CU22288A1 - Hyperprotective recombining yeast of the heat-stable alpha amilase enzyme, and the procedure for obtaining it. - Google Patents

Hyperprotective recombining yeast of the heat-stable alpha amilase enzyme, and the procedure for obtaining it.

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Publication number
CU22288A1
CU22288A1 CU1990142A CU1990142A CU22288A1 CU 22288 A1 CU22288 A1 CU 22288A1 CU 1990142 A CU1990142 A CU 1990142A CU 1990142 A CU1990142 A CU 1990142A CU 22288 A1 CU22288 A1 CU 22288A1
Authority
CU
Cuba
Prior art keywords
yeast
enzyme
amylase
recombining
procedure
Prior art date
Application number
CU1990142A
Other languages
Spanish (es)
Inventor
Reyes Edenia Paifer
Clark Emilio Margolles
Boada Julio Marcos Delgado
Segui Raquel Montesinos
Alvarez Jose Gilberto Cremata
Martinez Luis Saturnino Herrera
Original Assignee
Cigb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cigb filed Critical Cigb
Priority to CU1990142A priority Critical patent/CU22288A1/en
Priority to AT91200074T priority patent/ATE220721T1/en
Priority to JP3014953A priority patent/JPH05184352A/en
Priority to DE69133066T priority patent/DE69133066T2/en
Priority to ES91200074T priority patent/ES2180528T3/en
Priority to EP91200074A priority patent/EP0438200B1/en
Priority to DK91200074T priority patent/DK0438200T3/en
Publication of CU22288A1 publication Critical patent/CU22288A1/en

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE RELACIONA CON EL CAMPO DE LA BIOTECNOLOGIA Y LA TECNOLOGIA DEL ADN RECOMBINANTE Y EN PARTICULAR CON UNA NUEVA CEPA RECOMBINANTE DE LEVADURA PRODUCTORA DE ALFA AMILASA ASI COMO CON EL PROCEDIMIENTO PARA SU OBTENCION. EL OBJETIVO TECNICO QUE SE PERSIGUE ES LA CONSTRUCCION DE UNA CEPA DE LEVADURA RECOMBINANTE CAPAZ DE PRODUCIR LA ENZIMA ALFA AMILASA TERMOESTABLE LA CUAL PRESENTA UN SISTEMA MUY EFICIENTE DE REGULACION DEL PRODUCTO GENICO DESEADO, ASI COMO LA CAPACIDAD DE EXCRETAR CANTIDADES SUSTANCIALES DE LA ENZIMA AL MEDIO DE CULTIVO. ESTO SE LOGRA TRANSFORMANDO LA LEVADURA DEL GENERO PICHIA PASTORIS MUTANTE HIS- CON UN PLASMIDO PREPARADO POR INGENIERIA GENETICA PARA ESTE FIN, EN EL CUAL SE ENCUENTRA EL GEN ALFA AMILASA DE BACILLUS LICHENIFORMIS, ACOPLADO A LA SECUENCIA SEÑAL DEL GEN SACAROSA INVERTASA Y REGULADO POR EL PROMOTOR DEL GEN ALCOHOL OXIDASA DE LA LEVADURA. ESTA LEVADURA TRANSFORMADA, ES CAPAZ DE PRODUCIR ALTOS NIVELES DE ALTA AMILASA BAJO DETERMINADAS CONDICIONES EN U N PROCESO DE FERMENTACION-INDUCCION. DEBIDO A LAS CARACTERISTICAS DE ESTA LEVADURA Y LA INOCUIDAD DE LA MISMA, EL PREPARADO COMERCIAL DE ALTA AMILASA PUEDE SER UTILIZADO EN EL CAMPO DE LA INDUSTRIA AZUCARERA, ALIMENTICIA Y TEXTIL, LAS CUALES REQUIEREN DE LA HIDROLISIS DE LOS ALMIDONES PRESENTES EN ESOS PROCESOS INDUSTRIALES A PARTIR DE LA UTILIZACION DE DICHA ENZIMA.\!IT IS RELATED TO THE FIELD OF BIOTECHNOLOGY AND RECOMBINANT DNA TECHNOLOGY AND IN PARTICULAR WITH A NEW RECOMBINING STRAIN OF ALFA AMYLASE PRODUCING AS WELL AS THE PROCEDURE FOR ITS OBTAINING. THE TECHNICAL OBJECTIVE THAT IS PURSUED IS THE CONSTRUCTION OF A RECOMBINANT YEAST STRAIN CAPABLE OF PRODUCING THE THERMOSTABLE ALPHA ALPHAMYLASE ENZYME, WHICH PRESENTS A VERY EFFICIENT SYSTEM FOR REGULATION OF THE DESIRED GENE PRODUCT IN LA SCIENTIST EXCRETARIUM. OF CULTIVATION. THIS IS ACHIEVED BY TRANSFORMING THE YEAST OF THE GENUS PICHIA PASTORIS MUTANTE HIS- WITH A PLASMID PREPARED BY GENETIC ENGINEERING FOR THIS PURPOSE, IN WHICH THE ALPHA AMYLASE GENE OF BACILLUS REGULAR SEA SIGNAL IS FOUND, LINKED TO THE SIGNAL SEQUAL REGULATION PROMOTER OF THE GENE ALCOHOL OXIDASE OF YEAST. THIS TRANSFORMED YEAST IS ABLE TO PRODUCE HIGH LEVELS OF HIGH AMYLASE UNDER CERTAIN CONDITIONS IN A FERMENTATION-INDUCTION PROCESS. DUE TO THE CHARACTERISTICS OF THIS YEAST AND THE SAFETY OF IT, THE COMMERCIAL PREPARATION OF HIGH AMYLASE CAN BE USED IN THE FIELD OF THE SUGAR, FOOD AND TEXTILE INDUSTRY, WHICH REQUIRE THE HYDROLYSIS PRESENT IN INDUSTRIAL STARCHES STARTING FROM THE USE OF SUCH ENZYME. \!

