CS274798B2 - Grained immobilized biocatalyst with invertase activity for saccharose's enzymatic hydrolysis - Google Patents
Grained immobilized biocatalyst with invertase activity for saccharose's enzymatic hydrolysisInfo
- Publication number
- CS274798B2 CS274798B2 CS109190A CS109190A CS274798B2 CS 274798 B2 CS274798 B2 CS 274798B2 CS 109190 A CS109190 A CS 109190A CS 109190 A CS109190 A CS 109190A CS 274798 B2 CS274798 B2 CS 274798B2
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- water solution
- saccharose
- solid phase
- enzymatic hydrolysis
- biocatalyser
- Prior art date
Links
- 229930006000 Sucrose Natural products 0.000 title abstract 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 title abstract 3
- 235000013681 dietary sucrose Nutrition 0.000 title abstract 3
- 229960004793 sucrose Drugs 0.000 title abstract 3
- 230000000694 effects Effects 0.000 title abstract 2
- 230000007071 enzymatic hydrolysis Effects 0.000 title abstract 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 title 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 title 1
- 239000011942 biocatalyst Substances 0.000 title 1
- 235000011073 invertase Nutrition 0.000 title 1
- 239000001573 invertase Substances 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 6
- 238000006243 chemical reaction Methods 0.000 abstract 3
- 239000007790 solid phase Substances 0.000 abstract 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 abstract 1
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 abstract 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 abstract 1
- 241000235070 Saccharomyces Species 0.000 abstract 1
- 241000582914 Saccharomyces uvarum Species 0.000 abstract 1
- 235000021120 animal protein Nutrition 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 229920000768 polyamine Polymers 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
- 235000011164 potassium chloride Nutrition 0.000 abstract 1
- 239000001103 potassium chloride Substances 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 abstract 1
- 238000007864 suspending Methods 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 210000005253 yeast cell Anatomy 0.000 abstract 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The solution concerns a granular immobilised biocatalyser with invertasic activity for enzymatic hydrolysis of saccharose to D-glucose and D-fructose in water solutions of various origin and purity degree in continuous flow reactors and immovable bed of biocatalyser particles on the one hand, and in the mixed continuous and batch reactors on the other. The catalyser can be prepared by reaction of suspension of yeast cells of Saccharomyces corovisina or Saccharomyces uvarum in water solution of microbial or animal protein or in water solution of synthetic polyamine or polyimine with water solution of reactive soluble polymer prepared by chemical reaction of water solution of a compound containing in one molecule at least two primary or secondary amino groups with dialdehyde of dicarboxylic acid, or eventually by subsequent reaction created cellular aggregates with glutaraldehyde, by separation of solid phase by filtration or centrifugation, suspending of the solid phase in water solution of saccharose or inorganic salts, optimally potassium chloride, repeated separation of solid phase, forming of particles of suitable shape and size and finally their drying on air at a temperature up to +60 degrees C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS901091A CS109190A2 (en) | 1990-03-06 | 1990-03-06 | Grained immobilized biocatalyst with invertase activity for saccharose's enzymic hydrolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS901091A CS109190A2 (en) | 1990-03-06 | 1990-03-06 | Grained immobilized biocatalyst with invertase activity for saccharose's enzymic hydrolysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS274798B2 true CS274798B2 (en) | 1991-11-12 |
| CS109190A2 CS109190A2 (en) | 1991-11-12 |
Family
ID=5344377
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS901091A CS109190A2 (en) | 1990-03-06 | 1990-03-06 | Grained immobilized biocatalyst with invertase activity for saccharose's enzymic hydrolysis |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS109190A2 (en) |
-
1990
- 1990-03-06 CS CS901091A patent/CS109190A2/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CS109190A2 (en) | 1991-11-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| IF00 | In force as of 2000-06-30 in czech republic | ||
| MM4A | Patent lapsed due to non-payment of fee |
Effective date: 20030306 |