CU22278A1 - A recombining strain of yeast which produces an aspartic protease which serves to coagulate milk, and the procedure for obtaining it. - Google Patents

A recombining strain of yeast which produces an aspartic protease which serves to coagulate milk, and the procedure for obtaining it.

Info

Publication number
CU22278A1
CU22278A1 CU1990104A CU1990104A CU22278A1 CU 22278 A1 CU22278 A1 CU 22278A1 CU 1990104 A CU1990104 A CU 1990104A CU 1990104 A CU1990104 A CU 1990104A CU 22278 A1 CU22278 A1 CU 22278A1
Authority
CU
Cuba
Prior art keywords
strain
aspartic protease
yeast
procedure
obtaining
Prior art date
Application number
CU1990104A
Other languages
Spanish (es)
Inventor
Grillo Juan Morales
Madrazo Isis Del Carmen Torrens
Santiago Jorge Agustin Aguiar
Binelfa Gerardo Ferbeyre
Barrios Adelaide Villareal
Santana Vladimir Martinez
Espinosa Angela Estela Sosa
Lopez Tania Gonzalez
Martine Luis Saturnino Herrera
Badillo Beatriz Gonzalez
Mesa Jorge Luis Maestre
Rodriguez Alejandro Silva
Original Assignee
Cigb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cigb filed Critical Cigb
Priority to CU1990104A priority Critical patent/CU22278A1/en
Priority to AT91200074T priority patent/ATE220721T1/en
Priority to JP3014953A priority patent/JPH05184352A/en
Priority to DE69133066T priority patent/DE69133066T2/en
Priority to ES91200074T priority patent/ES2180528T3/en
Priority to EP91200074A priority patent/EP0438200B1/en
Priority to DK91200074T priority patent/DK0438200T3/en
Publication of CU22278A1 publication Critical patent/CU22278A1/en

Links

Abstract

SE RELACIONA CON LA RAMA DE LA BIOTECNOLOGIA Y LA INGENIERIA GENETICA, Y EN PARTICULAR CON UNA NUEVA CEPA DE LEVADURA RECOMBINANTE PRODUCTORA DE UNA PROTEASA ASPARTICA, ASI COMO CON EL PROCEDIMIENTO PARA SU OBTENCION. EL OBJETIVO TECNICO QUE PERSIGUE LA MISMA ES LOGRAR UNA CEPA CAPAZ DE EXPRESAR Y EXCRETAR EN CANTIDADES COMERCIALMENTE UTILES, ESTA ENZIMA CON ACTIVIDAD COAGULANTE DE LA LECHE PARA SU EMPLEO EN LA INDUSTRIA QUESERA. PARA LOGRAR ESTE OBJETIVO SE CLONO EL GEN QUE CODIFICA PARA LA PROTEASA ASPARTICA PROVENIENTE DEL HONGO MUCOR PUSILLUS EN LA LEVADURA PICHIA PASTORIS, OBTENIENDOSE UNA CEPA HIPERPRODUCTORA DE LA PROTEINA QUE ALCANZA RENDIMIENTOS DE 7 G/L DE LA MISMA, CUANDO ES INDUCIDA CON METANOL Y EN CONDICIONES DE FERMENTACION SEMICONTINUA. ESTA CEPA PUEDE SER EMPLEADA EN LA PRODUCCION A GRAN ESCALA DE DICHA ENZIMA, LA CUAL ES DE GRAN UTILIDAD PARA LA FABRICACION DE QUESOS.\!IT IS RELATED TO THE BRANCH OF BIOTECHNOLOGY AND GENETIC ENGINEERING, AND PARTICULARLY TO A NEW STRAIN OF RECOMBINANT YEAST PRODUCING AN ASPARTIC PROTEASE, AS WELL AS THE PROCEDURE FOR ITS OBTAINING. THE TECHNICAL OBJECTIVE THAT PURSUES THE SAME IS TO ACHIEVE A STRAIN CAPABLE OF EXPRESSING AND EXCRETING IN COMMERCIALLY USEFUL AMOUNTS, THIS ENZYME WITH COAGULATING ACTIVITY OF MILK FOR ITS USE IN THE CHEESE INDUSTRY. TO ACHIEVE THIS OBJECTIVE, THE GENE THAT CODES FOR ASPARTIC PROTEASE COMING FROM THE MUCOR PUSILLUS FUNGUS IN THE PICHIA PASTORIS YEAST, IS OBTAINED, GETTING A HYPER-PRODUCING STRAIN OF PROTEIN, WHICH REACHES YIELDS OF METAL UNDER SEMI-CONTINUOUS FERMENTATION CONDITIONS. THIS STRAIN CAN BE USED IN THE LARGE-SCALE PRODUCTION OF SUCH ENZYME, WHICH IS OF GREAT UTILITY FOR THE MANUFACTURE OF CHEESES. \!

CU1990104A 1990-01-16 1990-06-13 A recombining strain of yeast which produces an aspartic protease which serves to coagulate milk, and the procedure for obtaining it. CU22278A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CU1990104A CU22278A1 (en) 1990-06-13 1990-06-13 A recombining strain of yeast which produces an aspartic protease which serves to coagulate milk, and the procedure for obtaining it.
AT91200074T ATE220721T1 (en) 1990-01-16 1991-01-16 METHOD FOR EXPRESSING HETEROLOGICAL GENES IN THE YEAST PICHIA PASTORIS, EXPRESSION VECTORS AND TRANSFORMED MICROORGANISMS
JP3014953A JPH05184352A (en) 1990-01-16 1991-01-16 Method for expressing dissimilar gene in pichia pastoris yeast, expression vector and transforming microorganism
DE69133066T DE69133066T2 (en) 1990-01-16 1991-01-16 Process for the expression of heterological genes in the yeast Pichia Pastoris, expression process and transformed microorganisms
ES91200074T ES2180528T3 (en) 1990-01-16 1991-01-16 METHOD OF EXPRESSION OF HETEROLOGICAL GENES IN PICHIA PASTORIS YEAST, EXPRESSION VECTORS AND TRANSFORMED MICROORGANISMS.
EP91200074A EP0438200B1 (en) 1990-01-16 1991-01-16 Method for the expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms
DK91200074T DK0438200T3 (en) 1990-01-16 1991-01-16 Method for expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1990104A CU22278A1 (en) 1990-06-13 1990-06-13 A recombining strain of yeast which produces an aspartic protease which serves to coagulate milk, and the procedure for obtaining it.

Publications (1)

Publication Number Publication Date
CU22278A1 true CU22278A1 (en) 1995-01-31

Family

ID=5459367

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1990104A CU22278A1 (en) 1990-01-16 1990-06-13 A recombining strain of yeast which produces an aspartic protease which serves to coagulate milk, and the procedure for obtaining it.

Country Status (1)

Country Link
CU (1) CU22278A1 (en)

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