CU1990142A 1990-01-16 1990-08-15 Hyperprotective recombining yeast of the heat-stable alpha amilase enzyme, and the procedure for obtaining it. CU22288A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CU1990142A CU22288A1 (en) 1990-08-15 1990-08-15 Hyperprotective recombining yeast of the heat-stable alpha amilase enzyme, and the procedure for obtaining it.
AT91200074T ATE220721T1 (en) 1990-01-16 1991-01-16 METHOD FOR EXPRESSING HETEROLOGICAL GENES IN THE YEAST PICHIA PASTORIS, EXPRESSION VECTORS AND TRANSFORMED MICROORGANISMS
JP3014953A JPH05184352A (en) 1990-01-16 1991-01-16 Method for expressing dissimilar gene in pichia pastoris yeast, expression vector and transforming microorganism
DE69133066T DE69133066T2 (en) 1990-01-16 1991-01-16 Process for the expression of heterological genes in the yeast Pichia Pastoris, expression process and transformed microorganisms
ES91200074T ES2180528T3 (en) 1990-01-16 1991-01-16 METHOD OF EXPRESSION OF HETEROLOGICAL GENES IN PICHIA PASTORIS YEAST, EXPRESSION VECTORS AND TRANSFORMED MICROORGANISMS.
EP91200074A EP0438200B1 (en) 1990-01-16 1991-01-16 Method for the expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms
DK91200074T DK0438200T3 (en) 1990-01-16 1991-01-16 Method for expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1990142A CU22288A1 (en) 1990-08-15 1990-08-15 Hyperprotective recombining yeast of the heat-stable alpha amilase enzyme, and the procedure for obtaining it.

Publications (1)

Publication Number Publication Date
CU22288A1 true CU22288A1 (en) 1995-01-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CU1990142A CU22288A1 (en) 1990-01-16 1990-08-15 Hyperprotective recombining yeast of the heat-stable alpha amilase enzyme, and the procedure for obtaining it.

Country Status (1)

Country Link
CU (1) CU22288A1 (en)

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