR THE OBTAINING OF SUPERES HYPERINMUNES ANTIESPECIES FOR THE IDENTIFICATION OF BIOLOGICAL FABRICS

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Publication number
CU22729A1
CU22729A1 CU1998127A CU1998127A CU22729A1 CU 22729 A1 CU22729 A1 CU 22729A1 CU 1998127 A CU1998127 A CU 1998127A CU 1998127 A CU1998127 A CU 1998127A CU 22729 A1 CU22729 A1 CU 22729A1
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CU
Cuba
Prior art keywords
obtaining
superes
hyperinmunes
antiespecies
identification
Prior art date
Application number
CU1998127A
Other languages
Spanish (es)
Inventor
Hernandez Rosario Iserns
Rodriguez Mario Valdes
Original Assignee
Ct Provincial De Producciones Agropecuarias
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Ct Provincial De Producciones Agropecuarias filed Critical Ct Provincial De Producciones Agropecuarias
Priority to CU1998127A priority Critical patent/CU22729A1/en
Publication of CU22729A1 publication Critical patent/CU22729A1/en

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Abstract

La presente invención se refiere al procedimiento para obtener IgG (anticuerpos policlonales) utilizando antígenos de diferentes especies en carneros y conejos, luego de identifican cada animal se utiliza un ejemplar para cada inmunógeno empleando un esquema de inmunizacíón de larga duración y concentraciones de proteínas adecuadas. En el momento adecuado se realizan extracciones de sangre. Se procesa hasta obtener el suero, posterior a su clarificación se somete a un proceso de purificación con Sulfato de Amonio, seguidamente la desalinización mediante un proceso de diálisis. Estos sueros son utilizados como medio de diagnóstico en los métodos de inmunodifusión radial, electroforesis en gel de agar y en la contrainmunoelectroforesis donde se conjuga el principio de la electroforesis y las reacciones antígeno - anticuerpo en medio gelificado.The present invention relates to the process for obtaining IgG (polyclonal antibodies) using antigens of different species in rams and rabbits, after identifying each animal a copy is used for each immunogen using a long-term immunization scheme and adequate protein concentrations. Blood withdrawals are performed at the right time. It is processed until the serum is obtained, after its clarification it is subjected to a purification process with Ammonium Sulfate, then the desalination through a dialysis process. These sera are used as diagnostic means in the methods of radial immunodiffusion, agar gel electrophoresis and in the counterimmunoelectrophoresis where the principle of electrophoresis and antigen-antibody reactions in gelled medium are conjugated.

CU1998127A 1998-09-11 1998-09-11 PROCEDURE FOR THE OBTAINING OF SUPERES HYPERINMUNES ANTIESPECIES FOR THE IDENTIFICATION OF BIOLOGICAL FABRICS CU22729A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CU1998127A CU22729A1 (en) 1998-09-11 1998-09-11 PROCEDURE FOR THE OBTAINING OF SUPERES HYPERINMUNES ANTIESPECIES FOR THE IDENTIFICATION OF BIOLOGICAL FABRICS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1998127A CU22729A1 (en) 1998-09-11 1998-09-11 PROCEDURE FOR THE OBTAINING OF SUPERES HYPERINMUNES ANTIESPECIES FOR THE IDENTIFICATION OF BIOLOGICAL FABRICS

Publications (1)

Publication Number Publication Date
CU22729A1 true CU22729A1 (en) 2002-02-28

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Family Applications (1)

Application Number Title Priority Date Filing Date
CU1998127A CU22729A1 (en) 1998-09-11 1998-09-11 PROCEDURE FOR THE OBTAINING OF SUPERES HYPERINMUNES ANTIESPECIES FOR THE IDENTIFICATION OF BIOLOGICAL FABRICS

Country Status (1)

Country Link
CU (1) CU22729A1 (en)

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