CS273414B1 - Mechanical arrangement of immobilized enzyme's carrier - Google Patents
Mechanical arrangement of immobilized enzyme's carrier Download PDFInfo
- Publication number
- CS273414B1 CS273414B1 CS794988A CS794988A CS273414B1 CS 273414 B1 CS273414 B1 CS 273414B1 CS 794988 A CS794988 A CS 794988A CS 794988 A CS794988 A CS 794988A CS 273414 B1 CS273414 B1 CS 273414B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- enzyme
- carrier
- analysis
- immobilized enzyme
- mechanical arrangement
- Prior art date
Links
- 108010093096 Immobilized Enzymes Proteins 0.000 title claims description 6
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 239000000126 substance Substances 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 4
- 239000004744 fabric Substances 0.000 description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Vynález se týká mechanického uspořádání nosiče imobilizovaného enzymu.The invention relates to the mechanical arrangement of the immobilized enzyme support.
Využití enzymů i celých biochemických systémů v analytické chemii přináší výrazný pokrok daný neobyčejné vhodnými vlastnostmi enzymů pro tuto aplikační sféru.The use of enzymes and whole biochemical systems in analytical chemistry brings significant progress due to the extraordinary suitable properties of enzymes for this application sphere.
Průběh těchto reakcí je možno sledovat buá následnými chemickými reakcemi vzniklých reakčních produktů, nebo s výhodou fyzikálně - chemickými metodami. 3edna z možností je sledovat reakce, při kterých dochází ke změnám koncentrace kyslíku, peroxidu vodíku, oxidu uhličitého, NAD/P/H, H+, NH^+, popřípadě dalších iontů.The course of these reactions can be monitored either by subsequent chemical reactions of the resulting reaction products, or preferably by physico-chemical methods. One of the options is to monitor reactions that change the concentration of oxygen, hydrogen peroxide, carbon dioxide, NAD / P / H, H + , NH 4 + , and possibly other ions.
Typ aplikovaného enzymu určuje i druh použitelného elektrochemického čidla. Nejběžnější je Clarkovo kyslíkové čidlo, dále se používají skleněné elektrody na měření pH, iontově selektivní elektrody, plynové elektrody a čidla ISFET.The type of enzyme applied determines the type of electrochemical sensor to be used. Clark's oxygen sensor is the most common, glass electrodes for pH measurement, ion-selective electrodes, gas electrodes and ISFET sensors are also used.
Použití volných enzymů naráží v některých případech na technické nebo ekonomické problémy jejich získávání, obtížnou regeneraci z reakční směsi, atd. Z těchto důvodů je velmi aktuální imobilizace enzymů a následná fixace v blízkosti elektrochemického čidla, čímž se získá biosenzor opakovaně použitelný při stovkách analýz bez jakékoliv spotřeby chemikálií.The use of free enzymes in some cases encounters technical or economic problems of their recovery, difficult recovery from the reaction mixture, etc. For these reasons, enzyme immobilization and subsequent fixation in the vicinity of the electrochemical sensor are very timely, making biosensor reusable in hundreds of analyzes without any consumption of chemicals.
jednou z možností je fixovat imobilizovaný enzym na polyamidové tkanině, kterou je třeba dobře vypnout na pracovní plochu elektrochemického čidla tak, aby prostor mezi tkaninou a separační membránou čidla byl co nejtenčí a neměnný a aby nedocházelo k posunům tkaniny při hydraulických rázech při míchání měřeného roztoku. Běžně užívaný způsob, kdy tkanina s imobilizovaným enzymem se spolu s například separační membránou Clarkova kyslíkového čidla upne najednou na čidlo jedním pružným prstencem, je poněkud nevýhodný. Za prvé je tkanina velmi jemná a manipulace s ní jak při imobilizaci enzymu na ní, tak při upínání na čidlo je obtížná. Za druhé je životnost tkaniny s enzymem jiná než životnost separační membrány a vnitřního elektrolytu kyslíkového čidla, a je zbytečné při výměně tkaniny s enzymem měnit separační membránu a vnitřní elektrolyt kyslíkového čidla.One possibility is to fix the immobilized enzyme on the polyamide fabric, which should be well turned off on the working surface of the electrochemical sensor so that the space between the fabric and the sensor separation membrane is as thin and unchangeable as possible. A commonly used method in which a fabric with immobilized enzyme, together with, for example, the separation membrane of a Clark oxygen sensor, is clamped to the sensor at the same time by a single elastic ring, is somewhat disadvantageous. First, the fabric is very delicate and is difficult to handle when immobilizing the enzyme thereon and attaching it to the sensor. Second, the life of the enzyme fabric is different from that of the separation membrane and the internal electrolyte of the oxygen sensor, and it is unnecessary to change the separation membrane and the internal electrolyte of the oxygen sensor when replacing the enzyme fabric.
Uvedené nedostatky odstraňuje mechanické uspořádání nosiče imobilizovaného enzymu, jehož podstatou je, že vlastní nosič 1. enzymu - peLyamidová tkanina je přivařena termokompresí na rámeček 2^ jehož tvar je přizpůsoben danému elektrochemickému čidlu. Rámeček se nasune na čidlo a spolu s čidlem se upne do bloku měřící cely. Materiál rámečku 2 je polypropylen. Při nahřátí jeho konce 2 na danou teplotu a přitlačením určitou silou na vypnutou tkaninu nosiče 1_ dojde k.protečení materiálu rámečku 2 otvory ve tkanině a při zpětném ochlazení je tkanina nosiče 1^ pevně spojena s rámečkem 2.These drawbacks are eliminated by the mechanical arrangement of the immobilized enzyme carrier, which is based on the fact that the enzyme carrier 1 - polyamide fabric is welded by thermocompression to a frame 2 whose shape is adapted to the electrochemical sensor. The frame is put on the sensor and together with the sensor is clamped into the measuring cell block. Frame 2 material is polypropylene. By heating its end 2 to a given temperature and pressing it with a certain force on the fabric of the carrier 1 which is switched off, the material of the frame 2 flows through the holes in the fabric and upon cooling back the fabric of the carrier 1 is firmly connected to the frame 2.
Další výhodou tohoto systému je snadná a rychlá výměna rámečku s daným enzymem za jiný, a tím alternativní provádění analýz více látek.Another advantage of this system is the easy and quick replacement of the frame with a given enzyme for another, and thus an alternative multi-substance analysis.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS794988A CS273414B1 (en) | 1988-12-05 | 1988-12-05 | Mechanical arrangement of immobilized enzyme's carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS794988A CS273414B1 (en) | 1988-12-05 | 1988-12-05 | Mechanical arrangement of immobilized enzyme's carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS794988A1 CS794988A1 (en) | 1990-07-12 |
| CS273414B1 true CS273414B1 (en) | 1991-03-12 |
Family
ID=5429483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS794988A CS273414B1 (en) | 1988-12-05 | 1988-12-05 | Mechanical arrangement of immobilized enzyme's carrier |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS273414B1 (en) |
-
1988
- 1988-12-05 CS CS794988A patent/CS273414B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS794988A1 (en) | 1990-07-12 |
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