CU22054A1 - Method for purifying inhibitors of the sea anemonestaichactis helianthus proteases and the product thus obtained. - Google Patents

Method for purifying inhibitors of the sea anemonestaichactis helianthus proteases and the product thus obtained.

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Publication number
CU22054A1
CU22054A1 CU1988200A CU1988200A CU22054A1 CU 22054 A1 CU22054 A1 CU 22054A1 CU 1988200 A CU1988200 A CU 1988200A CU 1988200 A CU1988200 A CU 1988200A CU 22054 A1 CU22054 A1 CU 22054A1
Authority
CU
Cuba
Prior art keywords
inhibitors
proteases
action
death
purification
Prior art date
Application number
CU1988200A
Other languages
Spanish (es)
Inventor
Planes Maria De Los Angeles Chaves
Cabrera Ileana Martinez
Garcia Julieta A Delfin
Brito Joaquin Diaz
Breto Margarita Marquez
Gonzalez Yamile Gonzalez
Natalia Ivanova Larionova
Novela Fiodorovna Kazanskaya
Original Assignee
Univ La Habana
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ La Habana filed Critical Univ La Habana
Priority to CU1988200A priority Critical patent/CU22054A1/en
Priority to SU894763976A priority patent/RU1789525C/en
Publication of CU22054A1 publication Critical patent/CU22054A1/en

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  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

SE RELACIONA CON LA RAMA DE LA BIOQUIMICA, ESPECIFICAMENTE DE POLIPEPTIDOS INHIBIDORES DE PROTEASAS. EN LA PRESENTE INVENCION SE DESARROLLA UN PROCEDIMIENTO QUE PERMITE LA OBTENCION DE INHIBIDORES DE PROTEASAS DE AMPLIO ESPECTRO DE ACCION, A PARTIR DE LOS EXTRACTOS TOTALES DE LA ANEMONA MARINA STOICHACTIS HELIANTHUS. EL PROCEDIMIENTO DE PURIFICACION CONSISTE EN LA COMBINACION DEL TRATAMIENTO ACIDO TRICLOROACETICO, CROMATOGRAFIA EN SEPHAROSA- TRIPSINA Y CROMATOGRAFIA EN SEPHADEX G-50, AMBAS EN CONDICIONES DETERMINADAS, QUE POSIBILITAN LA OBTENCION CON UN GRADO DE PUREZA SATISFACTORIO, DE TRES INHIBIDORES DE PROTEASAS DE AMPLIO ESPECTRO DE ACCION. LOS INHIBIDORES TIENEN APLICACION EN BIOTECNOLOGIA, ESPECIALMENTE EN LOS PROCEDIMIENTOS DE OBTENCION DE PROTEINAS Y EN LA PURIFICACION DE PROTEASAS POR CROMATOGRAFIA DE AFINIDAD. EL INHIBIDOR OBTENIDO, POR UN AMPLIO ESPECTRO DE ACCION, PERMITE LA PURIFICACION DE PROTEASAS SERINO, CISTEINO, COMO ASPARTICO DEPENDIENTES. SON TAMBIEN UTILIZADO S EN MEDICINA PARA EL TRATAMIENTO DE ENFERMEDADES RELACIONADAS CON UN DESBALANCE PROTEOLISIS INHIBICION.\!IT IS RELATED TO THE BRANCH OF BIOCHEMISTRY, SPECIFICALLY OF PROTEASE INHIBITING POLYPEPTIDES. IN THE PRESENT INVENTION A PROCEDURE IS DEVELOPED THAT ALLOWS THE OBTAINING OF PROTEASE INHIBITORS WITH A WIDE RANGE OF ACTION, FROM THE TOTAL EXTRACTS OF THE MARINE ANEMONE STOICHACTIS HELIANTHUS. THE PURIFICATION PROCEDURE CONSISTS OF THE COMBINATION OF THE TRICLOROACETIC ACID TREATMENT, CHROMATOGRAPHY IN SEPHAROSA- TRIPSINE AND CHROMATOGRAPHY IN SEPHADEX G-50, WHICH HAVE DETERMINED DEATH RATINGS, WHICH HAVE GETTING DEATH-SAFE DEATH. OF ACTION. INHIBITORS HAVE APPLICATION IN BIOTECHNOLOGY, ESPECIALLY IN PROTEIN PROCEDURES AND IN PURIFICATION OF PROTEASES BY AFFINITY CHROMATOGRAPHY. THE INHIBITOR OBTAINED, BY A WIDE SPECTRUM OF ACTION, ALLOWS THE PURIFICATION OF SERINE, CYSTEINE PROTEASES, AS DEPENDENTS ASPARTICAL. THEY ARE ALSO USED IN MEDICINE FOR THE TREATMENT OF DISEASES RELATED TO INHIBITION PROTEOLYSIS IMBALANCE. \!

CU1988200A 1988-12-12 1988-12-12 Method for purifying inhibitors of the sea anemonestaichactis helianthus proteases and the product thus obtained. CU22054A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CU1988200A CU22054A1 (en) 1988-12-12 1988-12-12 Method for purifying inhibitors of the sea anemonestaichactis helianthus proteases and the product thus obtained.
SU894763976A RU1789525C (en) 1988-12-12 1989-11-28 Method of protease inhibitors purification from sea anemone

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1988200A CU22054A1 (en) 1988-12-12 1988-12-12 Method for purifying inhibitors of the sea anemonestaichactis helianthus proteases and the product thus obtained.

Publications (1)

Publication Number Publication Date
CU22054A1 true CU22054A1 (en) 1993-04-30

Family

ID=5459533

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1988200A CU22054A1 (en) 1988-12-12 1988-12-12 Method for purifying inhibitors of the sea anemonestaichactis helianthus proteases and the product thus obtained.

Country Status (2)

Country Link
CU (1) CU22054A1 (en)
RU (1) RU1789525C (en)

Also Published As

Publication number Publication date
RU1789525C (en) 1993-01-23

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