CU22706A1 - INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED - Google Patents

INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED

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Publication number
CU22706A1
CU22706A1 CU1999183A CU1999183A CU22706A1 CU 22706 A1 CU22706 A1 CU 22706A1 CU 1999183 A CU1999183 A CU 1999183A CU 1999183 A CU1999183 A CU 1999183A CU 22706 A1 CU22706 A1 CU 22706A1
Authority
CU
Cuba
Prior art keywords
purification
hermodice
carunculata
marine
inhibitor
Prior art date
Application number
CU1999183A
Other languages
Spanish (es)
Inventor
Cisneros Miguel Ramirez
Casalonga Jean Louis Charli
Brito Joaquin Diaz
Parrado Shirley Gil
Alonso Isel Pascual
Planes Maria De Los Angeles Chavez
Original Assignee
Univ La Habana
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ La Habana filed Critical Univ La Habana
Priority to CU1999183A priority Critical patent/CU22706A1/en
Publication of CU22706A1 publication Critical patent/CU22706A1/en

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Abstract

Se establece por primera vez un procedimiento de aislamiento y purificación de un inhibidor natural de piroglutamil amonopeptidasa II a partir de extractos del anélido marino Hermodice carunculata. El protocolo consta de tres etapas fundamentales: Tratamiento del extracto con ácido tricloroacético al 2.5%, Cromatografía del Intercambio Aniónico en DEAE-Sephacel y Cromatografía de Exclusión Molecular en Sephadex G-25. La homogeneidad de la fracción obtenida (Rendimientos mayores del 80 % y Grado de Purificación elevados), fue comprobada por la obtención de un solo pico estrecho y simétrico en RP-HPLC y la obtención de una sola banda electroforética en geles de poliacrilamida SDS-PAGE.A procedure of isolation and purification of a natural pyroglutamyl amonopeptidase II inhibitor from extracts of marine anhydride Hermodice carunculata is established for the first time. The protocol consists of three fundamental stages: Treatment of the extract with 2.5% trichloroacetic acid, Anion Exchange Chromatography in DEAE-Sephacel and Molecular Exclusion Chromatography in Sephadex G-25. The homogeneity of the fraction obtained (yields greater than 80% and high degree of Purification), was verified by obtaining a single narrow and symmetrical peak in RP-HPLC and obtaining a single electrophoretic band in polyacrylamide gels SDS-PAGE .

CU1999183A 1999-11-01 1999-11-01 INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED CU22706A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CU1999183A CU22706A1 (en) 1999-11-01 1999-11-01 INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1999183A CU22706A1 (en) 1999-11-01 1999-11-01 INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED

Publications (1)

Publication Number Publication Date
CU22706A1 true CU22706A1 (en) 2001-07-31

Family

ID=46599360

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1999183A CU22706A1 (en) 1999-11-01 1999-11-01 INSULATION AND PURIFICATION OF A PIROGLUTAMIL AMINOPEPTIDASA II INHIBITOR FROM THE MARINE ANELID HERMODICE CARUNCULATA AND THE PRODUCT SO OBTAINED

Country Status (1)

Country Link
CU (1) CU22706A1 (en)

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