CU22706A1 - INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED - Google Patents
INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINEDInfo
- Publication number
- CU22706A1 CU22706A1 CU1999183A CU1999183A CU22706A1 CU 22706 A1 CU22706 A1 CU 22706A1 CU 1999183 A CU1999183 A CU 1999183A CU 1999183 A CU1999183 A CU 1999183A CU 22706 A1 CU22706 A1 CU 22706A1
- Authority
- CU
- Cuba
- Prior art keywords
- purification
- hermodice
- carunculata
- marine
- inhibitor
- Prior art date
Links
Abstract
Se establece por primera vez un procedimiento de aislamiento y purificación de un inhibidor natural de piroglutamil amonopeptidasa II a partir de extractos del anélido marino Hermodice carunculata. El protocolo consta de tres etapas fundamentales: Tratamiento del extracto con ácido tricloroacético al 2.5%, Cromatografía del Intercambio Aniónico en DEAE-Sephacel y Cromatografía de Exclusión Molecular en Sephadex G-25. La homogeneidad de la fracción obtenida (Rendimientos mayores del 80 % y Grado de Purificación elevados), fue comprobada por la obtención de un solo pico estrecho y simétrico en RP-HPLC y la obtención de una sola banda electroforética en geles de poliacrilamida SDS-PAGE.A procedure of isolation and purification of a natural pyroglutamyl amonopeptidase II inhibitor from extracts of marine anhydride Hermodice carunculata is established for the first time. The protocol consists of three fundamental stages: Treatment of the extract with 2.5% trichloroacetic acid, Anion Exchange Chromatography in DEAE-Sephacel and Molecular Exclusion Chromatography in Sephadex G-25. The homogeneity of the fraction obtained (yields greater than 80% and high degree of Purification), was verified by obtaining a single narrow and symmetrical peak in RP-HPLC and obtaining a single electrophoretic band in polyacrylamide gels SDS-PAGE .
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU1999183A CU22706A1 (en) | 1999-11-01 | 1999-11-01 | INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU1999183A CU22706A1 (en) | 1999-11-01 | 1999-11-01 | INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED |
Publications (1)
Publication Number | Publication Date |
---|---|
CU22706A1 true CU22706A1 (en) | 2001-07-31 |
Family
ID=46599360
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CU1999183A CU22706A1 (en) | 1999-11-01 | 1999-11-01 | INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED |
Country Status (1)
Country | Link |
---|---|
CU (1) | CU22706A1 (en) |
-
1999
- 1999-11-01 CU CU1999183A patent/CU22706A1/en unknown
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