CS274966B2 - Method of general-purpose chromolytic substrate preparation for proteinasa's enzyme activity determination - Google Patents
Method of general-purpose chromolytic substrate preparation for proteinasa's enzyme activity determinationInfo
- Publication number
- CS274966B2 CS274966B2 CS540288A CS540288A CS274966B2 CS 274966 B2 CS274966 B2 CS 274966B2 CS 540288 A CS540288 A CS 540288A CS 540288 A CS540288 A CS 540288A CS 274966 B2 CS274966 B2 CS 274966B2
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- weight
- percent
- egg white
- stage
- proteinases
- Prior art date
Links
- 239000000758 substrate Substances 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 abstract 3
- 102000002322 Egg Proteins Human genes 0.000 abstract 3
- 108010000912 Egg Proteins Proteins 0.000 abstract 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- 102000035195 Peptidases Human genes 0.000 abstract 3
- 108091005804 Peptidases Proteins 0.000 abstract 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- 235000014103 egg white Nutrition 0.000 abstract 3
- 210000000969 egg white Anatomy 0.000 abstract 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 239000007795 chemical reaction product Substances 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 abstract 2
- 235000019833 protease Nutrition 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- URPBSRQSVAMQRT-UHFFFAOYSA-N 4-amino-9,10-dioxo-1-[3-(2-sulfoethylsulfonyl)anilino]anthracene-2-sulfonic acid Chemical compound NC=1C=C(C(=C2C(C=3C=CC=CC3C(C12)=O)=O)NC1=CC(=CC=C1)S(=O)(=O)CCS(=O)(=O)O)S(=O)(=O)O URPBSRQSVAMQRT-UHFFFAOYSA-N 0.000 abstract 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 abstract 1
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000004040 coloring Methods 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 235000013372 meat Nutrition 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 229960005382 phenolphthalein Drugs 0.000 abstract 1
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 238000010561 standard procedure Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Whole egg white, without preliminary treatment is cross-linked at a temperature of 15 to 25 degrees C in the first stage by epichlorhydrine in quantity of 3 to 5 percent by weight per the weight of egg white dry matter and is coloured by colouring agent Remazol Brillant Blue R (disodium salt of 8-amino-5-[3-(sulfoethylsulfonyl)anilino]6-anthraquinone sulfonic acid in the amount of 8 to 12 percent by weight per the weight of egg white dry matter in alkaline environment in concentration of 0.9 to 1.3 percent by weight of sodium hydroxide for a period of 12 to 16 hours. In the second stage the reaction product is defibred in mixer in such a volume of ethanol so that its resulting concentration in the reaction product would be 50 percent by weight. After mixing the chromological substrate is washed on filter with distilled water for a period of time necessary for the filtrate to be colourless and for the phenol phthalein not to colour in red. In the third stage water is displaced by ethanol, with 0.5 percent by weight of previously dissolved sodium chloride. Finally the gel is dried of methanol, ground on particles with dimensions ranging from 0.06 to 0.35 micrometers using standard method.The solution can be used in analytics of proteinase enzymes, selection of microorganisms for production of proteinases, interoperation monitoring of production of proteinases and in meat contamination control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS540288A CS274966B2 (en) | 1988-08-02 | 1988-08-02 | Method of general-purpose chromolytic substrate preparation for proteinasa's enzyme activity determination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS540288A CS274966B2 (en) | 1988-08-02 | 1988-08-02 | Method of general-purpose chromolytic substrate preparation for proteinasa's enzyme activity determination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS8805402A1 CS8805402A1 (en) | 1990-10-12 |
| CS274966B2 true CS274966B2 (en) | 1991-12-17 |
Family
ID=5398869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS540288A CS274966B2 (en) | 1988-08-02 | 1988-08-02 | Method of general-purpose chromolytic substrate preparation for proteinasa's enzyme activity determination |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS274966B2 (en) |
-
1988
- 1988-08-02 CS CS540288A patent/CS274966B2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS8805402A1 (en) | 1990-10-12 |
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